Coffee Pulp Research Articles

Bioactive Potential and Chemical Composition of Coffee By-Products: From Pulp to Silverskin.

Processing the coffee cherry into roasted beans generates a large amount of by-products, which can negatively impact the environment. The aim of this study was to analyze the bioactive potential and chemical composition of different coffee by-products (pulp, husk, parchment, silverskin, defective beans, and green coffee sieving residue) having in mind their bioactive potential for health and well-being. The coffee by-products showed a distinct nutritional composition. The content of ash, protein, fat, and total dietary fiber was significantly higher (p < 0.05) in coffee pulp (10.72% dw), silverskin (16.31% dw), defective beans (8.47% dw), and parchment (94.19% dw), respectively. Defective beans and the sieve residue exhibited a higher content of total phenolics (6.54 and 5.11 g chlorogenic acid eq./100 g dw, respectively) as well as higher DPPH• scavenging activity (3.11 and 2.85 g Trolox eq./100 g, respectively) and ferric-reducing antioxidant power (17.68 and 17.56 g ferrous sulfate eq./100 g dw, respectively). All the coffee by-products considered in this study are sources of caffeine and chlorogenic acids, in particular 5-caffeoylquinic acid (5.36-3787.58 mg/100 g dw, for parchment and defective beans, respectively). Thus, they can be recycled as functional ingredients for food, cosmetic and/or pharmaceutical industries, contributing to the social, economic, and environmental sustainability of the coffee industry.

Exploring the potential of phenolic compounds from the coffee pulp in preventing cellular oxidative stress after in vitro digestion

The coffee pulp, a by-product of the coffee industry, contains a high concentration of phenolic compounds and caffeine. Simulated gastrointestinal digestion may influence these active compounds’ bioaccessibility, bioavailability, and bioactivity. Understanding the impact of the digestive metabolism on the coffee pulp’s phenolic composition and its effect on cellular oxidative stress biomarkers is essential. In this study, we evaluated the influence of in vitro gastrointestinal digestion of the coffee pulp flour (CPF) and extract (CPE) on their phenolic profile, radical scavenging capacity, cellular antioxidant activity, and cytoprotective properties in intestinal epithelial (IEC-6) and hepatic (HepG2) cells. The CPF and the CPE contained a high amount of caffeine and phenolic compounds, predominantly phenolic acids (3′,4′-dihydroxycinnamoylquinic and 3,4-dihydroxybenzoic acids) and flavonoids (3,3′,4′,5,7-pentahydroxyflavone derivatives). Simulated digestion resulted in increased antioxidant capacity, and both the CPF and the CPE demonstrated free radical scavenging abilities even after in vitro digestion. The CPF and the CPE did not induce cytotoxicity in intestinal and hepatic cells, and both matrices exhibited the ability to scavenge intracellular reactive oxygen species. The coffee pulp treatments prevented the decrease of glutathione, thiol groups, and superoxide dismutase and catalase enzymatic activities evoked by tert-butyl hydroperoxide elicitation in IEC-6 and HepG2 cells. Our findings suggest that the coffee pulp could be used as a potent food ingredient for preventing cellular oxidative stress due to its high content of antioxidant compounds.

Microwave-assisted extraction of bioactive compounds from Sarawak Liberica sp. coffee pulp: Statistical optimization and comparison with conventional methods.

Coffea liberica, commonly known as Liberica coffee, is a kind of coffee that originated in Liberia, a West African country. It is considered a less-known coffee bean variety, which accounts for less than 2% of commercially produced coffee worldwide. In this study, the influences of optimization of microwave-assisted extraction (MAE) on the total phenolic content (TPC), total flavonoid content (TFC), and total carbohydrate content (TCC) of bioactive compounds extracted from Sarawak Liberica sp. coffee pulp were studied. Response surface methodology was adopted with a face-centered central composite design to generate 34 responses by taking three microwave parameters into consideration, microwave power (watt), time of irradiation (second), and solvent-to-feed ratio as independent variables. As a result, the findings revealed that optimum extraction conditions were conducted as follows: microwave power of 700 W, time of irradiation of 180 s, and solvent-to-feed ratio of 86.644:1. While under optimal extraction conditions, MAE outperformed conventional maceration extraction in terms of extraction efficiency and had no significant difference (p < .05) with Soxhlet extraction on the extraction of TPC (12.94 ± 2.25 mg GAE/g), TFC (9.84 ± 0.38 mg QE/g), and TCC (876.50 ± 64.15 mg GE/g). Present work advances the usage of Sarawak Liberica sp. coffee for the development of functional products and aids in reducing environmental pollution by utilization of coffee pulp waste.

Physiological response of &amp;#8216;Castillo el Tambo&amp;#8217; coffee plants to biochar and chemical fertilization applications

The fertilizers costs are currently elevated, and it is necessary to evaluate complementary agronomic strategies. Biochar (BC) offers several potential benefits at a relatively low cost. The research aimed was to study the effect of the application of four different doses of biochar (BC) (0, 4, 8, and 16 t ha-1) and four fertilization levels (FL) (0%, 33%, 66% and 100% of the nutritional requirements). We obtained the BC from coffee (Coffea arabica L.) pulp. The selected response variables were: Stomatal conductance (gs), water use efficiency (WUE), root hydraulic conductivity (K), leaf photosynthetic pigments (Chl), and total dry weight (TDW) of coffee ‘Castillo el Tambo’. The experiment was conducted under greenhouse conditions. The results showed that plants with 0 t ha-1 and 0% FL registered low values in the physiological parameters evaluated (gs = 60.5 mmol m-2 s-1; WUE = 3.03 g DM L-1 H2O; Chl = 59.4 atLEAF readings; Kr = 9.7×10-6 kg s-1 MPa-1; TDW = 9.7 g). On the other hand, we recorded a positive effect when ‘Castillo el Tambo’ coffee plants received 8 t ha-1 BC (Kr = 11.8×10-6 kg s-1 MPa-1), especially at fertilization levels of 66% and 100% (gs = 146.7 and 167.5 mmol m-2 s-1; Chl = 72.8 and 66.6 Chl content; WUE = 4.3 and 5.0 g DM L-1 H2O; TDW = 13.6 and 16.0 g, respectively). In conclusion, the use of BC manufactured with coffee pulp, mainly at a dose of 8 t ha-1, can be an alternative to complement the chemical nutrition of coffee plants.

Coffee Pulp: A Sustainable and Affordable Source for Developing Functional Foods

Coffee cultivation in the Department of Cauca, Colombia, is vital for much of the department’s economy. Coffee processing generates waste, such as pulp, which accounts to about 40% of the fresh fruit. Currently, in most cases in Cauca, coffee byproducts are discarded, and, in other cases, the coffee pulp is used for fertilizers, generating environmental problems due to its decomposition. This research aims to design a process for supplying a functional food in the form of an energy bar based on coffee pulp and other components of the region, such as quinoa and panela, helping to mitigate the environmental impact of coffee production. Our research included four phases. First, we determined the study area; in the second phase, we studied an energy bar’s nutritional and physical characteristics. Then, the requirements and specifications of the bar were defined, and the authors documented the process diagram, variables within the process, and the quality plan. Finally, the authors conducted experiments to determine optimal mixing proportions. From the experiment with the mixtures, we found a formulation that satisfies the needs and specifications of the bar which is composed of 50% cereals, 30% panela syrup, and 20% coffee pulp. The selected formulation’s qualitative properties (organoleptic, chemical, and microbial) are acceptable for human consumption and provide high energy content of 365.21 and 291.56 kcal/100 g for the energy bar and coffee pulp raisins, respectively.

Antioxidant activity and physicochemical composition of coffee pulp obtained from three coffee varieties in Aceh, Indonesia

This study aims to analyze the antioxidant activity and physicochemical composition of coffee pulp from the three coffee varieties in Aceh Province. This research is exploratory. The pulp of arabica, robusta, and liberica coffee was sun-dried to produce cascara. The properties of cascara including antioxidants, total soluble solids, total titratable acidity, pH, moisture content, ash content, fat content, protein content, crude fiber, and total phenol were evaluated. The results showed that the pulp of arabica, robusta, and liberica coffee could reduce free radicals using the DPPH method with a very strong intensity, where the IC50 produced was ranged 33.95 to 44.94 ppm (very strong category ≤ 50 ppm). The results of the physicochemical analysis included for total soluble solids (TSS) at 0.83 − 16.00°Brix; total titratable acidity 0.001− 0.160%; pH 4.68 − 5.59; moisture content 67.61−79.49%; ash content 1.39 − 3.74%; fat content 0.3 − 1.01%; protein content 4.08 − 7.88%; crude fiber content 13.79 − 38.51% and total phenol 21.99 − 80.00 mg GAE/100g sample. These results can be the basis for diversifying the utilization of coffee pulp waste into useful products such as functional drinks like cascara and kombucha.

Study on biogas production from coffee wastes and cow-dung fermentation and its use towards reducing emissions from coffee roasting

The production of high-quality coffee for export is an important industry in Indonesia. However, coffee processing produces unavoidable waste and emissions to the environment; 1 kg of cherry beans (CB) needs 3 kg of water for processing (2 kg for CB selection by floating, 1kg for wash-fermentation). One kg CB generates several waste and product streams: cherry skin (44%), beans (20%), parchment (5%), pulp (4%), silverskin (0.5%), and volatiles (26.5%). This study utilized the wastewater from the washed-coffee processing such as fermentation wash water and coffee pulp. The materials were mixed with cow dung from local farms for anaerobic processing in a one-gallon digester. This research took into consideration three variations: cow dung mixed with water in the same amount (1:1); cow dung and fermentation washing wastewater (1:1); and cow dung and pulp (1:1). The resulting biogas was analyzed with gas chromatography for comparison. Biogas yield was used to estimate the potential for supplying heat and electricity for use in the coffee roasting process. Alternative energy sources were also considered in the following combinations: biogas-electricity (solar panels) and LPG-electricity (fossil). A life cycle assessment was performed on scenarios to calculate global warming potential (GWP). This study will be used as a pilot project toward a zero-waste and zero-emission coffee industry. The scenario with the lowest GWP can be used as a reference for the sustainability of coffee production.

Coffee pulp pretreatment methods: A comparative analysis of hydrolysis efficiency

The Vietnamese food industry produces a lot of coffee pulp, which is a valuable and abundant source of agricultural by-products. It contains a lot of cellulose, which can be converted into bioethanol. However, coffee pulp needs an extensive pretreatment to reduce the amount of lignin and hemicellulose while retaining the initial cellulose composition. This study compared several pre-hydrolysis and pre-fermentation pretreatment methods which involved H2SO4, NaOH, microwaves, and white rot fungus Phanerochaete chrysosporium.&#x0D; The hemicellulose dropped by 43.8% after the acidic pretreatment, by 47.1% after the alkaline pretreatment, and by 12.8% after the microbial pretreatment. The lignin contents dropped by 4.2, 76.6, and 50.2% after acidic, alkaline, and microbial pretreatment, respectively. The removal of hemicellulose and lignin in the coffee pulp was much more efficient when two or three of the pretreatment methods were combined. The microwave-assisted acid and alkaline pretreatment was the most efficient method: it removed 71.3% of hemicellulose and 79.2% of lignin. The combined method also had the highest amount of reducing sugars and glucose in hydrolysate. Additionally, concentrations of such yeast inhibitors as 5-hydroxymethyl-2-furaldehyde (HMF) and furfural were 2.11 and 3.37 g/L, respectively.&#x0D; The acid pretreatment was effective only in removing hemicellulose while the alkaline pretreatment was effective in lignin removal; the fungal pretreatment had low results for both hemicellulose and lignin removals. Therefore, the combined pretreatment method was found optimal for coffee pulp.

Restoring Ampicillin Sensitivity in Multidrug-Resistant Escherichia coli Following Treatment in Combination with Coffee Pulp Extracts.

Escherichia coli, particularly multidrug-resistant (MDR) strains, is a serious cause of healthcare-associated infections. Development of novel antimicrobial agents or restoration of drug efficiency is required to treat MDR bacteria, and the use of natural products to solve this problem is promising. We investigated the antimicrobial activity of dried green coffee (DGC) beans, coffee pulp (CP), and arabica leaf (AL) crude extracts against 28 isolated MDR E. coli strains and restoration of ampicillin (AMP) efficiency with a combination test. DGC, CP, and AL extracts were effective against all 28 strains, with a minimum inhibitory concentration (MIC) of 12.5-50 mg/ml and minimum bactericidal concentration of 25-100 mg/ml. The CP-AMP combination was more effective than CP or AMP alone, with a fractional inhibitory concentration index value of 0.01. In the combination, the MIC of CP was 0.2 mg/ml (compared to 25 mg/ml of CP alone) and that of AMP was 0.1 mg/ml (compared to 50 mg/ml of AMP alone), or a 125-fold and 500-fold reduction, respectively, against 13-drug resistant MDR E. coli strains. Time-kill kinetics showed that the bactericidal effect of the CP-AMP combination occurred within 3 h through disruption of membrane permeability and biofilm eradication, as verified by scanning electron microscopy. This is the first report indicating that CP-AMP combination therapy could be employed to treat MDR E. coli by repurposing AMP.

Substitution Effect of Rice Bran with Coffee Pulp Meal on Feed Efficiency and Percentages of Viscera Organs of Broilers

Research on animal feed has often focused on finding alternative feed ingredients to replace conventional ones in order to reduce feed costs. By-products from processing crops and food products have received much attention as feed alternatives because of their consistent and mass production, one of them is a waste of coffee. This research was conducted to evaluate the substitution effect of rice bran with coffee pulp meal on feed efficiency and viscera organs of broiler. The 100 broilers (Hubbard strain) and placed randomly into 4 treatments (R0 = ration with rice bran 21% + 0% coffee pulp meal; R1 = ration with 14% rice bran + 7% coffee pulp meal; R2 = ration with 7% rice bran + 14% coffee pulp meal; R3 = ration with 0% rice bran + 21% coffee pulp meal and each treatment consisted of 5 replications, five broilers each. The broilers were arranged by completely randomized design; a Tukey test was used to compare mean different of the treatments. Substitution effect of Rice Bran with Coffee Pulp Meal on ration had significant effect on decreasing feed efficiency and significantly increase the weight percentage of heart, gizzard, pancreas, and intestine (P&lt;0.05) but had no significant on the weight percentage of caecum in broiler (P&gt;0.05) Tukey test showed that the higher proportion of coffee hull meal the feed consumption and body gain non-significant to R0, R1 and R2. On the other hand feed efficiency of R3 significantly lower compared to R0, R1and R2. The higher the percentage of liver, gizzard, pancreas and intestine weight. However, there was no significant effect on caecum weight percentage. The conclution was: Utilization coffee pulp meal had significant effect on decreasing feed efficiency and significantly increase the weight percentage of heart, gizzard, pancreas, and intestine

Green solvents for the selective extraction of bioactive compounds from by-products of the coffee production chain

Approximately 15–20 million tonnes of by-products were generated globally in the coffee production chain during the 2020/21 season. The current uses for these by-products are inadequate or contribute to environmental degradation. This work reports the screening of hydrophilic and hydrophobic solvents, including deep eutectic solvents (DES), for their ability to extract the valuable metabolites caffeine and chlorogenic acid (markers in commercial extraction) from four coffee-related by-products. Water was the best solvent for recovering caffeine and chlorogenic acid from defective green coffee beans, while all hydrophilic DES surpassed water in the extraction of chlorogenic acid from coffee pulp. For the two remaining by-products, at least one DES was as good or better at extracting the caffeine or chlorogenic acid compared to water. The array of hydrophobic solvents tested here showed different selectivities, allowing the annotation of 12 compounds that have not been previously reported in coffee by-products. These results demonstrate the ability to obtain a new and controllable range of extracts from coffee by-products using green and functional solvents.

Value-Added Products from Coffee Waste: A Review.

Coffee waste is often viewed as a problem, but it can be converted into value-added products if managed with clean technologies and long-term waste management strategies. Several compounds, including lipids, lignin, cellulose and hemicelluloses, tannins, antioxidants, caffeine, polyphenols, carotenoids, flavonoids, and biofuel can be extracted or produced through recycling, recovery, or energy valorization. In this review, we will discuss the potential uses of by-products generated from the waste derived from coffee production, including coffee leaves and flowers from cultivation; coffee pulps, husks, and silverskin from coffee processing; and spent coffee grounds (SCGs) from post-consumption. The full utilization of these coffee by-products can be achieved by establishing suitable infrastructure and building networks between scientists, business organizations, and policymakers, thus reducing the economic and environmental burdens of coffee processing in a sustainable manner.

The Application of Coffee Pulp Biochar Improves the Physical, Chemical, and Biological Characteristics of Soil for Coffee Cultivation

A field experiment was conducted for 2 years (2019 and 2020) to determine the response to the application of Biochar (BC) obtained from the coffee pulp in combination with chemical fertilization (CF) in coffee trees. The established treatments were the edaphic application of different doses of BC (0, 4, 8, and 16 t ha−1) and levels of CF (0, 33, 66, and 100% of the nutritional requirements) on the physical (bulk density (ρa), stable aggregates), chemical (nutrient availability), and biological (respiration) properties of soil from a coffee crop. Regarding the physical properties, it was mainly observed that BC doses of 8 or 16 t ha−1 reduced ρa (0.82 and 0.83 g cm−3, respectively) and increased the aggregation status (96.5% and 96.84%, respectively) in comparison to 0 t ha−1 in 2020. The chemical properties showed that the application of 16 t ha−1 BC decreased about 60% of the exchangeable acidity (EA) compared to the control (0 t ha−1) whereas the pH (4.96 and 4.92) and organic carbon (OC) (4.41 and 4.59) were higher than in the control soil (EA: 0.58 and 0.54 meq/100 g; pH: 4.63 and 4.55; OC: 4.17 and 4.32% in 2019 and 2020, respectively). Soil respiration (biological property) strongly increased (around 50–60%) with the combination of 66% CF and BC doses between 8 and 16 t ha−1 in both years. Doses between 8 and 16 t ha−1 BC of the coffee pulp can improve the quality of soils for coffee cultivation and provide an alternative and more sustainable amendment that may help reduce chemical fertilization.

Coffee Pulp Biomass Utilization on Coffee Production and Its Impact on Energy Saving, CO2 Emission Reduction, and Economic Value Added to Promote Green Lean Practice in Agriculture Production

The global market’s sustainability demand for coffee as a result of environmental concerns has influenced coffee producers to practice green coffee production. The efforts to improve the environmental performance of coffee production should also consider the other sustainability aspects: energy and economics. Using a green fertilizer from agricultural biomass can lower carbon dioxide (CO2) emissions since the cultivation process, which is directly impacted by fertilizer use, has been identified as an environmental damage hotspot for coffee production. This study aims to determine the impact of coffee pulp biomass utilization on coffee production in terms of energy savings, CO2 emission reduction, and economic value added. The methodologies used were environmental Life Cycle Assessment, energy requirement analysis, life cycle costing, and eco-efficiency analysis. The study findings showed that using coffee pulp biomass in coffee cultivation impacted the energy savings, environmental damage reduction, and increased economic value added. Applying coffee pulp biomass can potentially reduce 39–87% of cumulative energy demand, 49.69–72% of CO2 emissions, and 6–26% of the economic value-added increase. Moreover, coffee pulp utilization as a fertilizer is recommended to be applied broadly to promote sustainable coffee production according to its beneficial impact. This study provided that scientific information farmers need to apply green fertilizers in coffee production.

Effects of roasting on the chemical compositions, color, aroma, microstructure, and the kinetics of changes in coffee pulp.

Roasting is a critical process that affects the quality attributes of coffee beans; however, how roasting conditions affect the physical, chemical, biological, and organoleptic changes of coffee pulp needs more research. In the present study, we investigated the effects of roasting temperatures and times on chemical compositions and quality attributes of coffee pulp. The results showed that the contents of total soluble sugar (TSS) and free amino acid (FAA) followed a temporal pattern of first increasing and then decreasing under the roasting temperatures between 100 and 160°C. Total phenolic content (TPC) and antioxidant activity of coffee pulp significantly (p<0.05) increased after roasting, reaching the maximum values of 83.09 mg gallic acid equivalent(GAE)/g and 360.45 µM 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid(Trolox)/g, respectively, when coffee pulp was roasted at 160°C for 18min. Drying rates of coffee pulp fitted the Logarithmic kinetic model, while color (L*, a*, and b*) changes and 5-caffeoylquinic acid degradation followed the first-order kinetic model. Electronic nose analysis showed that the main aroma compounds of the coffee pulp are sulfur-containing organics that were reduced with the extended roasting time. Scanning electronic microscopy analysis presented the loosened, shrunk, and cracked microstructure on the surface of the roasted coffee pulp, which might contribute to the increased TSS, FAA, TPC, and antioxidant activity of coffee pulp roasted under specific conditions. In conclusion, our research provides valuable information for preparing high-quality coffee pulp tea. PRACTICAL APPLICATION: This article investigates the effects of roasting on the chemical composition, color, flavor, microstructure, and the kinetics of changes in the moisture, color, and 5-caffeoylquinic acid (5-CQA) of the coffee pulp. We have found that high-temperature and short-time roasting helps retain the total phenolic contents, antioxidant activity, and aroma. The drying kinetic fits the Logarithmic model, and the changes in color and 5-CQA fit the first-order kinetic model. This study provides meaningful information for preparing coffee pulp tea with high-quality attributes and antioxidant activity.

Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp

Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates were obtained, and thirty-three isolates showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by isolates C12, identified as Leclercia adecarboxylata based on 16S-rRNA gene sequence analysis. SDS-PAGE of Leclercia adecarboxylata C12 cellulase revealed two bands with a molecular mass of 95.49 and 81.28 kDa, respectively. Activity gel analysis showed the cellulolytic ability of Leclercia adecarboxylata C12 cellulase by clear zone formation. The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. The activity of CMCase was stimulated by the presence of K+, Ca2+, Mg2+, and Fe3+, while SDS and EDTA reduced its activity. The current study shows that the thermostable-alkalophilic cellulase produced by Leclercia adecarboxylata C12 is very promising for industrial applications.

Chlorogenic acid rich in coffee pulp extract suppresses inflammatory status by inhibiting the p38, MAPK, and NF-κB pathways

Coffee pulp (CP) is a coffee byproduct that contains various active ingredients, namely, chlorogenic acid (CGA) and caffeine. These active compounds show several benefits, including antihyperlipidemia, antioxidants, and anti-inflammation. However, the anti-inflammatory properties of Coffea pulp extract (CPE) are unknown. This work determined the impact of CPE on lipopolysaccharide (LPS)-activated murine macrophage cells and the molecular mechanism behind this action. RAW 264.7 cells were exposed to varying doses of CPE with or without LPS. Inflammatory markers and their mechanism were studied. CPE therapy has been shown to suppress the synthesis of inflammatory cytokines and mediators, namely, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), IL-1β, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and nitric oxide (NO), as well as prostaglandin E2 (PGE2). Finally, CPE inactivated the nuclear factor-kappa B (NF-κB) and MAPK signaling pathways. Consequently, CPE might be used as a nutraceutical to treat inflammation and its related disorders.

Effects of the inclusion of coffee pulp silage in the diet on the performance and profitability of crossbred milk cows in the middle tropics

The objective of this study was to evaluate the inclusion of coffee pulp silage (CPS) on the performance and profitability of crossbred cows in the middle tropics. The research took place at the Villa Marina Experimental Farm of the University of Pamplona, Pamplonita, Colombia. Four lactating multiparous bovines with a body weight of 380 ± 10 kg, 6 ± 0.03 years of age and a body condition of 3.8 on a scale of 1 to 5. The animals were distributed in a balanced 4 × 4 Latin square for residual effect. The experiment consisted of four subperiods of 21 days each, being the first 14 days of adaptation to the experimental diet and 7 days for taking samples. The base diet consisted of Pennisetum sp. forage, concentrated in a proportion of 0.2% of body weight, water, and mineralized salt at will. The inclusion of the CPS was included in levels of 4, 8, and 12% of the dry matter (DM) intake. DM intake parameters, nutrient digestibility, milk production and composition, blood parameters, and economic analysis were evaluated. Treatments were compared using orthogonal contrasts; contrasts were constructed in order to evaluate the effects of inclusion of CPS, and the linear and quadratic effects of level inclusion in the DM of the diet. For the variables which did not present inclusion of CPS effect but a linear or quadratic effect was significant, a Dunnett’s test was performed to identify whether a supplemented treatment differed from the control. Significance was considered at P < 0.05 and tendencies when 0.05 < P ≤ 0.10. The inclusion of CPS in the diet increases (P < 0.05) the voluntary intake in kg day−1 of DM, organic matter (OM), CP, ethereal extract (EE), non-fiber carbohydrates (NFC), digested OM (DM), neutral detergent fiber corrected for ash and protein (NDFap), DNDF and dietary NTD concentration, the total digestibility of DM and OM, and dietary DOM content. The production and chemical composition of milk was not affected (P > 0.10) by the inclusion of EPC in the diet. The inclusion of CPS increased (P < 0.05) the concentration of albumin and globulins in the blood. The inclusion of CPS in the diet improved the productive efficiency of dairy cattle. The inclusion of 4% CPS in the DM of the diet is a nutritional strategy, which improves the nutritional characteristics and the concentration of albumin and globulins in the blood of crossed dairy cows in the middle tropics and benefit: cost ratio without affecting milk production.

Advanced extraction of caffeine and polyphenols from coffee pulp: Comparison of conventional and ultrasound-assisted methods

Two extraction methods of caffeine and total polyphenols from coffee pulp waste were evaluated using water as solvent: conventional solid-liquid extraction (CSLE) and ultrasound-assisted extraction (USAE). A central rotary experimental design was applied in each case, after evaluating the effect of the substrate:solvent ratio. The highest concentration of caffeine in the extractant using CSLE was found to be 49.9 ± 1.3 mg/L (equivalent to 3.7 ± 0.1 g/kg pulp, dry basis (d.b.)), after 5.5 min at 96 °C, and at a substrate:solvent ratio of 1:10 w/v. In parallel, the concentration of total polyphenols reached 128.3 ± 4.3 mg gallic acid equivalent (GAE)/L (9.7 ± 0.3 g/kg) at 75 °C (equal time and ratio). USAE was found to be also affected by power, leading to 206.6 ± 0.1 mg caffeine/L and 164.9 ± 5.2 mg GAE/L (15.6 ± 0.3 g/kg and 12.4 ± 0.2 g/kg, respectively) (396 W, 5.5 min, 75–90 °C). These results indicate extraction yields as high as 85%, at a markedly lower energy requirement using USAE. Consequently, USAE using water as a solvent might be a promising and innovative alternative to improve the extraction performance of highly demanded biocompounds from coffee pulp waste, with the consequent environmental and social benefits within a markedly relevant agro-industrial sector.

Increase in Polyphenolic Substances from Fermented Robusta Coffee Pulp (Coffea canephora L.) by Using Indigenous Actinomycetes

The fermentation of the coffee fruit processing into coffee beans leaves the fermented coffee pulp which still contains polyphenol secondary metabolites. This study aimed to analyze the total flavonoid content (TFC) and total phenolic content (TPC) of fermented robusta coffee pulp (Coffea canephora L.) by using indigenous Actinomycetes. The ability of fermented extracts to inhibit free radical DPPH (2,2-diphenyl-1-picrylhydrazyl) also were done. In this study, coffee cherries were fermented in the solid-state fermentation cultivation (SSF) using isolates HJ4.5b, P2b(b).3 and P2b(b).18 which in previous studies were reported to have cellulolytic and xylanolytic activities. Determination of molecular identification base on 16S rRNA gen showed the isolates HJ4.5b and P2b(b).3 have similarities to the genus Streptomycetes, while P2b(b).18 has a homologous base arrangement with a rare actinomycetes genus Micromonospora. Fermentation using P2b(b).3 on the 9th day of fermentation indicated the highest percentage increase in TFC (295.54%) with IC50 18.41 µl/ml and having an antioxidant activity index (AAI) value of 2.14 which was included in the very strong antioxidant activity category.