Purple Flowers Research Articles

Registration of ‘UA 5014C’ Soybean

‘UA 5014C’ soybean [Glycine max (L.) Merr.] (Reg. No. CV‐141, PI 675648) is a high‐yielding early maturity group (MG) V conventional cultivar, developed and released in 2014 by the Arkansas Agricultural Experiment Station. UA 5014C is adapted to Arkansas and other southern states. It was developed by conventional breeding from a cross between two high‐yielding cultivars, ‘Ozark’ and ‘Anand’. The plant population was advanced from F2 to F4 using a modified single‐pod descent method. The F4:5 line was selected and bulk harvested for further agronomic evaluations. Before release, UA 5014C was tested in 125 environments in Arkansas and other southern states from 2006 to 2014 for yield and other agronomic traits, where it had a yield potential comparable to conventional and Liberty Link cultivars of similar maturity. Plants of UA 5014C have determinate growth habit, purple flowers, tawny pubescence, and tan pod walls. Seeds have yellow cotyledons with a dull yellow coat, and black hila. UA 5014C is resistant to southern stem canker [caused by Diaporthe phaseolorum (Cooke & Ellis) Sacc. var. meridionales F.A. Fernandez] and moderately resistant to frogeye leaf spot (caused by Cercospora sojina) and sudden death syndrome [caused by Fusarium solani (Mart.) Sacc. f. sp. glycines].

Transposon Tagging of a Male-Sterility, Female-Sterility Gene, St8, Revealed that the Meiotic MER3 DNA Helicase Activity Is Essential for Fertility in Soybean.

The W4 locus in soybean encodes a dihydroflavonol-4-reductase (DFR2) that regulates pigmentation patterns in flowers and hypocotyl. The mutable w4-m allele that governs variegated flowers has arisen through insertion of a CACTA-type transposable element, Tgm9, in DFR2. In the w4-m line, reversion from variegated to purple flower indicates excision of Tgm9, and its insertion at a new locus. Previously, we have identified a male-sterile, female-sterile mutant among the selfed progenies of a revertant plant carrying only purple flowers. Co-segregation between Tgm9 and the sterility phenotype suggested that the mutant was generated by insertion of Tgm9 at the St8 locus. The transposon was localized to exon 10 of Glyma.16G072300 that shows high identity to the MER3 DNA helicase involved in crossing over. Molecular analysis of fertile branches from two independent revertant plants confirmed precise excision of Tgm9 from the st8 allele, which restored fertility. In soybean, the gene is expressed in flower-buds, trifoliate leaves and stem. Phylogenetic analysis placed St8 in a clade with the Arabidopsis and rice MER3 suggesting that St8 is most likely the orthologous MER3 soybean gene. This study established the utility of Tgm9 in gene identification as well as in forward and reverse genetics studies.

Bioactivity guided isolation of oxypregnane-oligoglycosides (calotroposides) from the root bark of Calotropis gigantea as potent anticancer agents

Bioactivity guided isolation of oxypregnane-oligoglycosides (calotroposides) from the ethanolic extract of root bark ofCalotropis gigantea(L.) Dryand. with purple flowers has been performed and isolated pure compounds has been evaluated for anticancer activity.

Veritas temporis filia est: Truth is the daughter of time

In 1865, Gregor Mendel presented the lectures 'Experiments in Plant Hybridization' concerning his results from cross-breeding experiments with different types of garden pea, performed in his monastery garden in Brno. Mendel studied easily observed pairs of opposite traits, such as purple or white flower, and discovered dominant and recessive traits. He concluded that parents pass separate and distinct factors (today called genes) on to their offspring that are responsible for inherited traits. However, the scientific community did not understand that; indeed it was the beginning of what becomes genetics. The lectures published in 1866, Mendel sent to more than 30 biologists across Europe, but almost no one commented them. In the next 35 years, these papers were only three times cited. The genetics became more important at the beginning of the 20th century, when three different research groups (Hugo de Vries, Carl Erich Correns and Erich von Tschermak with their co-workers) independently re-discovered Mendel's Laws of inheritance. However, as soon as the work was rediscovered, it created controversy. The closeness of Mendel's experimental observations to those predicted by his theories has led to numerous articles and ongoing debate about whether the data could have been obtained in the published form without some falsification. There have been many plausible arguments made for and against this view by a range of eminent geneticists and statisticians. Some have gone so far, as to suggest that the theories ensued from Mendel's two laws were not even correctly formulated in his original paper. The strongest supporters of Mendel's theory became biologist William Bateson and zoologist and geneticist Thomas Hunt Morgan. Morgan argued that genes are located on chromosomes and that the cells chromosomes hold the actual hereditary material, thus created what is now known as classical genetics. For his discovery concerning the role play by the chromosome in heredity, Morgan received the Nobel Prize in 1933. As the architect of genetic experimental and statistical analysis, Mendel remains the acknowledged father of genetics.

Billolivia kyi(Gesneriaceae), a New Species from Vietnam

Billolivia kyi Luu & G. Tran (Gesneriaceae) is described as a new species from the Bidoup-Nui Ba National Park, Vietnam. It is distinct from its closest morphological match B. poilanei in having long petioles, coarsely dentate leaf margins, sparsely pubescent pedicels, ovate calyx lobes not persistent on fruits, and large fruits with persistent connate part of the calyx. Billolivia kyi differs from all other known Billolivia species in having dark red to purple flowers with a yellow patch on the corolla throat, and adaxially sparsely but abaxially densely pubescent laminas.

Elucidation of Molecular Identity of the W3 Locus and Its Implication in Determination of Flower Colors in Soybean.

The wide range of flower colors in soybean is controlled by six independent loci (W1, W2, W3, W4, Wm, and Wp). Among these loci, mutations in the W3 locus under the w4 allelic background (i.e., w3w4) produce near-white flowers, while the W3w4 genotype produces purple throat flowers. Although a gene encoding dihydroflavonol 4-reductase, DFR1, has been known to be closely associated with the W3 locus, its molecular identity has not yet been characterized. In the present study, we aimed to determine whether DFR1 is responsible for allelic variations in the W3 locus. On the basis of the sequence of a DFR probe, Glyma.14G072700 was identified as a candidate gene for DFR1, and nucleotide sequences of Glyma.14G072700 from cultivars with previously validated genotypes for the W3 locus were determined. As a result, a number of nucleotide polymorphisms, mainly single-base substitutions, between both coding and 5′-upstream region sequences of the W3 and w3 alleles were identified. Among them, an indel of 311-bp in the 5′-upstream region was noteworthy, since the Glyma.14G072700 in all the w3 alleles examined contained the indel, whereas that in all the W3 alleles did not; the former was barely expressed, but the latter was well expressed. These results suggest that Glyma.14G072700 is likely to correspond to DFR1 for the W3 locus and that its expression patterns may lead to allelic color phenotypes of W3 and w3 alleles under the w4 allelic background.

DIFFERENTIATION OF THE WHITE AND PURPLE FLOWER FORMS OF Orthosiphon aristatus (Blume) Miq. BY 1D and 2D CORRELATION IR SPECTROSCOPY

Orthosiphon aristatus (Blume) Mig. is also known as Misai kucing or Kumis kucing. The plant is traditionally used to treat tonsilities, fever, menstrual disorders, gonorrhea, syphilis, gallstones, rheumatism, diabetes, hypertension, epilepsy, edema, hepatitis jaundice and for diuretic activity. The objective of this work was to differentiate the white and purple flower forms of O. aristatus (Blume) by multi-steps infrared (IR) macro-fingerprinting. The analyses included Fourier transform infrared spectroscopy (FT-IR), second derivative IR spectroscopy and two-dimensional correlation infrared (2D IR) spectroscopy with thermal perturbation. A distinctive absorption peak at 1384 cm-1 from the purple flower form clearly differentiated the two forms of O. aristatus. Further differentiation was made by the appearance of a strong auto-peak at 1580 cm-1 for the white flower form and at 1546 cm-1 for the purple flower form. Such results indicate that the multi-steps infrared macro-fingerprinting has the potential for the development of a fast and reliable analytical methodology for distinguishing the white and purple flower forms of O. aristatus (Blume) Miq. effectively.

Occurrence and genetic diversity ofRaspberry leaf blotch virus(RLBV) infecting cultivated and wildRubusspecies in Finland

Raspberry leaf blotch virus (RLBV) is a recently described (−)ssRNA virus (genus Emaravirus) associated with the long-known, severe raspberry leaf blotch disorder (RLBD). The virus is presumably transmitted by the raspberry leaf and bud mite (Phyllocoptes gracilis; Eriophyidae). Cultivated and wild raspberries (Rubus idaeus) displaying RLBD or yellowing symptoms were sampled in 14 districts in Finland and tested for RNA3 and RNA5 of RLBV by reverse transcription PCR (RT-PCR) and dot blot hybridization. A total of 59 samples were tested for RLBV, including 36 plants of cultivated raspberry, 20 wild raspberry plants, one ornamental purple flowering raspberry plant (Rubus odoratus), and two samples of P. gracilis (20 mites pooled per sample) collected from RLBD-affected Glen Ample. Fifty-three (93%) of the 57 plants tested were RLBV-positive, including seven raspberry cultivars (Balder, Glen Ample, Jenkka, Malling Minerva, Maurin Makea, Muskoka, Ottawa) and purple flowering raspberry, which is a new host for RLBV. RLBV was also detected by RT-PCR in mites (P. gracilis) collected from buds of RLBV-positive raspberry plants. The partial nucleotide (nt) sequence of the RLBV NP gene (nt 835–1284 of RNA3) was determined for 21 isolates obtained from 10 districts in Finland. Identical isolates were detected in distant districts, and distinctly different isolates were found in the same raspberry cultivation. Furthermore, eight different NP sequence variants were detected in the nine plants of a single raspberry cultivar (Glen Ample) tested from two districts. The nt and deduced amino acid sequences of the 21 isolates were 92.0–100% and 89.3–100% identical, respectively. Phylogenetic analysis revealed two main clusters. One cluster included three isolates, of which two isolates were from one farm in Finland and one isolate was previously characterised in Scotland. The other cluster contained the remaining 19 isolates characterised in this study. The results indicate that RLBV is widely distributed in cultivated and wild raspberries in Finland and shows considerable genetic variability.

Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia.

Anthocyanins are major pigments in plants. Methylation plays a role in the diversity and stability of anthocyanins. However, the contribution of anthocyanin methylation to flower coloration is still unclear. We identified two homologous anthocyanin O-methyltransferase (AOMT) genes from purple-flowered (PsAOMT) and red-flowered (PtAOMT) Paeonia plants, and we performed functional analyses of the two genes in vitro and in vivo. The critical amino acids for AOMT catalytic activity were studied by site-directed mutagenesis. We showed that the recombinant proteins, PsAOMT and PtAOMT, had identical substrate preferences towards anthocyanins. The methylation activity of PsAOMT was 60 times higher than that of PtAOMT in vitro. Interestingly, this vast difference in catalytic activity appeared to result from a single amino acid residue substitution at position 87 (arginine to leucine). There were significant differences between the 35S::PsAOMT transgenic tobacco and control flowers in relation to their chromatic parameters, which further confirmed the function of PsAOMT in vivo. The expression levels of the two homologous AOMT genes were consistent with anthocyanin accumulation in petals. We conclude that AOMTs are responsible for the methylation of cyanidin glycosides in Paeonia plants and play an important role in purple coloration in Paeonia spp.

Tobacco streak virus (strain dahlia) suppresses post-transcriptional gene silencing of flavone synthase II in black dahlia cultivars and causes a drastic flower color change.

Tobacco streak virus suppressed post-transcriptional gene silencing and caused a flower color change in black dahlias, which supported the role of cyanidin-based anthocyanins for black flower appearance. Black flower color of dahlia (Dahlia variabilis) has been attributed, in part, to the high accumulation of cyanidin-based anthocyanins that occurs when flavone synthesis is reduced because of post-transcriptional genesilencing (PTGS) of flavone synthase II (DvFNS). There are also purple-flowering plants that have emerged from a black cultivar 'Kokucho'. We report that the purple color is not caused by a mutation, as previously thought, but by infection with tobacco streak virus (TSVdahlia), which suppresses the PTGS of DvFNS. When TSVdahlia was eliminated from the purple-flowering 'Kokucho' by leaf primordia-free shoot apical meristem culture, the resulting flowers were black. TSVdahlia-infected purple flowers had lower numbers of siRNAs to DvFNS than black flowers, suggesting that TSVdahlia has a silencing suppressor. The graft inoculation of other black cultivars with TSVdahlia altered their flower color drastically except for 'Fidalgo Blacky', a very deep black cultivar with the highest amount of cyanidin-based anthocyanins. The flowers of all six TSVdahlia-infected cultivars accumulated increased amounts of flavones and reduced amounts of cyanidin-based anthocyanins. 'Fidalgo Blacky' remained black despite the change in pigment accumulation, and the amounts of cyanidin-based anthocyanins in its TSVdahlia-infected plants were still higher than those of other cultivars. We propose that black flower color in dahlia is controlled by two different mechanisms that increase the amount of cyanidin-based anthocyanins: DvFNS PTGS-dependent and -independent mechanisms. If both mechanisms occur simultaneously, the flower color will be blacker than if only a single mechanism is active.

Spontaneous mutations of the UDP-glucose:flavonoid 3-O-glucosyltransferase gene confers pale- and dull-colored flowers in the Japanese and common morning glories.

UDP-glucose:flavonoid 3- O -glucosyltransferase is essential for maintaining proper production quantity, acylation, and glucosylation of anthocyanin, and defects cause pale and dull flower pigmentation in morning glories. The Japanese (Ipomoea nil) and the common (I. purpurea) morning glory display bright blue and dark purple flowers, respectively. These flowers contain acylated and glucosylated anthocyanin pigments, and a number of flower color mutants have been isolated in I. nil. Of these, the duskish mutants of I. nil produce pale- and dull-colored flowers. We found that the Duskish gene encodes UDP-glucose:flavonoid 3-O-glucosyltransferase (3GT). The duskish-1 mutation is a frameshift mutation caused by a 4-bp insertion, and duskish-2 is an insertion of a DNA transposon, Tpn10, at 1.3kb upstream of the 3GT start codon. In the duskish-2 mutant, excision of Tpn10 is responsible for restoration of the expression of the 3GT gene. The recombinant 3GT protein displays expected 3GT enzymatic activities to catalyze 3-O-glucosylation of anthocyanidins in vitro. Anthocyanin analysis of a duskish-2 mutant and its germinal revertant showing pale and normal pigmented flowers, respectively, revealed that the mutation caused around 80% reduction of anthocyanin accumulation. We further characterized two I. purpurea mutants showing pale brownish-red flowers, and found that they carry the same frameshift mutation in the 3GT gene. Most of the flower anthocyanins in the mutants were previously found to be anthocyanidin 3-O-glucosides lacking several caffeic acid and glucose moieties that are attached to the anthocyanins in the wild-type plants. These results indicated that 3GT is essential not only for production, but also for proper acylation and glucosylation, of anthocyanin in the morning glories.

Genetic and molecular regulation of flower pigmentation in soybean

Flower color is one of the key traits, which has been widely considered for genetic studies on soybean. A variety of flower colors, such as dark purple, purple, purple blue, purple throat, magenta, pink, near white, and white, has been identified in cultivated soybean (Glycine max). Out of the 19,649 soybean accessions deposited in the United States Department of Agriculture-Germplasm Resources Information Network database, 67 % have purple flowers, 32 % have white flowers, and merely 1 % have flowers with different colors. In contrast, almost all accessions of wild soybean (Glycine soja) have only purple flowers. Flavonoids, mainly anthocyanins, are the most common pigments contributing to flower coloration in soybean. In the recent decades, the flavonoid biosynthesis pathway for anthocyanins has been well established, and some of the genes controlling flower color in soybean have been identified and characterized. Flower pigmentation of soybean is mainly controlled by six independent loci (W1, W2, W3, W4, Wm, and Wp) along with the combination of various other factors such as anthocyanin structure, vacuolar pH, and co-pigments. In this review, we summarize the current status of genetic and molecular regulation of flower pigmentation in cultivated and wild varieties of soybean.

In a world of white, flower colour matters: A white–purple transition signals lack of reward in an alpine Euphrasia

AbstractThe New Zealand alpine flora displays a range of unusual characteristics compared with other alpine floras, in particular the high frequency of species with small white flowers. The presence of both white and bright purple flowers on the same plant in the New Zealand alpine annual creeping eyebright (Euphrasia dyeri Wettst.) provides an ideal opportunity to investigate the significance of flower colour in an environment where coloured flowers are rare. The relationships among flower age, gender phase, reward availability and petal colour were assessed in natural populations of E. dyeri. The effect of pollination on flower colour was tested using hand pollination of bagged flowers. Direct observations and videos of flowers were used to assess patterns of flower visitation by native and introduced pollinators. Unpollinated white E. dyeri flowers changed from white to purple within 6 days. However, pollination of white flowers triggered a significantly faster colour change, typically within 1–2 days. White flowers had receptive stigmas, large amounts of lipid‐rich pollen and small amounts of nectar, whereas stigmas of purple flowers are not receptive and flowers did not provide pollen or nectar rewards. Flowers were mainly visited by native syrphid flies. Both native syrphids and introduced Bombus bees showed a marked avoidance of purple flowers, tending to preferentially visit white flowers. Our study suggests that flower colour change from white to bright purple in E. dyeri functions to direct pollinators to rewarding, receptive flowers. As many Euphrasia L. species are described as having variably coloured flowers, this mechanism may be more widespread in the genus. Furthermore, our results add to the growing evidence that the dominance of white flowers in the New Zealand alpine is not simply due to a lack of colour discrimination among pollinators.

Life-threatening poisoning associated with henbane plant

Hyoscyamus Niger (HN) is a very common and highly hallucinogenic plant which contains anticholinergic agents. The HN is found in every region in Turkey. It is 25-80 cm tall with yellowish, purple flowers. As an annual herbaceous plant, it opens up with a lid-like cover when it is ripe and contains many seeds in its fruits. HN is commonly known as stinking nightshade or black henbane; in Turkey, HN is defined as Ban Plant (Ban Otu) or Crazy Bat (Deli Bat). There are 6 different types of henbane in Turkey. Black henbane and maize henbane are the kinds used in medicine. Maize henbane grows in and around Malatya in Turkey. Its leaves contain hyoscyamine, scopolamine (hyoscine), and several tropane alkaloids. Glycosides is found in the entire body of the plant, though it is dense in the seeds (1). The oral intake can cause central and peripheral anticholinergic effects. It is known that HN poisoning leads to anticholinergic syndromes. In this case report, we are presenting the life-threatening poisoning of a family of four after the accidental oral intake of HN.

Sylvatiins, acetylglucosylated hydrolysable tannins from the petals of Geranium sylvaticum show co-pigment effect

Four hydrolysable tannins, named as sylvatiins A (1), B (2), C (3) and D (4), were isolated from the petals of Geranium sylvaticum. On the basis of spectrometric evidence of NMR analysis (1H NMR, 13C NMR, DQF-COSY, TOCSY, NOESY, HSQC and HMBC), circular dichroism (CD) and ESI-MS/MS, sylvatiins A, B and C were characterized as galloyl glucoses containing one or two acetylglucoses attached to the 3-OH of the galloyl group, whereas sylvatiin D was found to have a chebulinic acid core containing acetylglucose attached in a similar way. The potential of these compounds to act as defensive compounds against herbivores was evaluated using the radial diffusion assay that measures the protein precipitation capacity. In addition, the capacity of sylvatiins to act as co-pigments with anthocyanins of G. sylvaticum petals was measured in vitro at different pH values. Sylvatiins A and D maintained efficiently the purple flower color near the natural pH of petal cells. The amount of sylvatiins was changed according to the flower color; deep purple petals with higher amount of anthocyanin contained more sylvatiins A and C than whiter petals. It was concluded that G. sylvaticum petal cells may accumulate sylvatiins for intermolecular co-pigmentation purposes.

Biosafety and Toxicological Evaluation of Tissue-Cultured Echinacea purpurea Adventitious Roots

Echinacea purpurea (L.) Moench (purple cone flower) is an important medicinal plant; it can enhance immunity, relieve pain, and reduce inflammation, and also has hormonal, antiviral, and antioxidant effects. Adventitious root biomass of Echinacea purpurea was produced in commercial-scale bioreactors for use as a dietary supplement in the food industry and in traditional medicine. Biosafety and toxicological evaluations of tissue-cultured Echinacea purpurea adventitious roots (TCEPARs) were performed. Reverse mutation and chromosomal aberration tests showed no significant mutagenicity. Furthermore, repeated four-week oral dose tests performed in Sprague-Dawley rats did not show any notable changes in the general behavior of the rats, in the gross appearance of their internal organs, or in their mortality rate. There were no differences between the control group and the treatment group in parameters such as absolute body weight, hematology, blood chemistry, and absolute and relative organ weights. These findings indicate that TCEPARs are safe and nontoxic when consumed at an average dietary level and can be used as raw material for traditional medicine and the food industry.

Trading off short-term costs for long-term gains: how do bumblebees decide to learn morphologically complex flowers?

Many animals learn skills that can take a long time to acquire. Such learned skills may have high payoffs eventually, but during the period of learning their net profitability is low. When there are other options available, it is not clear how animals decide to learn how to perform tasks that initially have low or no benefits. Bees in particular visit many types of flowers that vary in the time required to learn how to access their food rewards. We used bumblebees (Bombus impatiens) to address how individuals decide to persevere with learning to handle ‘complex’ flowers. We tested two hypotheses: (1) individuals have unlearned preferences for more complex flowers; (2) individuals use the absolute reward value of the flower to decide whether to learn to handle a particular flower type. We presented individual bees with mixed arrays of colour-distinct ‘simple’ and ‘complex’ flowers, either containing the same value of reward, or where the complex flowers contained twice the concentration of sucrose as the simple flowers. Foragers did not show any unlearned preferences towards the complex flowers, but instead preferred the simple flowers. The strongest initial preferences were for flower colour (purple over pink). Our second hypothesis was supported, because when the purple complex flowers contained a higher reward than the simple flowers, more bees persevered with visiting them, foraging on them exclusively by the end of the test period. There was significant variation between individuals in whether they learned to handle, and how much they visited, complex flowers. These results highlight the complex interplay between unlearned biases and environmental feedback in making decisions about what to learn.

Psoraleaditurnerae and P.vanberkelae (Psoraleeae, Fabaceae): two new species restricted to the Core Cape Region of South Africa.

Two new species of Psoralea L. are described: Psoralea diturnerae A. Bello, C.H. Stirt. & Muasya, sp. nov. and Psoralea vanberkelae C.H. Stirt., A. Bello & Muasya, sp. nov. Psoralea diturnerae is endemic to the Outeniqua mountains (Camferskloof) and is characterised by a mass of numerous basal shoots out of which emerge 2–3 woody stems up to 2 m tall, 3-foliolate needle-like leaflets at the base of the seasonally growing shoot reducing to one towards the apex and bearing numerous 1–3-flowered axillary inflorescences along its length; each mauve to purple and white flower subtended by a trifid cupulum. Psoralea vanberkelae is characterised by its spreading mounding habit, short tightly packed fleshy leaves, with large impressed papillae, densely glandular short broadly triangular stipules, pale to intense mauve to deep blue flowers, standard with a dark purple central blotch above a M-shaped white patch situated above claw, and khaki seeds with purple flecks.

Genetics and Anthocyanin Analysis of Flower Color in Mexican Petunia

The genetics and anthocyanins responsible for flower color were studied in Ruellia simplex Wright (mexican petunia). An F2 population with 153 individuals segregating for four flower colors was developed from a cross between a maternal individual with white corolla with purple throat (WP) and a paternal individual with pink corolla (PK). All the F1 generation had fully purple flowers (P). The F2 generation segregated 94 P:30 PK:24 WP:5 WPK (WPK is a new color combination of white corolla limb and pink throat). These data were separated into groups for corolla limb color and for throat color. The ratio for corolla limb color segregated 94 P:30 PK:29 W, which fits a 9:3:4 recessive epistasis interaction (P = 0.22). The data for corolla throat segregated 118 P:35 PK, which fits a 3:1 ratio (P = 0.54). High-performance liquid chromatography mass spectrometry analyses were performed to elucidate the anthocyanins responsible for the four obtained flower colors. We found that delphinidin derivatives conferred purple corolla color, whereas pelargonidin derivatives were responsible for the pink corolla color. Purple corolla throat color was the result of delphinidin derivatives, whereas the pink color was the result of peonidin derivatives.

Keragaman Karakter Morfologi, Komponen Hasil, dan Hasil Plasma Nutfah Kedelai (Glycine max L.)

Abstract
 Soybean (Glycine max L.) is annual crop that have high morphologies and yield components diversity. The research was conducted at the first season of 2011, the objective of the research were to find morphological, yield, and yield component of Soybean germplasm (Glicine max L.). The research was carried out at experimental station BB-BIOGEN Citayam, Depok, and laboratory of Gene Bank BB-BIOGEN. The experiment used randomized block design with 100 different accessions and three replications for each accession. Based on the observation, the morphological characters have many visual forms. They are as follows: growth percentage in which 19.33 – 99%; growth types were determinate and indeterminate, the leave form was triangle to sharp; purple and white flowers; yellow and black seeds color. The range of values for each characteristic component are as follows: plant height 29,23 – 104,25 cm; number of pods per plant was 23,6 – 99,82; flowering time 33 – 47 days after planting; 100 seed weight 5,98 – 20,77 gram; maturing time 75 – 96,67 days after planting; root nodule’s weight 0,004 – 0,109 gram; seed’s weight 3,15 – 11,45 gram/plant. Among the accessions, the highest yield was shown by B 4323 (643,27 gram/3,6 m2). Significant correlation was shown between soybean’s yield components and yield which were plant’s height, growth percentage, numbers of main stem’s node, numbers of pods, seeds weight for each plant and root nodule’s weight. 100 seeds weight showed significant negative correlation with soybean components.
 
 Key words: germplasm, morphological characteristics, soybean, yield components