We study how a Rabi oscillation is affected by a periodic pulsed perturbation. Unlike a continuous perturbation, which simply shifts the resonance frequency, a pulsed perturbation splits the Rabi resonance into two lines separated by the pulse repetition rate. When the perturbation integrated over one pulse is equal to $\ensuremath{\pi}$, the resonance splits into two symmetrically placed lines at \ifmmode\pm\else\textpm\fi{} half the pulse repetition rate. When it is $2\ensuremath{\pi}$, the Rabi oscillation is unaffected by the perturbation. We demonstrate these behaviors using the ground hyperfine transition of cesium atoms in a magneto-optical trap. The pulsed perturbation is introduced by a periodic magnetic field pulse. The $2\ensuremath{\pi}$ case is compared with the self-induced transparency, and its implications for optical frequency metrology using a pulsed optical trap is discussed.
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