Since the removal of resin-luted all-ceramic restorations is a challenge, the use of Er:YAG lasers has become popular. The aim of this study was to determine the removal time of monolithic lithium disilicate crowns in different thicknesses and heat transmission to pulp using Er:YAG laser.Forty-five full-coverage monolithic lithium disilicate crowns in 1 mm (n = 15), 1.5 mm (n = 15), and mixed thickness (n = 15) were resin luted on relevant extracted human maxillary first premolars and subjected to Er:YAG laser irradiation for crown removal after 24 h. Laser parameters for each thickness, respectively, were 5 W, 5.6 W, and 5.9 W (10 Hz). The removal time and temperature change values were recorded for each sample. The statistical evaluations were performed using one-way ANOVA variance and post hoc Duncan and Tamhane's T2 tests (p < 0.05), and Pearson correlation coefficient was used to examine the significance within each group and without group discrimination.All crowns were laser-debonded successfully. The removal time (min:s) at the succeeding laser parameter for each group is as follows: between 2:30 and 4:45 at 5 W power for 1-mm samples, between 5:00 and 11:15 at 5.9 W power for 1.5-mm samples, and between 8:45 and 15:00 at 5.9 W power for samples in mixed thickness. Moreover, it was observed that the temperature changes in the pulp chamber did not exceed the critical value of 5.5 °C for any sample.Er:YAG laser irradiation is an effective and safe method for removal of all-ceramic crowns when appropriate laser parameters are used according to thickness.