This study examines the effects of an additional transmission chamber on a pulsed jet in crossflow using experimental and Large-eddy simulation methods, with a focus on the structural and mixing characteristics of the flow. Three pulse frequencies are selected (f = 20 Hz, 50 Hz, and 100 Hz), and the results of the model with an additional transmission chamber are compared with the round pipe model. The results show that the additional transmission chamber enhances jet penetration, diffusion, and mixing uniformity, particularly at higher pulse frequencies. The additional transmission chamber also alters the exit velocity profile, increasing peak velocity and promoting greater deflection under crossflow. These findings highlight the potential of transmission chambers to optimize pulsed jet performance in applications requiring effective mixing and penetration.