Pure Alkaloids Research Articles

Enantioselective Ammonium Ylide Mediated One‐Pot Synthesis of Highly Substituted γ‐Butyrolactones

AbstractAn ammonium ylide mediated access towards trans‐β,γ‐disubstituted, all‐trans‐α,β,γ‐trisubstituted, and α,α,β,γ‐tetrasubstituted γ‐butyrolactones bearing a broad variety of functionalities was developed. Starting from widely accessible benzylidene Meldrum's acid derivatives and α‐bromo carbonyl compounds, γ‐butyrolactones were obtained in yields between 32–99% with up to excellent diastereoselectivities (>95:5) via a DABCO‐mediated [2+1] annulation. Utilization of enantiomerically pure cinchona alkaloid derivatives enables the first asymmetric ammonium ylide mediated method to provide (3R, 4R)‐β,γ‐disubstituted and (2R, 3R, 4R)‐α,β,γ‐trisubstituted γ‐butyrolactones in moderate to good yields with up to very good enantiomeric ratios (97:3). The scalability of the transformation was proven while determining the absolute configuration.magnified image

A Review of Bioinsecticidal Activity and Mode of Action of Plant

Plants contain many bioactive compounds as a means of defense against insects. Some plants produce toxic compounds that could affect or kill the insect completely. These natural compounds are an alternate for synthetic insecticides that cause many problems to the environment and human health. Hence, there is an urgent need for botanical insecticide which is eco-friendly and control insects in short duration. The secondary metabolites namely alkaloids have significant effects on insects. The alkaloids can affect insects through biological action such as disturbing the cellular and physiological process via redox imbalance and hormonal regulation. In this review, we present data on sub lethal and lethal toxicity caused by the various species and alkaloids obtained from the plants. The pure alkaloids and extracts of various plants cause toxicity towards insects which are considered as the important point of view economically.

Antiparasitic properties of leaf extracts derived from selected Nicotiana species and Nicotiana tabacum varieties

Within the traditional pharmacopeia, tobacco (Nicotiana spp.) is often cited as an efficient pesticide. This activity is generally attributed to nicotine, but tobacco plants contain other alkaloids that could potentially contribute to this effect. In this study, we tested methanolic extracts of N. glutinosa, N. glauca, N. debneyi, and N. tabacum (putrescine N-methyltransferase line, burley TN90 and Stella, Virginia ITB 683 and K326), selected according to alkaloid content. Their antiparasitic activity was evaluated in bioassays against adult fleas (Ctenocephalides felis), blowfly (Lucilia cuprina) larvae, nematodes (Caenorhabditis elegans), and ticks (Rhipicephalus sanguineus larvae and adults, Ixodes ricinus nymphs). None of the extracts killed fleas and blowfly larvae effectively at the concentrations tested. Only N. tabacum K326 and N. glutinosa exhibited moderate anthelmintic activity. All extracts significantly repelled R. sanguineus ticks, but not I. ricinus, and the nicotine-rich extracts rapidly knocked down all tick species and stages at high concentrations. The link between nicotine and tick knockdown was confirmed by successfully testing the pure alkaloid at concentrations found in the tobacco extracts. In contrast, repellent activity could not be correlated to the individually tested alkaloids (nicotine, nornicotine, anabasine, anatabine), although anatabine and nornicotine were active in the tick bioassay at high concentrations.

Performances of Alkaloid Extract from Rauvolfia macrophylla Stapf toward Corrosion Inhibition of C38 Steel in Acidic Media.

Alkaloid extract from Rauvolfia macrophylla Stapf (AERMS) was studied as the corrosion inhibitor for C38 steel in 1 M HCl and 0.5 M H2SO4 using electrochemistry and surface analysis. The corrosion inhibition was efficient and proceeds via adsorption of AERMS on the steel surface due to the active functional groups present in the molecules. AERMS acts as a mixed inhibitor in HCl and as a cathodic inhibitor in H2SO4. In H2SO4 corrosive medium, the presence of iodides improves the adsorption of the alkaloid molecules by reducing the surface charge of the electrode and thus substantially decreases the corrosion rate. Two pure alkaloids (tetrahydroalastonine (THA) and perakine (PER)) were quantitatively isolated from AERMS, and their anticorrosive properties for C38 steel in 1 M HCl and 0.5 M H2SO4 were evaluated. THA showed the highest efficiency while the performance of PER was less important compared to the extract. This confirms that the efficiency of AERMS was the result of the complementary action of the chemical compounds present in the extract.

Differentiated Effects of Secondary Metabolites from Solanaceae and Brassicaceae Plant Families on the Heartbeat of Tenebrio molitor Pupae.

The usage of insects as model organisms is becoming more and more common in toxicological, pharmacological, genetic and biomedical research. Insects, such as fruit flies (Drosophila melanogaster), locusts (Locusta migratoria), stick insects (Baculum extradentatum) or beetles (Tenebrio molitor) are used to assess the effect of different active compounds, as well as to analyse the background and course of certain diseases, including heart disorders. The goal of this study was to assess the influence of secondary metabolites extracted from Solanaceae and Brassicaceae plants: Potato (Solanum tuberosum), tomato (Solanum lycopersicum), black nightshade (Solanum nigrum) and horseradish (Armoracia rusticana), on T. molitor beetle heart contractility in comparison with pure alkaloids. During the in vivo bioassays, the plants glycoalkaloid extracts and pure substances were injected at the concentration 10−5 M into T. molitor pupa and evoked changes in heart activity. Pure glycoalkaloids caused mainly positive chronotropic effects, dependant on heart activity phase during a 24-h period of recording. Moreover, the substances affected the duration of the heart activity phases. Similarly, to the pure glycoalkaloids, the tested extracts also mainly accelerated the heart rhythm, however S. tuberosum and S. lycopersicum extracts slightly decreased the heart contractions frequency in the last 6 h of the recording. Cardioacceleratory activity of only S. lycopersicum extract was higher than single alkaloids whereas S. tubersoum and S. nigrum extracts were less active when compared to pure alkaloids. The most cardioactive substance was chaconine which strongly stimulated heart action during the whole recording after injection. A. rusticana extract which is composed mainly of glucosinolates did not significantly affect the heart contractions. Obtained results showed that glycoalkaloids were much more active than glucosinolates. However, the extracts depending on the plant species might be more or less active than pure substances.

Isolation and Identification of Alkaloids from Poisons of Fire Salamanders ( Salamandra salamandra).

Fire salamanders ( Salamandra salamandra) are conspicuously colored amphibians secreting a skin poison that contains unique steroid alkaloids such as samandarine (1) and samadarone (2), exhibiting toxic as well as antimicrobial activities. Because of their antipredatory and anti-infectious functions, alkaloids from Salamandra poison are of interest with regard to the threat that the lethal fungus Batrachochytrium salamandrivorans ( Bsal) poses to salamanders. Nevertheless, reliable data on the biological activity of Salamandra alkaloids are scarce, in part due to the difficulty to obtain and study those substances. Thus, isolation of pure salamander alkaloids is an important task that might directly contribute to the understanding of Bsal infections. Here we present a noninvasive isolation procedure for samandarine (1) and O-acetylsamandarine (3), as well as for two new alkaloids, O-3-hydroxybutanoylsamandarine (4) and samanone (6), using HPLC. For the first time, high-field NMR data are presented for these alkaloids. Analysis using GC/MS and ESI+-MS, provided important information on the structural variability of these salamander alkaloids.

The effects of TeaCrine\xae and caffeine on endurance and cognitive performance during a simulated match in high-level soccer players

BackgroundTheacrine (1,3,7,9-tetramethyluric-acid) is a pure alkaloid with a similar structure to caffeine and acts comparably as an adenosine receptor antagonist. Early studies have shown non-habituating effects, including increases in energy and focus in response to Teacrine®, the compound containing pure theacrine. The purpose of this study was to determine and compare the effects of Teacrine® and caffeine on cognitive performance and time-to-exhaustion during a simulated soccer game in high-level male and female athletes.MethodsMale and female soccer players (N = 24; MAge = 20.96 ± 2.05y, MMaleVO2max = 55.31 ± 3.39 mL/O2/kg, MFemaleVO2max = 50.97 ± 3.90 mL/O2/kg) completed a 90-min simulated treadmill soccer match over four randomized sessions (TeaCrine®, caffeine, TeaCrine® + caffeine, placebo). Cognitive testing at halftime and end-of-game including simple reaction time (SRT), choice RT (CRT), and cognitive-load RT with distraction questions (COGRT/COGRTWrong) was performed, with a run time-to-exhaustion (TTE) at 85% VO2max following end-of-game cognitive testing. Session times and pre-exercise nutrition were controlled. RM-MANOVAs with univariate follow-ups were conducted and significance was set at P < 0.05.ResultsTTE trended towards significance in TeaCrine® and TeaCrine® + caffeine conditions compared to placebo (P < 0.052). A condition main effect (P < 0.05) occurred with faster CRT in caffeine and TeaCrine® + caffeine compared to placebo. COGRTWrong showed a significant time main effect, with better accuracy at end-of-game compared to halftime (P < 0.05). A time x condition interaction in SRT (P < 0.05) showed placebo improved from halftime to end-of-game.ConclusionsThe 27–38% improvements in TTE reflect increased performance capacity that may have important implications for overtime scenarios. These findings suggest TeaCrine® favorably impacts endurance and the combination with caffeine provides greater benefits on cognitive function than either supplement independently.

Chemical composition and antibacterial properties of Peganum harmala L.

Objective:The present study was conducted to investigate antibacterial properties of fruit and flower of Peganum harmala.Material and Methods:Column chromatography, followed by preparative thin layer chromatography (TLC) was used for final purification. The structure of pure alkaloids was determined using spectroscopic methods (1H-NMR, 13C-NMR, UV and MS). Smoke and extract of total alkaloids were investigated for antimicrobial activity against five different microorganisms (standards and hospital isolates). The antibacterial activity was evaluated using disc diffusion assay and minimum inhibitory concentration (MIC) was determined by serial dilution methods.Results:Chemical investigation of the chloroform extract of ripe fruit and flower of P. harmala led to identification of three alkaloids in ripe fruit and two alkaloids in the flower and leaves of this plant. Alkaloids identified in ripe fruit were harmine, peganine (vasicine) and harmaline. Two alkaloids, harmine and peganine, were detected in the flower of P. harmala, while harmaline was only found in the ripe fruit. The total alkaloids of flower were compared with total alkaloids of ripe fruit by TLC method. Fruits and flowers had 3.12 and 3.27% alkaloid contents, respectively. Conclusion:Our results showed that the alkaloids and smoke were specifically more effective on Candida albicans and Gram- positive bacteria (Micrococcus luteus and Staphylococcus aureus), while Gram- negative bacteria, especially Pseudomonas aeruginosa, were less sensitive.

Sublethal Effects of Solanum nigrum Fruit Extract and Its Pure Glycoalkaloids on the Physiology of Tenebrio molitor (Mealworm).

Background: Solanaceae plants produce glycoalkaloids (GAs) that affect various physiological processes of herbivorous insects and they are being tested as potential alternatives for synthetic pesticides. They cause lethal and sublethal effects. Nevertheless, their mode of action remains unclear. Therefore, we examined the effects of Solanum nigrum fruit extracts and pure glycoalkaloids on a model beetle, Tenebrio molitor. Methods: Plant extracts or pure alkaloids were added to the food of the larvae for three days. The lipid, glycogen, and protein content in the fat body and the midgut were determined, and the contractility of the heart, hindgut, and oviduct muscles was tested using the video-microscopy technique. Finally, the ultrastructure of the fat body and the midgut was observed using electron microscopy. Results: No lethal effects were noted. Sublethal changes were observed in the content of biomolecules, malformations of organelles, chromatin condensation, and heart and oviduct contractility. The observed effects differed between the tested glycoalkaloids and the extract. Conclusions: Both the extract and pure GAs have a wide range of effects that may result in impaired development, food intake, and reproduction. Some early effects may be used as bioindicators of stress. The effects of the extract and pure alkaloids suggest that the substances produced by the plant may act additively or synergistically.

Glutarimide Alkaloids Through Multicomponent Reaction Chemistry

A concise four step synthetic route for glutarimide alkaloids of high biological interest is presented. The scaffold is accessed via an Ugi four component reaction, hereby introducing two points of variation. This is followed by a hydrolysis, a cyclization under mild conditions, and an amine deprotection. The diastereomers of the cyclized intermediate can be easily separated, thus leading to optically pure alkaloids. By this route, four natural products and ten derivatives were synthesized. The scope and limitations of the synthetic methodology were investigated.

Sinomenine alleviates dextran sulfate sodium‑induced colitis via the Nrf2/NQO‑1 signaling pathway.

Sinomenine (SIN), a pure alkaloid isolated from Sinomenium acutum, has been widely used in arthritis for its anti-inflammatory effect, but little is known about the effect of SIN on human ulcerative colitis (UC). In the present study, the effect and mechanism of SIN was examined in a dextran sulfate sodium (DSS)-induced murine colitis model, which mimics human UC. Oral administration of SIN significantly suppressed the elevated disease activity index and ameliorated colonic histological damage in a DSS-induced colitis model. Tumor necrosis factor-α, interleukin-6 and inducible nitric oxide synthase levels were also reduced as detected by reverse transcription-quantitative polymerase chain reaction. In addition, SIN reversed the decreased colon length and colonic superoxide dismutase activity. Furthermore, western blot analysis revealed that nuclear factor-erythroid 2-related factor 2 (Nrf2) and its downstream genes, heme oxygenase-1 and NADP(H) quinone oxidoreductase 1 (NQO-1), were markedly activated by SIN. The current results indicated that SIN alleviated DSS-induced colitis in mice, which may be due to its antioxidant properties and was at least in part dependent on the Nrf2/NQO-1 signaling pathway. Therefore, SIN may have potential applications as a protective drug for patients with UC.

Sustainable Syntheses of (\u2212)-Jerantinines A & E and Structural Characterisation of the Jerantinine-Tubulin Complex at the Colchicine Binding Site

The jerantinine family of Aspidosperma indole alkaloids from Tabernaemontana corymbosa are potent microtubule-targeting agents with broad spectrum anticancer activity. The natural supply of these precious metabolites has been significantly disrupted due to the inclusion of T. corymbosa on the endangered list of threatened species by the International Union for Conservation of Nature. This report describes the asymmetric syntheses of (−)-jerantinines A and E from sustainably sourced (−)-tabersonine, using a straight-forward and robust biomimetic approach. Biological investigations of synthetic (−)-jerantinine A, along with molecular modelling and X-ray crystallography studies of the tubulin—(−)-jerantinine B acetate complex, advocate an anticancer mode of action of the jerantinines operating via microtubule disruption resulting from binding at the colchicine site. This work lays the foundation for accessing useful quantities of enantiomerically pure jerantinine alkaloids for future development.

Isolation of Alkaloid Compounds from Ethanol Extract of Rimpang Galang Merah (Alpinia purpurata (Vielli) K. Schum) and nanoparticle production from its Alkaloid Extract. Comparative Study of Antibacterial Properties on Staphylococcus aureus and Eschericia

Isolation of alkaloid compounds from ethanol extract of red galangal rhizomes to compare their antibacterial properties with extracts formed into nanoparticles has been performed. The isolation of the alkaloid compounds was performed successively by salting using (i) hydrochloric acid (HCl) to pH 3 (when in ethanol) and using (ii) ammonium hydroxide (NH4OH) to pH 10 (when in chloroform). The alkaloid concentrated extract was obtained after chloroform was evaporated using a rotary evaporator. This alkaloid extract was then carried out by TLC silica gel GF254 using chloroform eluent: ethyl acetate (9: 2) to obtain a specific alkaloid type as a single/pure compound. Test of alkaloid purity successfully isolated by one-dimensional or two-dimensional TLC using various types of eluents with varying degrees of polarity, ie acetone, chloroform, ethyl acetate, ethanol and chloroform: ethyl acetate mixture (9: 2). Isolates of pure alkaloids were analyzed using UV-Vis spectrophotometers), FTIR and (LC-MS). The obtained alkaloid isolates were isokuinolin group with UV-visible absorption characteristics at maximum wavelength (λmaks) of 212 nm, 227 nm and 261 nm. The alkaloid isolate also showed the presence of functional groups -OH, -C-H, C = O, C = C, C = N, C-N, and C-O with molecular weight of 262.90 g/mol. The production of ethanol extract nanoparticles containing alkaloid-containing red galangal (EEAlkNPs) was performed using a top-down approach using ultrason (40 kHz, 2x50 watt) for 10 minutes. The EEAlkNPs size was determined by the dynamic light scattering method (DLS) using the Particles size analyzer (PSA) tool and obtained an average particle size of 220.2 nm. The antibacterial properties of EEAlkNPs were tested by disc diffusion method for the culture of S.aureus and E.coli bacteria. It is known that the antibacterial properties of EEAlkNPs are relatively larger than the original ethanol extract at the same concentration, ie, 2000 parts per million (bpj) or g / L.

The sinomenine enteric-coated microspheres suppressed the TLR/NF-κB signaling in DSS-induced experimental colitis

Sinomenine is a pure alkaloid with immunosuppressive effects that is extracted from the Chinese medicinal plant Sinomenium acutum. We studied the therapeutic effects of sinomenine on inflammatory bowel disease. In this study, we randomly divided mice into the following ten groups: Control group; DSS-induced colitis group; Salicylazosulfapyridine (SASP)-treated group; Chitosan-treated group; low-, medium-, and high-dose sinomenine-treated and sinomenine enteric-coated microspheres-treated groups. We recorded changes in colon length, disease activity index (DAI), and colon pathology, measured TLR4, MyD88, SIGIRR, NF-κB p65 protein levels and inflammatory serum cytokine levels.Except for the Control group, the weight of mice in each group decreased, the DAI of the DSS-induced colitis group was significantly higher than the other groups, and the DAIs of the sinomenine- and sinomenine enteric-coated microspheres-treated groups were significantly lower than that of the SASP-treated group. TLR4, MyD88, NF-κB p65 and proinflammatory cytokine expressions decreased dose dependently in the sinomenine and sinomenine enteric-coated microspheres-treated groups and were generally lower in the sinomenine enteric-coated microspheres groups. However, SIGIRR and anti-inflammatory IL-10 expressions exhibited the opposite pattern. Based on the superior therapeutic effect, sinomenine enteric-coated microspheres might regulate TLR/NF-κB signaling and would be beneficial for an effective and safe therapy of inflammatory bowel disease.

Extraits de Catharanthus roseus (cultivé in vitro et ex vitro) protecteur du foie contre la carcinogenèse chimiquement induite chez le rat Wistar

The plant extracts of Catharanthus roseus L. (G). Don has been used in alternate medicine for years, but the curing mechanism of these extracts is still not scientifically elucidated. The purpose of this study was to examine the effect of in vitro and ex vitro cultivated plant extracts in protecting the model rat against diethyl nitrosamine (DEN) induced hepatocarcinogenesis. Leaf (field and in vitro cultivated) and callus extract were administered to rats that were divided into 6 groups (G1–G6) of 6 each. The animals in each group except G1 were injected with a single dose of DEN (200 mg/kg body weight) to initiate hepatocarcinoma. Six different treatments were created; T1 (normal control), T2 (induced control); and the treated groups, T3 to T6, which received field grown or in vitro raised plant extract at various doses (300 mg/kg/day and 100 mg/kg/day). The treatments were continued for eight weeks. Biochemical variables like glutathione (GSH), glutathione S-transferase (GST), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and comparative hematology and histopathological differences were investigated. The alkaloid levels of extracts were also quantified. In carcinogenic animals, the GSH (12.80 μg/g), GST (0.38 μmol/min/mg protein), ALP (19.88 IU/L), and AST (43.61 IU/L) levels were high compared to normal. The in vitro harvested leaf extract at 100 mg/kg was the most effective treatment that reduced the levels markedly. Histological analysis confirmed the anticarcinogenic protective role of the plant extracts as treated animals showed recovery in micro fatty changes, and had reduced dilated sinusoids and localized focal degeneration. High pressure liquid chromatography (HPLC) quantified maximum accumulation of vinblastine in in vitro developed leaf extract (13.25 μg/g) compared to field grown leaf (10.72 μg/g). Vincristine content was also high (3.27 μg/g) in in vitro cultivated leaf extract. This extra accumulation of phytocompounds in extracts may be responsible for recovery from DEN caused carcinogenic damages. The protocol has advantages as preparation of extracts is easy compared to pure alkaloids, which often have toxic side effects.

Recent progress in organocatalytic asymmetric total syntheses of complex indole alkaloids

Abstract Indole and its structural analogues have been frequently found in numerous alkaloids, pharmaceutical products and related materials. The enantioselective construction of these structures allows efficient total synthesis of optically pure indole alkaloids, and hence has received worldwide interest. In the past decade, asymmetric organocatalysis has been recognized as one of the most powerful strategies to create chiral molecules with high levels of stereoselectivity. In particular, organocatalytic asymmetric cascade reactions often occur with multiple bond-breaking and forming events simultaneously or sequentially, leading to the appearance of various straightforward approaches to access core structures for asymmetric total synthesis. This review will summarize recent applications of asymmetric organocatalysis in the enantioselective synthesis of indole alkaloids.

One‐Pot N‐Deprotection and Catalytic Intramolecular Asymmetric Reductive Amination for the Synthesis of Tetrahydroisoquinolines

AbstractA one‐pot N‐Boc deprotection and catalytic intramolecular reductive amination protocol for the preparation of enantiomerically pure tetrahydroisoquinoline alkaloids is described. The iodine‐bridged dimeric iridium complexes displayed superb stereoselectivity to give tetrahydroisoquinolines, including several key pharmaceutical drug intermediates, in excellent yields under mild reaction conditions. Three additives played important roles in this reaction: Titanium(IV) isopropoxide and molecular iodine accelerated the transformation of the intermediate imine to the tetrahydroisoquinoline product; p‐toluenesulfonic acid contributed to the stereocontrol.

One-Pot N-Deprotection and Catalytic Intramolecular Asymmetric Reductive Amination for the Synthesis of Tetrahydroisoquinolines.

A one-pot N-Boc deprotection and catalytic intramolecular reductive amination protocol for the preparation of enantiomerically pure tetrahydroisoquinoline alkaloids is described. The iodine-bridged dimeric iridium complexes displayed superb stereoselectivity to give tetrahydroisoquinolines, including several key pharmaceutical drug intermediates, in excellent yields under mild reaction conditions. Three additives played important roles in this reaction: Titanium(IV) isopropoxide and molecular iodine accelerated the transformation of the intermediate imine to the tetrahydroisoquinoline product; p-toluenesulfonic acid contributed to the stereocontrol.

In vitro antibacterial effects of Zanthoxylum tingoassuiba root bark extracts and two of its alkaloids against multiresistant Staphylococcus aureus

The emergence of multiresistant strains of bacteria reinforces the need to search for new compounds able to combat resistant organisms. Medicinal plants are a great resource of bioactive substances, providing the possibility of obtaining molecules with potential antimicrobial activity. The aim of the present study is the evaluation of the antibacterial activity of extracts and alkaloids isolated from the root bark of Zanthoxylum tingoassuiba A. St.-Hil., Rutaceae, against four resistant clinical isolates and Staphylococcus aureus ATCC 25923. The dichloromethane and methanol extracts were fractionated by chromatography on silica gel, leading to the isolation of dihydrocheleryhtrine and N-methylcanadine, identified by Nuclear Magnetic Resonance spectroscopy. The antibacterial activity of the extracts and isolated compounds was evaluated by the disc diffusion method and the minimum inhibitory concentration was determined. The dichloromethane extract was the most active against all the tested strains and the two pure alkaloids were more active than the extracts. The anti-MRSA activity of the two benzophenanthridine alkaloids is demonstrated for the first time in this study. These compounds appear as potential leads for the development of new anti-MRSA compounds and could be responsible for the antibacterial activity, justifying the ethnobotanical use of Z. tingoassuiba and other species for the treatment of various infectious diseases.

Dermal wound healing potency of single alkaloid (betaine) versus standardized crude alkaloid enriched-ointment of Evolvulus alsinoides

Context: Evolvulus alsinoides Linn. (Convolvulaceae), well known as shankhpushpi in Ayurvedic text, is traditionally used for several healing purposes.Objective: A comparative evaluation of dermal wound healing potential of acidic and basic alkaloid enriched-ointment (AAO and BAO) of aerial parts of E. alsinoides versus pure alkaloid, betaine (BEO), was undertaken.Material and methods: The effect of topical application (50 mg/animal/day) of AAO-1%, AAO-2%, BAO-1%, BAO-2%, BEO-0.5% and BEO-1% was assessed through excision (14 days) and incision (10 days) models on rats. The percentage wound contraction, total protein content, and breaking strengths were determined followed by histopathological studies.Results and discussion: The total alkaloid in acidic and basic alkaloid enriched fractions was found to be 0.1114 and 0.1134 μg/mL, respectively. Thus, 0.1528, 0.3056, 0.1380 and 0.2459 μg of total alkaloid were estimated to be present in AAO-1%, AAO-2%, BAO-1% and BAO-2%, respectively. AAO and BAO promoted wound healing activity significantly in both the models. Higher rate of wound contraction (p < 0.001) with significant increase in protein content in the treatment groups (from 2.32 to 2.55) demonstrated stimulation of cellular proliferation and epithelization, which was further supported by histopathological reports. High skin breaking strength (mean value 393 in control was increased to the range of 535–572 in treated groups) proved a significant (p < 0.001) wound healing potential of E. alsinoides. Early dermal and epidermal regeneration in drug-treated groups also confirmed the positive effect.Conclusion: Observation of higher healing power of alkaloid enriched-ointment compared with single alkaloid ointment corroborated the synergy mechanism.