Pubescens Extract Research Articles

PENGARUH EKSTRAK BUAH Carica pubescens Lenne & K. Koch YANG TUMBUH DI BEBERAPA TEMPAT DI INDONESIA TERHADAP PENYEMBUHAN LUKA MUKOSA RONGGA MULUT

<em>The aim of this study is to examine the effect of Carica pubescens</em> Lenne & K. Koch <em>extract to the epihtelialization on the wound healing of gingival mucosa and to examine the effect of C. pubescens extract from different region (Dieng and Cangar) to the epithelialization process.Twenty eight adult male albino rats were devided into four group, we created cutlet along 1 cm on the mandibular gingival mucosa below insisiv teeth. K1 group were treated with aquadest while K2 group were treated with C. pubescens extract from Dieng, K3 group were treated with C. pubescens extract from Cangar, and K4 group were treated with policresulen preparations. At the end of fifth day, the rats were sacrificed and the mandibular gingival mucosa were collected for histologist preparations. The result of this study shows that K3 group has the thickest gingival epithel layers while there is no significant different among four group for the thikness gingival epithel layers</em>

Simultaneous determination of seven coumarins by UPLC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Huo Luo Xiao Ling Dan or single-herb extract

A simple, sensitive and reliable ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for simultaneous quantitation of seven coumarins, the bio-active ingredients of Huo Luo Xiao Ling Dan (HLXLD), in rat plasma. The liquid-liquid extraction method with ether-dichloromethane (2:1, v/v) was used to prepare the plasma samples. Analytes and internal standard (IS) of bifendate were separated on a Shim-pack XR-ODS column (75mm×3.0mm, 2.2μm particles) using gradient elution with the mobile phase consisting of methanol and 0.05% formic acid in water at a flow rate of 0.4mL/min. Detection was performed on a triple quadrupole (TQ) tandem mass spectrometry equipped with an electrospray ionization source in the positive ionization and multiple reaction monitoring (MRM) mode. The lower limits of quantitation (LLOQ) were 0.03-0.25ng/mL for all the analytes. Intra- and inter-day precision and accuracy of the seven analytes were well within acceptance criteria (15%). The matrix effect and the mean extraction recoveries of the analytes and IS from rat plasma were all within satisfaction. The validated method has been successfully applied to compare pharmacokinetic profiles of the seven active ingredients in rat plasma between normal and arthritic rats after oral administration of HLXLD, Angelica pubescens extract and Notopterygium incisum extract, respectively. Results showed that there were remarkable differences in pharmacokinetic properties of the analytes among the different groups.

Vasodilation and radical-scavenging activity of imperatorin and selected coumarinic and flavonoid compounds from genus Casimiroa

Hypertension is a very widespread condition which is not strictly considered as an illness but if not countered, progressively causes damage to all tissues and loss in their functionality. For this reason the find of new antihypertensive agents is prominent and medicinal plants and their derivatives are valuable for the purpose. The genus Casimiroa (Rutaceae) includes plants from Central America and Mexico; among these, Casimiroa edulis Llave et Lex. and Casimiroa pubescens Ramirez are the most relevant species, even for their medicinal uses. The decoction of leaves and seeds is traditionally taken as a tea mainly to lower blood pressure. The object of this research was the study of vascular activity of coumarinic and flavonoid compounds isolated from seeds of Casimiroa spp. in comparison with Casimiroa edulis and Casimiroa pubescens extracts. The phenolic compounds isolated from Casimiroa were herniarin (Her), imperatorin (Imp), 8-geranyloxypsoralen (GOP) and 5,6,2′,3′,4′-pentamethoxyflavone (PMF). All these compounds induced vasorelaxation on rat arterial tissues although with different effectiveness. To study the cellular mechanisms of the vasorelaxation exhibited by imperatorin, we used selective inhibitors of different receptors and enzymes, such as atropine, pyrilamine, nifedipine, L-NAME and DETC. In a further step of this research, we evaluated the radical-scavenging activity of Casimiroa extracts and isolated compounds by means of DPPH assay. In general, we observed that the scavenging activities increased in a concentration-dependent manner for all substances. The phenolic compounds highlight a synergism of vasodilation and antioxidant activity which may be very useful in the management of cardiovascular diseases. Among the evaluated compounds, imperatorin shows a significant vasorelaxant activity even higher than acetylcholine and similar to nitrite, and also useful antiradical capabilities. All these properties suggest its possible role against hypertension and vasculopathies, even if in vivo studies are needed to determine the actual applications.

Anti-Oxidative and Anti-Obesity Activities of Tetrapanax papyriferus and Siegesbeckia pubescens Extracts and their Synergistic Anti-Obesity Effects

In this study, the anti-oxidative and anti-obesity activities of two medicinal herb extracts, Tetrapanax papyriferus (TP) and Siegesbeckia pubescens (SP), were evaluated using DPPH radical scavenging activity assay, lipase enzyme inhibition assay, and the cell culture model system. Both methanol extracts of TP and SP showed DPPH radical scavenging activities dose-dependently, and the of DPPH radical scavenging activities of the two medicinal herbs were 65.23 and 47.79 , respectively. Furthermore, both extracts suppressed effectively lipase enzyme activity dose-dependently. Moreover, TP and SP extracts significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride (TG) contents on 3T3-L1 preadipocytes in a dose-dependent manner without cytotoxicity. Their anti-obesity effect was modulated by cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins (), and peroxisome proliferator-activated receptor () gene and protein expressions. Furthermore, TP and SP possessed a synergistic effect on anti-obesity activity. The identification of the active compounds that confer the anti-obesity activity of TP and SP might be needed.

Simultaneous Determination of 4 Flavonoids in Extracts of Phyllostachys pubescens Leaves by HPLC

In this study,4 flavonoids in Phyllostachys pubescens extracts i.e.orientin,vitexin,vitexin-4'-O- glucoside,and vitexin-2'-O-rhamnose glycoside were simultaneously detected.The HPLC detected conditions were as follows:Kromasil-C18 column(5μm,4.6 mm×250 mm,Akzol Nobel,Sweden),mobile phase was tetrahydrofuran:acetonitrile:methanol:0.5%aqueous acetic acid(10.3:1:0.7:88),isocratic elution,the detection wavelength was 360 nm,column temperature was 25℃,and 1.0 mL·min~(-1) flow velocity.The average recoveries of orientin,vitexin,vitexin-4'-O-glucoside,and vitexin-2'-O-rhamnose glycoside were 98.5%, 98.1%,99.1%,and 98.7%,respectively.The relative standard deviations were 0.8%,0.7%,0.8%and 0.6%(n = 5 ),respectively.This HPLC method has been proved to be a method with high efficiency,rapid analysis,high sensitivity and good reproducibility for detection of flavonoids in P.pubescens extracts.

Age-dependent vasorelaxation of Casimiroa edulis and Casimiroa pubescens extracts in rat caudal artery in vitro

Leaf and seed decoctions of Casimiroa spp. are used in Mexican traditional medicine to treat high blood pressure. Previous researches showed as Casimiroa extracts are able to induce relaxation of rat aortic and caudal arteries. To study the influence of the aging, we determined the vascular effect induced by extracts of Casimiroa edulis and Casimiroa pubescens in arterial tissues from young and old rats. The activity of Casimiroa edulis extracts: hexanic-leaf (Ce5), methanolic-leaf (Ce6), hexanic-seed (Ce7) and methanolic-seed (Ce8), and Casimiroa pubescens: hexanic-leaf (Cp9), methanolic-leaf (Cp10), hexanic-seed (Cp11) and methanolic-seed (Cp12) were investigated in precontracted caudal arteries of young (4 months) and old (20 months) rats. The Casimiroa extracts tested at 20 μg/ml induced vasorelaxation in phenylephrine-precontracted arterial tissues both in young and old arterial tissues. Methanolic seed extracts of Casimiroa edulis (Ce8) and Casimiroa pubescens (Cp12) caused a higher relaxation in young than in old arterial tissues. Nifedipine (0.01 μM) did not change the vasorelaxation induced by Casimiroa edulis extract either in young and old rat arterial tissues. The vasorelaxation induced by Casimiroa edulis and Casimiroa pubescens extracts is decreased from aging since the effects were higher in young than in old rat arterial tissues. However, the methanolic-seed extracts of both plant species induced a relevant vasorelaxation also in old arterial tissues. Thus the results support the traditional use of Casimiroa decoctions as antihypertensive, also in elderly.

Terpenic subfraction of Pterodon pubescens induces apoptosis of K562 leukemic cells by modulating gene expression

Deregulation of cell proliferation and apoptosis is linked to malignant cell development. Leukemia is the most frequent cancer in children, and plants are important sources for new potential anti-cancer agents. Although anti-tumoral effects have been shown for Pterodon pubescens extracts, the mechanisms are still obscure. This study describes in Pterodon pubescens a furane diterpene only reported in Pterodon polygalaeflorus, the methyl-6α-acetoxy-7β-hydroxyvouacapan-17β-oate, indicated by HRMS and 13C-NMR analysis, and demonstrates some mechanisms of the anti-leukemia action of its terpene subfraction SF5. SF5 induced cytotoxic and anti-proliferative effects on K562 cells. Increased sub-G1 nuclei and Annexin V+-FITC cells confirmed apoptosis of leukemic cells by treatment of these cells with SF5. Down-regulation of DNMT1 gene transcription and over-expression of Apaf-1 mRNA suggested that SF5 may be inducing apoptosis of K562 cells by epigenetic up-regulation of pro-apoptotic proteins involved in the mitochondrial intrinsic pathway.

Applications of HPLC-MS in compound Ilex pubescens extract study

In this paper, high performance liquid chromatography (HPLC) along with mass spectrometry (MS) and HPLC along with a diode array detector (DAD) was used to study the compound Ilex pubescens extract. Two ionization techniques: electro spray ionization (ESI) and atmospheric pressure chemical ionization (APCI) were used in this work. The liquid chromatograms obtained by DAD, total ion chromatograms (TIC) from positive-and negative-ion ESI-MS and the positive-and negative-ion APCI-MS were compared. The liquid chromatograms obtained by TIC from ESI-MS provided more information on chromatographic peaks than those obtained by DAD or TIC from APCI-MS. It is suggested that the fingerprints of the compound Ilex pubescens extract should be provided by the liquid chromatograms obtained by DAD together with TIC from the negative-ion ESI-MS. The molecular weights of the nine main components in an HPLC-DAD chromatogram were determined by the corresponding positive-and negative-ion ESI and the positive-and negative-ion APCI mass spectra information. In the liquid chromatogram obtained by TIC from the negative-ion ESI-MS, the molecular weights of 23 main components were determined based on the corresponding positive-and negative-ion ESI mass spectra information.

Toxicity of the ethanol extract of Magonia pubescens on larvae Aedes aegypti

Crude ethanol extracted from Magonia pubescens trunks was utilized to prove its effect in the intestinal tract of Aedes aegypti 3rd instar larvae. Exposure times (2, 4, 6, 8, 10, 12, and 13 hours) were tested to verify when the morphological alterations begin to occur, in the intestinal tract of the larvae. The toxic effect of the extract was mainly in the midgut, beginning at the anterior midgut and followed through to posterior midgut. The main alterations observed were partial or total cell destruction, high citoplasmatic vacuolization, increase of subperitrophic space, cell hypertrophy and the epithelium did not maintain its monolayer appearance. The alterations began after four hours of exposure to M. pubescens extract. Observations of tissue sections from larvae treated for different lengths of time revealed a wide variation in the degree of damage between exposure periods and midgut larvae region. The present study provides evidence regarding the mode of action of the M. pubescens extract and suggests its potential utilization as a larvicide to control Aedes aegypti mosquito.