Background: The purpose of the work is to investigate the contents of the main nutrients of milk (fat, protein, lactose), as well as the energy value of milk in the process of expression milk using an vacuum electric breast pump and vacuum electric breast pump with compression pulses simultaneously. Method: Surveys were conducted on 9 women 5-8 weeks of lactation. The analysis was carried out in 10 ml samples of milk, which were continuously expression with breast pump «Laktopuls»: only with vacuum stimuli, and simultaneously with vacuum and compression stimuli. The sampling continuity was ensured by a specially developed procedure. Milk analysis in the samples was performed 1-1.5 hours after gland expression on a Miris AB Uppsala, Sweden, human milk analyzer. Finding: The largest changes in milk samples were observed in fat concentration. The fat content in milk increased in each subsequent sample and in the latter samples could exceed its content in the initial samples by 3-4 times. The total amount of fat was about 25% more when milk ejected by breast pump with vacuum and compression stimuli than with vacuum stimulus only. When determining the protein concentration in milk samples, an increase in its concentration was also found during milk ejection, but on 10-20%. In contrast to the dynamics of fat and protein concentrations, the carbohydrate concentration did not change in all women during the milk ejection. Conclusion: A method has been developed that allows analyzing milk nutrients during the continuous removal of milk from the gland. Surveys have shown that ejection of milk with breast pump with vacuum and compression stimuli, increases the amount of fat and protein in breast milk.
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