The workload on health workers in Public health centers can be seen from aspects such as tasks that arecarried out based on their main functions. The purpose of this study was to analyze the factors that influencethe workload of health workers in the Namrole Public health center and the WamsisiPublic health center inSouth Buru Regency. This type of research is a quantitative cross-sectional study design. The populationin this study were all health workers with the status of civil servants who worked in the NamrolePublichealth center as many as 73 officers and WamsisiPublic health center as many as 27 officers. The samplingtechnique in this study was proportional random sampling to obtain a sample of the NamrolePublic healthcenter as many as 58 officers and WamsisiPublic health center as many as 22 officers. The results showedthat there was no influence of the level of education on the workload of health workers in the Namrolepublichealth center and the Wamsisipublic health center in South Buru regency (p = 0.622> 0.05). Working timesignificantly affected the workload (p = 0.000 <0.05) and years of service are protective factors for theworkload of employees at the Namrolepublic health center and the Wamsisipublic health center in SouthBuru regency (p = 0.053> 0.05). To the Regional Government of South Buru Regency to emphasize moreregulations related to working hours, especially for employees at public health care centers, so that they areable and comfortable to carry out their duties and functions effectively and efficiently with optimal worktime and not excessive.