Product Purity Research Articles

Lemon Juice as a Natural Biodegradable Catalyst for an Ecofriendly One-Pot Pseudo-Three-Component Synthesis of N,1-Dimethyl-6-(methylsulfanyl)-3,5-dinitro-1,4-dihydropyridine-2-amines

AbstractA one-pot pseudo-three-component reaction strategy has been designed for synthesizing all-carbon-functionalized hexasubstituted N,1-dimethyl-6-(methylsulfanyl)-3,5-dinitro-1,4-dihydropyridine-2-amines under neat conditions using inexpensive, readily available, natural, biodegradable lemon juice as a catalyst. The acidic reaction environment induced by lemon juice provided a superior catalytic performance, with a high reaction rate and high product yields, to those of a pool of Lewis and Brønsted acid catalysts. The wide substrate scope and functional-group tolerance permitted diversity creation with electronic and structural variations in a robust and operationally simple manner. The recoverability/reusability of the lemon juice catalyst and the easy purification of the end products by precipitation/filtration avoid the need for column chromatography or hazardous organic solvents and confirm that this is an environmentally benign sustainable technology. Moreover, a gram-scale operation and chemical modifications of the final products demonstrated some promising advantages.

Direct TiH2 powder production by the reduction of TiO2 using Mg in Ar and H2 mixed gas atmosphere

To develop a direct production process for TiH2 powder from TiO2, the reduction of TiO2 using Mg in molten MgCl2 – KCl salt under a high hydrogen chemical potential was investigated. The reduction of nano-sized TiO2 powder was conducted at 973 – 1073 K under an Ar or Ar and 10% H2 mixed gas atmosphere when the mass ratios of Mg to feed and salt to feed were 1.14 – 2.86 and 0.87 – 3.48, respectively. The results showed that the oxygen concentration in the Ti product decreased as the mass ratio of salt to feed and temperature decreased. Furthermore, according to the variation in Mg amounts, the oxygen concentration was 0.350 – 0.441 mass%. In addition, employing hydrogen during the reduction enhances the capability to decrease the oxygen concentration in the Ti product. Moreover, the hydrogen concentration in the Ti product increased as the amount of molten salt decreased, thereby enabling pure TiH2 production. As a result, TiH2 powder with an oxygen concentration of 0.350 mass% was obtained under a certain condition. These results demonstrate the feasibility of the direct production of low-oxygen TiH2 powder from TiO2 via reduction at 973 K using Mg in an Ar and H2 mixed gas atmosphere.

Empowering Community Entrepreneurship Through Salt-Based Mouthwash Production

In this PKM program, our partner is residents in Sedayulawas, Lamongan, East Java, Province. This community has generated the production mouthwash derived from salt. Salt is a mineral composed primarily of sodium chloride (NaCl). Diversification of salt products with various derivative products is very possible for salt farmers to do as an effort to empower the economy of the Lamongan community as well as increase food security through the provision of quality salt and downstream technology to produce salt derivatives that have more value for example mouthwash. Mouthwash containing saltwater rinses work by increasing the pH- balance inside the mouth, creating a much more alkaline oral environment in which the bacteria are no longer able to thrive. Based on interviews and observations in the field, there are 3 main focuses of the problems currently faced by partners, namely: 1) lack of knowledge about downstreaming of salt products and their derivatives and 2) the packaging of product. Efforts to increase salt production and salt purification technology as well as alternative diversification of salt derivative products can be a model that can drive the economic sector of the community of salt farmers and women who boil salt so as to initiate the opening of new job opportunities and create superior salt products and derivative products, namely mouthwash in Lamongan Regency. This activity was realized with an approach in the form of making a sustainable cooperation program until the end of PKM, creating a family atmosphere between the two and understanding that the problems experienced were a shared problem so that they could be solved together according to the level of responsibility to achieve the expected benefits, namely increased yields, production and productivity and competitiveness, independence and welfare of the community.

The influence of feed molar flow rate on key streams properties and duties in ethanol-water azeotropic distillation process

Investigation on the influence of near azeotropic feed molar flow rate on properties of key streams connecting main process units of an ethanol-water azeotropic distillation pilot processis presented. The heterogeneous azeotropic distillation model with cyclohexane entrainer was configured in Aspen Plus® V10 commercial simulation software. Property analysis and prediction of plant’s performancewere achieved using Non-Random Two Liquid Redlich-Kwong thermodynamic model. Both residual curve maps and ternary diagrams were used to determineseparation possibility and presence of ethanol-water azeotrope in the formed ternary mixtureduring distillation synthesis. The process convergence was addressed using flowsheet convergence and balance node. Distillation of ethanol- water azeotropic mixture was simulated at constant recycle ratio (R = 5), numberof stages (N = 12) and pressure (P = 1 atm). Data obtained were analysed in MS Excel 2013. Results showed that simultaneous increase ofmolar flow rate of the near azeotropic feed and feed ethanol concentration improves ethanol purity but results in retrograde phenomenon which may humper plant’s performance and increase stream flow rates and therefore increase energy duties. The condenser duty of the main column wasaround 90.94% higher than the recycle column’scondenser duty. Also, reboiler duty of the main column were nearly 90.25% higher than the recycle column’s reboiler duty.It was concluded that setting 18 to 20 kmol/h feed molar flow rate enhances higher energy efficiency and improves ethanol product purity. Careful distillation synthesis and plant’s monitoring are recommended to improve ethanol-water azeotropic distillation plant’s performance and address retrograde phenomena.

Bioprocess development for microbial production and purification of cellobiose lipids by the smut fungus Ustilago maydis DSM 4500.

Cellobiose lipids (CBLs) are a class of glycolipid biosurfactants produced by various fungal strains. These compounds have gained significant interest due to their surface-active and antifungal properties, which are comparable to traditional synthetic surfactants and antimicrobials. Despite their potential applicability in various cosmetic, pharmaceutical, and agricultural formulations, significantly less research has been focused on their production and purification in comparison to other glycolipid biosurfactants, such as mannosylerythritol lipids (MELs) and sophorolipids. Hence, this work proposes the development of a bioprocess that involves the microbial production and high-level chromatographic purification of CBLs from a submerged culture of Ustilago maydis DSM 4500. After a highly purified CBL product was obtained, the factors affecting the production of this glycolipid were investigated. It was demonstrated that U. maydis DSM 4500 produces a specific structural variant of CBLs at a concentration of 1.36g/L on an optimized the growth medium. Also, it was established that when the C/N ratio was decreased, the CBL titer increased by 2.3-fold. Furthermore, supplementing the culture with ZnSO4 at a concentration of 0.04mg/L further increased CBL concentration to 4.95g/L, representing the highest CBL titer achieved in a single-stage bioprocess to date. This study developed a methodology for utilizing U. maydis as a high-level CBL producer, which could challenge other familiar CBL producers, such as Sporisorium scitamineum and Cryptococcus humicola.

Immobilization of Glycosyltransferase into a Hydrophilic Metal-organic Framework for Efficient Biosynthesis of Chondroitin Sulfate.

Chondroitin sulfate (CS) is a structurally complex anionic polysaccharide widely used in medical, cosmetic and food applications. Enzymatic catalysis is an important strategy for synthesizing CS with uniform chain lengths and well-defined structures. However, the industrial application of glycosyltransferases is hindered by limitations such as low expression yields, poor stability, and challenges in reuse. We developed a mild and rapid one-step synthetic method for the efficient immobilization of chondroitin synthase (KfoC). The resulting KfoC@ZIF-90 composite exhibits high catalytic activity, thermal stability, and pH adaptability. Notably, KfoC@ZIF-90 exhibited 5-fold enhanced thermal stability at 40°C and retained 86% relative activity at pH 10, while also maintaining 90% activity in organic solvents, surpassing the performance of free KfoC. Molecular docking analysis revealed that encapsulating KfoC within hydrophilic ZIF-90 reduced its binding energy to the substrate, thereby improving catalytic performance. Furthermore, KfoC@ZIF-90 can be easily separated from the reaction solution by centrifugation, simplifying product isolation and purification while enabling enzyme reuse. These attributes significantly enhance operability and reduce processing costs, making enzymatic CS synthesis more feasible for industrial applications.

Norditerpene natural products from subterranean fungi with anti-parasitic activity.

Cryptosporidium is a common, waterborne gastrointestinal parasite that causes diarrheal disease worldwide. Currently there are no effective therapeutics to treat cryptosporidiosis in at-risk populations. Since natural products are a known source of anti-parasitic compounds, we screened a library of extracts and pure natural product compounds isolated from bacteria and fungi collected from subterranean environments for activity against Cryptosporidium parvum . Eight structurally related norditerpene lactones isolated from the fungus Oidiodendron truncatum collected from the Soudan Iron mine in Minnesota showed potent activity and were further tested to identify the most active compounds. The availability of a diverse suite of natural structural analogs with varying activities allowed us to determine some structure activity relationships for both anti-parasitic activity as well as cytotoxicity. The two most potent compounds, oidiolactones A and B, had EC 50 s against intracellular Cryptosporidium parvum of 530 and 240 nM respectively without cytotoxicity to confluent HCT-8 host cells. Both compounds also inhibited the related parasite Toxoplasma gondii . Oidiolactone A was active against asexual, but not sexual, stages of C. parvum , and killed 80% of the parasites within 8 hours of treatment. This compound reduced C. parvum infection by 70% in IFNγ-/- mice, with no signs of toxicity. The high potency, low cytotoxicity, and in vivo activity combined with high production, easy isolation from fungi, and synthetic accessibility make oidiolactones A and B attractive scaffolds for the development of new anti- Cryptosporidium therapeutics.

Efficient Alkaline-Free Electrooxidation of 5-Hydroxymethylfurfural to 2,5-Furandicarboxylic Acid using Electrochemically-Charged NixCo1-x(OH)2 as a Redox Mediator.

Converting biomass-derived molecules like 5-hydroxymethylfurfural (HMF) into value-added products alongside hydrogen production using renewable energy offers significant opportunities for sustainable chemical and energy production. Yet, HMF electrooxidation requires strong alkaline conditions and membranes for efficient conversion. These harsh conditions destabilize HMF, leading to humin formation and reduced product purity, meanwhile membranes increase costs. Addressing these challenges, we introduce a two-step, decoupling system that operates without strong alkaline conditions and eliminates the need of membranes. In this system, nickel-cobalt hydroxides serve as effective redox mediators, driving HMF oxidation in pure water. The experimental results showed that Ni0.85Co0.15OOH effectively promotes the dehydrogenation of substrate and achieves a highly efficient oxidation of HMF in pure water, with the selectivity of product 2,5-furandicarboxylic acid (FDCA) approaching 100 %. This system has been expanded to oxidize various substrates, achieving yields exceeding 92 % for the corresponding acids of functionalized compounds such as furfuryl alcohol, furfural, benzyl alcohol, and benzaldehyde. By replacing fixed electrodes with flow electrodes, the scalability of decoupling strategy was evaluated for the harvesting of pure solid FDCA, highlighting the broad prospects in practical applications.

Influence of hydrophobic chain structure and headgroup ionization of amino acid surfactants on emulsion stability and recyclable liquid-liquid interfacial catalysis

Emulsion catalysis, a subset of ’on-water’ catalysis, is influenced by surfactants that act as emulsion stabilizers. The hydrophobicity of these surfactants, closely tied to the molecular structure of their chains and their headgroup ionization, plays a crucial role in controlling emulsion reactions and subsequent processes of surfactant recovery and product purification. However, the influence of the specific chain structure of surfactants and its cooperation with the headgroup in different solution environments on the emulsion reaction and demulsification processes remains uncertain. In this study, the stability of emulsions stabilized by amino acid surfactants featuring various hydrophobic chains, specifically side chains, was studied across a range of pH conditions. Amino acid surfactants with long chain are pivotal in enhancing the stability of emulsions. The combined effect of hydrogen bonding and electrostatic interactions between the amino acid headgroups boosts the stability when the pH of the solution aligns closely with the pKa2 of the surfactants. Under these conditions, a relatively stable oil–water interface significantly increases the yield of the Knoevenagel reaction to 94 %. As the pH of the system increases, the surfactants become more hydrophilic, enabling the phase separation of the emulsion, which facilitates the collection of the product and the recycling of the surfactants. This study provides important insights on regulating the structure and function of the oil–water interface based on surfactants and proposes a potentially effective approach for environmental-friendly and convenient chemical synthesis.

Continuous flow synthesis of N,N-dimethyltryptamine (DMT) analogues with therapeutic potential.

Herein, we describe the continuous flow synthesis and in-line extraction of N,N-dimethyltryptamine (DMT) and several of its analogues using a Fischer indole reaction, along with a larger gram scale synthesis (4.75 g) of the model compound. These products could then be quickly transformed into their respective fumarate salts, making them easier to handle and stable for long time storage using a straightforward batch procedure. Additionally, the commercially available drug rizatriptan benzoate could be synthesised with high purity using this setup. The presented method employs relatively green solvents both for the synthesis and purification of the target products.

Prolonged self-assembly of H. pylori ferritin globules at physiological conditions.

One of the promising drug delivery tools is ferritin, which features high stability at a wide range of conditions and protects cargo by its spherical protein shell. We studied the self-assembly into homoglobules of ferritin from H. pylori and a chimeric protein ferritin-SUMO. We exposed the globules to pH-driven dis/reassembly and in both cases we observed two fractions during size exclusion chromatography (SEC) procedure. The higher molecular weight fraction contained fully assembled globules of ferritin and ferritin-SUMO that well coincides with literature. Interestingly, the lower molecular weight fraction contained intermediate subglobular oligomers that also formed globules, but on a time scale of hours, while being under physiological conditions. We performed biochemical characterization of this fraction and found that, in the case of ferritin, it contained almost the whole range of intermediate oligomers with different stoichiometry. In contrast, the ferritin-SUMO fraction contained only two distinct states: dimers and globules, without any other ferritin-SUMO intermediate oligomers. We built AlphaFold-derived schemes of ferritin and ferritin-SUMO self-assembly which also indicated differences in their assembly pathways. Our results could potentially open the possibility of cargo loading into ferritins at physiological conditions and improved purification of ferritin-based products if using a ferritin-SUMO modification with following cleavage of the SUMO-tag by the SUMO protease.

In-situ immobilization of a novel fixed Z-scheme Ag|Ag2S/Ag/SnO2 photocatalytic system for pure hydrogen production and synchronous tartrazine degradation: Efficiency and mechanism insight

In-situ immobilization of a novel fixed Z-scheme Ag|Ag2S/Ag/SnO2 photocatalytic system for pure hydrogen production and synchronous tartrazine degradation: Efficiency and mechanism insight

Enhancing of Acetone Purity and Energy Efficiency in the Isopropyl Alcohol (IPA) Dehydrogenation Process Through Design Modifications, Heat Exchanger Integration Simulation, and Reactor Temperature Optimization

Acetone is an essential ingredient in various industries whose demand continues to increase, thus requiring an efficient production method. This study aims to design an acetone production process by dehydrogenating isopropyl alcohol using Aspen HYSYS simulation and Aspen Energy Analyzer energy analysis. The simulation model was developed to enhance the purity of acetone products and improve energy efficiency by optimizing thermodynamic operating conditions. The dehydrogenation process was designed to produce acetone as the main product and hydrogen as a by-product. The basic process was then modified by integrating thermal energy and increasing the reactor operating temperature to improve energy efficiency and product purity. Simulations showed that the process modification resulted in an acetone purity of 99.76%, higher than the base process of 98.46%. In addition, energy savings in the modified process reached 40.89%, higher than the base process of 32.96%, with a reduction in carbon emissions of up to 40.88%. With these results, the modified process proved more efficient than the basic process and aligned with the research objectives. Copyright © 2024 by Authors, Published by Universitas Diponegoro and BCREC Publishing Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Nutritional, Antibacterial and Thrombolytic Prospects of Freshwater Paludomas conica Protein Hydrolysate and Its Anti-Inflammatory Potential in LPS-Induced RAW 264.7 Macrophage Cells.

Chronic inflammation and heme-iron overload can result from bacterial hemolysis. Along with the synthetic drugs, numerous traditional and functional food approaches are equally trialed to eradicate the problem. As a prospective new source of dietary protein hydrolysates, freshwater mollusks (Paludomas conica) have recently drawn huge interest from researchers. In this research, protein hydrolysate (PhPC) of Paludomas conica, prepared by the enzyme digestion method, was analyzed for proximate nutritional and minerals contents and deciphered its suppressive effects on inflammatory gene expression in LPS-stimulated RAW264.7 macrophage cells. The inhibitory action of protein denaturation is also unfolded with established invitro and invivo models. Anti-hemolytic, antibacterial, and thrombolytic effects of PhPC were respectively assessed by H2O2-induced hemolysis of RBCs, the disc diffusion method, and the clot lysis method. The proximate nutritional and mineral contents of PhPC revealed it to be an enriched source of nutrients, crude protein, carbohydrates, Calcium, and Magnesium. Heavy metals were found to be within the prescribed limit. The PhPC suppressed the expression of inflammatory genes, including COX-2, iNOS, IL-6, TNF-α, and IL-1, multifold in LPS-stimulated RAW264.7 macrophages. The inhibition concentrations (IC50) of PhPC in the bovine serum albumin denaturation inhibition test and membrane stabilization tests were 431.39 and 285.25 μg/mL, respectively. The PhPC was discerned to be active against Shigella flexneri, Pseudomonas aeruginosa, and Shigella dysenteriae; its maximum thrombolytic effect was displayed to be 23.72% ± 2.71%. The findings demonstrate that the nutritionally enriched PhPC could be affirmed as an exciting invertebrate anti-inflammatory agent Extending other biological functions needs to be further characterized with its pure protein or protein products.

Development of high-performance inducible and secretory expression vector and host system for enhanced recombinant protein production

The production of lipopolysaccharide (LPS)-free recombinant proteins from culture supernatants is of great interest to biomedical research and industry. Due to the LPS-free cell wall structure and the well-defined secretion factor B (SecB)-dependent secretion pathway, Gram-positive bacteria are a superior alternative to Escherichia coli expression systems. However, the lack of inducible expression systems for high yields has been a bottleneck. To address this, we developed the pKS81 plasmid, featuring the uhpT (glucose-6-phosphate [G6P] transporter) promoter for high expression of recombinant proteins induced by extracellular G6P via a three-component hexose phosphate transport regulatory system (HptARS), the N-terminal SecB-dependent signal peptide sequence for recombinant protein secretion, and the C-terminal 8 × histidine tag for purification by nickel affinity chromatography. We also generated an expression host strain, Staphylococcus aureus LAC9, lacks the uhpT gene and harmful superantigen and leukotoxin genes, allowing for constitutive HptARS activation by extracellular G6P and increased safety, respectively. Using the pKS81 plasmid, we successfully achieved high yields of prokaryotic (staphylococcal leukotoxin E) and eukaryotic (human annexin A2 protein tagged with mouse IgG1) recombinant proteins, up to 900 mg/L. Our newly established inducible and secretory expression system provides for efficient production and easy purification of LPS-free recombinant proteins, making it valuable for biomedical research and industrial applications.

Bactericidal Metal-Organic Gallium Frameworks - Synthesis to Application.

Gallium, a trace metal not found in its elemental form in nature, has garnered significant interest as a biocide, given its ability to interfere with iron metabolism in bacteria. Consequently, several gallium compounds have been developed and studied for their antimicrobial properties but face challenges of poor solubility and formulation for delivery. Organizing the metal into three-dimensional, hybrid scaffolds, termed metal-organic frameworks (MOFs), is an emerging platform with potential to address many of these limitations. Gallium MOFs show improved solubility and antibacterial potency relative to the free metal due to their ability to coload antibiotics and functional biomolecules. Synthetic strategies are equally versatile, with several rapid, cost-effective, and scalable methods available. In this review, we present the advantages and disadvantages of these various synthetic strategies with respect to their antibacterial efficiency, product purity, and reaction control. The activity of gallium-based MOFs against Gram-positive and Gram-negative pathogens in mono- and combinatorial therapeutic settings is discussed in the context of their mechanisms of action and structure-function-performance relationships collated from recent studies. While gallium MOF development as antibacterials is still in its nascent stages, the examples discussed here highlight their potential as a novel class of therapeutics poised to impact the fight against pan-drug-resistant bacterial pathogens.

Meat productivity and beef quality of Black-and-White bulls fed with zinc and selenium in organic form as part of the diet

Increasing the production of high quality ecologically pure animal products, especially beef is one of the main tasks of the agro-industrial complex. In this regard, it is necessary to apply measures for the effective use of genetic resources of both domestic and imported origin, improving feeding systems, using feed additives, biological active substances that stimulate the growth and development of animals. The aim of the study was to determine the effect of the feed additives Plexomin Zn 26 and Plexomin Se 2000, containing in its composition zinc and selenium in organic form, on the slaughter parameters of Black-andWhite bulls, as well as on the quality of the obtained product - beef. For the research, 15-month-old Black-and-White bulls were divided into two groups (control and experimental) of 20 heads each, average live weight was 328.8–329.3 kg. The experiment lasted 91 days. The difference was that the experimental animals were fed with the feed additives Plexomin Se 2000 at a dosage of 1.2 g/head/day and Plexomin Zn 26 at a dosage of 1.2 g/head/day as part of the concentrated part of the diet during the study period. A control slaughter was carried out at the meat processing plant "Orenbeef" for comparative evaluation of meat productivity and beef quality of bulls fattened with and without feed additive. As a result of the research, it was found out that experimental bulls outperformed their control counterparts in live weight at the end of the experiment by 2.5 % (Р≤0.05), pre-slaughter live weight by 2.2 % (Р≤0.05), fresh carcass weight by 4.5 % (Р≤0.01), internal fat weight by 22.1 % (Р≤0.05), slaughter weight by 5.2 % (Р≤0.01), and slaughter yield by 1.62 % (Р≤0.05). Meat from experimental bulls contained 1.35 % more dry matter (Р≤0.05), 0.26 % more fat (Р≤0.05), 32.9 % more glycogen (Р≤0.01), 1.3 % less moisture (Р≤0.05), 7.1 % more oxyproline (Р≤0.05) and 1.92 % more pH (Р≤0.05).

Control of Heat-Integrated Distillation Columns: Review, Trends, and Challenges for Future Research

Heat-integrated distillation columns (HIDiC) are well known for their high energy efficiency, which has been demonstrated through thorough model-based simulation and practical testing. Despite this advantage, HIDiC systems are fundamentally complicated and provide major hurdles, particularly in terms of dynamic control, complicating their industrial implementation. Ongoing research is critical to improving their stability and scalability, allowing for wider incorporation into industrial processes. This review focuses on the fundamental aspects of HIDiC systems, such as heat transfer models, design improvements, experimental research, modelling, simulation, optimization, and process control techniques. This paper summarizes the present status of research and identifies significant technological obstacles that must be overcome to increase the functionality and industrial applications of HIDiC technology. In response to the increased demand for energy-efficient industrial processes, the analysis also investigates current developments in HIDiC control and optimization methodologies. It evaluates several control approaches, both model-based and data-driven, and their capacity to handle the dynamic complexities seen in HIDiC systems. Furthermore, this paper discusses the most recent optimization efforts targeted at improving product purity, operational flexibility, and overall energy efficiency.

Bipolar membrane electrodialysis with isolation chamber enables high‐purity LiOH production with ordinary membranes

AbstractCurrently, bipolar membrane electrodialysis (BMED) is recognized as an eco‐friendly technique to recycle lithium from waste lithium‐ion batteries. However, the application of ordinary bipolar membranes has the disadvantage of unsatisfactory product purity due to undesired ion leakage. Herein, we proposed isolation chamber bipolar membrane electrodialysis (ICBMED) to inhibit coion migration, thereby increasing the purity of the regenerated acid and alkali. The experimental results indicate that 97.7%–99.3% of the LiOH generated by the ICBMED using domestic membranes was generated, which is much greater than the 85.7%–94.4% obtained without an isolation chamber. The total cost of the ICBMED for LiOH production with inexpensive domestic membranes was 1.65$/kg‐LiOH (US) at 400 A/m2, which is lower than the cost of 1.91$/kg‐LiOH (US) for flagship membranes with identical product quality. BMED with an isolation chamber provides a viable solution for acid–base production by balancing product quality and cost.

Recovery of lithium from waste liquid of rock salt brine using aluminum hydroxide precipitation method

An integrated process consisting of Li+ precipitation by Al(OH)3, roasting, water leaching, evaporation, and Li2CO3 precipitation was used to recycle Li+ from the waste liquid of rock salt brine (0.099 g/L Li+). Waste liquid from roc salt brine was discharged wastewater after NaCl crystallization and the removal of impurities in the salt manufacturing plant of the good rock salt mine. The influences of Al3+/Li+ mole ratio, Na+/Al3+ mole ratio, precipitation temperature, and time on the recovery of Li+ were investigated during Li+ precipitation by Al(OH)3 stage. The results showed that the optimal condition was Al3+/Li+ mole ratio = 2.5, Na+/Al3+ mole ratio = 2.2, precipitation temperature of 60℃ (333.15 K) for more than 20 min, whose recovery of Li+ reached 97.25%. The thermodynamic analyses of the simulated Li+–Al+–Mg2+–Cl––H2O system were conducted to construct the potential-pH (φ-pH) diagrams. The results showed that the pH value should be located in the LiCl · 2Al(OH)3 · 2H2O salt region with no formation of Mg(OH)2, which started at pH = ~6.5 and ended at pH from 10.09 to 8.55 as the temperature changed. Subsequently, the Li+precipitate was roasting for the transformation of insoluble LiCl · 2Al(OH)3 · xH2O salt to soluble LiCl, followed by the water leaching to obtain the enriched Li+ solution (1.951 g/L Li+) with Li+ recovery of 85.52%. To meet the requirement of Li2CO3 precipitation, the enriched Li+ solution was evaporated, and Na2CO3 was added to precipitate the Li2CO3 product after SO4 2–, Ca2+, and Mg2+ removal. The total recovery of Li+ was 66.69% after the experimental process, and the purity of Li2CO3 product was 99.3%, which can be regarded as industrial-grade Li2CO3. In conclusion, the success in lithium recovery using the aluminum hydroxide precipitation method provided a new perspective for preparing Li2CO3 from the waste liquid of rock salt brine, which could be considered as a newly developing lithium resource to meet the dramatically increasing demand for lithium in new energy vehicle industry.