Pullulan Nanofibers Research Articles

Advanced pullulan nanofibers reinforced by cellulose fibrils as drug carriers for salicylic acid.

The goal of the current work is to showcase the synthesis of homogeneous pullulan nanofibers that are strengthened by the addition of cellulose nanofibrils (CNF). One of the main difficulties this study faced was determining the ideal water/organic solvent ratio for the electrospinning process, which would allow for the maximum reduction in the amount of organic solvent (DMF or DMSO) needed. The rheological behavior of electrospinning solutions was modulated by varying both, the pullulan concentration and solvent system composition. The amount of CNF in the composition significantly affects the properties of the created nanofibers. When the CNF content increased from 1 % to 5 %, the diameter of the fibers decreases from 284 nm to 90 nm. This is attributed to the enhanced conductivity and surface charge density of the solution jet. The as prepared nanofibres can hold a variety of drugs and can be used to create novel formulations for various biomedical purposes. The nanofibres were tested for salicylic acid incorporation and release. Drug release exhibited zero-order kinetics, suggesting that the concentration remained unaffected by the rate of release. Furthermore, the nanofibres demonstrated remarkable antibacterial activity against both Gram-positive and Gram-negative bacteria.

Effect of high amylose starch on the incorporation of thymol into electrospun pullulan nanofibers

Effect of high amylose starch on the incorporation of thymol into electrospun pullulan nanofibers

Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem Cells

This study investigated the development of Pullulan/Collagen nanofiber scaffolds integrated with mesenchymal stem cells (MSCs) to enhance chronic wound healing. The combination of these biopolymers aims to optimize the scaffold properties for cell growth, viability, and tissue regeneration. Materials and Methods: Pullulan, Collagen, and Pullulan/Collagen composite nanofibers were fabricated using electrospinning. The fibers were characterized using scanning electron microscopy (SEM) to determine the fiber diameter, and Fourier-transform infrared spectroscopy (FTIR) was employed to assess the molecular interactions. Cell viability was evaluated using MSCs cultured on the scaffolds and apoptosis assays were conducted to assess cell health. Distilled water was used as the solvent to maximize biocompatibility. Results: SEM analysis revealed that Pullulan nanofibers exhibited a larger average diameter (274 ± 20 nm) compared to Collagen fibers (167.03 ± 40.04 nm), while the Pullulan/Collagen composite fibers averaged 280 ± 102 nm. FTIR confirmed the molecular interactions between Pullulan and Collagen. Regarding biocompatibility, the Pullulan/Collagen scaffold demonstrated superior cell viability at 99% compared to 91% for Pullulan alone. Apoptosis assays indicated significantly lower necrosis rates for the composite scaffold (1.29%) than for the Pullulan-only scaffolds (2.35%). Conclusion: The use of distilled water as a solvent played a critical role in increasing cell viability and facilitating healthy proliferation of MSCs without cellular damage. Additionally, the reduced platelet activation and macrophage activity (0.75-fold for both) further supported the biocompatibility of the Pullulan/Collagen scaffold, demonstrating its potential for tissue engineering and chronic wound healing applications.

Encapsulation of Essential Oil-Cyclodextrin Inclusion Complexes in Electrospun Pullulan Nanofibers: Enhanced Storage Stability and Antibacterial Property for Geraniol and Linalool

Encapsulation of Essential Oil-Cyclodextrin Inclusion Complexes in Electrospun Pullulan Nanofibers: Enhanced Storage Stability and Antibacterial Property for Geraniol and Linalool

Preparation of Eudragit S100-pullulan/hydroxypropyl-β-cyclodextrin complex-Eudragit S100 multilayer nanofiber film for resveratrol colon delivery

Cyclodextrin-based electrospun nanofibers are promising for encapsulating and preserving unstable compounds, but quick dissolution of certain nanofibers hinders their delivery application. In this study, hydroxypropyl-β-cyclodextrin (HPβCD) was used as an effective carrier of resveratrol (RSV) to obtain the RSV/HPβCD inclusion complex (HPIC), which was then incorporated into pullulan nanofibers. For enhancement of RSV release toward colon target, multilayer structure with a pullulan/HPIC film sandwiched between two layers of hydrophobic Eudragit S100 (ES100) nanofibers was employed. The relationship between the superiority of the ES100-pullulan/HPIC-ES100 film and its multilayer structure was verified. The intimate interactions of hydrogen bonds between two adjacent layers enhanced thermal stability, and the hydrophobic outer layers improved water contact resistance. According to release results, multilayer films also showed excellent colon-targeted delivery property and approximately 78.58 % of RSV was observed to release in colon stage. In terms of release mechanism, complex mechanism best described RSV colonic release. Additionally, ES100-pullulan/HPIC-ES100 multilayer films performed higher encapsulation efficiency when compared to the structures without HPIC, which further increased the antioxidant activity and total release amount of RSV. These results suggest a promising strategy for designing safe colonic delivery systems based on multilayer and HPIC structures with superior preservation for RSV.

Fabrication and characterization of progesterone loaded pullulan nanofibers for controlled release

Progesterone is a commonly used drug in humans and livestock that possesses huge therapeutic potential in the regulation of pregnancy and fertility in females. The hydrophobicity and need for prolonged therapy of progesterone restrict the exploitation of its therapeutic potential to the fullest. Recently, polymeric nanofibers have evolved as interesting drug carriers, especially for hydrophobic drugs. The present study aimed to encapsulate the hydrophobic drug progesterone in pullulan by electrospinning for controlled delivery. The progesterone was successfully incorporated in pullulan nanofibers and the mean fiber diameter ranged from 68.68 ± 9.71 to 123.12 ± 17.41 nm. Fourier transform infrared spectroscopy revealed the interaction of the drug with the polymer. The zeta potential of progesterone loaded pullulan nanofibers ranged from 0.4 ± 0.01 to −0.5 ± 0.01 indicating suitability for transmucosal delivery. The hydrodynamic diameter of progesterone loaded pullulan nanofibers were significantly (p < 0.05) lesser than the pullulan nanofibers which may promote better drug adsorption and permeation. The cumulative drug release profile indicated prolonged release of progesterone for 7 days from pullulan nanofibers. The kinetic modelling of the drug release revealed Fickian diffusion of progesterone from the polymeric matrix. Cytotoxicity assay revealed optimal survivability of Baby Hamster Kidney cells (60.51 ± 5.81 to 72.39 ± 0.53 %) on exposure to progesterone loaded pullulan nanofibers ensuring biocompatibility. The present study presents process optimization for successful electro-entrapment of progesterone in biopolymer pullulan and its characterization suggesting the potential of progesterone loaded pullulan nanofibers for controlled delivery.

Electrospun starch-based nanofiber mats for odor adsorption of oyster peptides: Recyclability and structural characterization

The off-odor of seafood, which negatively affected its flavor and quality, was removed mainly by non-recyclable liquid-phase deodorization methods at present. In this work, octenylsuccinylated starch (OSS)-pullulan (PUL) nanofiber mats were regenerated after physical adsorption and thermal desorption of off-odor compounds in oyster peptides. The mat structure was characterized and the adsorption process was investigated. The regenerated nanofiber mats maintained a favorable appearance and microscopic morphology even after 25 adsorption-desorption cycles, with a reduction in average fiber diameter and average pore area, an increase in hydrophobicity and alterations in surface groups. Their adsorption rate first decreased and then stabilized as the number of cycles increased and was as high as 81.76% after 25 cycles. Correlation analysis revealed that the adsorption efficiency of regenerated nanofiber mats was related to their microstructure, surface groups and hydrophobicity. These findings could provide a theoretical basis for odor removal by electrospun nanofiber mats and their recycling.

Development of antifungal electrospun nanofiber mats containing Meyerozyma caribbica

To mitigate vegetable and fruit loss caused by post-harvest fungal diseases, polymeric antifungal coatings encapsulating biocontrol yeasts offer a sustainable alternative. Nanofibers derived from pullulan, cashew gum, FucoPol and poly (ethylene oxide) (PEO), were electrospun and loaded with Meyerozyma caribbica (GenBank ID: JQ398674). The morphological, thermal and chemical properties of cashew gum (CG:PEO), FucoPol (FP:PEO), and pullulan nanofibers were characterized. The viability of M. caribbica within the fibers and their in vitro antifungal activity against six phytopathogens were assessed. Morphological analysis exhibited the presence of nanofibers encapsulating M. caribbica. ATR-FTIR spectroscopy identified the absence of interactions between the yeast and polymers. Fibers containing M. caribbica exhibited in vitro fungistatic effects on spore germination. Pullulan nanofibers showed the highest M. caribbica viability and the highest percentage of growth inhibition against the evaluated fungi. These promising nanofibers encapsulating biocontrol yeasts could be used as edible coatings or agricultural aids, which offer an alternative for post-harvest treatment to control fungal diseases, reducing global food loss.

J-aggregation-induced photoluminescence in electrospun pullulan/ 2-(2-fluorophenyl)-3-hydroxy-4H-chromen-4-one composite mat for photonic applications

The nonfluorescent pullulan matrix was fabricated via an electrospinning technique with intriguing dual emitting 2-(2-fluorophenyl)-3-hydroxy-4H-chromen-4-one as a dopant. The single crystal X-ray diffraction data of the dopant revealed the orientation of the molecules in the crystal lattice. The reduced diameter of the pullulan nanofiber from 328 nm to 32 nm upon fabrication was attributed to the improved crystallinity. The higher moisture resistance of the pullulan/dopant composite nanofiber mat was evidenced by an enhanced water contact angle from 29° to 65.9°. The pullulan/dopant composite nanofiber mat exhibited a bathochromic shift in absorption profile from 250 nm to 296 nm. The subsequent dual emission at 426 nm (Blue) and 532 nm (Green) was accompanied by the large Stoke's shift value of 90–236 nm. The redshift of the emission profile in the pullulan/dopant composite mat from 400 nm to 426 nm could be due to the J-aggregation of the dopant molecules in the pullulan matrix. The band gap of the composite nanofiber mat ranged from 2.41 to 2.91. The optimum quantum yield of 32.1–36.72% and enhanced refractive index value suggested the potential application of fabricated pullulan mat as a dual emissive layer in organic light emitting diode (OLED) material which could be an alternative option for multilayer emissive material in OLED device architecture.

Development and characterisation of MMT-reinforced polyacrylonitrile-pullulan nanofibers for controlled permeation of isotretinoin

Drug nanofibers play a crucial role in ameliorating therapeutic effects, reducing toxicity, and increasing the bioavailability of drugs. This study was aimed at the fabrication of isotretinoin-loaded MMT-reinforced bi-polymeric (PAN/PU) nanofibers with varying concentrations of isotretinoin. In this study, montmorillonite (MMT)-reinforced cross-linked polyacrylonitrile and pullulan nanofibers combined with varying amounts of isotretinoin were fabricated through an electrospinning approach and investigated for their drug permeation potential. PAN-PU nanofibers were successfully integrated with the isotretinoin. The incorporation of isotretinoin into the nanofibrous structure was confirmed by FTIR and XRD. TGA study indicated the stability of the fabricated nanoparticles. The SEM results showed the beaded and smooth morphology of nanofibers. Formulation with a higher drug concentration had a non-significantly (p > 0.05) higher swelling ratio. Drug-loaded polymeric nanofiber erodes at a slower rate as compared to drug-free nanofibers. The ex-vivo permeation study of nanofibers revealed that the drug was not released all at once, but rather gradually and consistently over the period of 24 h, indicating a controlled release of the drug. In addition, the drug concentration in the nanofibers affected the permeation of the drug. According to the findings, isotretinoin-loaded MMT-reinforced bi-polymeric (PAN/PU) nanofibers with varying concentrations of isotretinoin were successfully fabricated. The fabricated nanofibers (PAN/PU) showed a promising potential for controlled permeation of drugs through rabbit skin.

Multilayer polycaprolactone - pullulan nanofiber mats incorporated with the antimicrobial palindromic peptide LfcinB (21-25)Pal as a potential application in active packaging

Food losses are generated by microbial contamination due to the lack of packaging that not only functions as a barrier but releases compounds against such organisms. Electrospinning was used to develop multilayer nanofiber mats using poly-(ε-caprolactone) (PCL) in the outer layer and encapsulating the peptide LfcinB (21-25)Pal in an inner pullulan (PUL) layer. The nanofiber mats were characterized by Fourier Transformed Infrared Spectroscopy (FTIR). FTIR spectra showed the presence of the peptide in PUL nanofiber mats, the presence of both polymers in multilayer nanofiber mats, and the absence of chemical changes. In addition, a High Performance Liquid Chromatography (HPLC) was performed for peptide quantification, where an encapsulation efficiency of 65 % in the nanofiber mat was observed, and a maximum load of 50 mg peptide / g PUL. The antioxidant capacity of the peptide, evaluated through the inhibition of radical DPPH, showed an antiradical activity of 5.21 × 10−4 mg ± 1.12 × 10−5 gallic acid/mg nanofiber mat. Finally, the antimicrobial activity showed a minimum inhibitory concentration of 15 ± 0.01 μM against Escherichia coli. In conclusion, the multilayer nanofiber mat can potentially be used as part of active packaging to prolong the shelf life of foodstuff susceptible to microbial contamination.

X-aggregation-induced emission in newly fabricated Pullulan/3-Hydroxyflavone derivative composite electrospun nanofiber mat for optical applications

A fascinating optically important 3-Hydroxyflavone derivative 2-(2,5-dimethoxyphenyl)-3‑hydroxy-4H-chromen-4-one was synthesized and its single-crystal structure was studied. This compound was used as a dopant and incorporated into the pullulan matrix by electrospinning technique. Functionalized pullulan nanofiber mat was found to possess dopant molecules organized in an X-aggregated fashion accounting for the twisted structure of the dopant molecules. The diameter of the nanofiber mat was reduced from 250 nm to 120 nm upon fabrication with dopant due to the nanofiber mat's increased crystallinity. Dopant molecules imparted absorption and emission properties into the fabricated nanofiber mat and turned it to be highly fluorescent triggering yellowish-green emission with a large Stokes shift of 193 nm due to X-aggregation. The improved water resistance of the fabricated pullulan nanofiber mat was witnessed by an increased water contact angle from 27.3° to 62.5° due to the incorporation of hydrophobic dopant molecules. The thermal and mechanical stability of the fabricated nanofiber mat was improved upon fabrication. The predicted absolute quantum yield was obtained in the 34.1- 40.3% range. The optimum indirect optical band gap of 2.25–2.50 eV and refractive index of 2.1–3.4 for the designed nanofiber mat suggested the potential application as OLED material.

Delivery of streptomycin to the rat colon by use of electrospun nanofibers

Drug-loaded electrospun nanofibers are potential drug carrier systems that may optimize disease treatment while reducing the impact on commensal microbes. The feasibility of streptomycin-loaded pullulan nanofibers fabricated from a green electrospinning procedure using water as the solvent was assessed. We conducted a rat study including a group treated with streptomycin-loaded nanofibers (STR-F, n = 5), a group treated with similar concentrations of streptomycin in the drinking water (STR-W, n = 5), and a non-treated control group (CTR, n = 5). Streptomycin was successfully loaded into nanofibers and delivered by this vehicle, which minimized the quantity of the drug released in the ileal compartment of the gut. Ingested streptomycin-resistant E. coli colonized of up to 106 CFU/g feces, revealing a selective effect of streptomycin even when given in the low amounts allowed by the nanofiber-based delivery. 16S amplicon sequencing of the indigenous microbiota revealed differential effects in the three groups. An increase of Peptostreptococcaceae in the cecum of STR-F animals may indicate that the fermentation of nanofibers directly or indirectly promoted growth of bacteria within this family. Our results elucidate relevant properties of electrospun nanofibers as a novel vehicle for delivery of antimicrobials to the large intestine.

Effect of amylose content on the preparation for carboxymethyl starch/pullulan electrospun nanofibers and their properties as encapsulants of thymol

We proposed a green approach for the preparation of Carboxymethyl starches (CM-starches)/pullulan (PUL) nanofibers and explored their potential properties as encapsulates of thymol. CM-starches from high-amylose (CM-HAS), normal (CM-NCS), and high-amylopectin corn starch (CM-WCS) were synthesized and studied for their spinnability, yet non-fibers could be obtained from these CM-starches. The 20% of PUL was further used to obtain the series of CM-starches/PUL nanofibers from varying CM-starches concentrations. Amylose molecule with efficient molecular entanglement as well as its good solution properties was proved to enhance the electrospinning process with the PUL. Furthermore, the pre-complex of CM-starches-thymol was incorporated in PUL nanofibers using the electrospinning technique. The thymol was successfully encapsulated in nanofibers with a bead-like and increased diameter compared to the morphology of nanofibers without thymol. The pre-complex of CM-HAS-thymol provided a high encapsulation efficiency (EE, 96.59%) for thymol in PUL nanofibers. The inclusion complexation also provided enhancement of thermal stability for thymol and PUL nanofibers. Therefore, the designed nanofibers with thermal stability, degradability, and antibacterial properties have the capacity to be used in food applications.

Amoxicillin-loaded multilayer pullulan-based nanofibers maintain long-term antibacterial properties with tunable release profile for topical skin delivery applications

Unique physiochemical and biological properties of nanofibers along with the choice of a wide variety of materials for both fabrication and tunable release patterns make nanofibers an ideal option for drug delivery. Loading antibacterial agents into nanofibers has attracted great deal of attention. Whilst there are several studies focusing on applying new generations of antibacterial materials, antibiotics are still the gold standard in clinical applications. Therefore, we aimed at introducing antibiotic-loaded nanofiber substrates with potential for topical skin delivery applications, reduced consumption of antibiotics and increased storage time. We applied Amoxicillin (AMX) as a model drug with low solubility and detected the presence of AMX in our nanofibers using FTIR and Raman spectroscopy. AMX-loaded Pullulan (Pull) nanofibers proved to maintain the antibacterial properties of the AMX drug after electrospinning, and to preserve the antibacterial properties for at least 8 months storage. The release trend can be tuned from burst release in mono-layer AMX:Pull nanofibers to sustained release if sandwiching the Pull layer between two hydrophobic electrospun layers (e.g. PLGA biopolymer). The AMX-loaded Pull construct can be considered as a novel nanofibrous solid dispersion of a poorly water-soluble drug for efficient topical application of antibiotics in wound healing and skin treatments.

Carbon Nanodots-Embedded Pullulan Nanofibers for Sulfathiazole Removal from Wastewater Streams.

Carbon nanodots (CNDs)-embedded pullulan (PUL) nanofibers were developed and successfully applied for sulfathiazole (STZ) removal from wastewater streams for the first time. The CNDs were incorporated into PUL at 0.0%, 1.0%, 2.0%, and 3.0% (w/w) to produce M1, M2, M3, and M4 nanofibers (PUL-NFs), respectively. The produced PUL-NFs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), thermal gravimetric analysis (TGA) and Differential scanning calorimetry (DSC) and applied for STZ removal from aqueous solutions through pH, kinetics, and equilibrium batch sorption trials. A pH range of 4.0–6.0 was observed to be optimal for maximum STZ removal. Pseudo-second order, intraparticle diffusion, and Elovich models were suitably fitted to kinetics adsorption data (R2 = 0.82–0.99), whereas Dubinin–Radushkevich, Freundlich, and Langmuir isotherms were fitted to equilibrium adsorption data (R2 = 0.88–0.99). STZ adsorption capacity of PUL-NFs improved as the amount of embedded CNDs increased. Maximum STZ adsorption capacities of the synthesized PUL-NFs were in the order of: M4 > M3 > M2 > M1 (133.68, 124.27, 93.09, and 35.04 mg g−1, respectively). Lewis acid–base reaction and π-π electron donor–acceptor interactions were the key STZ removal mechanisms under an acidic environment, whereas H-bonding and diffusion were key under a basic environment. Therefore, CNDs-embedded PUL-NFs could be employed as an environmentally friendly, efficient, and non-toxic adsorbent to remove STZ from wastewater streams.

Multi-layer PLGA-pullulan-PLGA electrospun nanofibers for probiotic delivery

Encapsulation of bacteria into a polymer matrix can potentially enhance the delivery of probiotics. In this study, we present a multilayer electrospun construct to facilitate enhanced delivery of probiotics, where the internal layer is loaded with bacterial cells and the external layers sandwich the internal layer for increased protection and potential mucoadhesion properties. In this proof of concept study, Lactobacillus rhamnosus GG (LGG), which is a robust and well-studied probiotic strain, was encapsulated into pullulan nanofibers, with two electrospun PLGA (Poly-lactic-co-glycolic acid) layers covering it. According to our in vitro study, there was a large decrease in the viability of LGG released from the monolayer sample (LGG:pullulan). However, the multilayer construct maintained excellent viability and an acceptable storage potential. Our in vivo competition study showed that LGG, delivered by multilayer construct, were able to survive intestinal transit and were recovered from all segments of the intestine. Not only did the multilayer construct perform as well as non-encapsulated spray-dried cells in terms of viability and establishment of LGG, but it also showed some cases of increased LGG colonization from fibers in jejunum and cecum compared to spray-dried LGG three days after dosage. We believe, the multilayer construct has the potential to enhance the delivery of the strains that require additional protection, and can benefit from mucus embedment with the help of the covering layers. Particularly, the use of electrospun fibers for the protection of next-generation probiotics sensitive to oxygen and/or gastric conditions could have major commercial interest.

Encapsulation of Drug‐Loaded Graphene Oxide‐Based Nanocarrier into Electrospun Pullulan Nanofibers for Potential Local Chemotherapy of Breast Cancer

AbstractWith the high rate of mortality associated with breast cancer among women, breast cancer treatment has attracted great deal of attention globally. To reduce the exposure of body organs to high cytotoxicity of the common chemotherapeutic drugs, local co‐delivery of selected chemotherapeutics has emerged as a solution. In this work, an electrospun composite including a co‐drug‐loaded graphene oxide‐based nanocarrier is fabricated for local anticancer applications. To increase dispersion and cellular uptake of graphene oxide (GO), first, the hydroxyl groups at the edges of GO are grafted by poly (epichlorohydrin) (PCH) to form GO‐PCH. Then, the hydroxyl end groups of PCH are grafted (g) with hyperbranched polyglycerol (HPG) leading to formation of oxygen‐rich nanocarrier (GO‐PCH‐g‐HPG). Paclitaxel (PTX) and curcumin (Cur) are loaded into the GO‐PCH‐g‐HPG nanocarrier, and are encapsulated into pullulan nanofibers using electrospinning. SEM, FTIR, and UV–Vis characterizations confirmed the presence and distribution of the nanocarrier inside the nanofibers. The drugs showed a sustained release (93 h) from the nanocarrier‐loaded nanofibers in the neutral pH (7.4). According to MTT assay and optical microscopy, a synergistic cytotoxicity effect of PTX and Cur is observed. The nanocarrier‐encapsulated nanofiber system represents a novel tunable drug delivery system for local chemotherapeutic applications.

Fabrication and characterization of rizatriptan loaded pullulan nanofibers as oral fast-dissolving drug system

Fast drug-dissolving systems have been introduced to mediate the drugs which are difficult to swallow or having poor water solubility. Rizatriptan benzoate is a drug recommended for the patients of migraine which effect one out of every 5 women and 15 men globally. But least bioavailability (40%–50%) and reduced on set action always increases the demand of a drug carrier in order to overcome these limitations. Here in pullulan mediated fast drug-dissolving systems was developed by using rizatriptan benzoate as a model drug. While rizatriptan loaded pullulan nanofiber mat was prepared via electrospinning. Physiochemical outcomes (SEM, FTIR, and XRD) revealed good compatibility of pullulan nanofibers and rizatriptan thoroughly distributed on electrospun NFs matrix. Wetting time (1 s) and dissolutions time (3 s) suggests burst release of the drug from the polymers matrix as dissolution time is directly proportional with release profile. Further, this was confirmed by UV-release profile studies and maximum release was found within 30 s. In vitro release kinetics were analyzed by fitting the results with higuchi and korsmeyer models.

Fast Dissolving Electrospun Nanofibers Fabricated from Jelly Fig Polysaccharide/Pullulan for Drug Delivery Applications.

The fast-dissolving drug delivery systems (FDDDSs) are developed as nanofibers using food-grade water-soluble hydrophilic biopolymers that can disintegrate fast in the oral cavity and deliver drugs. Jelly fig polysaccharide (JFP) and pullulan were blended to prepare fast-dissolving nanofiber by electrospinning. The continuous and uniform nanofibers were produced from the solution of 1% (w/w) JFP, 12% (w/w) pullulan, and 1 wt% Triton X-305. The SEM images confirmed that the prepared nanofibers exhibited uniform morphology with an average diameter of 144 ± 19 nm. The inclusion of JFP in pullulan was confirmed by TGA and FTIR studies. XRD analysis revealed that the increased crystallinity of JFP/pullulan nanofiber was observed due to the formation of intermolecular hydrogen bonds. The tensile strength and water vapor permeability of the JFP/pullulan nanofiber membrane were also enhanced considerably compared to pullulan nanofiber. The JFP/pullulan nanofibers loaded with hydrophobic model drugs like ampicillin and dexamethasone were rapidly dissolved in water within 60 s and release the encapsulants dispersive into the surrounding. The antibacterial activity, fast disintegration properties of the JFP/pullulan nanofiber were also confirmed by the zone of inhibition and UV spectrum studies. Hence, JFP/pullulan nanofibers could be a promising carrier to encapsulate hydrophobic drugs for fast-dissolving/disintegrating delivery applications.