Background:The burden of foodborne illnessisconsidered to behigh across the world.Basedonthe Knowledge-Attitude-Practice(KAP) model, physician's awareness is essentialforconductingindividualized treatments, thus reducingtheburdenof foodborne illness. However, there have been no validated questionnaires specific to the awareness of physicians with foodborne diseases.This study aims to develop and validateaKAPquestionnaire for physiciansto assess their awarenessaboutthediagnosis and managementof foodborneillness. MethodsThe questionnaire was developed in three phases: a comprehensive literature review, face and content validity, followed by a reliability test by internal consistency. A cross-sectional study was designed in Abha,Saudi Arabia. Physicians (n=125) wereopportunisticallyrecruited from both public and private primary healthcarecenters.The questionnaire'scontent and validity were confirmed by experts in their corresponding fields. After signing the informed consent, the study participants received the questionnaire to evaluate theirKAPson foodborne diseases. Results:A totalof160 physicians from both public and private primary health carecenterswere approached to enrol125study participantsintothe survey(response rate78.13%). Of the 31itemsdesigned for assessing theKAPof physicians on foodborne illnesses, threeitems were excluded afterCronbach'sα analysis. In total, 29 items wereincluded inthe final set of the questionnaire.Results of different validity and reliability analyses suggestthe questionnaire hasahigh faceandcontent validity as well as good reliability in internal consistencyand stability. Conclusions:This study introduces a newly developed questionnaire with good reliability and validity values that can assess physician's awarenessof foodborne disease.The awareness questionnaire,as a study instrument,hadafavourable acceptanceamong physicians. It is a sound method for evaluating and measuring levels of foodborne disease-related awareness among physicians in Abha, Saudi Arabia.