Pupicidal Activity Research Articles

Characterization and application of rhamnolipid from Pseudomonas plecoglossicida BP03.

The production of rhamnolipid (glycolipid) biosurfactant was achieved under optimized conditions from newly isolated bacteria (Pseudomonas plecoglossicida BP03) from rice mill effluent. The isolated biosurfactant was structurally characterized using FTIR and NMR spectroscopic studies. The obtained biosurfactant (1·39gl-1 ) showed a variety of applications including larvicidal and pupicidal activity against malarial vector (Anopheles sunadicus). It also exhibited antimicrobial activity against human pathogens, and possessed potent anti-biofilm activity against Staphylococcus aureus, Bacillus subtilis and Aeromonas hydrophila. The obtained biosurfactant showed a dose-dependent inhibition of exopolymeric substance (EPS) and growth curve in S. aureus. Furthermore, the cytotoxicity assays revealed that the biosurfactant exhibit a cytotoxic potency against the human fibroblastic sarcoma cells Ht-1080. An in silco analysis was also performed using Schrodinger maestro 9.3 against surface protein (SasG) of S. aureus, and the resultant analysis revealed an interactive docking score of -3·4kcalmol-1 . The obtained result indicates that the synthesized economically viable biosurfactant ensures excellent applications towards various fields.

Nanoemulsion Loaded with Volatile Oil from Piper alatipetiolatum as an Alternative Agent in the Control of Aedes aegypti

Diseases transmitted by the mosquito Aedes aegypti, Culicidae, are a serious public health problem worldwide, especially because of the increasing resistance to synthetic insecticides. Volatile oils are botanicals with interesting multi-target properties, but with reduced applications due to its volatility and water solubility. For this reason, nanoemulsions containing volatile oils are an important alternative for the mosquito control. The volatile oil of leaves Piper alatipetiolatum Yunck., Piperaceae, was characterized mostly by sesquiterpenes, such as ishwarone (78.6%), ishwarol B (8.2%), β-elemene (6.9%), selin-11-en-4α-ol (2.9%), and ishwarane (2.4%). The volatile oil showed lower ovicidal activity (19 to 100%), larvicidal activity (LC50 33.74 ppm), and pupicidal activity (LC50 65.06 ppm) as compared with its nanoemulsion that presented higher ovicidal activity (47.7 to 100%), larvicidal activity (LC50 6.37 ppm), and pupicidal activity (LC50 9.33 ppm) against Ae. aegypti. The volatile oil nanoemulsion was characterized with approximately spherical morphology, with an average size of 316 ± 8 nm, PDI of 0.44 ± 0.01, zeta potential of − 8.5 ± 0.1 mV, and pH of 5.3 ± 0.1. These results indicated that the nanoemulsion containing the encapsulated volatile oil was effective to interrupt the development of immature forms of Ae. aegypti and represents an efficient alternative tool to control this vector, contributing to vector control of dengue and other related diseases.

Deployment and Fact Analysis of the In2Care® Mosquito Trap, A Novel Tool for Controlling Invasive Aedes Species.

During April-October 2019, the West Valley Mosquito and Vector Control District (Ontario, CA) deployed large numbers of In2Care® mosquito traps in a preliminary study to evaluate the trap's potential effectiveness at controlling invasive Aedes aegypti (L.) and Ae. albopictus (Skuse) in 6 cities of San Bernardino County, CA. The trap was used to attract ovipositing females, expose them to the juvenile hormone mimic pyriproxyfen and the entomopathogenic fungus Beauveria bassiana, and autodisseminate pyriproxyfen to other water sources prior to their death from fungal infection. The trap attracted Ae. aegypti and Culex quinquefasciatus, with the latter species predominating at much higher larval densities in the trap reservoirs. Field-collected larvae and pupae from the trap reservoirs showed complete adult emergence inhibition. Furthermore, the trap reservoirs retained high levels of residual larvicidal, pupicidal, and emergence inhibition activity after they were retrieved from the field, as indicated by laboratory bioassays against laboratory colony of Cx. quinquefasciatus. Results of this study support more detailed quantitative local evaluations on trap efficacy to measure the impact of the In2Care mosquito trap on wild invasive Aedes and Culex populations in future mosquito control efforts.

Residual Larvicidal Activity of Quinones against Aedes aegypti.

The number of documented dengue cases has increased dramatically in recent years due to transmission through the Aedes aegypti mosquito bite. Vector control remains the most effective measure to protect against this and other arboviral diseases including Zika, chikungunya and (urban) yellow fever, with an established vaccine only available for yellow fever. Although the quinone class shows potential as leading compounds for larvicide development, limited information restricts the development of optimized structures and/or formulations. Thus, in this contribution we investigated the larvicidal and pupicidal activity of three quinone compounds isolated from a Connarus suberosus root wood ethyl acetate extract together with 28 quinones from other sources. Eight quinones demonstrated larvicidal activity, of which tectoquinone (4) proved to be the most active (LC50 1.1 µg/mL). The essential residual effect parameter of four of these quinones was evaluated in laboratory trials, with tectoquinone (4) and 2-ethylanthraquinone (7) presenting the most prolonged activity. In small-scale field residual tests, tectoquinone (4) caused 100% larvae mortality over 5 days, supporting its selection for formulation trials to develop a prototype larvicide to control Ae. aegypti.

Green synthesis of silver nanoparticle using Leonotis nepetifolia and their toxicity against vector mosquitoes of Aedes aegypti and Culex quinquefasciatus and agricultural pests of Spodoptera litura and Helicoverpa armigera.

Pest insects causing damage to cultivable crops and food products by feeding, fecundity, and parasitizing livestock, also being a nuisance to human health. In consideration with human health, the World Health Organization reports that more than 50% of the world's population is presently at risk from mosquito-borne diseases. Mosquitoes are primary vectors for major dreadful diseases such as yellow fever, malaria, and dengue fever, which infect millions of human beings all over the world and kill millions of peoples every year. The present research work was carried out to evaluate the antifeedant, larvicidal, pupicidal, larval, and pupal duration activity of Leonotis nepetifolia-mediated silver nanoparticles (AgNPs) against Spodoptera litura, Helicoverpa armigera, Aedes aegypti, and Culex quinquefasciatus. Biosynthesized AgNPs were characterized through various techniques such as UV-Vis spectrometer, X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential analysis. The AgNPs showed potential antifeedant activity of 78.77% and 82.16% against the larvae of S. litura and H. armigera, respectively. The maximum larval mortality rate (78.49% and 72.70%) and maximum pupal mortality rate (84.66% and 77.44%) were observed against S. litura and H. armigera. Mosquito larvae were tested with biosynthesized AgNPs, and recorded LC50 values were 47.44ppm and 35.48ppm on A. aegypti and C. quinquefasciatus, respectively. The histological examinations showed that the acceleration of the nanomaterial caused severe tissue damage in the epithelial and goblet cells in the larval midgut region of S. litura, H. armigera, A. aegypti, and C. quinquefasciatus. Biosynthesis of silver nanoparticles using L. nepetifolia is an ideal eco-friendly approach for the management of insect pests. Graphical abstract.

Evaluation of the efficacy of benzothiazole against the red flour beetle, Tribolium castaneum (Herbst).

In the context of the resistance development and health risks of currently used fumigants, it is urgent to seek more effective and ecofriendly compounds for stored-product pest control. The microbial volatile compound benzothiazole is known to have fungicidal and insecticidal activity; however, its detailed efficacy on storage pests is largely unknown. Benzothiazole was identified for its great ovicidal, larvicidal, pupicidal and adulticidal activity against Tribolium castaneum, and exhibited potent repellency against T. castaneum. The benzothiazole concentrations and developmental stage of T. castaneum were the key factors affecting the insecticidal effects. Adults of T. castaneum exposed to benzothiazole for as long as 168 h showed a decrease in progeny production. Based on 7 days of fumigation in the model food system, benzothiazole at 0.12 mg mL-1 provided an efficacy of 96% and completely inhibited the number of offspring. Safety profile assessment showed that benzothiazole did not affect the germination rate of wheat seeds but had a slight negative effect on seedling growth. However, sufficient ventilation and soil nutrients could relieve this adverse impact. Benzothiazole is a strong fumigant and repellent against T. castaneum. This study provides a good perspective of novel ways to control T. castaneum. © 2020 Society of Chemical Industry.

Biocontrol efficacy of bay essential oil against housefly, Musca domestica (Diptera: Muscidae)

BackgroundThe synanthropic housefly, Musca domestica, augments the transmission of several detrimental diseases like cholera and avian flu. Consequently, during the last century, many physico-chemical methods including synthetic compounds have been applied for its control. But these methods have proven to be prohibitive due to their side effects and serious issues like resistance development, environmental contamination, and detrimental effects on non-target fauna. Therefore, in view of these objectives, we investigated the effects of bay essential oil (EO) against M. domestica.MethodsThe attractant/repellent assays were conducted by double choice technique. Different enzyme assays evaluating the effect of LC50 concentration of the tested essential oil on larval gut were taken into consideration. To determine the composition, the tested oil was subjected to GC-MS/MS analysis. Further, the morphological alterations caused by EO treatment to third instar larvae were observed in a Nova Nano SEM machine. Data was statistically analyzed by one-way ANOVA using Tukey’s test (p < 0.001). The LC50 and LC90 values were calculated by probit analysis.ResultsThe adulticidal bioassay revealed significant effects with LC50 concentration as 43.03 mg/dm3 against the newly emerged adult flies while in larvicidal assay mortality was dose dependent showing maximum effect at LC50 0.0629 μg/cm2. The pupicidal activity was more effective at a dose of LD50 64.09 μl/0.25 L of air which either killed the pupae or caused deformity in the emerged adults. Likewise total sugar, protein, glycogen, and lipid contents of larvae were reduced after treatment with EO when compared with the normal larvae along with some gut enzymes. The EO reduced the acetylcholinesterase activity from 0.013 U/mg protein in normal larvae to 0.0093 U/mg protein after EO treatment. The GC-MS/MS analysis of the bay EO showed the abundance of myrcene, linalool, eugenol, chavicol, and anethole along with diterpenoid, geranylgeraniol. However, the insecticidal activity of tested EO might be majorly imparted by eugenol content. The FESEM analysis showed shrinkage of integument and distortion to intersegmental regions caused by the tested compound.ConclusionThe present study concludes the significant efficacy of bay EO against M. domestica which could be employed to breakdown its population below threshold levels to prevent the menace of vector-borne diseases.

Target and Nontarget Toxicity of Cassia fistula Fruit Extract Against Culex pipiens (Diptera: Culicidae), Lung Cells (BEAS-2B) and Zebrafish (Danio rerio) Embryos.

Mosquitoes transmit serious diseases, which threaten humans and severely affect livestock. The half-lethal concentration (LC50) was calculated by log probit analysis. The LC50 and LC90 values of larvicidal activity of Cassia fistula Linn. hexane-methanol soluble fraction (HMSF) after 24 h of exposure were 21.04 and 34.68 µg/ml, respectively. The LC50 values after 24 h of exposure were 84.09 µg/ml and 108.08 µg/ml for chloroform-methanol soluble fraction (CMSF) and ethyl acetate-methanol soluble fraction (EMSF) respectively. The percent hatchability of eggs exposed to the hexane extract was 90 ± 5.0, 68.33 ± 7.6, 46.6 ± 11.5, 10 ± 0.0, and 0 ± 0.0% at 10, 20, 40, 60, and 80 ppm, respectively. The pupicidal activity of the hexane extract at 40 µg/ml was 0.0%. The LC50 value of adulticidal activity of the hexane extract was 12.8 mg/test tube. The biosafety of the hexane extract was assessed in nontarget organisms, i.e., zebrafish (Danio rerio) embryos and normal lung cells (BEAS-2B). The hexane extract of C. fistula was well tolerated by zebrafish embryos, and no mortality or toxicity was found in the embryos exposed to the highest tested concentration of 300 µg/ml. Similarly, all the concentrations tested against the normal lung cells (BEAS-2B) showed more than 95% survival. The gas chromatography-mass spectroscopy analysis identified 12 compounds, and 2-methyl hexanoic acid and 2-methyl butanoic acid were the major compounds identified in the hexane extract. The larvicidal activity of C. fistula extracts will help in the development of natural substitutes for vector management of mosquito populations.

Insecticidal Activities of Chromolaena odorata and Vernonia amygdalina leaf extracts against Anopheles gambiae [Diptera: Culicidae

Female Anopheles mosquitoes are the vectors of human malaria. The use of chemical insecticides for vector control has hampered with environmental pollution and insect. This suggests the need for the development of more potent and environment-friendly insecticides for effective control of malaria.This research investigated the larvicidal, pupicidal and adulticidal activities of Chromolaena odorata and Vernonia amygdalina leaf extracts against, An. gambiae in the laboratory at ambient temperature of 28 ± 2 °C and 75 ± 5% relative humidity. Different concentrations of 20 mg/L, 40 mg/L, 80 mg/L, 120 mg/L and 160 mg/L were prepared and these aqueous solutions were used for the experiments. Larval, pupal and adult mortality of An. gambiae were tested after 24 hours of exposure. Results showed that there were significant differences (P < 0.05) in toxicity level of the two plant extracts on An. gambiae larvae, pupae and adults. Vernonia amygdalina extract was the most toxic to An. gambiae larvae at all tested concentrations of 20 mg/L, 40 mg/L, 80 mg/L, 120 mg/L and 160 mg/L causing 47.5%, 82.5%, 100%, 100% and 100% mortality after 24 hours of treatment, respectively.

ANTIOXIDANT ACTIVITY, MOSQUITOCIDAL ACTIVITY, ANTIBACTERIAL ACTIVITY OF SOLVENT EXTRACT AND SYNTHESIZED NANOPARTICLES OF CLAUSENA EXCAVATA (BURM.) F. (RUTACEAE) AGAINST SELECTED MOSQUITOES AND PATHOGENIC BACTERIA

In the present investigation, Clausena excavata an indegeous plant was sequentially extracted with hexane, ethyl acetate and methanol and their bio efficacy in terms of Antioxidant activity, mosquitocidal activity and antibacterial activity was tested. Among the three-extract methanol extract of C. excavata displayed significant antioxidant, larvicidal, ovicidal, pupicidal activities against Aedes aegypti and Culex quinquefasciatus. Similarly, methanol extract also showed pronounced antibacterial activity against the selected human pathogenic bacteria. Thus, the methanol extract of C. excavata was used to synthesize silver mediated nanoparticles and its (C. excavata AgNPs) bio efficacy was also tested with the selected vector mosquitoes and selected human pathogenic bacteria. This present investigation envisages the possible utilization of C. excavata as a potent candidate as green pesticides in the near future. Article DOI: https://dx.doi.org/10.20319/lijhls.2019.51.1939 This work is licensed under the Creative Commons Attribution-Non-commercial 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.

Retraction Note to: Mosquitocidal properties of Bacillus species isolated from mangroves of Vellar estuary, Southeast coast of India.

[This retracts the article DOI: 10.1007/s12639-013-0371-9.].

Larvicidal and pupicidal activities of eco-friendly phenolic lipid products from Anacardium occidentale nutshell against arbovirus vectors.

Aedes aegypti and Culex quinquefasciatus are vectors of diseases that constitute public health problems. The discovery of products capable of inhibiting their development which are less harmful to the environment would have a huge impact on vector control. Here, natural cashew nut shell liquid (CNSL), technical CNSL, anacardic acid, cardanol, and cardol were isolated from Anacardium occidentale and evaluated for larvicidal and pupicidal activity against Ae. aegypti and Cx. quinquefasciatus under laboratory and field conditions. The activities of phenol, resorcinol, salicylic acid, and pentadecane, commercial chemicals similar in structure to nut shell derivatives, were also evaluated. All of the fractions extracted from A. occidentale oil exerted larvicidal effects against both mosquito species (LC50 5.4-22.6mg/L), and two of the aforementioned were effective against pupae (LC50 90.8-109.7mg/L). Of all the fractions tested, cardol demonstrated the strongest larvicidal and pupicidal effects and presented the most prolonged residual activity against the larvae and pupae of Ae. aegypti and Cx. quinquefasciatus under field conditions. This study suggests that A. occidentale nut shell derivatives are sustainable and promising candidates for the development of novel insecticides to overcome the problem of harmful chemical insecticides.

GC–MS analysis and anti–mosquito activities of Juniperus virginiana essential oil against Anopheles stephensi (Diptera: Culicidae)

Objective: To investigate phytochemicals present in the essential oil from aerial parts of eastern red cedar, Juniperus virginiana (J. virginiana) L. (Cupressaceae) and to determine its killing and repellent activities against larvae, pupae, and adults of the Asian malaria mosquito, Anopheles stephensi (Diptera: Culicidae). Methods: J. virginiana essential oil was extracted by hydrodistillation, and its chemical composition was determined by gas chromatography-mass spectrometry. Seven different logarithmic concentrations of J. virginiana essential oils were used in larvicidal and pupicidal assays. J. virginiana essential oils-impregnated bed nets were applied in a designed animal module to test excito-repellent activity against adult mosquitoes. Results: Fourteen constituents corresponding to 99.98% of J. virginiana essential oils were identified. Five main components were terpinen-4-ol (25.21%), camphor (19.89%), E-3-hexen- 1-ol (13.30%), γ-terpinene (7.86%), and l-menthone (2.27%). The LC50 and LC90 values against larvae of the Anopheles stephensi were 11.693 and 66.140 ppm and for pupae were 9.640 and 40.976 ppm, respectively. In excito-repellency assay, J. virginiana essential oils-impregnated bed nets provided an average of 54.63% protection for guinea pig and 45.37% mortality for the mosquitoes. Conclusions: Four monoterpenes and one leaf alcohol were identified by gas chromatography-mass spectrometry. J. virginiana essential oils showed potent larvicidal, pupicidal, adulticidal, and repellent activities against Anopheles stephensi at acceptable concentrations. Evaluation of bioactivity of identified chemicals (alone or in combination) will provide new eco-friendly substances for mosquito-management programs.

Lagenaria siceraria - synthesised ZnO NPs - a valuable green route to control the malaria vector Anopheles stephensi.

Malaria is a dangerous disease affecting humans and animals in tropical and subtropical areas worldwide. According to recent estimates, 3.2 billion people are at risk of malaria. Many drugs are in practices to control this disease and their vectors. Eco-friendly control tools are needed to fight vectors of this important disease. Nanotechnology is playing a key role in the fight against many public health emergencies. In the present study, Lagenaria siceraria aqueous peel extract was used to prepare zinc oxide nanoparticles (ZnO NPs), then tested on Anopheles stephensi eggs, larvae and pupae. The L. siceraria-synthesised ZnO NPs were characterized additionally by FTIR, AFM, XRD, UV-Vis spectroscopy, EDX, and SEM spectroscopy The ovicidal, larvicidal, pupicidal and repellent activities of L. siceraria and green-synthesised ZnO NPs were analysed on A. stephensi. The potential mechanism of action of ZnO NPs was studied investigating the changes in various enzyme activities in A. stephensi IV instar larvae. Furthermore, the smoke toxicity of L. siceraria-based cones against A. stephensi evoked higher mortality if compared with the control. Overall, the present study concluded that L. siceraria peel extract and its mediated green synthesised ZnO NPs represent a valuable green option to manage against malaria vectors.

Larvicidal and Pupicidal activity of Clerodendrum philippinum Schauer Leaf Extracts against Anopheles stephensi and Aedes aegypti

Larvicidal and Pupicidal activity of Clerodendrum philippinum Schauer Leaf Extracts against Anopheles stephensi and Aedes aegypti

Toxicity And Physiological Activity of Methoprene, A Juvenile Hormone Analog, Against Development and Metamorphosis of The Grey Flesh Fly, Parasarcophaga argyrostoma (Robineau- Desvoidy)(Diptera: Sarcophagidae).

The grey flesh fly Parasarcophaga argyrostoma plays a role in human cutaneous wounds and eye myiasis and it is also known as parasitoid of various animals. The present study aimed to investigate the efficacy of Methoprene on survival, development and metamorphosis of this fly species. Five dose levels (10.0, 5.0, 1.0, 0.1 and 0.01µg/larva) of Methoprene was topically applied onto the early last instar larvae and prepupae. Methoprene exhibited larvicidal, pupicidal and adulticidal activities against P. argyrostoma. LD50 values were found 0.155 and 0.258 µg/insect after topical treatment of early last instar larvae and prepupae, respectively. The maximal body weight of treated larvae was considerably decreased. The duration of treated larvae was prolonged. The coefficient of growth of treated larvae was depressed. The pupal duration was remarkably prolonged. Some larval-pupal intermediates had been produced, only at the higher two doses. Topical treatment of prepupae only with the lower two doses induced a state of 'permanent prepupae'. The treated last instar larvae pupated in regressed rate. The pupation rate was slightly regressed after topical treatment of prepupae only with the lower two doses. The adult emergence of flies was completely blocked after topical treatment of either the early last instar larvae or prepupae with the highest dose of Methoprene. At other dose levels, the adult eclosion of flies was partially blocked. Different percentages of deformed pupaeand adults were recorded.

Screening of Larvicidal and Pupicidal Activity Of Biogenic Silver Nanoparticles (AgNPs) of Bidens biternata(L) against the Dengue &amp; Chikungunya Vector, Aedes Aegypti

Screening of Larvicidal and Pupicidal Activity Of Biogenic Silver Nanoparticles (AgNPs) of Bidens biternata(L) against the Dengue &amp; Chikungunya Vector, Aedes Aegypti

Molecular characterization of bmyC gene of the mosquito pupicidal bacteria, Bacillus amyloliquefaciens (VCRC B483) and in silico analysis of bacillomycin D synthetase C protein.

A strain of Bacillus amyloliquefaciens (VCRC B483) exhibiting mosquito pupicidal, keratinase and antimicrobial activities was isolated from mangrove forest ecosystem of Andaman and Nicobar Islands. Molecular characterization of the strain showed the presence of lipopeptide encoding bmyC gene. Phylogenetic tree based on protein sequence of this gene exhibited homology with mycosubtilin synthetase of Bacilus atropheus and Iturin synthetase of Bacillus subtilis and B. amyloliquefaciens. This is the first report on the evolutionary conservation of amino acids concerned with the function and structure of bmyC protein of B. amyloliquefaciens. The presence of valine at the 1197th position in our strain was found to be unique and different from the existing strains of B. subtilis and B. amyloliquefaciens. Molecular modelling studies revealed significant changes in the structure of epimerization domain of the bmyC protein with A1197V variation. Crude metabolite of this strain exhibited antifungal activity against Fusarium sp. and Carvularia sp.

Bio-pesticidal effects of Trichoderma viride formulated titanium dioxide nanoparticle and their physiological and biochemical changes on Helicoverpa armigera (Hub.)

The control of agricultural pests through eco-friendly nanopesticides is a challenge of crucial environmental importance nowadays. The current study was aimed to discover a novel biopesticides through Trichoderma viride mediated synthesis of titanium dioxide nanoparticles (TDNPs). The main chemical and physical features of the TDNPs were assessed by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), energy dispersive X-ray (EDX) and size distribution and shape of the NPs studied through the scanning electron microscope (SEM), high-resolution transmission electron microscope (HR-TEM) and dynamic light scattering (DLS). The extracellular synthesized nanoparticles were evaluated for their larvicidal, antifeedant and pupicidal activities against Helicoverpa armigera. TDNPs exhibited highest mortality rate on first (100%), second (100%) and third (92.34%), instar larvae of H. armigera at 100 ppm. The detoxifying enzymes such as, β-glucosidase and carboxylesterase were reduced whereas glutathione S-transferase increased during the treatment of TDNPs against H. armigera at 100 ppm. No toxic effects were found on Eudrilus eugeniae filter paper and artificial soil assays treated with TDNPs at 100 ppm. However, cypermethrin was toxic to earthworms after 72 h treatment. Therefore, TDNPs could act as significant inhibitors on the development of H. armigera, although, no adverse effect was found on earthworms.

Larvicidal, pupicidal and oviposition deterrent activities of essential oils from Umbelliferae plants against house fly Musca domestica

Objective: To evaluate the toxicity against house fly Musca domestica L. (M. domestica)-larvicidal, pupicidal, and oviposition deterrent activities of essential oils (EOs) from five plants: Anethum graveolens L. (A. graveolens), Centratherum anthelminticum L. (C. anthelminticum), Foeniculum vulgare Mill. (F. vulgare), Pimpinella anisum L. (P. anisum), and Trachyspermum ammi L. (T. ammi) Sprague. Methods: Dipping, topical and dual-choice assays were performed by using each EO at three concentrations (1%, 5% and 10%) as well as cypermethrin (positive control) and ethyl alcohol (negative control) in order to determine their larvicidal, pupicidal and oviposition deterrent activities against M. domestica mortality rate and morphogenic abnormality of larvae and pupae were recorded after 3 and 10 days of incubation. Oviposition deterrent activity was recorded for 5 consecutive days from the beginning of the assay. Results: All EOs at the highest concentration (10%) were highly effective in oviposition deterrent with 100% effective repellency and an oviposition activity index of -1.00. Ten percent of F. vulgare oil exhibited the highest efficacy against the larvae with 89.6% mortality rate at 3 days and an LC50 value of 5.4%. Ten percent of A. graveolens and T. ammi oils showed the highest efficacy against the pupae with 100% mortality and an LC50 value ranging from 1.81%-2.24%. Conclusions: These results indicate that F. vulgare, A. graveolens and T. ammi EOs are effective larvicidal, pupicidal and oviposition deterrent agents against house fly M. domestica.