Pulse Inversion Research Articles

Hemodynamic study of unenhanced magnetic resonance angiography using spatial labeling with multiple inversion pulses sequence: a phantom study.

This study sought to explore the functional relationship between displayed vascular length and blood suppression inversion time (BSP TI) and flow velocity in a phantom, and to provide a theoretical basis for quantitatively assessing vascular hemodynamic responses using unenhanced magnetic resonance angiography (MRA) and spatial labeling with multiple inversion pulses sequence (SLEEK). A polyethylene catheter was laid in a long rectangular container filled with pork fat. The entrance of the catheter into the container was connected to a high-pressure syringe filled with normal saline. The high-pressure injector flow rates were set at 0.0, 0.2, 0.4, 0.8, 1.2, 1.6, 2.0, and 2.4 mL/s. SLEEK was performed 19 times for each flow rate with parameter BSP TI values of 50, 75, 100, 150, 200, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,100, 1,200, 1,300, 1,400, 1,600, and 1,800 ms. Maximum intensity projection was employed to reconstruct all SLEEK original images to determine the measurements of the displayed vascular lengths. A regression analysis was undertaken to assess the relationship between the displayed vascular lengths and BSP TI values for each flow rate, and to assess the relationship between the displayed vascular lengths and flow rates at each BSP TI. The displayed vascular length had a linear relationship with BSP TI for each flow rate (P<0.05) (R2=0.754, 0.941, 0.988, 0.988, 0.977, 0.966, and 0.982 for flow rates of 0.0, 0.2, 0.4, 0.8, 1.2, 1.6, and 2.0 mL/s, respectively). The displayed vascular length also had a linear relationship with flow rate for each BSP TI value (P<0.05) (R2 =0.914, 0.912, 0.834, 0.989, 0.980, 0.996, 0.992, 0.960, 0.975, 0.979, 0.982, 0.981, 0.976, and 0.993 for BSP TI 50, 75, 100, 150, 200, 300, 400, 500, 600, 700, 800, 900, 1,000, and 1,100 ms, respectively). No significant linear relationship was found between displayed vascular length and flow rate when the BSP TI value was 1,200 ms (P>0.05). Vascular displayed length has a linear relationship to BSP TI for flow ranges from 0.0 to 2.0 mL/s. Vascular displayed length has a linear relationship to flow rate for BSP TI values of 50 to 1,100 ms. Flow rate can be assessed in relation to vascular displayed length.

Numerical Engineering of Robust Adiabatic Operations

Adiabatic operations are powerful tools for robust quantum control in numerous fields of physics, chemistry and quantum information science. The inherent robustness due to adiabaticity can, however, be impaired in applications requiring short evolution times. We present a single versatile gradient-based optimization protocol that combines adiabatic control with effective Hamiltonian engineering in order to design adiabatic operations tailored to the specific imperfections and resources of an experimental setup. The practicality of the protocol is demonstrated by engineering a fast, 2.3 Rabi cycle-long adiabatic inversion pulse for magnetic resonance with built-in robustness to Rabi field inhomogeneities and resonance offsets. The performance and robustness of the pulse is validated in a nanoscale force-detected magnetic resonance experiment on a solid-state sample, indicating an ensemble-averaged inversion accuracy of $\sim 99.997\%$. We further showcase the utility of our protocol by providing examples of adiabatic pulses robust to spin-spin interactions, parameter-selective operations and operations connecting arbitrary states, each motivated by experiments.

On the Development of a Novel Contrast Pulse Sequence for Polymer-Shelled Microbubbles.

Contrast agents are routinely used in ultrasound examinations. Nonlinear ultrasound imaging techniques have been developed over decades to enhance the contrast between the tissue and the blood pool after the injection of ultrasound contrast agents (UCAs). In this study, we introduce a new contrast pulse sequence, CPS4. The CPS4 combines pulse inversion (PI), subharmonic (SH), and ultraharmonic (UH) techniques to remove propagation distortion while capturing the unique SH and UH responses from UCAs. The novel CPS4 and conventional PI, SH, and UH techniques were used to detect the presence of a research-grade, thick-shell, polymer microbubble in a tissue-mimicking flow phantom. The contrast-to-tissue ratios (CTRs) obtained from the applications of all techniques were compared. The results show that the highest CTR of approximately 16 dB was obtained using CPS4, which was superior to the individual reference techniques: PI, SH, and UH techniques, in all scenarios considered in this study.

Linear Signal Cancellation of Nonlinear Pulsing Schemes in a Verasonics Research Scanner.

Contrast-enhanced ultrasound (CEUS) is a real-time imaging technique that allows the visualization of organ and tumor microcirculation by utilizing the nonlinear response of microbubbles. Nonlinear pulsing schemes are used exclusively in CEUS imaging modes in modern scanners. One important aspect of nonlinear pulsing schemes is the near-complete elimination of the linear signals that originate from tissue. Up until now, no study has investigated the performance of Verasonics scanners in eliminating the linear signals during CEUS and, by extension, the optimal pulsing sequences for performing CEUS. The aim of this article was to investigate linear signal cancellation of the Verasonics scanner performing nonlinear pulsing schemes with two different probes (L7-4 linear array and C5-2 convex array). We have considered two pulsing schemes: pulse inversion (PI) and amplitude modulation (AM). We have also compared our results from the Verasonics scanner with a clinical scanner (Philips iU22). We found that the linear signal cancellation of the transmitted pulse by Verasonics scanner was ~40 dB in AM mode and ~30 dB in PI mode when operated at 0.06 MI. The linear signal cancellation performance of Verasonics scanner was comparable with Philips iU22 scanner in focused AM mode and on average 3 dB better than Philips iU22 scanner in focused PI mode.

Frequency-Dependent Spatial Coherence in Conventional and Chirp Transmissions.

The development of adaptive imaging techniques is contingent on the accurate and repeatable characterization of ultrasonic image quality. Adaptive transmit frequency selection, filtering, and frequency compounding all offer the ability to improve target conspicuity by balancing the effects of imaging resolution, the signal-to-clutter ratio, and speckle texture, but these strategies rely on the ability to capture image quality at each desired frequency. We investigate the use of broadband linear frequency-modulated transmissions, also known as chirps, to expedite the interrogation of frequency-dependent tissue spatial coherence for real-time implementations of frequency-based adaptive imaging strategies. Chirp-collected measurements of coherence are compared to those acquired by individually transmitted conventional pulses over a range of fundamental and harmonic frequencies, in order to evaluate the ability of chirps to recreate conventionally acquired coherence. Simulation and measurements in a uniform phantom free of acoustic clutter indicate that chirps replicate not only the mean coherence in a region-of-interest but also the distribution of coherence values over frequency. Results from acquisitions in porcine abdominal and human liver models show that prediction accuracy improves with chirp length. Chirps are also able to predict frequency-dependent decreases in coherence in both porcine abdominal and human liver models for fundamental and pulse inversion harmonic imaging. This work indicates that the use of chirps is a viable strategy to improve the efficiency of variable frequency coherence mapping, thus presenting an avenue for real-time implementations for frequency-based adaptive strategies.

Proton-detected solid-state NMR spectroscopy of spin-1/2 nuclei with large chemical shift anisotropy

Constant-time (CT) dipolar heteronuclear multiple quantum coherence (D-HMQC) has previously been demonstrated as a method for proton detection of high-resolution wideline NMR spectra of spin-1/2 nuclei with large chemical shift anisotropy (CSA). However, 1H transverse relaxation and t1-noise often reduce the sensitivity of D-HMQC experiments, preventing the theoretical gains in sensitivity provided by 1H detection from being realized. Here we demonstrate a series of improved pulse sequences for 1H detection of spin-1/2 nuclei under fast MAS, with 195Pt SSNMR experiments on cisplatin as an example. First, a t1-incrementation protocol for D-HMQC dubbed Arbitrary Indirect Dwell (AID) is demonstrated. AID allows the use of arbitrary, rotor asynchronous t1-increments, but removes the constant time period from CT D-HMQC, resulting in improved sensitivity by reducing transverse relaxation losses. Next, we show that short high-power adiabatic pulses (SHAPs), which efficiently invert broad MAS sideband manifolds, can be effectively incorporated into 1H detected symmetry-based resonance echo double resonance (S-REDOR) and t1-noise eliminated (TONE) D-HMQC experiments. The S-REDOR experiments with SHAPs provide approximately double the dipolar dephasing, as compared to experiments with rectangular inversion pulses. We lastly show that sensitivity and resolution can be further enhanced with the use of swept excitation pulses as well as adiabatic magic angle turning (aMAT).

Effect of a Radiotherapeutic Megavoltage Beam on Ultrasound Contrast Agents

Collateral damage to healthy surrounding tissue during conventional radiotherapy increases when deviations from the treatment plan occur. Ultrasound contrast agents (UCAs) are a possible candidate for radiation dose monitoring. This study investigated the size distribution and acoustic response of two commercial formulations, SonoVue/Lumason and Definity/Luminity, as a function of dose on clinical megavoltage photon beam exposure (24 Gy). SonoVue samples exhibited a decrease in concentration of bubbles smaller than 7 µm, together with an increase in acoustic attenuation and a decrease in acoustic scattering. Definity samples did not exhibit a significant response to radiation, suggesting that the effect of megavoltage photons depends on the UCA formulation. For SonoVue, the influence of the megavoltage photon beam was especially apparent at the second harmonic frequency, and can be captured using pulse inversion and amplitude modulation (3.5-dB decrease for the maximum dose), which could eventually be used for dosimetry in a well-controlled environment.

Phase-Aberration Correction for HIFU Therapy Using a Multielement Array and Backscattering of Nonlinear Pulses.

Phase aberrations induced by heterogeneities in body wall tissues introduce a shift and broadening of the high-intensity focused ultrasound (HIFU) focus, associated with decreased focal intensity. This effect is particularly detrimental for HIFU therapies that rely on shock front formation at the focus, such as boiling histotripsy (BH). In this article, an aberration correction method based on the backscattering of nonlinear ultrasound pulses from the focus is proposed and evaluated in tissue-mimicking phantoms. A custom BH system comprising a 1.5-MHz 256-element array connected to a Verasonics V1 engine was used as a pulse/echo probe. Pulse inversion imaging was implemented to visualize the second harmonic of the backscattered signal from the focus inside a phantom when propagating through an aberrating layer. Phase correction for each array element was derived from an aberration-correction method for ultrasound imaging that combines both the beamsum and the nearest neighbor correlation method and adapted it to the unique configuration of the array. The results were confirmed by replacing the target tissue with a fiber-optic hydrophone. Comparing the shock amplitude before and after phase-aberration correction showed that the majority of losses due to tissue heterogeneity were compensated, enabling fully developed shocks to be generated while focusing through aberrating layers. The feasibility of using a HIFU phased-array transducer as a pulse-echo probe in harmonic imaging mode to correct for phase aberrations was demonstrated.

Rapid high-resolution volumetric T1 mapping using a highly accelerated stack-of-stars Look Locker technique

PurposeTo develop a fast volumetric T1 mapping technique. Materials and methodsA stack-of-stars (SOS) Look Locker technique based on the acquisition of undersampled radial data (>30× relative to Nyquist) and an efficient multi-slab excitation scheme is presented. A principal-component based reconstruction is used to reconstruct T1 maps. Computer simulations were performed to determine the best choice of partitions per slab and degree of undersampling. The technique was validated in phantoms against reference T1 values measured with a 2D Cartesian inversion-recovery spin-echo technique. The SOS Look Locker technique was tested in brain (n = 4) and prostate (n = 5). Brain T1 mapping was carried out with and without kz acceleration and results between the two approaches were compared. Prostate T1 mapping was compared to standard techniques. A reproducibility study was conducted in brain and prostate. Statistical analyses were performed using linear regression and Bland Altman analysis. ResultsPhantom T1 values showed excellent correlations between SOS Look Locker and the inversion-recovery spin-echo reference (r2 = 0.9965; p < 0.0001) and between SOS Look Locker with slab-selective and non-slab selective inversion pulses (r2 = 0.9999; p < 0.0001). In vivo results showed that full brain T1 mapping (1 mm3) with kz acceleration is achieved in 4 min 21 s. Full prostate T1 mapping (0.9 × 0.9 × 4 mm3) is achieved in 2 min 43 s. T1 values for brain and prostate were in agreement with literature values. A reproducibility study showed coefficients of variation in the range of 0.18–0.2% (brain) and 0.15–0.18% (prostate). ConclusionA rapid volumetric T1 mapping technique was developed. The technique enables high-resolution T1 mapping with adequate anatomical coverage in a clinically acceptable time.

Real-time magnetic resonance imaging in pediatric radiology \u2014 new approach to movement and moving children

The recent development of highly undersampled radial gradient echo sequences in combination with nonlinear inverse image reconstruction now allows for MRI examinations in real time. Image acquisition times as short as 20 ms yield MRI videos with rates of up to 50 frames per second with spin density, T1- and T2-type contrast. The addition of an initial 180° inversion pulse achieves accurate T1 mapping within only 4 s. These technical advances promise specific advantages for studies of infants and young children by eliminating the need for sedation or anesthesia. Our preliminary data demonstrate new diagnostic opportunities ranging from dynamic studies of speech and swallowing processes and body movements to a rapid volumetric assessment of brain cerebrospinal fluid spaces in only few seconds. Real-time MRI of the heart and blood flow can be performed without electrocardiogram gating and under free breathing. The present findings support the idea that real-time MRI will complement existing methods by providing long-awaited diagnostic options for patients in early childhood. Major advantages are the avoidance of sedation or anesthesia and the yet unexplored potential to gain insights into arbitrary body functions.

Optimization of phase dispersion enables broadband excitation without homonuclear coupling artifacts

In NMR spectroscopy, many specialized shaped pulses are available for broadband excitation, beyond the bandwidth of conventional high-powered hard pulses. These shaped pulses typically have long duration. However, long-duration pulses are unsuitable for spectra containing significant homonuclear couplings, such as polyfluorinated compounds in 19F NMR. J-coupling evolution during the excitation pulse leads to spectral artifacts and incorrect peak integrals. Here, we report an approach to optimal control pulse design which significantly reduces the pulse length required to excite large bandwidths of chemical shift frequencies. The target state phase is not chosen beforehand but is instead only constrained to be linearly dependent on offset frequency. The first-order phase of the target state is then treated as a free-variable, to be optimized at the same time as the RF waveform itself. The resulting spectra are easily phased using standard NMR processing software. We observe that the required pulse length is significantly shorter than for currently available in-phase excitation schemes. Spectral artifacts from homonuclear couplings are avoided. We also demonstrate that pure in-phase excitation can be obtained over the same bandwidth by appending two inversion pulses, at the expense of increased overall duration.

Non-contrast renal MRA using multi-shot gradient echo EPI at 3-T MRI.

The purpose of this study was to investigate the feasibility of non-contrast renal MRA using multi-shot gradient echo planar imaging (MSG-EPI) with a 3-T MRI system. Seventeen healthy volunteers underwent non-contrast renal MRA using MSG-EPI and balanced steady-state free precession (b-SSFP) sequences on a 3-T MRI system. Two radiologists independently recorded the images' contrast, noise, sharpness, artifacts, and overall quality on 4-point scales. The signal-to-noise ratio (SNR) for the renal artery, the contrast ratio (CR) between the renal artery and erector spinae, and acquisition time were compared between the two sequences. The SNR and CR were significantly higher with MSG-EPI than with the b-SSFP sequence (17.80 ± 3.67 vs. 10.84 ± 2.86 and 0.77 ± 0.05 and 0.66 ± 0.09, respectively; p < 0.05), and the acquisition time was significantly lower (164.5 ± 34.0 vs. 261.5 ± 39.3 s, respectively; p < 0.05). There were significant differences in image contrast, noise, sharpness, artifacts, and overall image quality between the two sequences (p < 0.01). The MSG-EPI sequence is a promising technique that can shorten the scan time and improve the image quality of non-contrast renal MRA with a 3-T MRI system. • The multi-shot gradient echo planar imaging with an inversion pulse is a brand-new fast scan technique for an unenhanced renal MRA. • The image quality of multi-shot gradient echo planar imaging is better than that of b-SSFP for an unenhanced renal MRA.

Isotropic solid-state MQMAS NMR spectra for large quadrupolar interactions using satellite-transition selective inversion pulses and low rf fields

Multiple-quantum magic-angle spinning (MQMAS) pulse sequences are presented that are capable of obtaining isotropic NMR spectra for large quadrupolar interactions using lower rf fields. These experiments rely on rotor period long pulses applied at a large offset from the central-transition, making them selective to the satellite-transitions. Each such pulse gives rise to an anisotropic phase, which can be cancelled to obtain coherent signal evolution if a pair of pulses are applied in a symmetric manner. Thus, efficient excitation and conversion of triple-quantum coherences from and to the central-transition is achieved for MQMAS even for large quadrupolar couplings, by selective inversion of the satellite-transitions using such low-power pulses. Low-power multiple-quantum magic-angle spinning (lpMQMAS) pulse sequences are demonstrated on a model compound, RbNO3, and also applied on β-Ga2O3, a sample with the largest quadrupolar interactions for which isotropic NMR spectra have been obtained to date.

Optimization of adiabatic pulses for pulsed arterial spin labeling at 7 tesla: Comparison with pseudo-continuous arterial spin labeling.

To optimize and evaluate adiabatic pulses for pulsed arterial spin labeling at ultrahigh field 7 tesla. Four common adiabatic inversion pulses, including hyperbolic secant, wideband uniform rate smooth truncation, frequency offset corrected inversion, and time-resampled frequency offset corrected inversion pulses, were optimized based on a custom-defined loss function that included labeling efficiency and inversion band uniformity. The optimized pulses were implemented in flow-sensitive alternating inversion recovery sequences and tested on phantom and 11 healthy volunteers with 2 constraints: 1) specific absorption rate normalized; and 2) equal peak RF amplitude, respectively. A pseudo-continuous arterial spin labeling sequence was implemented for comparison. Quantitative metrics such as perfusion and relative labeling efficiency versus residual tissue signal were calculated. Among the 4 pulses, the wideband uniform rate smooth truncation pulse yielded the lowest loss in simulation and achieved a good balance between labeling efficiency and residual tissue signal from both phantom and in vivo experiments. Wideband uniform rate smooth truncation-pulsed arterial spin labeling showed significantly higher relative labeling efficiency compared to the other sequences (P < .01), whereas the perfusion signal was increased by 40% when the highest amplitude was used. The 4 pulsed arterial spin labeling sequences yielded comparable perfusion signals compared to pseudo-continuous arterial spin labeling but with less than half the specific absorption rate. Optimized wideband uniform rate smooth truncation pulse with the highest amplitude allowed was recommended for 7 tesla pulsed arterial spin labeling.

Cerebrospinal fluid flow in normal beagle dogs analyzed using magnetic resonance imaging.

BackgroundDiseases related to cerebrospinal fluid flow, such as hydrocephalus, syringomyelia, and Chiari malformation, are often found in small dogs. Although studies in human medicine have revealed a correlation with cerebrospinal fluid flow in these diseases by magnetic resonance imaging, there is little information and no standard data for normal dogs.ObjectivesThe purpose of this study was to obtain cerebrospinal fluid flow velocity data from the cerebral aqueduct and subarachnoid space at the foramen magnum in healthy beagle dogs.MethodsSix healthy beagle dogs were used in this experimental study. The dogs underwent phase-contrast and time-spatial labeling inversion pulse magnetic resonance imaging. Flow rate variations in the cerebrospinal fluid were observed using sagittal time-spatial labeling inversion pulse images. The pattern and velocity of cerebrospinal fluid flow were assessed using phase-contrast magnetic resonance imaging within the subarachnoid space at the foramen magnum level and the cerebral aqueduct.ResultsIn the ventral aspect of the subarachnoid space and cerebral aqueduct, the cerebrospinal fluid was characterized by a bidirectional flow throughout the cardiac cycle. The mean ± SD peak velocities through the ventral and dorsal aspects of the subarachnoid space and the cerebral aqueduct were 1.39 ± 0.13, 0.32 ± 0.12, and 0.76 ± 0.43 cm/s, respectively.ConclusionsNoninvasive visualization of cerebrospinal fluid flow movement with magnetic resonance imaging was feasible, and a reference dataset of cerebrospinal fluid flow peak velocities was obtained through the cervical subarachnoid space and cerebral aqueduct in healthy dogs.

Role of Double Inversion Recovery Sequence in Neuro-imaging on 3 Tesla MRI

Double Inversion Recovery (DIR) is a robust sequence designed to suppress fat and water signals using two 180° inversion pulses to produce prominent gray matter contrast with high spatial resolution. It has proven to be more sensitive in delineating white matter signal abnormalities than conventional MR techniques. In our study, the highest image contrast with lesion load was observed using DIR over FLAIR and T2 weighted imaging. DIR is evidently valuable for the detection of demyelinating lesions observed in multiple sclerosis (MS), malignancies, epileptogenic foci, and cortical anomalies. Hence this pictorial review is intended to assess the diagnostic efficacy of DIR modality in clinical Neuro-imaging.

IFEDC-20194285 Phase-controlled Inversion Under High-resolution Pseudo-impedance Curve

Currently, conventional seismic inversions are mostly premised on well data, and there exist uncertainty in seismic inversions in areas with few fixed wells. After years of production practice, well-seismic cooperative inversion under the constraint of pseudo wells is a more effective method for reservoir prediction in areas with few fixed wells and complex phase spread changes. Nevertheless, the selection of locations of conventional pseudo wells only takes into account the spatial distribution of wells but seldom considers the changes of phase spreads; curves of pseudo wells are mostly derived from sparse pulse inversion with relatively low longitudinal resolution, which cannot accurately reflect the vertical and horizontal variation characteristics of the reservoir; there is no effective quality control method to prove the reliability of the curve after selecting the pseudo well. To solve the above-mentioned problems, this thesis acquires pseudo-wave impedance curves with high-frequency components by means of waveform difference inversion, controls quality and optimizes the location of pseudo wells under the guidance of geological results, and performs phase control inversion under the constraint of high-frequency pseudo-wave impedance curves, which greatly enhances the vertical and horizontal resolution of inversion, solves the problem of fine reservoir prediction in areas with few fixed wells, and offers strong support for high-speed and efficient development of oil and gas.

Ultrashort echo time time-spatial labeling inversion pulse magnetic resonance angiography with denoising deep learning reconstruction for the assessment of abdominal visceral arteries.

Current contrast-enhanced magnetic resonance angiography (MRA) and non-contrast-enhanced balanced steady-state free precession (bSSFP) MRA cause susceptibility artifacts from metallic devices in assessing endovascular visceral-artery interventions. The aims of this study are to investigate and compare image quality (IQ) and susceptibility artifacts of three-dimensional (3D) ultrashort echo time (UTE) time-spatial labeling inversion pulse (Time-SLIP) with those of 3D bSSFP Time-SLIP and to assess denoising deep learning reconstruction (dDLR) for the improvement of the signal-to-noise ratio (SNR) in 3D UTE with sparse sampling in phantoms and human subjects. This is a prospective type of study. Pulsatile glycerin-water flow phantom with platinum-tungsten-alloy coil, stainless-steel, nitinol, and cobalt-alloy stents were used. Ten healthy volunteers (seven males) and three patients (two males) were included in this study. 3D UTE Time-SLIP and 3D bSSFP Time-SLIP at 3T were used. The phantom-based study compared the signal-intensity ratio of the device levels (SRdevice ) and distal segments (SRdistal ) to the proximal segments. The volunteer-based study measured SNR, contrast ratio (CR), and IQ. The patient study evaluated local artifacts from metallic devices. Statistical tests included paired t-tests, Wilcoxon-signed rank tests, and Kruskal-Wallis tests. In the phantom-based study, SRdevice was small with UTE Time-SLIP, except the stainless-steel stent. SRdistal was greater (49.1%-90.4%) on bSSFP images than UTE images (-11.1% to 9.6%). Among volunteers, dDLR in UTE images improved SNR (p < 0.05) and IQ (p < 0.05), but CR was unaffected. UTE Time-SLIP showed inferior SNR and IQ than bSSFP Time-SLIP in images with and without dDLR (p < 0.05 for each). However, among patients, UTE Time-SLIP showed reduced metal artifacts compared to bSSFP Time-SLIP. Irrespective of the lower SNR and IQ of 3D UTE Time-SLIP than those of 3D bSSFP Time-SLIP, the former appeared to better depict flow after stenting or coiling. This indicates the potential of 3D UTE Time-SLIP to provide suitable diagnostic images of target vessels. dDLR improved SNR with reducing artifacts related to radial sampling, while maintaining the contrast. LEVEL OF EVIDENCE: 2. TECHNICAL EFFICACY STAGE: 2.

Measuring pulmonary gas exchange using compartment-selective xenon-polarization transfer contrast (XTC) MRI.

To demonstrate the feasibility of generating red blood cell (RBC) and tissue/plasma (TP)-specific gas-phase (GP) depolarization maps using xenon-polarization transfer contrast (XTC) MR imaging. Imaging was performed in three healthy subjects, an asymptomatic smoker, and a chronic obstructive pulmonary disease (COPD) patient. Single-breath XTC data were acquired through a series of three GP images using a 2D multi-slice GRE during a 12 s breath-hold. A series of 8 ms Gaussian inversion pulses spaced 30 ms apart were applied in-between the images to quantify the exchange between the GP and dissolved-phase (DP) compartments. Inversion pulses were either centered on-resonance to generate contrast, or off-resonance to correct for other sources of signal loss. For an alternative scheme, inversions of both RBC and TP resonances were inserted in lieu of off-resonance pulses. Finally, this technique was extended to a multi-breath protocol consistent with tidal breathing, involving 30 consecutive acquisitions. Inversion pulses shifted off-resonance by 20 ppm to mimic the distance between the RBC and TP resonances demonstrated selectivity, and initial GP depolarization maps illustrated stark magnitude and distribution differences between healthy and diseased subjects that were consistent with traditional approaches. The proposed DP-compartment selective XTC MRI technique provides information on gas exchange between all three detectable states of xenon in the lungs and is sufficiently sensitive to indicate differences in lung function between the study subjects. Investigated extensions of this approach to imaging schemes that either minimize breath-hold duration or the overall number of breath-holds open avenues for future research to improve measurement accuracy and patient comfort.

Epidural Oscillating Cardiac-Gated Intracranial Implant Modulates Cerebral Blood Flow.

We have previously reported a method and device capable of manipulating ICP pulsatility while minimally effecting mean ICP. To test the hypothesis that different modulations of the intracranial pressure (ICP) pulse waveform will have a differential effect on cerebral blood flow (CBF). Using an epidural balloon catheter attached to a cardiac-gated oscillating pump, 13 canine subjects underwent ICP waveform manipulation comparing different sequences of oscillation in successive animals. The epidural balloon was implanted unilaterally superior to the Sylvian sulcus. Subjects underwent ICP pulse augmentation, reduction and inversion protocols, directly comparing time segments of system activation and deactivation. ICP and CBF were measured bilaterally along with systemic pressure and heart rate. CBF was measured using both thermal diffusion, and laser doppler probes. The activation of the cardiac-gate balloon implant resulted in an ipsilateral/contralateral ICP pulse amplitude increase with augmentation (217%/202% respectively, P<.0005) and inversion (139%/120%, P<.0005). The observed changes associated with the ICP mean values were smaller, increasing with augmentation (23%/31%, P<.0001) while decreasing with inversion (7%/11%, P=.006/.0003) and reduction (4%/5%, P<.0005). CBF increase was observed for both inversion and reduction protocols (28%/7.4%, P<.0001/P=.006 and 2.4%/1.3%, P<.0001/P=.003), but not the augmentation protocol. The change in CBF was correlated with ICP pulse amplitude and systolic peak changes and not with change in mean ICP or systemic variables (heart rate, arterial blood pressure). Cardiac-gated manipulation of ICP pulsatility allows the study of intracranial pulsatile dynamics and provides a potential means of altering CBF.