Climate change, linked with an increase in temperature, contributes to the possibility growing of non-typical and lesser-known vegetable species in the Slovak Republic, including okra. Seven okra cultivars were tested as part of this study, which focused on the basic yield parameters, antioxidant activity and total polyphenol content in okra fruits. The statistical analysis of obtained results revealed significant differences in all monitored parameters of okra. The selection of “plastic” crop cultivars that can produce a steadier yield in each growing year is required due to the significant climate variations between growing seasons. In this regard, the okra cultivar ‘Baby Bubba’ can be recommended. The results also showed the high antioxidant activity and polyphenol content of okra pulp and seeds. Okra has a higher nutritional quality than other vegetable species frequently grown in the Slovak Republic. Okra cultivars ‘Burgundy’, ‘Pure Luck F1′ and ‘Jing Orange’ can be suggested from a nutritional perspective. Finally, it is possible to state that okra cultivars showed good yield potential and nutritional quality compared to the studies realized in countries in which okra is known and commonly used by the human population.