Pure Mycelium Research Articles

Modulation of flexibility and shape recovery of living Schizophyllum commune pure mycelium material by hydration

Pure mycelium materials (PMMs) are novel sustainable materials that could for instance be used as a foam, cellular scaffold, or as an alternative for textile, leather, or meat. Here, mechanical properties of the PMM of the fungus Schizophyllum commune were studied when exposed to different levels of relative humidity (RH) and after submersion in water. Exposure to increasing RH resulted in a more flexible PMM. For instance, exposure to 95 % RH decreased the Young’s modulus from 467 to 3.7 MPa and increased elongation at break from 1 % to 18.5 %. Similar properties were found after submersion in water, enabling reshaping the PMM in a mould. Notably, the PMM showed shape memory when it was exposed to cycles of drying in a 90° angled shape, wetting, drying at 180°, and submersion in water. The material reached a mean percentage of shape recovery completeness of 49 %, 55 %, and 66 % in the first, second and third cycle, respectively. The capacity to store and release potential energy was not affected during these cycles. Together, a living PMM with hygromorphic shape memory is described for the first time, which may be used for instance in smart switches or valves.

Epidemiological etiology of Erysiphe sp. and putative viral and phytoplasma-like symptoms in Ayocote bean (Phaseolus coccineus)

Introduction/Objective. Ayocote bean (Phaseolus coccineus) has potential as a source of resistance in breeding programs because it exhibits greater tolerance to plant pathogens than P. vulgaris. However, its sanitary characterization is insipient; therefore, the purpose of this work was to carry out an etiological-epidemiological diagnosis, with emphasis on presumptive symptoms of viral and phytoplasmic organisms, and a typical fungal signs of powdery mildew. Materials and Methods. A plot (50 x 62 m) of flowering Ayocote bean was selected. It was divided into 80 (8 x 10) quadrats (6 x 6 m) and 720 subquadrats (2 x 2 m). From 25 plants with powdery-mildew-type leaf symptoms, mycelium was collected with adhesive tape for light microscopy observation and taxonomic identification. Length-width measurements were made on 60 conidia. Pure mycelium collected in situ and ex situ from 1-5 leaflets/plant was used for genomic analysis by PCR with universal primers ITS1 and ITS4. Samples were sequenced in Macrogen Inc. Korea. A total of 63 plants and 121 trifoliate leaves with viral and phytoplasmic symptoms were collected by direct sampling. In 88/121 samples, genomic analysis was performed by PCR with universal primers for Potyvirus (1), Begomovirus (2), and Phytoplasmas (1). Sequence editing and analysis were performed in SeqAssem and BLASTn/GenBank. Phylogenetic constructions were developed in Mega 11 with MUSCLE, Maximum Likelihood (ML), and HKY substitution model (1000-Bootstrap). Putative powdery mildew severity (%), flower damage (%), Macrodactylus sp. adult density, and plant vigor (%) were evaluated in 80 quadrats (3subquadrats/quadrat) with App-Monitor®v1.1 configured with a 5-class scale. In GoldenSurfer® v10, Kriging geostatistical analysis was performed to determine the spatial interrelationship between these variables. Results. Erysiphe vignae was identified as associated with powdery mildew of P. coccineus. The fungus, with hyaline, ovoid to ellipsoid conidia measuring 31.74 ± 0.3419 μm x 15.11 ± 0.1579 μm, without the presence of fibrosin bodies, had 100% genomic homology. This is the first report in Mexico. With average July-August temperature and relative humidity of 16.3 °C (±5.8) and 92.8 % (±10.7), respectively, powdery mildew leaf incidence and severity were 65.3 and 22.7 % (±16.9, range: 0 - 66.5 %), respectively. The most inductive focus (60-80 % severity) had an aggregate e 4-quadrat pattern (96 m2, lag = 4 and σ2-s = 450). Inoculum dispersal was significantly associated with dominant North-South winds and plant vigor (lag = 4 and σ2-s = 470). Flower damage was inconclusive in its spatial association with powdery mildew and Macrodactylus sp. suggesting uncorrelated events. No Potyvirus, Begomovirus, or Phytoplasmas were detected associated with yellowing, leaf distortion, mosaic, internode shortening, and other symptoms observed in situ. This confirms the relative tolerance/resistance reported for P. coccineus. Conclusion. E. vignae (Erysiphales: Erysiphaceae) associated with P. coccineus is reported for the first time in Mexico with moderate to intense epidemic level, which indicates its susceptible condition to this fungus. However, negative results for Potyvirus, Begomovirus, and Phytoplasmas, validate the apparent tolerance/resistance of P. coccineus to these organisms.

Effect of the biomass of the cyanobacterium Nostoc commune on the growth of <i>Fusarium oxysporum </i>"<i>In vitro</i>"

The cyanobacteria Nostoc commune contains bioactive compounds with antimicrobial activity and high potential to control of phytopathogens. The objective of this work was to determine the in vitro effect of sonicated biomass of these cyanobacteria on the growth of Fusarium oxysporum. The biomass of N.commune was dissolved in sterile distilled water and subjected to centrifugation processes and ultrasound-assisted cell rupture. The antifungal effect was evaluated using the disk diffusion technique (Kirby-Bauer) in a PDA (Potato Dextrose Agar) medium. In the center of the Petri dish, an agar disk (0.7 cm) with pure mycelium of the fungus was placed in the center of the Petri dish and, around it, four filter paper disk impregnateed with the algae extract (0.7 cm) were placed. The radial growth of the pathogen was measured in the presence and absence of N. commune, until the control covered the entire Petri dish to assess the Percentage of Radial Inhibition of the pathogen (PICR %). The growth rate of the fungus was lower in the initial stages of measurement and the PICR fluctuated between 33 and 58%. However, there were no inhibition halos in the growth of the fungus, it is assumed that the antifungal effect of the cyanobacteria was not permanent over the time, but it could be potentiated by improving the extraction process of the extract.

Water behavior, equilibrium, and migration of a biomaterial made of pure mycelium

Water behavior, equilibrium, and migration of a biomaterial made of pure mycelium

Functional fungal pellets self-immobilized by mycelium fragments of Irpex lacteus WRF-IL for efficient degradation of sulfamethazine as the sole carbon source

In order to achieve an efficient microbial material with dual functions of self-immobilization and sulfamethazine (SMZ) degradation, this study explored the pelletization technique utilizing mycelium fragments of Irpex lacteus WRF-IL and systematically examined the pellets formation conditions and degradation capability. The Box-Behnken design results demonstrated that pure mycelium fragments, broken by frosted glass beads, could be rapidly self-immobilized to form white rot mycelial pellets (WRMPs) within 24 h, serving as the pelleting core. These WRMPs could completely remove SMZ as the sole carbon source within 20 h. The addition of sucrose expedited this process, achieving complete removal within only 14 h. Kinetic analysis showed that WRMPs could potentially remove SMZ at higher concentrations (>25 mg/L). Biodegradation was the primary pathway of SMZ removal. Seven intermediates were identified by QTOF LC/MS, and three transformation pathways initiated by SO2 overflow, molecular rearrangement, and aniline moiety oxidation were deduced.

Growing sustainable materials from filamentous fungi

The urgent need to transition to a more sustainable way of life is driving the development of natural and sustainable materials. One such example is mycelium materials, which are partially or solely composed of hyphae, the vegetative life form of filamentous fungi. Mycelium materials offer a sustainable and environmentally friendly alternative to synthetic materials like plastics and to animal leather, by minimizing waste, reducing CO2 emissions and promoting a circular economy. In their natural habitat, fungi play a vital role in maintaining balanced forest ecosystems by decomposing and recycling organic matter. From a material application perspective, the mycelium itself is now being considered as a product that can be easily generated by cultivating the fungus on renewable biomass feedstocks. Mycelium-based composites and pure mycelium materials can be produced through different fermentation techniques, including solid-state (SSF), liquid-state submerged (LSF) and liquid-state surface fermentation (LSSF). Interestingly, the properties of mycelium materials are highly tuneable by altering the growth and post-growth process parameters, the choice of fungal species and the used substrate. Although the link between the biology of a living organism and the emergent material characteristics thereof offers great opportunities for fine-tuning material properties, a current lack of understanding of the underlying scientific principles makes industrial scalability and product reproducibility a challenging step. In this article, we discuss the opportunities and challenges of mycelium materials as a novel class of biomaterials.

Mycelium as sustainable textile material – review on recent research and future prospective

PurposeMycelium is an upcoming bio-based alternative material that has various applications in different industries. Mycelium materials used as composites, leather, construction materials and some are even available for commercial purposes. However, there was not much research found when it came to the application of mycelium as a textile alternative. The purpose of this paper is to examine the potential of mycelium in the textile industry and its possible applications.Design/methodology/approachThis review consolidates literature that refers the two major methods used in fungal mycelium production namely; as a composite and as a pure self-grown mycelium sheet. The study compared the current research status in this respective field and reported the scope in the pure mycelium development.FindingsThe results of the review reported that several research works are performed in composite production with different feedstock. The production methods and product development steps were well established for several applications from home utilities to construction materials. Whereas, in the case of self-grown mycelium sheet production only limited research works were found. Though the possibilities of engineered composite sheets are developed with various properties, research on self-grown pure mycelium sheets are at infant stage. Sensitive production methods, lower tensile, tearing, poor handle properties with brittle structure and non-uniformity in thickness are noted as limitations. Sustainable nature, self-grown three-dimensional nano-fibril network with porous structure are found to be advantageous.Originality/valueThe solid culture method was identified as a potential method to develop a sheet-like self-grown mycelium with different dimensions. The review results clearly show the lack of research in the direct application of self-grown pure mycelium area concerning feedstock material, fungal species selection and characterization of the developed product. Addressing the existing limitations will yield a sustainable textile material for fashion and textile industry with great potential.

Fungal Engineered Living Materials: The Viability of Pure Mycelium Materials with Self‐Healing Functionalities

AbstractEngineered living materials (ELMs) composed entirely of fungal cells offer significant potential due to their functional properties such as self‐assembly, sensing, and self‐healing. Alongside rapid developments in the ELM field, there is significant and growing interest in mycelium materials, which are made from the vegetative part of filamentous fungi, as a potential source of advanced functional materials. In order to advance the development of fungal ELMs that utilize the organism's ability to regenerate as self‐repair, new methods for controlling and optimizing mycelium materials are needed, as well as a better understanding of the biological mechanisms behind regeneration. In this study, pure mycelium materials are fabricated for use as leather substitutes, and it is found that chlamydospores, thick‐walled vegetative cells formed at the hyphal tip, may be the key to the material's self‐healing properties. The results suggest that mycelium materials can survive in dry and oligotrophic environments, and self‐healing is possible with minimal intervention after a two‐day recovery period. Finally, the study characterizes the mechanical recovery and physical properties of damaged and healed samples, allowing for the first characterization of fungal ELMs.

Cultivation and determination of nutrient contents of an edible wild Thai Hymenopellis

Hymenopellis species are taxonomically well-studied and widely distributed in tropical and temperate regions. It is well-documented in eastern and north America and can also be found in several Asian countries, including Thailand. In this study, we collected a wild Hymenopellis specimen in northern Thailand. The morphological and phylogenetic analyses inferred from a combination of the internal transcribed spacers (ITS) and the large subunit (nrLSU) of ribosomal DNA confirmed the taxonomic placement of the collected specimen to the genus Hymenopellis. Limited studies have documented the cultivation of Hymenopellis species. So far, only China commercially cultivates this mushroom. Pure mycelium was isolated from the internal tissues on malt extract agar (MEA). Sorghum grain was used in spawn preparation. Spawns were inoculated in bags of four different substrate formulas, and sawdust was used as the main substrate and other additives. The cultivation process was conducted for four months. Hymenopellis sp. (MFLU 19-1658) has an average yield ranging from 10.8±17.9 to 115.5±73.5 grams of basidiomata per substrate formula with an average biological efficiency ranging from 3.0±5.0 to 32.1±20.4% with the highest values exhibited by (A) sawdust (98%) + CaO (1%) + gypsum (1%), followed by (B) sawdust (30%) + corncobs (60%) + rice bran (8%) + CaO (1%) + gypsum (1%). The nutrient contents of the cultivated basidiomata were also determined in this study. The results indicated that the obtained basidiomata contained carbohydrate, crude fat, crude fiber, and protein at 49.2, 8.0, 16.5, and 17.8%, respectively. This study is the first to document the nutrient contents of the genus Hymenopellis.

Loop-Mediated Isothermal Amplification (LAMP) and SYBR Green qPCR for Fast and Reliable Detection of Geosmithia morbida (Kolařik) in Infected Walnut.

Walnut species (Juglans spp.) are multipurpose trees, widely employed in plantation forestry for high-quality timber and nut production, as well as in urban greening as ornamental plants. These species are currently threatened by the thousand cankers disease (TCD) complex, an insect–fungus association which involves the ascomycete Geosmithia morbida (GM) and its vector, the bark beetle Pityophthorus juglandis. While TCD has been studied extensively where it originated in North America, little research has been carried out in Europe, where it was more recently introduced. A key step in research to cope with this new phytosanitary emergency is the development of effective molecular detection tools. In this work, we report two accurate molecular methods for the diagnosis of GM, based on LAMP (real-time and visual) and SYBR Green qPCR, which are complimentary to and integrated with similar recently developed assays. Our protocols detected GM DNA from pure mycelium and from infected woody tissue with high accuracy, sensitivity, and specificity, without cross-reactivity to a large panel of taxonomically related species. The precision and robustness of our tests guarantee high diagnostic standards and could be used to support field diagnostic end-users in TCD monitoring and surveillance campaigns.

Current state and future prospects of pure mycelium materials

In the context of the ongoing transition from a linear to a circular economy, ecologically friendly renewable solutions are put in place. Filamentous fungi can be grown on various organic feedstocks and functionalized into a range of diverse material types which are biobased and thus more sustainable in terms of their production, use and recycling. Pure mycelium materials, consisting only of mycelial biomass, can adopt versatile properties and appear promising as a substitute for current petrochemically produced polymeric materials or, in the case of myco-leather, as a substitute for animal-based leather. In recent years, a handful of private companies have been innovating to bring products based on pure mycelium materials to the market while scientific interest in these promising biomaterials is now starting to gain momentum. In this primer, we introduce pure mycelium materials, frame different production methods, review existing and potential future applications, thereby offering a vision on future advances for this emerging fungi-based technology.

Collection and Characterization of Wood Decay Fungal Strains for Developing Pure Mycelium Mats.

Wood decay fungi (WDF) seem to be particularly suitable for developing myco-materials due to their mycelial texture, ease of cultivation, and lack of sporification. This study focused on a collection of WDF strains that were later used to develop mycelium mats of leather-like materials. Twenty-one WDF strains were chosen based on the color, homogeneity, and consistency of the mycelia. The growth rate of each strain was measured. To improve the consistency and thickness of the mats, an exclusive method (newly patented) was developed. The obtained materials and the corresponding pure mycelia grown in liquid culture were analyzed by both thermogravimetric analysis (TGA) and scanning electron microscopy (SEM) to evaluate the principal components and texture. TGA provided a semi-quantitative indication on the mycelia and mat composition, but it was hardly able to discriminate differences in the production process (liquid culture versus patented method). SEM provided keen insight on the mycelial microstructure as well as that of the mat without considering the composition; however, it was able to determine the hyphae and porosity dimensions. Although not exhaustive, TGA and SEM are complementary methods that can be used to characterize fungal strains based on their desirable features for various applications in bio-based materials. Taking all of the results into account, the Fomitopsis iberica strain seems to be the most suitable for the development of leather-like materials.

Accumulation and identification of secondary metabolites from the fungus Diaporthe (Phomopsis) helianthi Munt.-Cvet. et al.

The study presents variability in the qualitative composition and quantitative indicators of secondary mycelium metabolites of the fungus Diaporthe (Phomopsis) helianthi Munt.-Cvet. et al., the most harmful plant pest of Helianthus annuus L. Toxicity of secondary metabolites of D. helianthi was analyzed by determining the average length of seedling shoots of Triticum aestivum L. test object grown on fungus filtrates of different cultivation dates. The maximum toxic effect of D. helianthi was recorded during the germination of Triticum aestivum L. seedlings on a 17-day filtrate of pure mycelium culture of the fungus. The mean length of wheat seedlings in this variant of the experiment decreased by 3.5 times compared with the control and 3.3 times compared with the filtrate of the culture medium of 7 days of cultivation. High-performance liquid chromatography (HPLC) identified secondary metabolites of D. helianthi as fomosin, fomopsolides, cytosporones, and xanthones. There was a redistribution of secondary metabolites due to increase in number of cytosporones, decrease in content of fomopsolides, and termination of xanthone synthesis with increasing time of cultivation of fomopsis mycelium, under conditions of relatively stable indicators of fomosin content.

Acoustic evaluation of mycological biopolymer, an all-natural closed cell foam alternative

This research examines the use of a novel new renewable resource in acoustic absorption applications that requires no glues or other artificial binders. Hence the proposed material provides a sustainable and environmentally friendly alternative to construction industry for acoustic absorption panels. The material being tested is an all-natural biopolymer that consists entirely of pure fungal mycelium that is cultured at elevated temperatures from 30 to 35 Cent. in an elevated carbon-dioxide rich environment, from three to seven percent, which in combination suppresses the fruiting bodies. This new biopolymer provides an alternative to closed cell foams and synthetic honeycombs that are suitable for a wide range of applications from fire-resistant bio-leather, textile support foams used in shoes and clothing, scaffolding for medical bio-organs and vegetarian meat. This study examines the acoustical absorption properties of this material over the frequency range from 350 Hz to 4 kHz. The results of the study indicate this new class of a pure mycelium foam provides a sustainable promising bio-based all-natural fiber alternative for acoustic shielding alternative to the traditional acoustic absorbers that are most commonly constructed with petroleum based glues and synthetic fibers.

Fully Bio-Based Hybrid Composites Made of Wood, Fungal Mycelium and Cellulose Nanofibrils

Novel hybrid panel composites based on wood, fungal mycelium, and cellulose nanofibrils (CNF) were developed and investigated in the present study. In one set of experiments, mycelium was grown on softwood particles to produce mycelium-modified wood which was then hybridized with various levels of CNF as binder. The other set of experiments were conducted on unmodified wood particles mixed with CNF and pure mycelium tissue. It was found that the composites made of mycelium-modified wood and CNF resulted in enhanced physical and mechanical properties compared to the ones made by physically mixing wood, mycelium, and CNF. Scanning electron microscopy (SEM) images showed that mycelium modification covered wood particles with a network of fungal hyphae whereas CNF formed a uniform mycelial film over wood particles. Mycelium modification had a significant effect on reducing water absorption and thickness swelling of the hybrid composites and CNF increased the modulus of rupture and modulus of elasticity, optimally at 2.5% addition. We also present results and analysis pertaining to the development of unique lightweight composite systems with physical and mechanical properties optimized at 5% CNF addition with potential to be used in packaging and furniture applications.

Mechanical behavior of mycelium-based particulate composites

This work investigates the mechanical behavior of mycelium composites reinforced with biodegradable agro-waste particles. In the composite, the mycelium acts as a supportive matrix which binds reinforcing particles within its filamentous network structure. The compressive behavior of mycelium composites is investigated using an integrated experimental and computational approach. The experimental results indicate that the composite mimics the soft elastic response of pure mycelium at small strains and demonstrates marked stiffening at larger strains due to the densification of stiff particles. The composite also exhibits the characteristic stress softening effect and hysteresis under cyclic compression previously observed for pure mycelium. To gain further insight into the composite behavior, a three-dimensional finite element model based on numerical homogenization technique is presented. Model validation is performed by direct comparison with experiments, and a parametric study of the effect of mycelium density and particle size is discussed.

DISCOVERY OF ENTOMOPATHOGENIC FUNGI Cordyceps takaomontana AT LANGBIAN MOUNTAIN, LAM DONG, VIET NAM

The stromata of Cordyceps sp. were found on the Langbian Mountain – Da Lat, Vietnam at the height of 1.650 meter above sea level, on the larva of Lepidoptera. Stromata were lemon-yellow, clavate to elongated clavate, arising from a white pseudosclerotium. The fertile head was on the top part of stromata, darker coloured in comparison to the stipe. Perithecium was narrowly ovoid, superficial and forming dark yellow punctate on the surface of stromata. Ascus cylindrical with semi- spherical cap. Ascospores were cylindric, truncated and separately after discharge from the ascus.Pure culture was isolated on Potato Glucose Agar (PGA) medium: white colony in young and yellow in old. The isolated mycelium was not homogenous in thickness and in growth rate at the peripheral area. Conidiophores were phialide, tapering to both apexes. Conidia had elliptical shape and form into chains after maturation.DNA was isolated, then purified from pure mycelium and used to amplifying the nrLSU (nuclear ribosomal large subunit) sequence. The amplified products were used for sequencing, proof-reading by some professional solfwares before combining with other nrLSU sequences. Then this database was used to search for the suitable evolution model as well as to construct the phylogenetic trees.The results of phylogenetic analysis completely supported the morphological classification: DL0038A and DL0038B were Cordyceps takaomontana.

In vitro Inhibition of Soilborn Phytopathogens Treated With Swine Wastewater

Swine wastewater (SWW) is a residue from pig farming that presents a high load of nutrients and organic matter. The appliance of organic matter in soil alters the microbial dynamic and may suppress soilborn phytopathogens. This study aimed at evaluating the inhibition on mycelial growth of Sclerotinia sclerotiorum and Sclerotium rolfsii in vitro under SWW doses. Hereupon, three kilograms of a soil classified as red dystroferric latosol was collected and sieved. Half of it was autoclaved. SWW was incorporated at doses of 0 mL, 2.5 mL, 5 mL, 10 mL and 20 mL in both soil conditions, autoclaved and not autoclaved. Afterwards, 130 grams of each soil was separately put into Petri plates above what a thin layer (≅ 5 mL) of Water-Agar (2%) medium was carefully spread over. Above this agar layer, one disk (6 mm diameter) of pure mycelium from each fungal grown in Potato Dextrose Agar medium was individually placed on the center of each plate. Daily evaluations on mycelial growth measuring were taken and ended when mycelium in control plates (without SWW addition) reached plate borders. Results indicated that in autoclaved soil condition, the inhibition was proportional to the dose, what is to say that the higher the dose the less the mycelial growth. In not autoclaved soil there was no significant difference among treatments, suggesting stimuli on suppression effect for both pathogens caused by SWW. In addition, the confirmed potential of SWW as a suppressor of S. sclerotiorum and S. rolfsii leads to promising investigations on other phytopathogens hard to control.

Evaluation of Yield, Heavy Metals and Vitamins Compositions of Pleurotus Pulmonarius (Fries) Quell Fruit Bodies Cultivated on three Deciduous Tree Logs

Studies were conducted to determine the yield potential, vitamins and heavy metals concentrations of Pleurotus pulmonarius (Fries) Quell. fruit bodies cultivated on different wood logs. Pure mycelium culture of P. pulmonarius was aseptically multiplied using sorghum grains. Fully colonized spawn was used to inoculate Mangifera indica, Dacryodes edulis and Treculia africana logs and incubated in the dark room of a mushroom house at 27°C ± 2. Data were subjected to analysis of variance (ANOVA), while comparison between different means were done using Duncan multiple range test (DMRT) at p<0.05 level of significance. Fruit body primordia of the oyster mushroom were first observed in D. edulis logs, followed by T. africana and then M. indica logs after 9, 10 and 12 days of inoculation respectively. T. africana gave the highest yield (156.0967 gm/kg) and Biological efficiency (0.836%) of P. pulmonarius fruit bodies while D. edulis was lowest at 62.3667 gm/kg and 0.200% biological efficiency. Fruit bodies of M. indica had the highest (3.19mg/kg) Zn content; slightly higher than those of T. africana (3.14mg/kg), while fruit bodies from D. edulis gave the lowest Zn (2.96mg/kg) concentration. Fe was significantly (p < 0.05) higher than other heavy metals studied in all the fruit body samples from the log substrates. Mushrooms of D. edulis gave the highest (184.87mg/kg) Fe concentration while those of M. indica gave the lowest (182.91mg/kg). Results showed that D. edulis fruit bodies gave the highest vitamin A content (4.33mg/100g) while M. indica (4.17mg/100g) gave the lowest. However, thiamine content of the mushroom was 0.13mg/100g in D. edulis and T. africana but 0.12mg/100g in M. indica. Mushroom growers should harness the wisdom of this wonderful innovation by utilizing wood logs first, before they can be used for other domestic purposes, such as fire wood.

Nutritional and Bioactive Compounds Evaluation of Pleurotus pulmonarius (Freis) Quell Fruit bodies Grown on Different Wood Logs in Abia State, Nigeria

Abstract This study was conducted to determine the nutritional and bioactive compounds composition of Pleurotus pulmonarius fruit bodies cultivated on tree logs of Dacryodes edulis, Mangifera indica and Treculia africana. Pure mycelium culture of P. pulmonarius was aseptically bulked in sorghum grains. Logs were cut into average length of 18 cm with inoculation holes of 3 cm × 15 mm diameter; using High Speed Drill (HSD) of 5 drill bit and allowed to decompose for 8months. During mushroom cultivation, logs were soaked in tap water for 24 hrs and pasteurized at 80°C in an improvised metallic drum (IMD) for 1hour; using cooking gas as heat source and allowed to cool overnight. 10 g of grain based spawn was inserted into 2/3 of each hole by way of inoculation and sealed with sterile polybag for mycelium incubation. Polybags were cut open after spawn run following primordial initiation. Fruit bodies were harvested at maturity, sun-dried ground and packed in airtight container prior to further analysis. Data were analyzed using Analysis of Variance (ANOVA) and mean separation by Duncan Multiple Range Test (DMRT) while levels of significance were determined at 5%. Results indicate that P. pulmonarius fruit bodies harvested from various tree logs were significantly different p<0.05 in their nutritional and bioactive compounds composition. Fruit body samples were rich in protein, carbohydrates, Na, K, and Ca. It was also observed that fruit bodies contained significant amount of Alkaloids, Tannins and Saponins; and could be useful in drug synthesis. Therefore, adopting this technique in oyster mushroom cultivation would lead to more jobs creation and food security; but this must be done with careful regulations to avoid indiscriminate felling of trees.