Blood purification technology is one of the main treatment methods to prolong the life of severe patients. The traditional blood purification membrane materials have the disadvantages of non-selective adsorption, single pore size and low permeability, and need to develop new and efficient blood purification membrane materials. To explore the application of a new type of nanofiber membrane in blood purification of critically ill patients and compare and analyze its nursing methods. When the experimental subjects are performing blood purification, a new nanofiber membrane material is used in the purification machine. The control group adopted conventional nursing methods, while the experimental group adopted targeted nursing methods based on the nursing methods of the control group. After intervention, the eight indicators of quality of life in the experimental group were significantly improved (P < 0.05), for example, the score of physiological function was increased from 41.042 ± 7.625 to 54.236 ± 2.544, and the energy status was increased from 46.094 ± 7.192 to 59.768 ± 4.241. The physiological and biochemical indexes of both groups tend to be normal, but the experimental group is more obvious. The patients in the experimental group were more satisfied with the nursing services provided in the process. The new blood purification method of nanofiber membrane can overcome the shortcomings of traditional purification and remove toxic small and medium-sized molecules to the maximum extent.