The study assessed constraints faced by agriculture researchers in conducting research uptake activities in tertiary institutions in Rivers State, Nigeria. Simple random sampling was used to select one hundred and eighty-two agriculture researchers from the three universities that have faculty of Agriculture in Rivers State. Data for the study was collected using a questionnaire and in-depth interviews while analysis of data was carried out using, mean, percentages and the Binary Logit regression model. Results showed that 98% of the researchers were involved in research uptake activities. Publication of journal articles (76%), convening training workshops (60%) and organizing seminars (59%) were the major research uptake activities conducted by agriculture researchers in the State. Insufficient funds (x=3.68), lack of basic facilities (x=3.54) and poor synergy between researchers and policymakers (x =3.48) were major constraints faced by agriculture researchers in conducting research uptake activities. Agriculture researchers’ socioeconomic characteristics were not significant determinants of agriculture researchers’ level of involvement in research uptake activities in the study area. The provision of basic structural facilities and financial support by university management will aid agricultural researchers’ involvement in research uptake activities.