Purdue Improved Crop Storage Research Articles

Triple bag hermetic technology for controlling a bruchid (Spermophagus sp.) (Coleoptera, Chrysomelidae) in stored Hibiscus sabdariffa grain

We assessed the performance of hermetic triple layer Purdue Improved Crop Storage (PICS) bags for protecting Hibiscus sabdariffa grain against storage insects. The major storage pest in the grain was a bruchid, Spermophagus sp.. When we stored infested H. sabdariffa grain for six months in the woven polypropylene bags typically used by farmers, the Spermophagus population increased 33-fold over that initially present. The mean number of emergence holes per 100 seeds increased from 3.3 holes to 35.4 holes during this time period, while grain held for the same length of time in PICS bags experienced no increase in the numbers of holes. Grain weight loss in the woven control bags was 8.6% while no weight loss was observed in the PICS bags. Seed germination rates of grain held in woven bags for six months dropped significantly while germination of grain held in PICS bags did not change from the initial value. PICS bags can be used to safely store Hibiscus grain after harvest to protect against a major insect pest.

Triple bagging hermetic technology for post-harvest preservation of paddy rice Oryza sativa L. in the Sahel of West Africa

Trials of the Purdue Improved Crop Storage (PICS) bag technology for the storage of paddy rice, Oryza sativa L., were conducted in Burkina Faso, Ghana and Niger. Paddy rice naturally infested with insects, the most abundant species being Tribolium spp. and Rhizopertha dominica, was sealed in triple layer PICS bags, or in conventional woven polypropylene sacks. At the end of 7–18 months of storage in PICS bags the number of insects did not increase, the weight of 100 seeds did not change, and the proportion of damaged seeds was not different from that present when the paddy was first put into the bags. By contrast, paddy stored in the conventional way in woven polypropylene bags exhibited weight losses ranging from 3 to 8.7%. In Bolgatanga, Ghana, where paddy was stored for 18 months, germination of paddy kept in the PICS bags was comparable to that present at the beginning of the experiment. PICS bags can be used for the safe, low-cost, insecticide free storage of paddy rice.

Low permeability triple-layer plastic bags prevent losses of maize caused by insects in rural on-farm stores

Participatory on-farm trials were conducted to assess effectiveness of Purdue Improved Crop Storage (PICS™) bags for storage of maize in small-scale farmers’ stores in rural villages in eastern Kenya. A PICS bag is a three-layered hermetic bag-system that forms a barrier against the influx of oxygen and the escape of carbon dioxide. Jute, woven polypropylene or PICS bags were filled with shelled maize grain, purchased from the participating farmers, and the three sets of bags kept in the farmers’ own stores for 35 weeks. Oxygen and carbon dioxide levels in the PICS bags were monitored, as well as the temperature and relative humidity in all the bags. Grain moisture, live insect population, grain damage and weight loss were examined at intervals of seven weeks. Oxygen and carbon dioxide composition demonstrated that PICS bags are capable of sustaining good air-barrier properties under farmer storage conditions. Moreover, moisture content of maize stored in PICS bags did not change throughout the storage period whereas the moisture content of maize stored in polypropylene and jute bags decreased significantly in the final 14 weeks. Maize stored in PICS bags remained free from insect infestation and the weight loss due to insect damage was below 1 %. On the contrary, polypropylene and jute bags permitted profuse build-up of insect populations. At 35 weeks, grain damage reached 77.6 % and 82.3 % corresponding to 41.2 % and 48.5 % weight loss in the polypropylene and jute bags respectively. These findings demonstrate that PICS bags are effective in controlling losses caused by storage pests under farmer storage conditions.

PICS bags protect wheat grain, Triticum aestivum (L.), against rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

Purdue Improved Crop Storage Bags (PICS) bags are hermetic containers consisting of two inner layers of high-density polyethylene (HDPE) surrounded by an outer woven polypropylene bag. They have proven effective in preventing losses of stored cowpea grain to the cowpea bruchid, Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae) and perform well with other dry grains attacked by several species of postharvest pests. We tested PICS bags against the rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae), a common stored grain pest. Storing rice weevil infested wheat in PICS bags arrested weevil population growth and preserved the grain well. The air surrounding infested wheat held in PICS bags had lower oxygen and higher carbon dioxide levels compared to the ambient atmosphere. Seed germination declined over time in infested PICS bags, but less so than did grain held in infested woven bags. Low-cost, durable, pesticide-free PICS technology offers an inexpensive, practical and useful way to protect wheat against rice weevil.

Purdue Improved Crop Storage (PICS) bags for safe storage of groundnuts

Groundnut seeds are prone to quality deterioration and damage due to improper storage. Hermetic storage of pods offers a novel, sustainable and ecologically safe alternative over traditional methods. In this paper, we demonstrate the efficacy of triple-layer “Purdue Improved Crop Storage (PICS)” bags, (that comprises of two inner high density polyethylene bags and one outer woven polypropylene bag), for protecting pods from quality deterioration, damage by bruchids (Caryedon serratus) and aflatoxin contamination (Aspergillus flavus). Custom made triple-layer bags were used and pods (of cv ICGV 91114) were placed @ 2 kg/bag. Over four months of storage under ambient conditions, triple-layer bags supported retention of seed weight, germinability and oil content significantly better than cloth bags. Further, under both natural and artificial infestations with A. flavus, seed aflatoxins levels were lower in PICS bags compared to cloth bags. Toxin accumulation in PICS bags deliberately infested with bruchids and A. flavus was less compared to cloth bags under similar conditions. Bruchid damage to pods was less in PICS bags versus cloth bags in all cases. Our results suggest the superiority of triple-layer PICS bags over cloth bags in protecting seed viability, seed weight and oil content while safeguarding the groundnuts from bruchids and retarding toxin accumulation.

Assessing Purdue Improved Crop Storage (PICS) bags to mitigate fungal growth and aflatoxin contamination

Storing maize in regions of the world without sufficient drying and storage capacity is challenging due to the potential risk of aflatoxin contamination produced by Aspergillus flavus. This study sought to determine if storage of maize in Purdue Improved Crop Storage (PICS) bags prevents mold growth and aflatoxin accumulation. PICS bags are a three-layer, hermitic bag-system that forms a barrier against the influx of oxygen and the escape of carbon dioxide. Maize conditioned at 12, 15, 18, and 21% grain moisture was inoculated with 50 g of maize kernels infected with fluorescent-marked strain of A. flavus. The grain was stored in either PICS or woven bags at 26 °C, and percent oxygen/carbon dioxide levels, fungal growth, aflatoxin, moisture content, and kernel germination were assessed after 1 and 2 months incubation. Maize stored in woven bags was found to equilibrate with the ambient moisture environment over both storage periods, while PICS bags retained their original moisture levels. Aspergillus flavus growth and aflatoxin accumulation were not observed in maize stored in any PICS bags. No aflatoxin B1 was detected in woven bags containing low-moisture maize (12 and 15%), but detectable levels of aflatoxin were observed in high moisture maize (18 and 21%). The percentage of oxygen and carbon dioxide within PICS bags were dependent on initial grain moisture. Higher carbon dioxide levels were observed in the bags stored for 1 month than for 2 months. High initial moisture and carbon dioxide levels correlated with low kernel germination, with the 18 and 21% treatment groups having no seeds germinate. The results of the study demonstrate that storage of maize in PICS bags is a viable management tool for preventing aflatoxin accumulation in storage.

Storage of mung bean (Vigna radiata [L.] Wilczek) and pigeonpea grains (Cajanus cajan [L.] Millsp) in hermetic triple-layer bags stops losses caused by Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)

Dry mung bean and pigeonpea grains that had sustained some insect damage but fumigated before the start of the experiment were stored in triple-layer hermetic bags (Purdue Improved Crop Storage (PICS™ bags) or woven polypropylene (PP) bags for 6 months. Some of the bags were artificially infested with cowpea bruchid Callosobruchus maculatus (F.) (PICS1, PP1) while others were not (PICS0, PP0). In an additional trial, PP bags containing the grains were treated with Actellic Super® dust before being artificially infested (PP1Ac). Moisture content, live adult C. maculatus count, grain damage, weight loss, and seed germination were determined on a monthly basis. At six months, moisture contents of grain stored in PICS and PP bags remained below 12%. Storage in PICS bags halted multiplication of C. maculatus, and the initial damage level and weight of grains did not change. Conversely, in the PP bags, C. maculatus populations increased massively and seed damage reached 71.8 ± 1.9%, 76.9 ± 0.4%, and 60.3 ± 0.6% corresponding to weight losses of 14.5 ± 0.1%, 16.5 ± 0.2% and 12.5 ± 0.1% in PP0, PP1 and PP1Ac, respectively, in mung beans. With the pigeonpeas, seed damage reached 55.1 ± 0.6%, 95.7 ± 0.4% and 75.8 ± 0.9%, corresponding to weight losses of 13.0% ± 0.3%, 26.2 ± 0.2% and 13.5 ± 0.1%, in PP0, PP1 and PP1Ac, respectively. PICS bags are an effective tool for preserving mung beans and pigeonpeas against C. maculatus attack, and their performance is superior to that of Actellic Super® dust.

PICS bags for post-harvest storage of maize grain in West Africa

Maize is a key food crop in West Africa, where Benin, Ghana, Burkina Faso and Nigeria account for most of the regional production. Storage pests are major constraints in the maize value chain, with losses reaching 30%. Simple, low-cost triple bagging technology (now known as PICS – Purdue Improved Crop Storage) originally developed for postharvest storage of cowpea was evaluated for applicability to maize storage. Thirteen test units employing PICS bag were set up in July and August 2012 at eleven localities in Benin, Burkina Faso and Ghana. Each trial unit consisted of 3 or 4 PICS bags of 50 kg capacity and 2 woven control bags. We used locally purchased naturally infested maize that had relatively high levels of infestation with Prostephanus truncatus Horn, Sitophilus zeamais Motschulsky, 1855 and/or Rhyzopertha dominica (F.) (seven localities) or lightly infested maize showing little or no evidence of damage (four localities). After 6.5 months of storage in PICS bags, there was 95–100% insect mortality at all sites. The percentage of damaged seeds and the 100 seeds weights in PICS bags were unchanged from what was recorded at the time the experiment was set up. Seed viability was well maintained; germination rates of seeds from the PICS bags did not differ from that observed at the beginning of the experiment. Aflatoxin tests were carried out on 245 samples, with 53% having levels above 20 ppm in both PICS and woven bags. Samples from PICS bags tended to be less contaminated than those from woven bags. PICS bags can be used for maize storage even in areas with high prevalence of P. truncatus, but storage of maize should begin soon after harvest and drying to minimize bag damage that can occur when very high numbers of P. truncatus are present.

Adoption of cowpea hermetic storage by women in Nigeria, Niger and Burkina Faso

In this study, 2741 randomly selected rural women were interviewed about their cowpea storage practices in 101 villages in Burkina Faso, Niger and Nigeria in late 2010 and early 2011. The overall objective was to determine their cowpea storage practices and identify the most important factors in choosing Purdue Improved Crop Storage (PICS) triple bag storage. About two thirds of women said they used some type of hermetic storage. The hermetic containers included metal drums, plastic jugs, double bags and triple bags. The weighted percentage of women using PICS triple layer bags is 46%. Quantity of cowpea stored by technology showed similar patterns. Overall the percentage of cowpea in hermetic storage was 64%. The study estimated that women stored 50% of their cowpea in PICS bags. The percentage of cowpea in hermetic storage overall and in PICS bags specifically is higher for women than for men in a parallel 2012 ten-country study of mostly male household heads. In PICS villages, the women cite PICS technicians as the most important source of information. In Non-PICS villages, radio was the most important. Most women say that higher income is the major benefit of PICS. The 2009–2010 three country weighted average of the net cash flow from cowpea storage in PICS bags is $10.81/100 kg bag and $39.27 per respondent. Overall, the women indicated that local unavailability was the primary constraint to use of PICS bags. The LOGIT regression analysis shows that the most important factor influencing use of PICS technology is living in a village where PICS demonstrations occurred. The regression shows that radio and the PICS technicians have key roles as information sources. Being able to attend mixed gender meetings was statistically significant only in Burkina Faso where PICS did not organize many women-only PICS activities.

Triple bag hermetic technology for post-harvest preservation of Bambara groundnut (Vigna subterranea (L.) Verdc.)

Experiments were conducted to evaluate the performance of hermetic triple bagging using Purdue Improved Crop Storage (PICS) bags for storage of Bambara groundnut (Vigna subterranea (L.) Verdc.). One set of experiments used grain heavily infested by Callosobruchus maculatus (F.) while a second set began with a low level infestation. Each experiment consisted of V. subterranea grain kept in four replicate 50 kg PICS bags or two replicate woven bags as controls. Two to five days after the beginning of the experiments, oxygen level inside the bags averaged about 21% (v/v) in the controls but decreased significantly in PICS bags, reaching 10% (v/v) with the heavily infested grain but falling only slightly in the lightly infested grain. After 7 months of storage, (i) the number of C. maculatus adults found between and within the grains, (ii) the 100 seed weight, (iii) the number of seed with holes, and (iv) the percentage germination of grain stored in PICS bags did not differ from what had been measured on the day that the experiment was set up. In woven bags, by contrast, there was a massive increase in C. maculatus numbers with means of 309 and 251 adults per 500 g in heavily and lightly infested grain, respectively. Grain weight losses in the woven bag controls ranged from 8 to 19% and the percentage of C. maculatus emergence holes per 100 seed increased from 51 to 135%.

A simple methodology for measuring profitability of on-farm storage pest management in developing countries

We present a simple financial model for storage researchers to measure the profitability of storage protection for marketing producers in developing countries. We examine the relationship between the value of a stored commodity and required price seasonality for profitable storage under a range of possible fixed costs of storage and opportunity costs of capital. The cost of storage protection has a larger effect on storage profitability with low value commodities such as maize, while the opportunity cost of capital has a larger effect on storage profitability of high value commodities such as cowpeas and common beans. An example is drawn with maize in Malawi, contrasting the profitability of storage protection with hermetic Purdue Improved Crop Storage (PICS) bags versus government-subsidized chemical protectants. Results from this example show that while PICS bags financially outperform chemical protectants, profitability varies greatly both by year and region of the country. We additionally include a Microsoft Excel template and interactive website link along with an explanation of the financial model to facilitate incorporation of economics in storage research on insect losses and technology adoption.