Pupil Area Research Articles

Pupillary stress index: A new thermal comfort index for crossbred goats

ABSTRACT Goats are homeothermic animals considered as rustic from the bioclimatic point of view, but the reduction of their productive efficiency may occur in unfavorable thermal conditions. The present study was carried out to evaluate pupil dilation as an indicator of thermal stress in Boer crossbred goats maintained under controlled environmental conditions. Six male crossbred goats of the Boer breed were distributed in a completely randomized design submitted to temperatures of 26, 29 and 33 °C. The pupillary dilation and pupillary temperature, respiratory frequency, heart rate, surface and rectal temperatures were monitored. The significant increase in physiological responses occurred in response to the increase in air temperature. An increase of pupillary dilation was observed in the animals with the elevation of the air temperature, with an increase of 26.96 mm2 in pupillary area when comparing the temperatures 26 and 33 °C, and it was possible to establish a correlation between air temperature and pupillary diameter increase, named pupillary stress ratio (PSR).

Signal and noise in helioseismic holography

Context. Helioseismic holography is an imaging technique used to study heterogeneities and flows in the solar interior from observations of solar oscillations at the surface. Holographic images contain noise due to the stochastic nature of solar oscillations. Aims. We aim to provide a theoretical framework for modeling signal and noise in Porter–Bojarski helioseismic holography. Methods. The wave equation may be recast into a Helmholtz-like equation, so as to connect with the acoustics literature and define the holography Green’s function in a meaningful way. Sources of wave excitation are assumed to be stationary, horizontally homogeneous, and spatially uncorrelated. Using the first Born approximation we calculated holographic images in the presence of perturbations in sound-speed, density, flows, and source covariance, as well as the noise level as a function of position. This work is a direct extension of the methods used in time-distance helioseismology to model signal and noise. Results. To illustrate the theory, we compute the holographic image intensity numerically for a buried sound-speed perturbation at different depths in the solar interior. The reference Green’s function is obtained for a spherically-symmetric solar model using a finite-element solver in the frequency domain. Below the pupil area on the surface, we find that the spatial resolution of the holographic image intensity is very close to half the local wavelength. For a sound-speed perturbation of size comparable to the local spatial resolution, the signal-to-noise ratio is approximately constant with depth. Averaging the image intensity over a number N of frequencies above 3 mHz increases the signal-to-noise ratio by a factor nearly equal to the square root of N. This may not be the case at lower frequencies, where large variations in the holographic signal are due to the contributions from the long-lived modes of oscillation.

Reward elicits cognitive control over emotional distraction: Evidence from pupillometry

Attention is biased toward emotional stimuli, even when they are irrelevant to current goals. Motivation, elicited by performance-contingent reward, reduces behavioural emotional distraction. In emotionally neutral contexts, reward is thought to encourage use of a proactive cognitive control strategy, altering anticipatory attentional settings to more effectively suppress distractors. The current preregistered study investigates whether a similar proactive shift occurs even when distractors are highly arousing emotional images. We monitored pupil area, an online measure of both cognitive and emotional processing, to examine how reward influences the time course of control. Participants (n = 110) identified a target letter flanking an irrelevant central image. Images were meaningless scrambles on 75% of trials; on the remaining 25%, they were intact positive (erotic), negative (mutilation), or neutral images. Half the participants received financial rewards for fast and accurate performance, while the other half received no performance-contingent reward. Emotional distraction was greater than neutral distraction, and both were attenuated by reward. Consistent with behavioural findings, pupil dilation was greater following emotional than neutral distractors, and dilation to intact distractors (regardless of valence) was decreased by reward. Although reward did not enhance tonic pupil dilation (an index of sustained proactive control), exploratory analyses showed that reward altered the time course of control-eliciting a sharp, rapid, increase in dilation immediately preceding stimulus onset (reflecting dynamic use of anticipatory control), that extended until well after stimulus offset. These findings suggest that reward alters the time course of control by encouraging proactive preparation to rapidly disengage from emotional distractors.

Patterns of Pupillary Activity During Binocular Disparity Resolution.

This study examined the dynamic coordination between disconjugate, vergence eye movements, and pupil size in 52 normal subjects during binocular disparity stimulation in a virtual reality display. Eye movements and pupil area were sampled with a video-oculographic system at 100 Hz during performance of two tasks, (1) fusion of a binocular disparity step (±1.5° of visual angle in the horizontal plane) and (2) pursuit of a sinusoidally varying binocular disparity stimulus (0.1 Hz, ±2.6° of visual angle in the horizontal plane). Pupil size data were normalized on the basis of responses to homogeneous illumination increments ranging from 0.42 to 65.4 cd/m2. The subjects produced robust vergence eye movements in response to disparity step shifts and high fidelity sinusoidal vergence responses (R2 relative to stimulus profile: 0.933 ± 0.088), accompanied by changes in pupil area. Trajectory plots of pupil area as a function of vergence angle showed that the pupil area at zero vergence is altered between epochs of linear vergence angle—pupil area relations. Analysis with a modified Gath-Geva clustering algorithm revealed that the dynamic relationship between the ocular vergence angle and pupil size includes two different transient, synkinetic response patterns. The near response pattern, pupil constriction during convergence and pupil dilation during divergence, occurred ~80% of the time across subjects. An opposite, previously undescribed synkinetic pattern was pupil constriction during divergence and pupil dilatation during convergence; it occurred ~15% of the time across subjects. The remainder of the data were epochs of uncorrelated activity. The pupil size intercepts of the synkinetic segments, representing pupil size at initial tropia, had different relationships to vergence angle for the two main coordinated movement types. Hippus-like movements of the pupil could also be accompanied by vergence movements. No pupil coordination was observed during a conjugate pursuit task. In terms of the current dual interaction control model (1), findings suggest that the synkinetic eye and pupillary movements are produced by a dynamic switch of the influence of vergence related information to pupil control, accompanied by a resetting of the pupil aperture size at zero-vergence.

Graft rejection-like reactions in the early postoperative period after deep anterior lamellar keratoplasty for keratoconus: a retrospective study.

BackgroundDeep anterior lamellar keratoplasty (DALK) is indicated to correct high astigmatism in patients with keratoconus (KC) and no evidence of Descemet’s membrane rupture. However, some patients with KC experience graft rejection-like inflammatory reactions within 2 months (usually in the first week) after DALK. The aim of this study was to identify the characteristics and influencing factors of these reactions by reviewing the records of patients who underwent DALK or penetrating keratoplasty (PKP) for KC or other corneal problems.MethodsWe retrospectively reviewed the medical records of patients who underwent DALK for KC (DALK/KC), PKP for KC (PKP/KC), or DALK for other corneal problems (DALK/non-KC) at Ban Buntane Hotokukai Hospital between January 2006 and December 2015 and who were followed for more than 1 year. We collected data on the characteristics and incidence of severe inflammatory graft reactions in the early postoperative phase (ie, within 2 months after keratoplasty) and visual outcomes after these inflammatory reactions.ResultsPostoperative inflammatory reactions characterized by persistent epithelial defects, loose suture with infiltration, and vessel invasion occurred in seven of eleven DALK/KC patients, three of 50 DALK/non-KC patients, and none of five PKP/KC patients. These reactions were nonresponsive to topical steroids, and suture removal was required. Although a clear corneal graft in the pupillary area was obtained and best-corrected visual acuity was good after the resolution of inflammation, a risk of corneal astigmatism remained.ConclusionThe incidence of these reactions in the early postoperative period is high after DALK for KC. These reactions do not respond well to topical steroids, and suture removal may be required, which may cause high astigmatism after the inflammation subsides. Lamellar keratoplasty should be considered carefully for patients with KC.

Effects of corneal thickness distribution and apex position on postoperative refractive status after full-bed deep anterior lamellar keratoplasty.

To investigate the effects of corneal thickness distribution and apex position on postoperative refractive status after full-bed deep anterior lamellar keratoplasty (FBDALK). This is a retrospective analysis of patients who were diagnosed with advanced keratoconus between 2011 and 2014 in our hospital. The base of the cone in all patients did not exceed the central cornea at a 6-mm range. The FBDALK was performed by a same surgeon. All patients had a complete corneal suture removal and the follow-up records were intact. Patients who had graft-bed misalignment or who were complicated with a cataract or glaucoma were excluded. Uncorrected visual acuity (UCVA), best spectacle corrected visual acuity (BSCVA), and Pentacam examination data were recorded at two years postoperatively. The recorded data included the superior-inferior (S-I) and nasal-temporal (N-T) corneal thickness differences in 2, 4, 6, and 8 mm diameter concentric circles with the corneal apex as the center (S-I2 mm, S-I4 mm, S-I6 mm, S-I8 mm, N-T2 mm, N-T4 mm, N-T6 mm, and N-T8 mm), the linear, X-axis, and Y-axis distance between the corneal pupillary center and the cornea apex, total corneal astigmatism at a zone of 3 mm diameter from the corneal apex (TA3 mm), the astigmatic vector values J0 and J45, and the corneal total higher-order aberration for 3 and 6 mm pupil diameters (HOA3 mm and HOA6 mm). Statistical analysis was performed by SPSS 15.0. A total of 47 eyes of 46 patients met the criteria and were included in this study. The mean follow-up time was (28±7) months. The mean UCVA was 0.45±0.23 (logMAR) (MAR: minimum angle of resolution) and the mean BSCVA was 0.19±0.15 (logMAR), which were all significantly positively correlated with postoperative TA3 mm and HOA3 mm. The mean S-I corneal thickness differences were (44.62±37.74) μm, and the mean N-T was (38.57±32.29) μm. S-I2 mm was significantly positively correlated with J0 (r=0.31), J45 (r=0.42), HOA3 mm (r=0.37), and HOA6 mm (r=0.48). S-I4 mm and S-I8 mm were significantly positively correlated with HOA3 mm (r=0.30, r=0.40) and HOA6 mm (r=0.46, r=0.35). The X-axis distance between corneal pupillary center and corneal apex was significantly positively correlated with J45 (r=0.29). In patients with advanced keratoconus after FBDALK, the unevenly distributed thickness at corneal pupillary area and the misalignment of corneal apex and pupillary center might cause significant regular and irregular astigmatism, which affected the postoperative visual quality.

Pupil Detection Based on Color Difference and Circular Hough Transfor

<span>Human pupil eye detection is a significant stage in iris segmentation which is representing one of the most important steps in iris recognition. In this paper, we present a new method of highly accurate pupil detection. This method is consisting of many steps to detect the boundary of the pupil. First, the read eye image (R, G, B), then determine the work area which is consist of many steps to detect the boundary of the pupil. The determination of the work area contains many circles which are larger than pupil region. The work area is necessary to determine pupil region and neighborhood regions afterward the difference in color and intensity between pupil region and surrounding area is utilized, where the pupil region has color and intensity less than surrounding area. After the process of detecting pupil region many steps on the resulting image is applied in order to concentrate the pupil region and delete the others regions by using many methods such as dilation, erosion, canny filter, circle hough transforms to detect pupil region as well as apply optimization to choose the best circle that represents the pupil area. The proposed method is applied for images from palacky university, it achieves to 100 % accurac</span>

Are there facial indicators of positive emotions in birds? A first exploration in Japanese quail

The positive aspect of emotions, like pleasure, remains overlooked in birds. Our aim was to contribute to the exploration of facial indicators of positive emotions. To observe contrasting emotional expressions, we used two lines of Japanese quail divergently selected on their inherent fearfulness: a fearful line (long tonic immobility duration: LTI) and a less fearful line (short tonic immobility duration: STI). To induce positive emotions, we gave individual quail the opportunity to perform a rewarding behaviour, dustbathing, in an unfamiliar cage. More STI than LTI quail expressed dustbathing and latencies to dustbathe were significantly shorter in STI than LTI quail. This result indicated that the lines of quail differed in their fearfulness of the situation. We observed crown feather height, throat feather angle and pupil surface before (control) and during dustbathing. We found significant increases in crown feather height, pupil area and angle of throat feathers between the control and the dustbathing phases in STI quail, and pupil area correlated positively with crown feather height. In LTI quail, the angle of throat feathers increased during dustbathing, but the other parameters did not differ. We argue that variation in crown feather height and pupil area may provide indications of positive emotions in Japanese quail.

Ocular Effects of Exposure to 40, 75, and 95\xa0GHz Millimeter Waves

The objective of this study was to develop a model of ocular damage induced by 40, 75, and 95 GHz continuous millimeter waves (MMW), thereby allowing assessment of the clinical course of ocular damage resulting from exposure to thermal damage-inducing MMW. This study also examined the dependence of ocular damage on incident power density. Pigmented rabbit eyes were exposed to 40, 75, and 95 GHz MMW from a spot-focus-type lens antenna. Slight ocular damage was observed 10 min after MMW exposure, including reduced cornea thickness and reduced transparency. Diffuse fluorescein staining around the pupillary area indicated corneal epithelial injury. Slit-lamp examination 1 day after MMW exposure revealed a round area of opacity, accompanied by fluorescence staining, in the central pupillary zone. Corneal edema, indicative of corneal stromal damage, peaked 1 day after MMW exposure, with thickness gradually subsiding to normal. Three days after exposure, ocular conditions had almost normalized, though corneal thickness was slightly greater than that before exposure. The 50% probability of ocular damage (DD50) was in the order 40 > 95 ≈ 75 GHz at the same incident power densities.

The pupillary light responses of animals; a review of their distribution, dynamics, mechanisms and functions.

The timecourse and extent of changes in pupil area in response to light are reviewed in all classes of vertebrate and cephalopods. Although the speed and extent of these responses vary, most species, except the majority of teleost fish, show extensive changes in pupil area related to light exposure. The neuromuscular pathways underlying light-evoked pupil constriction are described and found to be relatively conserved, although the precise autonomic mechanisms differ somewhat between species. In mammals, illumination of only one eye is known to cause constriction in the unilluminated pupil. Such consensual responses occur widely in other animals too, and their function and relation to decussation of the visual pathway is considered. Intrinsic photosensitivity of the iris muscles has long been known in amphibia, but is in fact widespread in other animals. The functions of changes in pupil area are considered. In the majority of species, changes in pupil area serve to balance the conflicting demands of high spatial acuity and increased sensitivity in different light levels. In the few teleosts in which pupil movements occur they do not serve a visual function but play a role in camouflaging the eye of bottom-dwelling species. The occurrence and functions of the light-independent changes in pupil size displayed by many animals are also considered. Finally, the significance of the variations in pupil shape, ranging from circular to various orientations of slits, ovals, and other shapes, is discussed.

Влияние ортокератологических линз на сферическую аберрацию оптической системы глаза

The purpose of this study is to measure the spherical aberration (SA) of the cornea and the optical system of the eye before and after wearing orthokeratologic (OK) contact lenses. Material and methods. 22 patients (44 eyes) with myopia aged 11 to 18 years were examined. The total SA was determined after medical cycloplegia performed on OPD-Scan III aberrometer, Nidek. To analyze SA values before and after wearing OK lenses, we chose pupil areas from 3 mm to 7.5 mm in 0.5-1 mm increments. Results. In intact eyes with myopia, the total SA is mildly positive and is growing with the increase of the pupil diameter. After OK correction, the SA is changing: from 3 to 6 mm of the pupillary area, the positive SA increases by 50-130 %, in the 6.3 mm area it falls sharply and becomes negative, growing on the way to the periphery of the pupillary area. Conclusions. Corneal topography changes after OK corrections lead to specific changes of the wavefront of myopic eyes, causing increased visual performance and eliminating the hyperopic defocus at retinal periphery. For citation: Tarutta E.P., Harutyunyan S.G. The impact of orthokeratologic contact lenses on spherical aberration of the optical system of the eye. Russian ophthalmological journal. 2018; 11 (2): 17-21. doi: 10.21516/2072-0076-2018-11-2-17-21 (In Russian).

Receptive Field Mechanism and Pupilary Light Reflex for the Assessment of Visual Discomfort

Discomfort glare is defined as glare that causes discomfort without necessarily impairing the vision of objects. Traditional glare metrics fail for non-uniform luminaires. As an alternative, visual discomfort is determined by a physiological model incorporating the centre­surround receptive field mechanism and the pupillary light reflex. The pupil area, controlled by the pupillary light reflex, regulates the retinal illuminance. A centre-surround receptive field, described by a difference of Gaussians, represents the visual signal. The centre excites the signal whereas the surround controls the inhibition. A forced choice paired comparison experiment involves 7 non-uniform rear projected stimuli with different spatial frequencies. Inspired by a promising coefficient of determination of 0.90, the model is a candidate to replace current glare metrics as UGR or VCP, especially when non­uniform luminaires are to be evaluated.

Real-time eye tracking for the assessment of driver fatigue.

Eye-tracking is an important approach to collect evidence regarding some participants’ driving fatigue. In this contribution, the authors present a non-intrusive system for evaluating driver fatigue by tracking eye movement behaviours. A real-time eye-tracker was used to monitor participants’ eye state for collecting eye-movement data. These data are useful to get insights into assessing participants’ fatigue state during monotonous driving. Ten healthy subjects performed continuous simulated driving for 1–2 h with eye state monitoring on a driving simulator in this study, and these measured features of the fixation time and the pupil area were recorded via using eye movement tracking device. For achieving a good cost-performance ratio and fast computation time, the fuzzy K-nearest neighbour was employed to evaluate and analyse the influence of different participants on the variations in the fixation duration and pupil area of drivers. The findings of this study indicated that there are significant differences in domain value distribution of the pupil area under the condition with normal and fatigue driving state. Result also suggests that the recognition accuracy by jackknife validation reaches to about 89% in average, implying that show a significant potential of real-time applicability of the proposed approach and is capable of detecting driver fatigue.

Analysing the impact of myopia on the Stiles-Crawford effect of the first kind using a digital micromirror device.

Photoreceptor light acceptance is closely tied to the Stiles-Crawford effect of the first kind (SCE-I). Whether the SCE-I plays a role in myopic development remains unclear although a reduction in directionality has been predicted for high myopia. The purpose of this study is to analyse the relationship between foveal SCE-I directionality, axial eye length, and defocus for emmetropic subjects wearing ophthalmic trial lenses during psychophysical measurements and for myopic subjects with their natural correction. A novel uniaxial flicker system has been implemented making use of a Digital Micromirror Device (DMD) to flicker between a 2.3 visual degrees circular reference and a set of circular test patterns in a monocular Maxwellian view at 0.5 Hz. The brightness of the test is adjusted by the duty cycle of the projected light to an upper limit of 22727Hz. The wavelength and bandwidth are set by a tuneable liquid-crystal filter centred at 550 nm. A total of four measurement series for 11 pupil entrance points have been realized for the right eye of 6 emmetropic and 10 myopic subjects whose pupils were dilated with tropicamide. Five of the emmetropic subjects wore ophthalmic trial lenses in the range of -3 to +9 dioptres to mimic hyperopic to highly myopic vision and resulting visibility plots have been fitted to a Gaussian SCE-I function. In turn, the myopic subjects wore their natural correction during the analysis of the SCE-I. All subjects had their axial eye length determined with an ultrasound device. A SCE-I directionality parameter in the range of 0.03 to 0.06/mm2 was found for the emmetropic subjects with corrected vision in fair agreement to values in the literature. The results also revealed a marked reduction in directionality in the range from 16% to 30% with every 3 dioptre increase of simulated myopia, as well as a 10% increased directionality in simulated hyperopic eyes. For both emmetropic and myopic subjects, a decrease in directionality with increase in axial length was found in agreement with theoretical expectations. The study confirms a clear link between SCE-I directionality, uncorrected defocus, and axial eye length. This may play a role for emmetropization and thus myopic progression as cone photoreceptors capture light from a wider pupil area in elongated eyes due to a geometrical scaling.

Femtosecond Laser-Assisted Ipsilateral Rotational Lamellar Autokeratoplasty.

To describe the surgical technique and report the outcomes of 2 patients treated with femtosecond laser-assisted ipsilateral rotational lamellar autokeratoplasty in central corneal scars. The corneal scar depth was mapped using preoperative optical coherence tomography. An eccentric lamellar lenticule was cut with a femtosecond laser and rotated to decenter corneal opacity and free the pupil area in 2 patients with nonprogressive central corneal scars. The surgical plan was set after simulating lenticule rotation with a digital corneal image and computer software. In both cases, the corneal scar was decentered inferiorly, out of the pupillary area, with increased postoperative visual acuity but visual outcome limitations secondary to corneal irregularities and residual deep opacity. Femtosecond laser-assisted ipsilateral rotational lamellar autokeratoplasty is effective for shifting central corneal opacities and can be considered in appropriate cases.

Auto-brightness control technology depending on user’s pupil area

Auto-brightness control technology depending on user’s pupil area

FEATURES OF THE PHACOEMULSIFICATION TECHNIQUE OF CATARACT IN PSEUDOEXFOLIATION SYNDROME

Traditional surgery of cataract in pseudoexfoliation syndrome (PEX) can be excessively aggressive and increase zonular instability. The main technological target of this surgery is reducing of a zonular stress at all stages and especially when removing the lens nucleus. Purpose was to develop an effective and safe technique for nucleus removal without rotating crystalline lens during phacoemulsification in PEX. Material and methods. 765 eyes with PEX were treated by phacoemulsification without nuclear rotation using original technique. The left paracentral oblique groove was made after capsulotomy and hydrodissection. Nucleus was divided by phaco tip and chopper. In right heminucleus a lateral deep groove was performed such way to create a big asymmetric wedge including central part of nucleus. This wedge was removed without rude manipulation and rotation. The remaining two peripheral segments were easily gripped and pull into pupil area for safe phacoemulsification. Results. In all cases this technique allowed for safe removing of the nucleus. Cataract surgery was finished without serious intraoperative complications. Conclusion. This method allows reducing intraoperative zonular stress and removing all nucleus fragments in the conditions of limited mydriasis at the maximum distance from the corneal endothelium. The rotationless technology of nucleous removal is effective and safe at cataract in PEX.

Pupillary dilation as a thermal stress indicator in boer crossbred goats maintained in a climate chamber

Goats are homeothermic animals considered rustic from the bioclimatic point of view, but a reduction in their productive efficiency occurs under unfavorable thermal conditions. The present study evaluated pupil dilation as an indicator of thermal stress in Boer crossbred goats maintained under controlled environmental conditions. Six male crossbred goats of the Boer breed were distributed in a completely randomized design and submitted to temperatures of 26, 29 and 33°C and 68% relative humidity. Pupillary dilation (PD), pupillary temperature (PT), respiratory frequency (FR), heart rate (HR), surface and rectal temperatures (ST and RT), body weight gain (BWG) and feed conversion (FC) were monitored. An increase in the PD of the animals was observed with elevated air temperature. Specifically, pupillary area increased by 26.96mm2 at 33°C compared to 26°C. PD is shown as an efficient response for measuring the level of thermal stress in goats.

Pupillary light reflex, receptive field mechanism and correction for retinal position for the assessment of visual discomfort

Light sources causing annoyance or pain produce discomfort glare. Traditional glare metrics fail for non-uniform luminaires. As an alternative, visual discomfort is determined by a model incorporating the centre–surround receptive field mechanism, the pupillary light reflex and a correction for retinal position. The pupil area, controlled by the pupillary light reflex, regulates the retinal illuminance. A centre–surround receptive field, described by a difference of Gaussians, represents the visual signal. A correction according to the Guth position index accounts for the reduction in brightness perception when a light source is moved away from the line of sight. The model is analysed with a forced choice paired comparison experiment involving 17 non-uniform rear projected stimuli with different spatial frequencies and luminance steps. A coefficient of determination of 0.68 between the subjective assessment and the model is obtained. A paired comparison office luminaire experiment and a magnitude estimation experiment involving diffusor luminaires validate the model resulting in a coefficient of determination of 0.86 and 0.81, respectively. By including the pupillary light reflex, receptive field mechanism and a correction for retinal position, the more physiologically justified model is a promising alternative to current, often empirical, glare metrics, especially for non-uniform luminaires.

Factors Affecting Mydriasis-Free Flicker ERGs Recorded With Real-Time Correction for Retinal Illuminance: Study of 150 Young Healthy Subjects.

A small, full-field flicker electroretinogram (ERG) recording system was recently developed to record flicker ERGs without mydriasis (RETeval). The device delivers a stimulus with constant retinal illuminance by adjusting the retinal luminance to compensate for changes in the pupillary area. The purpose of this study was to determine what factors affect the fundamental components of the flicker ERGs recorded by RETeval in young healthy subjects. Flicker ERGs were recorded with the RETeval system from 150 eyes of 150 young healthy subjects (age, 20-29 years). Univariate and multivariate linear regression analyses were performed to identify the factors that affected the implicit times and amplitudes of the fundamental component of the flicker ERGs. The independent variables included age, sex, refractive error, axial length, and pupillary area. Multivariate regression analyses indicated that a longer axial length (P = 0.03) and larger pupillary area (P = 0.008) were independent factors that were significantly associated with longer implicit times of the fundamental component of the flicker ERGs. Multivariate regression analyses also showed that the female sex (P = 0.03) was an independent factor, which was significantly associated with larger amplitude fundamental component of the flicker ERGs. These results indicate that the fundamental components of the RETeval flicker ERGs are significantly affected by the axial length, pupillary area, and sex of young healthy subjects. The results also suggest that it would be better to compensate for the Stiles-Crawford effect when flicker ERGs are recorded with natural pupils.