This study experimentally investigates the combustion characteristics and thermoacoustic instability of two fuels, ethanol and RP-3, at five different blending ratios. The flame images, root temperature of blended fuel flame and pollutant emissions were photographed and tested. The experimental results indicate that high-pressure pulsation amplitude and airflow disturbance can enhance droplets’ evaporation and combustion processes, resulting in a reduction in flame length. Lower evaporation temperature ensures a higher temperature of the flame root while increasing the blend ratio of RP-3 and increasing the global equivalence ratio can increase the temperature level inside the combustion chamber. The root temperature of ethanol flame can reach over 1400 K. Fuel characteristics and burner structure can influence the coupling between pressure pulsation and flame heat release pulsation, leading to differences in the amplitude of combustion instability. The shorter the outlet length of the burner, the wider the stability range of the flame. Moreover, the increase in total burner length results in a shift of the oscillation dominant frequency towards lower frequencies. Emission characteristic curves reveal that high-pressure pulsation amplitude can improve the combustion efficiency of blended RP-3 fuel but are unfavorable for the complete combustion of ethanol. Meanwhile, under the dilution effect of the airflow, the emissions of NOx and CO at the outlet gradually decrease as the airflow increases.