Pseudorca Crassidens Research Articles

A citizen science approach to long‐term monitoring of humpback whales (Megaptera novaeangliae) off Sydney, Australia

AbstractThe Cape Solander Whale Migration Study is a citizen science project that annually counts northward migrating humpback whales (Megaptera novaeangliae) off Cape Solander, Sydney, Australia. Dedicated observers have compiled a 20‐year data set (1997–2017) of shore‐based observations from Cape Solander's high vantage point. Using this long‐term data set collected by citizen scientists, we sought to estimate the humpback whale population trend as it continues to recover postexploitation. We estimated an exponential growth rate of 0.099 (95% CI = 0.079–0.119) using a generalized linear model, based on observer effort (number of observation days) and number of whales observed, equating to 10% per annum growth in sightings since 1997. We found that favorable weather conditions for spotting whales off Cape Solander consisted of winds <30 km/hr from a southerly through a north westerly direction. Incidental observations of other cetacean species included the endangered blue whale (Balaenoptera musculus) and data deficient species such as killer whales (Orcinus orca) and false killer whales (Pseudorca crassidens). Citizen science‐based studies can provide a cost‐effective approach to monitoring wildlife over the time necessary to detect change in a population. Information obtained from citizen science projects like this may help inform policy makers responsible for State and Federal protection of cetaceans in Australian waters and beyond.

Asian elephants exhibit post-reproductive lifespans

BackgroundThe existence of extended post-reproductive lifespan is an evolutionary puzzle, and its taxonomic prevalence is debated. One way of measuring post-reproductive life is with post-reproductive representation, the proportion of adult years lived by females after cessation of reproduction. Analyses of post-reproductive representation in mammals have claimed that only humans and some toothed whale species exhibit extended post-reproductive life, but there are suggestions of a post-reproductive stage for false killer whales and Asian elephants. Here, we investigate the presence of post-reproductive lifespan in Asian elephants using an extended demographic dataset collected from semi-captive timber elephants in Myanmar. Furthermore, we investigate the sensitivity of post-reproductive representation values to availability of long-term data over 50 years.ResultsWe find support for the presence of an extended post-reproductive stage in Asian elephants, and that post-reproductive representation and its underlying demographic rates depend on the length of study period in a long-lived animal.ConclusionsThe extended post-reproductive lifespan is unlikely due to physiological reproductive cessation, and may instead be driven by mating preferences or condition-dependent fertility. Our results also show that it is crucial to revisit such population measures in long-lived species as more data is collected, and if the typical lifespan of the species exceeds the initial study period.

Whistle Classification of Sympatric False Killer Whale Populations in Hawaiian Waters Yields Low Accuracy Rates

Cetaceans are ecologically important marine predators, and designating individuals to distinct populations can be challenging. Passive acoustic monitoring provides an approach to classify cetaceans to populations using their vocalizations. In the Hawaiian Archipelago, three genetically distinct, sympatric false killer whale (Pseudorca crassidens) populations coexist: a broadly distributed pelagic population and two island-associated populations, an endangered main Hawaiian Islands (MHI) population and a Northwestern Hawaiian Islands (NWHI) population. The mechanisms that sustain the genetic separation between these overlapping populations are unknown but previous studies suggest that the acoustic diversity between populations may correspond to genetic differences. Here, we investigated whether false killer whale whistles could be correctly classified to population based on their characteristics to serve as a method of identifying populations when genetic or photographic-identification data are unavailable. Acoustic data were collected during line-transect surveys using towed hydrophone arrays. We measured 50 time and frequency parameters from whistles in 16 false killer whale encounters identified to population and used those measures to train and test random forest classification models. Random Forest models that included three populations correctly classified 42% of individual whistles overall and resulted in a low kappa coefficient, κ = 0.15, indicating low agreement between models and the true population. Whistles from the MHI population showed the highest correct classification rate (52%) compared to pelagic and NWHI whistles (42% and 36%, respectively). Pairwise random forest models classifying pelagic and MHI whistles proved slightly more accurate (62% accuracy, κ = 0.24), though a similar pelagic-NWHI model did not (56% accuracy, κ = 0.12). Results suggest that the time-frequency whistle characteristics are not suitable to confidently classify encounters to a specific false killer whale population, although certain features of whistles produced by the endangered MHI population allow for overall higher classification accuracy. Inclusion of other vocalization types, such as echolocation clicks, and alternative whistle variables may improve correct classification success for these sympatric populations.

First record of false killer whales (Pseudorca crassidens) in the Falkland Islands (Malvinas)

The false killer whale (Pseudorca crassidens) is primarily distributed across tropical, subtropical and warm temperate waters, with few records originating above 50° latitude in each hemisphere. In February 2013, a mass stranding of 22 false killer whales occurred on the east coast of the Falkland Islands (Malvinas; 51.83°S, 58.24°W). Of the 20 sexed animals, 11 were male and 9 were female. Total body lengths ranged from 396 to 581 cm, and the stranded animals likely consisted entirely of mature adults. There were no significant differences between the sexes in proportional body measurements, with the exception of proportional dorsal fin height which was significantly higher in males. Tooth counts ranged from 14 to 18 in the upper jaws, and from 14 to 20 in the lower jaws. Four stomachs were investigated, of which three were empty and one contained a single Onychoteuthidae squid (Onykia ingens) beak. Mitochondrial control region sequences from the stranded animals indicated greater genetic connectivity between the south Pacific and south Atlantic than between the south and north Atlantic ocean basins. Given the absence of full necropsies, the underlying reasons for the stranding could not be ascertained. This mass stranding represents the first confirmed record of false killer whales in the Falkland Islands, and provides new information on the species in the southernmost part of its distributional range.

Stranded false killer whales, Pseudorca crassidens, in Southern South America reveal potentially dangerous silver concentrations

Silver (Ag) is a non-essential metal known to bioaccumulate in aquatic organisms. We determined Ag concentrations in five false killer whales stranded in South America. Silver concentrations (in dry weight basis) range as 6.62–10.78 μg g−1 in liver, 0.008–7.41 μg g−1 in spleen, 0.004–5.71 μg g−1 in testis, 0.757–1.69 μg g−1 in kidney, 0.011–0.078 μg g−1 in lung and < 0.01–0.038 μg g−1 in muscle, whereas in the single samples of uterus and ovary were 0.051 and 0.023 μg g−1; respectively. Overall, Ag concentration in liver and kidney exceeded the cetacean toxic thresholds, proposed as “unhealthy concentrations” and “critically dangerous” in liver and kidney. These results warrant further eco-toxicological studies, to examine biological effects of elevated silver levels for individuals and to assess the species' conservation status with respect to marine pollution.

How to tell them apart? Discriminating tropical blackfish species using fin and body measurements from photographs taken at sea

AbstractThe misidentification of species and populations is a hindrance to effective cetacean management. We devised a method of species identification using 10 fin and body measurements obtainable from at‐sea photographs, and demonstrated its ability to distinguish four species of Hawaiian “blackfish”: pygmy killer, melon‐headed, short‐finned pilot, and false killer whales. Measurements from photos of 382 known individuals were converted into 14 ratios and reduced using nonmetric multidimensional scaling and principal component analysis. The first three components of the PCA explained 81% of variance among species. Ratios indicated by PCA and NMDS were tested using analysis of variance, and results identified three fin ratios that had distinct means across all four species: height/base, depth (from anterior insertion) at trailing edge apex/depth at topmost point, and width (from leading edge) at posterior point/base. Dual analysis of adults and all age classes showed near‐identical sources of variance, 90% similarity in interspecies ratio relationships, and overlapping ratio means and ranges. Results suggest similar ontogenetic growth across these four species, and confirm the efficacy of this discrimination technique for all age classes. This study established a reliable means of distinguishing these cetacean species, which will improve the efficacy of management in areas with sympatric distributions.

From banana fields to the deep blue: Assessment of chlordecone contamination of oceanic cetaceans in the eastern Caribbean

In the French West Indies (Caribbean), the insecticide Chlordecone (CLD) has been extensively used to reduce banana weevil (Cosmopolites sordidus) infestations in banana plantations. Previous studies have shown high CLD concentrations in freshwater and coastal communities of the region. CLD concentrations, however, have not yet been assessed in marine top predators. We investigated CLD concentrations in cetacean blubber tissues from Guadeloupe, including Physeter macrocephalus, Lagenodelphis hosei, Stenella attenuata and Pseudorca crassidens. Chlordecone was detected in all blubber samples analysed, with the exception of four P. macrocephalus. Concentrations (range: 1 to 329 ng·g−1 of lipid weight) were, however, lower than those found in species from fresh and brackish water. Ecological factors (open ocean habitat), CLD kinetics, and cetacean metabolism (high or specific enzymatic activity) might explain low concentrations found in cetacean blubber. Future analyses that include internal organ sampling would help to confirm CLD levels observed in this study.

New Distribution Records of Cetaceans from the Federated States of Micronesia

Photographs of a whale initially found alive in mangroves in Pohnpei, Federated States of Micronesia (FSM), in July 2016 document the first record of the genus Kogia in the Caroline Islands. With its small size and what can be seen of its dentition, it most closely resembles a dwarf sperm whale, Kogia sima, but the possibility of it being an immature pygmy sperm whale, K. breviceps, cannot be excluded. In addition, we report the first documented record of a short-finned pilot whale, Globicephala macrorhynchus for Pohnpei State, and the first confirmed records of killer whales, Orcinus orca, and false killer whales, Pseudorca crassidens, for the FSM and the Caroline Islands, respectively.

Abundance estimates for management of endangered false killer whales in the main Hawaiian Islands

Abundance estimates for management of endangered false killer whales in the main Hawaiian Islands

First record of common bottlenose dolphin (Tursiops truncatus) in Canadian Pacific waters

BackgroundCommon bottlenose dolphins (Tursiops truncatus) are distributed globally in tropical and warm-temperate waters with coastal and offshore ecotypes known. In the eastern North Pacific Ocean, common bottlenose dolphins are typically found in offshore waters as far as 41° N and in coastal waters as far as 38° N. Despite considerable survey effort, the species has not been previously recorded in Canadian Pacific waters.ResultsOn 29 July 2017, a group of approximately 200 common bottlenose dolphins were observed together with approximately 70 false killer whales (Pseudorca crassidens) in waters of 16.5° C at 50° N during a pelagic seabird and marine mammal survey off the west coast of northern Vancouver Island, British Columbia, Canada.ConclusionsThis sighting represents the only occurrence of common bottlenose dolphins recorded in Canadian Pacific waters and, to our knowledge, is the most northerly record for this species in the eastern North Pacific. It is also the first sighting record of false killer whales in non-coastal waters in British Columbia, Canada. The occurrence of both species may be associated with a prolonged period of warming in offshore regions of the eastern North Pacific.

Four odontocete species change hearing levels when warned of impending loud sound.

Hearing sensitivity change was investigated when a warning sound preceded a loud sound in the false killer whale (Pseudorca crassidens), the bottlenose dolphin (Tursiops truncatus), the beluga whale (Delphinaperus leucas) and the harbor porpoise (Phocoena phocoena). Hearing sensitivity was measured using pip-train test stimuli and auditory evoked potential recording. When the test/warning stimuli preceded a loud sound, hearing thresholds before the loud sound increased relative to the baseline by 13 to 17 dB. Experiments with multiple frequencies of exposure and shift provided evidence of different amounts of hearing change depending on frequency, indicating that the hearing sensation level changes were not likely due to a simple stapedial reflex.

High status of mercury and selenium in false killer whales (Pseudorca crassidens, Owen 1846) stranded on Southern South America: A possible toxicological concern?

The study was carried out to determine Hg and Se concentrations in false killer whales stranded on the Estrecho de Magallanes, Chile, South America. Tissue samples of five mature specimens were analyzed (two females and three males). Mean Hg concentration in liver 1068 (234) μg g−1 dry weight (DW) (standard deviation in parenthesis) was markedly higher than those in kidney 272 (152) μg g−1 DW, lung 423 (325) μg g−1 DW, spleen 725 (696) μg g−1 DW, muscle 118 (94) μg g−1 DW and testicle 18.0 (2.8) μg g−1 DW. Mean Se concentration in liver, 398 (75) μg g−1 DW, was higher than those in kidney 162 (69) μg g−1 DW, lung 128 (84) μg g−1 DW, spleen 268 (245) μg g−1 DW, muscle 47 (38) μg g−1 DW and testicle 25.4 (2.1) μg g−1 DW. Positive correlations were found between Hg and Se molar concentrations in muscle, lung, spleen and kidney. Molar ratio of Se/Hg in liver, lung and muscle were <1, but those in kidney and testicle were markedly >1 suggesting a Se protection against Hg toxicity. In all the examined specimens Hg values exceeded the toxic thresholds defined for hepatic damage in marine mammals, with Se/Hg molar ratios below 1 implying limited protective action of Se. Generally, our results showed that individuals are carrying a significant burden, reflecting a high exposure to this toxic metal. This constitutes the first report on Hg and Se levels for a large subantarctic odontocete in South America region, providing insights into their contamination status and with information to the understanding of possible impacts on wild populations.

First report of the occurrence and whole-genome characterization of Edwardsiella tarda in the false killer whale (Pseudorca crassidens).

Although several Edwardsiella tarda infections have been reported, its pathogenic role in marine mammals has not been investigated at the genome level. We investigated the genome of E. tarda strain KC-Pc-HB1, isolated from the false killer whale (Pseudorca crassidens) found bycaught in South Korea. The obtained genome was similar to that of human pathogenic E. tarda strains, but distinct from other Edwardsiella species. Although type III and VI secretion systems, which are essential for the virulence of other Edwardsiella species, were absent, several virulence-related genes involved in the pathogenesis of E. tarda were found in the genome. These results provide important insights into the E. tarda infecting marine mammals and give valuable information on potential virulence factors in this pathogen.

Long-term monitoring of circulating progesterone and its relationship to peripheral white blood cells in female false killer whales Pseudorca crassidens.

Long-term monitoring of circulating progesterone levels in three captive female false killer whales, Pseudorca crassidens, was conducted to characterize their reproductive events and to reveal the relationship between their estrous cycles or pregnancies and peripheral white blood cell (WBC) counts. Blood samples were collected at 2–3-day intervals or on a weekly-to-monthly basis for up to 10 years, from 2006 to 2017. In two mature females (initial body lengths of 4.22 and 4.07 m), some cyclic progesterone elevations were detected during the study period; the estimated mean (± SE) estrous cycle length was 40.5 ± 0.7 days (n=12). The seasonality of ovulation, estimated from the elevation of progesterone levels, varied among individuals or years, and ovulation did not occur every year. The third female (3.26 m) showed progesterone elevations, despite irregular cycles after sexual maturity, and became pregnant. The progesterone levels during pregnancy ranged from 7.3 to 42.2 ng/ml, and the gestation period lasted for 14 months until parturition. The mean WBC counts during estrous cycles were the lowest before the progesterone levels began to increase and then gradually increased toward the luteal phase. The WBC counts were significantly higher during pregnancy than before and were particularly high in early pregnancy. To the best of our knowledge, this is the first report of the relationship between the estrous cycle or pregnancy and WBC counts in cetaceans.

Disguised Bionic Sonar Signal Waveform Design with Its Possible Camouflage Application Strategy for Underwater Sensor Platforms

The covertness of an active sonar is a very important issue and the sonar signal waveform design problem is studied to improve covertness of the system. Many marine mammals produce call pulses for communication and echolocation, and existing interception systems normally classify these biological signals as ocean noise and filter them out. Based on this, a disguised sonar signal waveform design approach with its camouflage application strategy for underwater sensor platforms is proposed by utilizing bio-inspired steganography. We first construct bionic sonar signal waveforms which are very close to the true whale whistle, and then embed these constructed bionic sonar signal waveforms into the true whale call trains to hide the real sonar signal waveforms. According to the time-frequency structure of the true whale whistle, a bionic sonar signal model is established to generate the proposed sonar signal waveforms. A single sonar signal is used to measure the range of the target and a combination of two sonar signals is utilized for measuring its speed. A high-performance range and speed measurement algorithm is deduced in detail. Based on the constructed signal waveforms and the characteristics of false killer whale call trains, a camouflage application strategy is designed to improve the camouflage ability of the sonar signal sequence. Finally, simulation results are provided to verify the performance of the proposed method.

The incidence of bent dorsal fins in free-ranging cetaceans.

Laterally bent dorsal fins are rarely observed in free-ranging populations of cetaceans, contrary to captivity, where most killer whale Orcinus orca adult males have laterally collapsed fins. This topic has been poorly explored, and data/information on its occurrence and possible causes are limited. The present study: (i) undertakes a review of the available information on bent dorsal fins in free-ranging cetaceans, and updates it with new records, (ii) reports on the proportion of bent fins in different study populations, and (iii) discusses possible causes. An empirical approach based on bibliographic research and compilation of 52 new records collected worldwide resulted in a total of 17 species of cetaceans displaying bent dorsal fins. The species with the highest number of records (64%) and from most locations was O.orca. On average, individuals with bent dorsal fins represent <1% of their populations, with the exception of false killer whales Pseudorca crassidens and O.orca. While line injuries associated with fisheries interactions may be the main cause for P.crassidens, and the vulnerability to health issues caused by the evolutionary enlargement of the fin may be the cause for O.orca adult males, factors contributing to this abnormality for other species are still unclear. The occurrence of bent dorsals could be influenced by a set of variables rather than by a single factor but, irrespective of the cause, it is suggested that it does not directly affect the animals' survivorship. While still rare in nature, this incident is more common (at least 101 known cases) and widespread (geographically and in species diversity) than hypothesized, and is not confined only to animals in captive environments. Investigation into the occurrence of bent fins may be an interesting avenue of research.

Relationship between head size and biosonar transmit and receive beams in odontocetes

The unique shape of a dolphin head, the different specie specific shape and the internal head structure suggest a very complex propagation mechanism for the biosonar signals to travel from the phonic lips into the water. Despite these factors, a circular planar aperture of the appropriate diameter can produce a transmission beam that resembles the corresponding beam of a dolphin. In the similar manner, the reception process is also very complex and not completely understood. Once again, despite the complexity involving the reception of biosonar echoes, a simple circular planar aperture of the right diameter can have a receiving beam that approximate or resemble the receiving beam of odontocetes. For both transmission and reception of broadband biosonar signals, the size of the head compared to the wavelength of the signal will determine the degree of directionality of the beams in odontocetes in a similar manner as a planar transducer. Beam pattern data from Tursiops truncates, Delphinapterus leucas, Pseudorca crassidens, and Phocoena phocoena will be used to demonstrate the relationship between head size and directionality for the transmit signal. Receiving beam pattern data from Tursiops truncates and Phocoena phocoena will be used in a similar manner as for the transmit beam.

Review of Underwater and In-Air Sounds Emitted by Australian and Antarctic Marine Mammals

The study of marine soundscapes is a growing field of research. Recording hardware is becoming more accessible; there are a number of off-the-shelf autonomous recorders that can be deployed for months at a time; software analysis tools exist as shareware; raw or preprocessed recordings are freely and publicly available. However, what is missing are catalogues of commonly recorded sounds. Sounds related to geophysical events (e.g. earthquakes) and weather (e.g. wind and precipitation), to human activities (e.g. ships) and to marine animals (e.g. crustaceans, fish and marine mammals) commonly occur. Marine mammals are distributed throughout Australia’s oceans and significantly contribute to the underwater soundscape. However, due to a lack of concurrent visual and passive acoustic observations, it is often not known which species produces which sounds. To aid in the analysis of Australian and Antarctic marine soundscape recordings, a literature review of the sounds made by marine mammals was undertaken. Frequency, duration and source level measurements are summarised and tabulated. In addition to the literature review, new marine mammal data are presented and include recordings from Australia of Omura’s whales (Balaenoptera omurai), dwarf sperm whales (Kogia sima), common dolphins (Delphinus delphis), short-finned pilot whales (Globicephala macrorhynchus), long-finned pilot whales (G. melas), Fraser’s dolphins (Lagenodelphis hosei), false killer whales (Pseudorca crassidens), striped dolphins (Stenella coeruleoalba) and spinner dolphins (S. longirostris), as well as the whistles and burst-pulse sounds of Australian pygmy killer whales (Feresa attenuata). To date, this is the most comprehensive acoustic summary for marine mammal species in Australian waters.

Stranding survey as a framework to investigate rare cetacean records of the north and north-eastern Brazilian coasts.

Marine mammal stranding events are used as an important tool for understanding cetacean biology worldwide. Nonetheless, there are vast gaps of knowledge to be filled in for a wide range of species. Reputable information is required regarding species from large baleen whales to sperm and beaked whales, as well as pelagic dolphins. This paper describes new cetacean records from north and north-eastern Brazil, which are both the least surveyed areas regarding aquatic mammals. Regular beach surveys were conducted to recover cetacean carcasses along the coast of Pará beginning November 2005. At the coasts of the Maranhão and Piauí states, the surveys were conducted between 2003 and 2013. From 2003 to 2014, 34 strandings of cetaceans were registered. The study provides four additional species records’ in the area based on strandings (Balaenoptera borealis, Balaenoptera physalus, Peponocephala electra, and Pseudorca crassidens). A mass stranding of Guiana dolphins (Sotalia guianensis, N = 12), the most common species for the region, was reported for the first time. The records presented herein are of special concern, since they expand the knowledge on cetaceans from the Brazilian coast. In addition, this study conducted an analysis to verify the similarity between cetacean compositions described for north and north-eastern Brazil and the southern Caribbean region. The results showed a high similarity between these regions, proving the connection with the Caribbean cetacean fauna.

Evidence for a postreproductive phase in female false killer whales Pseudorca crassidens

BackgroundA substantial period of life after reproduction ends, known as postreproductive lifespan (PRLS), is at odds with classical life history theory and its causes and mechanisms have puzzled evolutionary biologists for decades. Prolonged PRLS has been confirmed in only two non-human mammals, both odontocete cetaceans in the family Delphinidae. We investigate the evidence for PRLS in a third species, the false killer whale, Pseudorca crassidens, using a quantitative measure of PRLS and morphological evidence from reproductive tissues.ResultsWe examined specimens from false killer whales from combined strandings (South Africa, 1981) and harvest (Japan 1979-80) and found morphological evidence of changes in the activity of the ovaries in relation to age. Ovulation had ceased in 50% of whales over 45 years, and all whales over 55 years old had ovaries classified as postreproductive. We also calculated a measure of PRLS, known as postreproductive representation (PrR) as an indication of the effect of inter-population demographic variability. PrR for the combined sample was 0.14, whereas the mean of the simulated distribution for PrR under the null hypothesis of no PRLS was 0.02. The 99th percentile of the simulated distribution was 0.08 and no simulated value exceeded 0.13. These results suggest that PrR was convincingly different from the measures simulated under the null hypothesis.ConclusionsWe found morphological and statistical evidence for PRLS in South African and Japanese pods of false killer whales, suggesting that this species is the third non-human mammal in which this phenomenon has been demonstrated in wild populations. Nonetheless, our estimate for PrR in false killer whales (0.14) is lower than the single values available for the short-finned pilot whale (0.28) and the killer whale (0.22) and is more similar to working Asian elephants (0.13).