Purity Level Research Articles

An innovative approach for the passive cooling of batteries: An empirical investigation of copper deposition on polyurethane foam for the enhancement of phase change material

A proof-of-concept utilising Copper-Plated Polyurethane Foam (CPPF) and Phase Change Material (PCM) for passive thermal management of lithium-ion batteries is demonstrated in this study. The aim of this research is to assess the effectiveness of CPPF when utilised as a constituent substance in PCM/Foam composites. Six distinct configurations of PCM/Foam composites are presented in this work using 10-pore-per-inch foam. A total of four deposition foam samples were produced. Of these, three were created by gradually increasing the immersion time in an electroless copper plating solution. For the fourth sample, an electroless plating technique was utilised for 80 min, followed by an electroplating procedure to deposit an additional layer of copper. The evaluation entails examining each plated sample in comparison to a copper foam that is commercially available with a purity level of 99.99 %. The findings reveal that the electroless-plated specimens exhibit improved effectiveness after being subjected to a prolonged plating period of 80 min. The electroplated sample exhibited the greatest degree of effectiveness, as evidenced by a 64.4 % reduction in battery cell surface temperature(10.98 °C), which is almost identical to the 64.5 % decrease in temperature (11.03 °C) observed with commercial foam but coupled with 88.1 % decrease in mass. The results suggest that the CPPF-PCM composites offer effective passive cooling properties for batteries.

Transcriptome analysis revealed SMURF2 as a prognostic biomarker for oral cancer.

The activation of TGF-β pathway can facilitate tumorigenesis. Understanding the TGF-related genes (TRGs) in oral cancer and determining their prognostic value is of utmost importance. The TRGs were selected to develop a prognostic model based on lasso regression. Oral cancer patients were classified into high-risk and low-risk groups based on the risk model. Subsequently, multivariate COX regression was employed to identify the prognostic marker. Additionally, the expression of SMURF2 was validated using quantitative real-time polymerase chain reaction (qRT-PCR) and the Human Protein Atlas (HPA) database. To investigate the relationship between SMURF2 expression and immune cell infiltrations, we conducted single-sample Gene Set Enrichment Analysis (ssGSEA) analyses. We identified 16 differentially expressed TRGs in oral cancer, all of which showed upregulation. From these, we selected eight TRGs as prognostic signatures. Furthermore, the high-risk group demonstrated lower infiltration levels of immune cells, immune score, and higher tumor purity. Interestingly, we also found that SMURF2 serves as an independent prognostic biomarker. SMURF2 was upregulated in oral cancer, as confirmed by public databases and qRT-PCR analysis. Importantly, our results indicate a close association between SMURF2 expression and the immune microenvironment. The 8-TRG signature prognosis model that we constructed has the ability to predict the survival rate and immune activity of oral cancer patients. SMURF2 could be effective in recognizing prognosis and evaluating immune efficacy for oral cancer.

Determining The Crystallite Size of TiO2/EG-Water XRD Data Using the Scherrer Equation

<p class="Abstract">X-ray diffraction (XRD) data and the Scherrer equation were utilized to analyze the crystallite Size of titanium dioxide (TiO<sub>2</sub>) in a solution of ethylene glycol (EG) and distilled water. The XRD analysis was conducted using a Rigaku Miniflex 600 instrument with an X-ray wavelength of approximately 0.15046 nm. The examination yielded the full-width half maximum (FWHM), which was subsequently examined using the Scherrer equation. This experiment employed TiO<sub>2</sub> with a purity level of 99.8% and a crystal Size of 30 nm. The analysis revealed that the average crystallite Size of TiO<sub>2</sub> in the sample is 19.45 nm, with the highest measurement at about 30.38 nm. The Spearman correlation equation was employed to validate the outcomes. The Spearman's correlation coefficient between the FWHM variable and the crystallite Size of TiO<sub>2</sub> nanoparticles is -0.958. These findings shed light on the crystal structure of TiO<sub>2</sub> under these conditions. These findings lend support to the use of TiO<sub>2</sub> in a variety of nanotechnology applications. However, more research is needed to understand fully how crystallite-Size TiO<sub>2</sub> nanoparticles work in different settings and to find the best ways to prepare samples, including understanding the specific phase and how it affects the stability of fluids. This research contributes significantly to the understanding of the properties of TiO<sub>2</sub> in a solution of distilled water and EG, as well as to the characterization of nanomaterials, with particular emphasis on issue 9 of the SDGS Goal concerning industry, innovation, and infrastructure.</p>

The SRG/eROSITA All-Sky Survey

Clusters of galaxies can be used as powerful probes to study astrophysical processes on large scales, test theories of the growth of structure, and constrain cosmological models. The driving science goal of the SRG/eROSITA All-Sky Survey is to assemble a large sample of X-ray clusters with a well-defined selection function to determine the evolution of the mass function and, hence, the cosmological parameters. We present here a catalog of 12 247 optically confirmed galaxy groups and clusters detected in the 0.2–2.3 keV as extended X-ray sources in a 13 116 deg2 region in the western Galactic half of the sky, which eROSITA surveyed in its first six months of operation. The clusters in the sample span the redshift range 0.003 < z < 1.32. The majority (68%) of these clusters, 8361 sources, represent new discoveries without known counterparts in the literature. The mass range of the sample covers three orders of magnitude from 5 × 1012 Msun to 2 × 1015Msun. We construct a sample for cosmology with a higher purity level (~95%) than the primary sample, comprising 5259 securely detected and confirmed clusters in the 12791 deg2 common footprint of eRASS1 and the DESI Legacy Survey DR10. We characterize the X-ray properties of each cluster, including their flux, luminosity and temperature, the total mass, gas mass, gas mass fraction, and mass proxy YX. These are determined within two apertures, 300 kpc, and the overdensity radius R500, and are calculated by applying a forward modeling approach with a rigorous X-ray background treatment, K-factor, and the Galactic absorption corrections. Population studies utilizing log N-log S, the number of clusters detected above a given flux limit, and the luminosity function show overall agreement with the previous X-ray surveys after accounting for the survey completeness and purity through the selection function. The first eROSITA All-Sky Survey provides an unprecedented sample of galaxy groups and clusters selected in the X-ray band. The eRASS1 cluster catalog demonstrates the excellent performance of eROSITA for extended source detection, consistent with the pre-launch expectations for the final all-sky survey, eRASS:8.

Analysis of the Effect of Crystal form on Precipitated Calcium Carbonate (PCC) of Dead Coral Reefs with the Addition of HNO3 Concentration

Many dead coral reefs found on the coast have not been treated optimally and currently their use is still limited as decoration in aquariums. Coral reefs contain calcium carbonate, silicon dioxide, sodium oxide, magnesium peroxide, and Iron (III) oxide, with a calcium carbonate content of around 73.76%. Because of its high calcium carbonate content, it can be used as a raw material for making PCC (Precipitated Calcium Carbonate) which is widely used in the industrial, health and food sectors. The aim of this research is to obtain the optimal temperature and time for CaO levels, as well as the effect of nitric acid solution concentration on the yield with a high level of purity in PCC products using the carbonization method. The fixed variables are: coral reef mass, independent variables: temperature, calcination time and nitric acid concentration and dependent variables: CaO yield and CaCO3 levels. The calcination temperature used was 700℃, 750℃, 800℃, 850℃, 900℃ and HNO3 concentration 1.50M, 2.00M, 2.50 M, 3.00M, 3.50M with Ca analysis using AAS and PCC morphology and content using SEM-EDX. Optimal conditions are the results of calcination at a temperature of 900℃ with an HNO3 concentration of 3.50 M resulting in a yield of 76% with a purity of 98.58%, pH 8, white with a Ca concentration (ppm) of 1,962,630 and the crystal form obtained at 3.00 M HNO3 is calcite and 3.50 M HNO3 is vaterite.

A facile approach for regeneration of graphite anodes from spent lithium-ion battery

Recycling of lithium-ion battery anodes has become a topic of interest due to the retirement of batteries. However, existing recycling methods have either been environmentally unfriendly or lacked high purity levels. Here, a simple and low-cost recycling method is developed. Firstly, organic matter in graphite is eliminated through heat treatment. The removal of metal impurities in graphite is then carried out by utilizing sulfuric acid (H2SO4) leaching. Lastly, potassium hydroxide (KOH) etching is utilized to restore the structural defects of graphite. This comprehensive process enhances the cycling stability and rate capability of graphite. The initial discharge capacity at 0.1 C can reach a specific capacity of 422.42 mAh/g, and the specific capacity of the discharge after 100 cycles is 365.97 mAh/g, which reaches the standard of commercial graphite (CG). The results demonstrate that the method effectively purifies and regenerates waste graphite (WG). This process can alleviate resource pressure while being suitable for industrial applications due to its simplicity and low cost.

Effect of the boson peak and the ionic resonance in the dielectric properties of silicate materials at mm-wave and THz frequencies

In this paper, a broadband measurement of the dielectric properties of silicates has been done to cover the seldom characterized mm-wave spectrum and the low THz spectrum. The dielectric properties, i.e. loss and permittivity, show a frequency independent response up to approximately 40 GHz and the loss monotonically increases to the THz frequency range. Two resonance peaks appear at THz frequencies: the first is the boson peak, and the second one corresponds to the ionic resonance of the network former, which in this case is silicon. Even though the materials belong to different groups in the silicate family (crystalline and non-crystalline, with different levels of purity), they seem to have the same overall tendency in the dielectric properties. Argand plots are used for the first time at THz frequencies to provide physical insight into the boson peak and ionic resonance. The complex plane analysis reveals details on the polarization mechanisms underlying silicate network vibrations through direct visualization of their characteristic signatures, and simplifies the process of modeling the dielectric response, leading to a better fit.

Performance assessment of green hydrogen generation via distinct electrolytes dedicated to renewable energy

Water electrolysis is the most established and effective method for generating green hydrogen. This research aim is to investigate the electrolyzer system model, by integrating several electrolytes (i.e., NaOH, KOH and H2SO4) and, the mathematical framework which depends on the foundation of thermal, electrochemical and system analysis. The system in Aspen Plus, with a maximum electrolyzer power of 15 kW, has been modelled by using the experimental reference system. This model relies on empirical expressions that explain how the cell potential, Faraday efficiency, excess heat, net power, and gas purity alters, at an operational conditions like temperature and pressure as well as the current density. The outcome demonstrates that at 10 bar pressure, electrolyte produces hydrogen with 99.84 % purity level. The maximum system efficiency of 84.13 % is recorded, whereas the maximum hydrogen production of 7.994 Nm3/h by using NaOH which is 4.57 % and 12.23 % higher than KOH and H2SO4 electrolytes when current density is 0.55 A/cm2. The economic analysis is done by evaluating maximum capital cost of 3042.78 k$/year. Also, sensitivity analysis has been done to evaluate the performance of electrolyzer ability for splitting of water into hydrogen and oxygen.

Highly efficient recovery of vanadium from Abu Zeneima ferruginous siltstone, Southwestern Sinai, Egypt, by a novel polyimine‐based chelating ligand

AbstractTo extract V5+ ions from the ferruginous siltstone in the Abu Zeneima region of Southwestern Sinai, Egypt, a chelating polyimine ligand (PVAm‐Ben) was synthesized via a condensation reaction involving polyvinyl amine and α‐hydroxy ketone (Benzoin). The structure of PVAm‐Ben underwent comprehensive characterization employing various techniques, namely; FT‐IR, 1H‐NMR, 13C‐NMR, and GC/MS analyses. Experimental factors controlling such as pH, time, initial concentration, dosage, temperature, and stripping, were systematically optimized to enhance measurement accuracy. Under conditions of 25°C, 0.015 mol/L, and 30 minutes, PVAm‐Ben/C2H4Cl2 exhibited remarkable retention capacity at pH 3. Linear regression analysis results suggested the release of a mole of H+ during the extraction process. The stability constant (log β = 2.1882) of the PVAm‐Ben/V5+ complex was determined, yielding a value of 154.24. The stoichiometry mechanism between PVAm‐Ben and V5+ ions displayed a linear correlation (slope: 0.9922), signifying 1 mole of PVAm‐Ben reacting with 1 mole of vanadium. The pseudo‐second‐order kinetic model indicated an exothermic, spontaneous, and advantageous extraction process at low temperatures. Efficient stripping of PVAm‐Ben/C2H4Cl2 loaded with vanadium was achieved using 0.05 M NaOH with a 99% efficiency rate. Ultimately, enhanced variables were employed to yield a V2O5 concentrate with a vanadium content of 55% and a purity level of 98.21%.

Ethanol-Based Extraction of Annatto (Bixa Orellana L.) and Characterization of the Bixin and Norbixin.

Bixa orellana dye was extracted from the seeds by the method of Soxhlet extraction using ethanol where a percentage yield of 31.4 was obtained. Recrystallization of the dye was performed using chloroform and ethanol to increase the degree of purity. Thin layer chromatography was used to separate the dye into Bixin and Norbixin with Rf values of 0.56 and 0.42, respectively. The extracted dye was pH sensitive, insoluble in water but soluble in some organic solvents like ethanol and acetic acid. Characterization by UV-vis spectroscopy showed maximum absorption for Bixin and Norbixin at 457 and 453 nm, respectively. Fourier transform infrared spectra of Bixin and Norbixin showed bands confirming similar functional groups for both Bixin and Norbixin except for -CO- stretching and methylene vibration which was absent in Norbixin. With the yield, purity level, and other properties of the extracted dye, the comparative advantages of ethanol-based Soxhlet extraction of the dye were well established.

Ectoine production from a novel bacterial strain and high-purity purification with a cost-effective and single-step method

This study marks the exploration into the production of ectoine, a valuable compound with significant potential as an antioxidant, osmoprotectant, anti-inflammatory agent, and stabilizer of cell membranes, proteins, and DNA integrity. Our focus centred on investigating the presence of ectoine and optimizing its production by the novel ectoine producer bacterial strain, Piscibacillus halophilus. For the optimization of ectoine production the effects of carbon and nitrogen sources, salt, pH, agitation and incubation period were optimized by one-factor-at-a-time. We started with an initial ectoine content of 46.92 mg/L, and through a series of optimization processes, we achieved a remarkable increase, resulting in an ectoine content of 1498.2 mg/L. The bacterial species P. halophilus achieved its highest ectoine production after 48 h of incubation, with conditions set at 10 % (w/v) salinity, pH of 7.50, and an agitation speed of 160 rpm. These precise conditions were found to be the most favourable for maximizing ectoine production by this strain. Besides, we have achieved successful purification of ectoine from the crude extract through a streamlined single-step process. This purification method has delivered an exceptional level of purity, surpassing 99.15 %, and an impressive yield of over 99 %. Importantly, we accomplished this using readily available and cost-effective strong acids (HCl) and strong bases (NaOH) to arrange pH gradients. The use of acid and base in the purification process of ectoine reflects an innovative and sustainable methodology.

Targeted phasing of 2–200 kilobase DNA fragments with a short-read sequencer and a single-tube linked-read library method

In the human genome, heterozygous sites refer to genomic positions with a different allele or nucleotide variant on the maternal and paternal chromosomes. Resolving these allelic differences by chromosomal copy, also known as phasing, is achievable on a short-read sequencer when using a library preparation method that captures long-range genomic information. TELL-Seq is a library preparation that captures long-range genomic information with the aid of molecular identifiers (barcodes). The same barcode is used to tag the reads derived from the same long DNA fragment within a range of up to 200 kilobases (kb), generating linked-reads. This strategy can be used to phase an entire genome. Here, we introduce a TELL-Seq protocol developed for targeted applications, enabling the phasing of enriched loci of varying sizes, purity levels, and heterozygosity. To validate this protocol, we phased 2–200 kb loci enriched with different methods: CRISPR/Cas9-mediated excision coupled with pulse-field electrophoresis for the longest fragments, CRISPR/Cas9-mediated protection from exonuclease digestion for mid-size fragments, and long PCR for the shortest fragments. All selected loci have known clinical relevance: BRCA1, BRCA2, MLH1, MSH2, MSH6, APC, PMS2, SCN5A-SCN10A, and PKI3CA. Collectively, the analyses show that TELL-Seq can accurately phase 2–200 kb targets using a short-read sequencer.

Formulation and Synthesis of Vanillin from Clove Oil as a Chemosensor for Urea Detection in Urine

Urea levels in urine play an important parameter in diagnosing bodily conditions through liver and kidney examinations. The typical reagent for detecting urea content in urine is para-dimethylaminobenzaldehyde (pDMAB). However, it has a drawback related to the instability of color in the resulting Schiff base compound (pDMAB-urea). In this study, the synthesized vanillin compound derived from clove oil serves as the foundational material for a urea chemosensor based on the colorimetric concept. The synthesized vanillin was characterized using FTIR, GC-MS, and 1H-NMR. The formulation of the vanillin compound as a sensor was conducted by assessing the suitable solvent, determining the optimal mass of vanillin, and evaluating the acid-base conditions of the sensor formulation system both qualitatively and quantitatively. Subsequently, the most effective formulation was selected for detecting urea in urine samples. The synthesis of vanillin yielded a purity level of 95%. The optimal formulation was obtained at an optimum mass of vanilla of 0.75 g in 50 mL of 96% ethanol and 10 mL of 10% NaOH. The color change in the sample was from colorless to greenish yellow (436 nm). The vanillin obtained was applied to urine samples with the best results at a sample dilution level of 10,000×.

Simultaneous Separation of Protactinium-230 and Uranium-230 Isotopes from a Proton-Irradiated Thorium Matrix.

The use of radioisotopes in cancer therapy is becoming increasingly important. As a potential candidate for targeted alpha therapy, 230U (t1/2 = 20.8 d), the decay daughter of 230Pa (t1/2 = 17.4 d) is currently being investigated for cancer treatment. For radioisotopes to be used in biomedicine, they must be radiochemically pure and free from carrier interference. This can be challenging given their short half-life. Thus, radiological separation methods for harvesting isotopes for use in biomedicine must be simple, fast, and capable of obtaining the required purity levels. Herein, we demonstrate a new rapid method for the separation of 230U and 230Pa from a proton-irradiated 232Th metal target and from coproduced fission products with high recovery and purity. A dual-column approach was used, in which the first column was an anion exchange (AX) column and the second column was a DGA column. The MP-1 AX resin was used for the primary separation of the three major components (U, Pa, and Th, as well as fission products), and the DGA column was used for further purification of the separated 230U. The method is simple and allows for straightforward separation of U, Pa, Th, and fission products; also, the entire process from target dissolution to shipment of 230U could be completed in 3 days with an overall 230U recovery yield of 96 ± 3%. Additionally, separated 230Pa fractions can be used to harvest ingrown 230U after initial separation.

Application of the Support Vector Regression Method with the Grid Search Algorithm to Predict Movement Gold Price

Gold is an investment with the smallest risk because it can be sold anytime and anywhere. In Indonesia, gold bullion as an investment product is known for its purity level of 99.99%, namely gold bullion produced by PT. Aneka Tambang (Antam) through its Precious Metals business unit. Apart from its pure production, Antam gold bullion is easier to resell anytime and anywhere because it has an official certificate from the international gold standardisation institution, namely LBMA (London Bullion Market Association), to more easily estimate the value of gold bullion when sold. To overcome this, predictions of future gold prices are needed. In this research, one of the prediction methods is Support Vector Regression with the Grid Search Algorithm. In this method this method will be used to predict the price of gold, which aims to predict and find out the price of gold one year in the future to produce a level accuracy (MAPE) of 5.43% and the prediction of gold prices increasing from 2023-June-01 to 2024-March-23 while experiencing a decline starting in 2024-March-24. Research by examining the relationship between variables, which emphasises data consisting of numbers so that it is analysed based on statistical procedures using the Support Vector Regression method with data sourced from the daily price of gold bullion through PT. Gallery 24 Pawnshops, North Sumatra. Where this method is very well used in predicting by choosing the best kernel used is the linear kernel because, from these three kernels, the best hyperparameters were obtained for predicting gold price movements using a linear kernel with a division for training and testing data of 60: 40. The MAPE value obtained was 5.43.

The Roles of Impurities and Surface Area on Thermal Stability and Oxidation Resistance of BN Nanoplatelets.

This study considers the influence of purity and surface area on the thermal and oxidation properties of hexagonal boron nitride (h-BN) nanoplatelets, which represent crucial factors in high-temperature oxidizing environments. Three h-BN nanoplatelet-based materials, synthesized with different purity levels and surface areas (~3, ~56, and ~140 m2/g), were compared, including a commercial BN reference. All materials were systematically analyzed by various characterization techniques, including gas pycnometry, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared radiation, X-ray photoelectron spectroscopy, gas sorption analysis, and thermal gravimetric analysis coupled with differential scanning calorimetry. Results indicated that the thermal stability and oxidation resistance of the synthesized materials were improved by up to ~13.5% (or by 120 °C) with an increase in purity. Furthermore, the reference material with its high purity and low surface area (~4 m2/g) showed superior performance, which was attributed to the minimized reactive sites for oxygen diffusion due to lower surface area availability and fewer possible defects, highlighting the critical roles of both sample purity and accessible surface area in h-BN thermo-oxidative stability. These findings highlight the importance of focusing on purity and surface area control in developing BN-based nanomaterials, offering a path to enhance their performance in extreme thermal and oxidative conditions.

Advancing Manufacturing Efficiency through Real-time Production Monitoring and Control Systems

The advantages of both real-time production monitoring and control systems in enhancing the advancement of manufacturing efficiency cannot be overlooked. Manufacturing industries need to meet customers’ demand while still maximizing their profits via avoiding emergency shutdown of plants during production. Nonetheless, quality products with high purity level should be supplied uninterruptedly without breakage of supply chain. In recent times, failure of many of manufacturing industries to deliver their products to end users have been cited and reported. This was due to inadequate scheduling which was not being monitored via real-time production and control systems. There is need to tackle this problem which calls for the adoption of real-time production monitoring and control systems. In this paper, the concepts behind the use of real-time production monitoring and control systems were discussed. Consideration was given to how real-time production monitoring can be integrated in manufacturing. The features of real-time control systems were discussed alongside with their industrial applications in various disciplines of Engineering. Data analysis and prognostics, data collection, visualization module and data storage were identified as the relevant sequential steps in real time production monitoring in advancing manufacturing efficiency. In conclusion, the significance and importance of real-time production monitoring and control systems in advancing manufacturing efficiency have been revealed and discussed in this paper.

High-purity rayon-based carbon fiber felt prepared by halogen gas purification for superior thermal insulation and oxidation resistance

High-purity rayon-based carbon fiber (CFRay) felt holds significant promise for applications in thermal protection systems and semiconductor crystal growth furnaces. This study employs halogen gas purification and high-temperature purification techniques in the fabrication of high-purity CFRay felt. Additionally, the influence of the purification process on the thermal insulation and oxidation resistance of CFRay felt is investigated. The glow discharge mass spectrometer analysis reveals that purification at a lower temperature of 2300 °C achieves a purity level of 6.9 ppm, surpassing both purified CFRay felt without halogen at 2300 °C (56.1 ppm) and high-temperature treated CFRay felt at 3000 °C (10.4 ppm). Thermal insulation and dynamic-static oxidation resistance tests show that the properties of CFRay felt are concurrently affected by the heat treatment temperature. Compared to high-temperature purification, halogen gas purification operates at lower temperatures, preventing the graphitization of CFRay, thereby ensuring high purity while enhancing thermal insulation and demonstrating relatively superior oxidation resistance. This study offers novel insights into the preparation and performance adjustment of high-purity CFRay felt.

Fibroblast activation protein promotes progression of hepatocellular carcinoma via regulating the immunity.

Fibroblast activation protein (FAP) has been indicated to express in cancer-associated fibroblasts (CAFs) in most cancers. This work was dedicated to exploring FAP's effects on hepatocellular carcinoma(HCC). The data were extracted from The Cancer Genome Atlas, Gene Expression Omnibus, ImmPort, and Reactome databases. The correlation between FAP and HCC patients' prognosis was explored via survival analysis. The qRT-PCR and western blot analysis were used to analyze the FAP mRNA and protein expression levels, respectively. The cell proliferation and apoptosis were determined using the cell counting kit-8 assay kit and Annexin V-FITC/PI apoptosis kit, respectively. The HCC patients with FAP overexpression displayed a worse prognosis. The FAP expression was positively associated with the infiltration levels of tumor purity, B cell, CD8 + T cell, CD4 + T cell, macrophage, neutrophil, and dendritic cell. The optimal nine immune related genes were screened between two groups (FAP high vs. low). Moreover, we identified 24 energy metabolism related genes (FAP high vs. low) and these 24 genes were highly expressed in the high FAP expression group. The FAP expression had a significant positive correlation with the expression of PD-1, CTLA4, PDL-1, and PDL-2. The FAP overexpression promoted proliferation and migration while inhibiting the apoptosis of HCC cells. The FAP overexpression promoted the progression of HCC by regulating the immunity to affect the prognosis of HCC patients, thereby serving as a poor prognostic marker for HCC patients.

Purification of Anti-Mycobacterium tuberculosis MPT64 Immunoglobulin-Y from Egg-Yolk Supernatant Using Thiophilic Adsorption Chromatography

The significance of immunoglobulin Y (IgY) as a particular antibody equal to mammalian IgG is well understood. However, due to a lack of reliable purification procedures, producing highly pure IgY remains problematic. In this study, we aimed to optimize the recovery of pure IgY anti-MPT64 using thiophilic adsorption chromatography. The purification of IgY anti-MPT64 was achieved by initial PEG lipid precipitation, then an optimized purification by varying the gradient concentration of elution buffer into five steps gradient (0-20, 20-40, 40-60, 60-80, and 80-100%v/v) for three injection column volume (CV) each and two steps gradient (0-50 and 50-100%v/v) in eight CV for each concentration. The obtained IgY was characterized by SDS-PAGE and dot-blot then determined its content levels using the Lowry method. The results showed that the five steps gradient purification was found to provide a better purity level of IgY than the two steps gradient. However, the IgY content obtained in the two steps gradient purification (2.2632± 0.011 mg/mL) was higher than the five steps gradient purification (1.35482 ± 0.023 mg/mL). Nevertheless, both purified IgY results can recognize MPT64 protein through a dot blot test. Therefore, it can be summarized that thiophilic adsorption chromatography with five steps gradient of purification was an efficient process to obtain a higher purity of IgY anti-MPT64, especially to be targeted as a diagnostic kit component for MPT64 detection.