Cyperus Rotundus Research Articles

Enzyme inhibitory potency of nano formulation of some plant oils on the red palm weevil Rhynchophorus ferrugineus olivier

Inhibitory effect of the tested plant oils and their nanoformulation against trypsin and chymotrypsin enzyme extracted from Rhynchophorus ferrugineus, was studies in in vivo and in vitro. In vivo inhibition, the reduction in the trypsin specific activity caused by the bulk oils was greater than that of chymotrypsin enzyme. Compared to control nanoformulation caused significant reduction in the specific activity of trypsin enzyme; however, there were no inhibition effect observed on chymotrypsin enzyme. In vitro bulk oils showed very low inhibitory effect against trypsin enzyme, while they showed strong inhibition activity against chymotrypsin with recording for ginger nanoformulation (half-maximal inhibitory concentration (IC50) =6.83 μg/ml), purple nutsedge (7.632 μg/ml), garden cress (11.52 μg/ml) and castor (16.82 μg/ml), nanoformulation of these oils showed uncompetitive type of inhibition as the Vmax and Km values changed compared to control. In conclusion, nanoformulation of the tested plant oils could be used in the integrated pest management (IPM) programs of the red palm weevil (RPW).

Unveiling the Allelopathic Potential of Wedelia Leaf Extract as a Bioherbicide against Purple Nutsedge: A Promising Strategy for Sustainable Weed Management

Weed management is a crucial aspect of sustainable agriculture. In this study, we investigated the allelopathic potential of wedelia (Wedelia trilobata L.) leaf extract as a bioherbicide against purple nutsedge (Cyperus rotundus L.). The experiments were carried out through greenhouse experiments using a completely randomized design (CRD) with four replications. Five different concentrations were evaluated: C10% (10% wedelia extract concentration), C20% (20% wedelia extract concentration), C40% (40% wedelia extract concentration), C+ (92 mg L−1 of gallic acid), and C− (aquadest). Allelochemicals present in the wedelia leaf extract inhibited plant height, shoot number, leaf number, leaf area, root area, and total root length. The fresh weight, dry weight, and photosynthetic pigments decreased with increasing wedelia leaf extract concentrations. Malondialdehyde contents were highest when C40% was used. Additionally, peroxide activities decreased at the highest wedelia leaf extract concentration, indicating the failure of the plant’s antioxidant defense mechanism. The decrease in growth, photosynthetic pigment, and antioxidant activity indicates that wedelia leaf extract may be able to help control the growth of purple nutsedge. The results of this study could contribute to the development of a new cropping system based on the use of wedelia as a bioherbicide for sustainable agriculture.

Diterpene alcohol fraction of Cyperus rotundus Linn essential oil regulates Bcl-2 and Bax expression inducing apoptosis on HeLa in vitro and in silico

Apoptosis, or programmed cell death, is a crucial mechanism in preventing cancer growth, and one of the targets is reducing the expression of the anti-apoptotic protein Bcl-2. This study aimed to evaluate the potential of fractions from nutsedge (Cyperus rotundus) essential oil in inducing apoptosis and inhibiting the progression of cervical cancer. The researchers investigated the apoptosis-inducing activity of these fractions against the HeLa cervical cancer cell line using in vitro and in silico approaches. The cytotoxic effects were assessed through an MTT assay on HeLa cells cultured in 96-well plates. Additionally, flow cytometry with Annexin/PI staining was employed to analyze the induction of apoptosis by the fractions. The immunocytochemical staining of cells was also implemented to assess Bax and Bcl-2 expressions. The biologically active compound in fractions was screened using a molecular docking approach to Bcl-2 co-crystalized structure and their pharmacokinetics and toxicity profile were assessed. The cytotoxic activity differed significantly from each fraction. The highest cytotoxicity was observed in fraction 1 (IC50: 8.307 + 0.186 mcg/ml), and the lowest cytotoxicity was observed in fraction 4 (IC50:>500 mcg/ml). Fraction 1 decreased the Bcl-2 expression and increased the Bax expression. Molecular docking screening revealed that 5-(7a-isopropenyl-4,5-dimethyl-octahydro-inden-4-yl)-3-methyl-pent-2-en-1-ol was predicted as the main contributor to apoptosis-inducing activity of fraction 1. The supplementation of fraction 1 induces cell apoptosis on HeLa cells, indicating the potential of this fraction of nutsedge essential oil for developing an anti-cervical cancer agent.

Antibacterial activity of five Indonesian medicinal plants and the isolation of compounds from Plectranthus scutellarioides

The purpose of this study is to investigate the antibacterial properties of five Indonesian medicinal plant extracts, namely Physalis angulata, Loranthus parasiticus, Plectranthus scutellarioides, Cyperus rotundus, and Terminalia catappa. The isolation of compounds from P. scutellarioides against pathogenic bacteria was also investigated. The assessment of the antibacterial activity of the extracts was conducted using the microdilution-3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide method against Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli). The plant extract with the highest antibacterial activity was further purified using various chromatographic assays to isolate bioactive compounds. High Resolution-Electron Ionization Mass Spectroscopy/Electrospray Ionization Mass Spectroscopy (HR-ESIMS/EIMS) and Nuclear Magnetic Resonance (NMR) spectrometers were used to elucidate the structure of isolated compounds. Among all the plants tested, P. scutellarioides showed the strongest antibacterial activity against S. aureus [minimum inhibitory concentration (MIC 100 μg/ml)] and B. subtilis (MIC 200 μg/ml). Three compounds were isolated from P. scutellarioides including an abietane diterpene, 2,16-diacetoxy-6,11,12,14,17-pentahydroxy-abieta-5,8,11,13- tetraene-7-one (1), 5,6,7,3′,4′,5′-hexamethoxy flavone (2), and 5,6,7,8,3′,4′,5′-heptamethoxy flavone (3). Compounds 2 and 3 were recognized to be first isolated from this plant. Compound 1 exerted antibacterial activity against S. aureus with a MIC value of 60 μM, but was inactive against B. subtilis and E. coli (MIC > 200 μM). Compounds 2 and 3 were inactive against S. aureus, B. subtilis, and E. coli (MIC > 200 μM).

Nanoparticle Encapsulation of the Hexane Fraction of Cyperus Rotundus Extract for Enhanced Antioxidant and Anti-Inflammatory Activities in vitro.

Cyperus rotundus L. (CR) is traditionally used in medicine for its anti-inflammatory properties. In particular, α-cyperone, which is isolated from the essential oil and found primarily in the n-hexane fraction of the ethanolic extract, is known to inhibit NO production in LPS-stimulated RAW 264.7 cells. However, high concentrations of α-cyperone are required for sufficient anti-inflammatory activity. Even, essential oil obtained from C. rotundus has the disadvantage of low solubility and stability in aqueous environment, which makes it difficult to be applied in various fields and easily loses its activity. Therefore, in this study, we aimed to increase the extraction yield of C. rotundus by microbubble extraction and prepare nanoparticles (NPs) that can preserve its activity in a stable and bioavailable manner by utilizing nanoprecipitation. C. rotundus rhizomes were extracted in 50% ethanol using microbubbles and then fractionated with n-hexane to obtain α-cyperone-rich C. rotundus n-hexane fraction (CRHF). The biodegradable plant extract, α-cyperone, was prepared as green nanoparticles (CR@NPs) by nanoprecipitation technique under mild reaction conditions. The physicochemical properties of CR@NPs, including size, polydispersity index, and surface charge, were determined using dynamic light scattering. The extraction yield and encapsulation efficiency of α-cyperone were quantified by high-performance liquid chromatography. Antioxidant and anti-inflammatory activities were evaluated by DPPH assay and in vitro ROS and NO assays, and biocompatibility was assessed by MTT assay. C. rotundus loaded nanoparticles demonstrated overcoming the limitation of α-cyperone solubility and stability in CRHF and also the antioxidant, anti-inflammatory properties as evidenced by in vitro assays in cellular models. The versatility of green chemistry, such as α-cyperone, enables the production of nanoparticles with promising biomedical applications such as cosmetics, pharmaceuticals, and food products.

Citrullus colocynthis fruit resin and Cyperus rotundus tuber resin: Assessing their analgesic effects with hot plate and writhing tests

Citrullus colocynthis fruit resin and Cyperus rotundus tuber resin: Assessing their analgesic effects with hot plate and writhing tests

In silico approach for predicting the bioactive compound of Cyperus rotundus to inhibit NF-kB and iNOS signaling pathways.

This study aims to evaluate the anti-cancer-related inflammation activity of Cyperus rotundus bioactive compounds. The component of C. rotundus was analyzed using LC-HRMS. The drug-likeness of all compounds were analyzed using swissADME webserver. In addition, the analysis of inhibition potential of compounds against NF-κB and iNOS were carried out using molecular docking in PyRx software. This study found 1-Nitro-2-phenoxybenzene, ethyl 4-(acetylamino)-3-phenyl-2-thioxo-2,3-dihydro-1,3-thiazole-5-carboxylate, and nootkatone passed all the parameters of drug-likeness including Lipinski, ghose, veber, egan, and muege. Based on molecular docking, verbascoside A and n-Pentyl isopentyl phthalate has the lowest binding affinity against iNOS (-10 and -8.9 kcal/mol, respectively). In addition, verbascoside A and maltopentaose have binding affinity of -7.6 and -6.6 kcal/mol, respectively, for NF-κB. The anti-cancer activity of verbascoside A, maltopentaose, and n-Pentyl isopentyl phthalate, according to PASS analysis were anti-inflammatory, antineoplastic, chemopreventive, and chemoprotectant. The cytotoxic effect prediction showed that these compounds were relatively selective to kill tumor cell but not non-tumor cell. Rat toxicity analysis showed maltopentaose was non-toxic, where n-Pentyl isopentyl phthalate was only toxic (class IV) for intravenous administration. perMM analysis showed verbascoside A and n-Pentyl isopentyl phthalate can translocate and across the cell membrane.

Condicionamento de sementes com qualidade de luz e extrato de Cyperus rotundus L. modula a germinação e o crescimento inicial de plântulas de Moringa oleifera

Improving plant germination is essential to guarantee better quality seedlings. Thus, this research aimed to evaluate whether the seed priming with light quality (LIQ) and the aqueous extract of Cyperus rotundus (AEC) tuber could modulate the germination and initial growth of Moringa oleifera L. seedlings. The experimental design was a completely randomized in the 4x4 factorial scheme, composed of four LIQ conditions (white, blue, red, and distant red light) and four AEC concentrations (0, 25, 50 and 100%). Seed priming with red light reduced the average emergence time, while blue, red, and extreme red lights associated with 50% of aqueous extract of C. rotundus increased shoot initial length and photosynthetic pigment accumulation. Seed priming with blue light resulted in seedlings with a shorter final shoot length. However, application of 100% of aqueous extract of C. rotundus reversed this. The white light in combination with concentrations of 50 and 100% of AEC promoted a higher relative shoot growth rate of seedlings. The research revealed that seed priming with light quality and aqueous extracts of C. rotundus tubers modulates the germination and initial growth of M. oleifera seedlings. More work needs to be done to determine the responsible compounds in AEC that is responsible for priming growth as phytohormones.

The Herbal Combination Shu Gan Jie Yu Regulates the SNCG/ER-a/AKT-ERK Pathway in DMBA-Induced Breast Cancer and Breast Cancer Cell Lines Based on RNA-Seq and IPA Analysis.

Soothing the liver (called Shu Gan Jie Yu in Chinese, SGJY) is a significant therapeutic method for breast cancer in TCM. In this study, 3 liver-soothing herbs, including Cyperus rotundus L., Citrus medica L. var. sarcodactylis Swingle and Rosa rugosa Thunb. were selected and combined to form a SGJY herbal combinatory. To investigate the inhibiting effect of SGJY on breast cancer in vivo and vitro, and to explore the potential mechanisms. SGJY herbal combination was extracted using water. A breast cancer rat model was developed by chemical DMBA by gavage, then treated with SGJY for 11 weeks. The tumor tissue was preserved for RNA sequencing and analyzed by IPA software. The inhibition effects of SGJY on MCF-7 and T47D breast cancer cells were investigated by SRB assay and cell apoptosis analysis, and the protein expression levels of SNCG, ER-α, p-AKT and p-ERK were measured by western blotting. SGJY significantly reduced the tumor weight and volume, and the level of estradiol in serum. The results of IPA analysis reveal SGJY upregulated 7 canonical pathways and downregulated 16 canonical pathways. Estrogen receptor signaling was the key canonical pathway with 9 genes downregulated. The results of upstream regulator analysis reveal beta-estradiol was the central target; the upstream regulator network scheme showed that 86 genes could affect the expression of the beta-estradiol, including SNCG, CCL21 and MB. Additionally, SGJY was verified to significantly alter the expression of SNCG mRNA, CCL21 mRNA and MB mRNA which was consistent with the data of RNA-Seq. The inhibition effects of SGJY exhibited a dose-dependent response. The apoptosis rates of MCF7 and T47D cells were upregulated. The protein expression of SNCG, ER-α, p-AKT and p-ERK were all significantly decreased by SGJY on MCF-7 and T47D cells. The results demonstrate that SGJY may inhibit the growth of breast cancer. The mechanism might involve downregulating the level of serum estradiol, and suppressing the protein expression in the SNCG/ER-α/AKT-ERK pathway.

The Manual, Chemical, Cultural, and Integrated Weed Management in Soybean Production

A field experiment was conducted at Sher-e-Bangla Agricultural University, Dhaka, from December 2021 to April 2022 to study the effect of weed management practices in soybean (Glycine max L.). The experiment was laid out in a Randomized three-Complete Block Design (RCBD) with twelve weed management treatments- no weeding (Control), two hand weeding (at 15 and 30 DAS), pre-emergence herbicide (Herbilin 33% EC @ 400 ml ha-1), post-emergence herbicide Irish EC @ 1200 ml ha-1, pre + post-emergence herbicide, pre-emergence + 1 hand weeding {40 days after sowing (DAS)}, post-emergence herbicide + 1 hand weeding (40 DAS), pre + post-emergence herbicide + 1 hand weeding(40 DAS), straw mulching, intercrop with Amaranthus dubius, and Zea mays, and weed-free. The experimental result showed among seven different weed species found, Cyperus rotundus was the most prevalent weed, with the highest weed density (123 and 128.67 m-2) and relative weed emergence (43.16 and 38.79 %) in the control plot at 30 and 60 DAS. The treatment pre + post-emergence herbicide + 1 hand weeding (40 DAS) showed the longest pod length (4.16 cm) and number of seeds pod-1 (3.89). The weed-free treatment resulted in the highest 1000-seed weight (111.00 g), seed yield (1.86 t ha-1), stover yield (2.16 t ha-1), biological yield (4.03t ha-1), and harvest index (46.35 %). However, the highest benefit-cost ratio (2.85) was obtained under post-emergence herbicide (Irish EC) treatment. Therefore, applying Irish EC was the best broad-spectrum effective herbicide to manage the various weed floras in soybeans for profit. SAARC J. Agric., 21(2): 195-206 (2023)

Formulation and Evaluation of Ointment Preparations with Nutgrass Extract (Cyperus rotundus L.) Using Different Ointment Bases

Formulation and Evaluation of Ointment Preparations with Nutgrass Extract (Cyperus rotundus L.) Using Different Ointment Bases

Identification of Ergastic Subtances of The Order Poales

Ergastic objects are one of the non-protoplasmic components besides vacuoles. Ergastic objects in protoplasts can be solid and liquid.The purpose of the study was to determine how the structure and type of ergastic substances in plants of order poales. This research method includes descriptive research that describes the type and structure of ergastic substances in plants of order poales, the parts observed are bulbs, grains, stems to fruits that contain ergasticsubstances.Based on the results of research on the order of poales found that the order of poales has a diverse type of solid ergastics, such as amylum (starch) found in teapot grass plants (Cyperus rotundus), rice (Oryza sativa) and corn (Zea mays) with various shapes and types, there are also types of ergastic substances in the form of ca-oxalate crystals found in pineapple plants (Ananas comusus) and types of sugar-shaped egastic substances in sugar cane plants (Saccharum officinarum Linn).

Weed Management Practices on Growth, Yield and Economics of Kharif Grain Sorghum

In the Kharif grain sorghum growing areas of Karnataka, the sorghum production suffers greatly due to weed problem, which offers limitations to crop. It was found that weed infestation become unmanageable throughout the growing period by the traditional methods of interculturing and manual weeding due to continuous and heavy rains during entire vegetative and early reproductive stages of Kharif sorghum. Hence, integration of herbicides with some cultural operations and use of pre-emergence, post-emergence herbicides in combination with mechanical methods can prove to be more successful. A field experiment entitled “Weed management in kharif grain sorghum” was conducted during Kharif 2019 at ARS, Hagari in deep black soil with neutral reaction (pH 7.50), organic carbon (5.5 g kg-1), available nitrogen (248.00 kg ha-1), available phosphorus (36.75 kg ha-1) and available potassium (312.00 kg ha-1). An experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. There were fourteen treatments comprising of weed management practices. The dominant weeds observed in the experimental fields were among grasses Brachiaria reptans, while in broad leaved weeds, Amaranthus viridis and among sedges, Cyperus rotundus. Significantly lower population of grasses, sedges, and broad leaved weeds, weed dry weight, weed index (%) and higher weed control efficiency throughout the crop growth period was noticed in sequential pre-emergence application of atrazine 50 WP @ 0.50 kg a.i. ha-1 as PE fb 2,4-D Na Salt 80 WP @ 0.75 kg a.i. ha-1 as PoE at 25 DAS except weed free treatment. Atrazine 50 WP @ 0.50 kg a.i. ha-1 as PE fb 2,4-D Na Salt 80 WP @ 0.75 kg a.i. ha-1 as PoE at 25 DAS recorded significantly higher plant height, dry matter accumulation, leaf area, test weight, grain yield, straw yield, harvest index, gross return, net return and benefit cost ratio (160.8 cm, 183.4 gplant-1, 24.1 dm2 plant-1, 30.53 g, 4195 kg ha-1, 9891 kg ha-1, 29.80 %, Rs. 1,03,675ha-1, Rs. 71,636 ha-1and 3.24, respectively) except weed free and it was on par with atrazine 50 WP @ 0.50 kg a.i. ha-1 as PE fb IC at 25 DAS and HW at 25 DAS and IC at 40 DAS.

Weed management strategies in elephant foot yam (<i>Amorphophallus paeoniifolius</i>) under different agro environments in India

In elephant foot yam [Amorphophallus paeoniifolius (Dennst.) Nicolson], weeds are the major constraints cause yield reduction up to 100% due to its very slow initial sprouting, establishment and plant growth. Information on proper weed management in elephant foot yam in India is limited as its commercial cultivation started very recently. For arriving at suitable and better weed control, field experiments were conducted for 3 growing seasons (2019 to 2021) at ICAR-Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala with 8 treatments in 6 different agro-climatic zones of India. The treatments included a combination of pre emergence (PE) and post emergence (POE) herbicides, PE followed by hand weeding (HW), intercropping followed by POE, HW with POE, POE alone, and weed control ground cover cloth mulch (WCGCC), three HW, and a weedy check were included for comparison. Weed flora was dominated by broad-leaf weeds, Digera arvensis (L.), Commelina benghalensis (L.); grasses, Pennisetum pedicellatum Trin., Cynodon dactylon (L.) pers.; and the sedge, Cyperus rotundus (L.), in most of the locations. Pooled analysis of data collected over different locations indicated that the lower mean weed biomass (21.24 g/m) and higher mean corm yield (35.13 t/ha), higher mean net income (`0.326 million/ha) were recorded with weed control ground cover mulch and higher mean B:C ratio (2.39) by application of post emergence herbicide at 30, 60 and 90 days after planting (DAP).

Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater

Better understanding of macrophyte tolerance under long exposure times in real environmental matrices is crucial for phytoremediation and phytoattenuation strategies for aquatic systems. The metal(loid) attenuation ability of 10 emergent macrophyte species (Carex riparia, Cyperus longus, Cyperus rotundus, Iris pseudacorus, Juncus effusus, Lythrum salicaria, Menta aquatica, Phragmites australis, Scirpus holoschoenus, and Typha angustifolia) was investigated using real groundwater from an industrial site, over a 90-day exposure period. A “phytobial” treatment was included, with 3 plant growth-promoting rhizobacterial strains. Plants exposed to the polluted water generally showed similar or reduced aerial biomass compared to the controls, except for C. riparia. This species, along with M. aquatica, exhibited improved biomass after bioaugmentation. Phytoremediation mechanisms accounted for more than 60% of As, Cd, Cu, Ni, and Pb removal, whilst abiotic mechanisms contributed to ∼80% removal of Fe and Zn. Concentrations of metal(loid)s in the roots were generally between 10–100 times higher than in the aerial parts. The macrophytes in this work can be considered “underground attenuators”, more appropriate for rhizostabilization strategies, especially L. salicaria, M. aquatica, S. holoschoenus, and T. angustifolia. For I. pseudacorus, C. longus, and C. riparia; harvesting the aerial parts could be a complementary phytoextraction approach to further remove Pb and Zn. Of all the plants, S. holoschoenus showed the best balance between biomass production and uptake of multiple metal(loid)s. Results also suggest that multiple phytostrategies may be possible for the same plant depending on the final remedial aim. Phytobial approaches need to be further assessed for each macrophyte species.

Exploring the Antimicrobial Potential of Cyperus rotundus Extract Against Multidrug-Resistant Escherichia coli in Urinary Tract Infections

The widespread use of antibiotics has contributed to the emergence of antibiotic-resistant bacteria, including Escherichia coli (E. coli), a primary cause of urinary tract infections (UTIs). The increasing antimicrobial resistance levels have prompted research into alternative treatments, such as medicinal plants, to counter this global health concern. This study aimed to evaluate the effectiveness of solvent extracts from Cyperus rotundus (C. rotundus) against multidrug-resistant strains of E. coli associated with UTIs, as well as examine the phytochemical characteristics of the plant extract. E. coli isolates from pregnant women with UTIs were collected from several clinical laboratories, and their antibiotic resistance levels were evaluated. The antimicrobial activity of the C. rotundus extract was assessed using the agar-well diffusion method. In addition, GC-MS analysis was employed to screen for qualitative phytochemicals and secondary metabolites. The C. rotundus extract demonstrated significant inhibitory results on the resistant isolates, with a zone of inhibition ranging from 9mm to 17mm. The extract was found to contain various phytochemicals such as alkaloids, flavonoids, phenolics, tannins, and triterpenoids. GC-MS analysis detected bioactive compounds such as 3, 4-Altrosan, Phytol, Isoquinoline, and beta-sitosterol. These findings show that C. rotundus has the potential to be a valuable resource in the fight against antibiotic-resistant UTIs, underscoring the need for further investigation.

Cyperus rotundus L.: Invasive weed plant with insecticidal potential against Aphis craccivora Koch and Planococcus lilacinus (Cockerell)

Cyperus rotundus L. is a widely distributed invasive weed plant with vast traditional medicinal uses. Herein, the methanolic root extract of C. rotundus and its fractions (n-hexane, chloroform, n-butanol, and aqueous) were evaluated for insecticidal activity against nymphs of Aphis craccivora Koch and crawlers of Planococcus lilacinus (Cockerell) to find promising lead (s). In contact topical assay, among extract/fractions, n-hexane fraction exhibited more toxicity against A. craccivora (LD50 = 1.12 μg/insect) and P. lilacinus (LD50 = 0.94 μg/insect). The chemical analysis of n-hexane fraction revealed a volatile composition similar to that of the essential oil (EO) of C. rotundus roots. Hence, EO was extracted using water and deep eutectic solvents (DESs) as cosolvent, which revealed enhancement in EO yield (from 0.28 to 0.46% w/w) on implementing DESs. A total of 35 diverse volatile metabolites were identified in all EO samples, accounting for 85.0 to 91.8% of chemical composition, having cyperotundone, cyperene mustakone, isolongifolen-5-one, boronia butenal as major constituents. The EO obtained with DES-7 [choline chloride: ethylene glycol (1:4)] and DES-6 [choline chloride: lactic acid (1:3)] were found effective against A. craccivora (LD50 = 0.62–0.87 μg/insect) and P. lilacinus (LD50= 0.59–0.67 μg/insect) after 96 h. NMR analysis of EO revealed cyperotundone as a major compound, which was isolated along with cyperene and cyperene epoxide. All the molecules were found effective against P. lilacinus, whereas against A. craccivora cyperotundone, cyperene and cyperene epoxide showed promising toxicity (LD50 = 0.74–0.86 μg/insect). Extract/fractions, EO, and isolated molecules showed a significant reproductive inhibition rate of A. craccivora at higher concentrations. All the tested concentrations of cyperotundone showed significant inhibition of acetylcholinesterase (AChE) and glutathione-S-transferase (GST) in A. craccivora and P. lilacinus. Based upon the present study, C. rotundus can be recommended to control targeted insects in the greenhouse/field conditions after performing bio-efficacy and phytotoxicity studies.

Herbicide potassium glyphosate 660 g/L as weed control in hybrid corn (Zea mays L.) cultivation under no-tillage system ​

Weed management during land preparation is a pivotal concern in agriculture. A viable alternative for weed control in the context of corn cultivation involves employing herbicides within the no-tillage system. Potassium salts formulated with the active ingredient glyphosate allow more effective absorption of glyphosate into the leaves This study aimed to determine the effectiveness and obtain a dose of Potassium Glyphosate 660 g/L herbicide in controlling weeds in corn cultivation with the no-tillage system. The research was conducted from January to May 2023 at the Experimental Garden of the Faculty of Agriculture, University of Padjadjaran, West Java, Indonesia. The experiment used a randomized block design (RBD) with 6 treatments and 4 replications. The treatment consisted of no-tillage with a dose of herbicide with the active ingredient Potassium Glyphosate 660 g/L dose of 1.0 L/ha, 1.5 L/ha, 2.0 L/ha, 2.5 L/ha, 3.0 L/ha, and a control (Perfect Tillage). The application of herbicide with the active ingredient Potassium Glyphosate 660 g/L was carried out once, namely 14 days before corn planting. The experimental results showed that the herbicide with the active ingredient Potassium Glyphosate 660 g/L starting at a dose of 2.0 L/ha – 3.0 L/ha was effective in controlling weeds Imperata cylindrica, Bidens pilosa, Alternanthera sessilis, Ageratum conyzoides, and Borreria alata and other weeds (Mimosa invisa, Cyperus rotundus, Synedrella nodiflora, Axonopus compressus) up to 4 weeks after planting without causing a phytotoxic effect on corn planting and preparation for planting corn and increasing the growth and yield of corn plants.

Two-spotted Spider Mite, Tetranychus urticae Control on Okra by Portulaca oleracea Extract and Cyperus rotundus Oil

The study determined the effect of alcoholic extract of common purslane, Portulaca oleracea, Java grass, and Cyperus rotundus oil in controlling the two-spotted spider mite on okra plants. The results of laboratory experiments showed the efficiency of purslane alcoholic extract was highly toxic to adult mites, but its effect was limited on eggs. In contrast, Cyperus oil was highly effective against mite eggs and adults. Cyperus oil gave the highest mortality of 86.94%, with significant differences from the purslane extract, where 68.61% mortality was observed and differed significantly from the control treatment. An increase in mortality increased with concentration, and the highest mortality of 95.83% was achieved at 5%, compared to the mortality of 59.58% at 1% treatment. The ovicidal activity was significant after the C. rotundus oil treatment compared to the purslane extract application. Cyperus oil reduced egg hatching by 42.50%. However, purslane extract did not affect the hatching significantly (91.70% hatching) and was not significantly different from the control. In general, the hatching rate decreased with increasing concentration of Cyperus oil and was only 40% at the 5% treatment compared to 60 and 96.70% at 1% treatment and controls, respectively.. KEYWORDS :Biological control, Cyperus rotundus, Insecticidal, Ovicidal, Portulaca oleracea, Tetranychus urticae

Allelopathic Potential of Aqueous Plant Extracts Against Seed Germination and Seedling Growth of Weeds

Allelopathy has been described as a negative or positive effect on a specific species of plant caused by a chemical produced by another species of plant. This research analyzed the allelopathic effect of various plant extracts against seed germination and seedling properties of some weed species in two experiments in the autumn of 2021. The impacts of plant extracts, such as peels of pomegranate (Punica granatum), green husk of walnuts (Juglans regia), leaves of eucalyptus (Eucalyptus camaldulensis), galls of Aleppo oak (Quercus infectoria), rhizomes of cogon grass (Imperata cylindrica), and tubers of nutgrass (Cyperus rotundus) plus control on some weed species, like Vaccaria pyramidata, Cynodon dactylon, Sorghum halepense, Trifolium campestre, Portulaca oleracea, Sinapis arvensis, Avena fatua), Hordeum glaucum and Daucus carota were evaluated. The first experiment was conducted in the lab and designed by a factorial-completely randomized design to investigate germination parameters such as final germination percentage (G), mean germination time (MGT), coefficient of the velocity of germination (CVG), and germination rate index (GRI), and another experiment was in a greenhouse and designed in a split-plot pattern, in which the plant extracts were placed in main plots. At the same time, the weed species were distributed in the sub-plots to evaluate the seedling of weeds’ fresh and dry weight and percent of weed inhibition (PWI). The characters’ means (from three replicates) were compared according to Duncan’s multiple range test at the 5% level. The laboratory trial revealed that the green walnut husk was the most successful in reducing all seed germination parameters compared to the control by 13.5, 5.16, and 8.37% for G, MGT, and GRI, respectively. For the greenhouse experiment, the eucalyptus leaves’ extract and pomegranate peels had a higher impact on reducing weed seedlings’ fresh and dry weight and PWI than the control. The most affected species were broad-leaved weeds, while grasses were the least affected. Our results indicated that green walnut husk has a significant role in decreasing seed germination and inhibiting the dry weight of seedlings. Thus, it can be used as a bioherbicide to prevent some weeds from sprouting and growing seedlings.