Pseudomonas Monteilii Research Articles

Plant growth-promotion and biofortification of chickpea and pigeonpea through inoculation of biocontrol potential bacteria, isolated from organic soils.

Seven strains of bacteria [Pseudomonas plecoglossicida SRI-156, Brevibacterium antiquum SRI-158, Bacillus altitudinis SRI-178, Enterobacter ludwigii SRI-211, E. ludwigii SRI-229, Acinetobacter tandoii SRI-305 and Pseudomonas monteilii SRI-360; demonstrated previously for control of charcoal rot disease in sorghum and plant growth-promotion (PGP) in rice] were evaluated for their PGP and biofortification traits in chickpea and pigeonpea under field conditions. When treated on seed, the seven selected bacteria significantly enhanced the shoot height and root length of both chickpea and pigeonpea over the un-inoculated control. Under field conditions, in both chickpea and pigeonpea, the plots inoculated with test bacteria enhanced the nodule number, nodule weight, root and shoot weights, pod number, pod weight, leaf weight, leaf area and grain yield over the un-inoculated control plots. Among the seven bacteria, SRI-229 was found to significantly and consistently enhance all the studied PGP and yield traits including nodule number (24 and 36%), nodule weight (11 and 44%), shoot weight (22 and 20%), root weight (23 and 16%) and grain yield (19 and 26%) for both chickpea and pigeonpea, respectively. When the harvested grains were evaluated for their mineral contents, iron (up to 18 and 12%), zinc (up to 23 and 5%), copper (up to 19 and 8%), manganese (up to 2 and 39%) and calcium (up to 22 and 11%) contents in chickpea and pigeonpea, respectively, were found enhanced in test bacteria inoculated plots over the un-inoculated control plots. This study further confirms that the selected bacterial isolates not only have the potential for PGP in cereals and legumes but also have the potential for biofortification of mineral nutrients.

A novel biosensor for p-nitrophenol based on an aerobic anode microbial fuel cell

P-nitrophenol is one of the most common contaminants in chemical industrial wastewater, and in situ real-time monitoring of PNP cannot be achieved by conventional analytical techniques. Here, a two-chamber microbial fuel cell with an aerobic anode chamber was tested as a biosensor for in situ real-time monitoring of PNP. Pseudomonas monteilii LZU-3, which was used as the biological recognition element, can form a biofilm on the anode electrode using PNP as a sole substrate. The optimal operation parameters of the biosensor were as follows: external resistance 1000Ω, pH 7.8, temperature 30°C, and maximum PNP concentration 50mgL−1. Under these conditions, the maximum voltages showed a linear relationship with PNP concentrations ranging from 15±5 to 44±4.5mgL−1. Furthermore, we developed a novel portable device for in situ real-time monitoring of PNP. When the device was applied to measure PNP in wastewater containing various additional aromatic compounds and metal ions, the performance of the biosensor was not affected and the correlation between the maximum voltages and the PNP concentrations ranging from 9±4mgL−1 to 36 ± 5mgL−1 was conserved. The results demonstrated that the MFC biosensor provides a rapid and cost-efficient analytical method for real-time monitoring of toxic and recalcitrant pollutants in environmental samples.

Bio-mediated oxidative resolution of racemic 2-substituted 1,2,3,4-tetrahydroquinolines

Whole cell of Pseudomonas monteilii ZMU-T01 strains mediated oxidative resolution of racemic 2-substituted 1,2,3,4-tetrahydroquinolines has been successfully described. A series of highly enantioselective 2-substituted 1,2,3,4-tetrahydroquinoline derivatives were obtained in up to 50% conversion and >99% ee.

Bioreduction of the C[dbnd]C double bond with Pseudomonas monteilii ZMU-T17: one approach to 3-monosubstituted oxindoles

An efficient whole cell-mediated bioreduction of 3-methylene-2-oxindoles has been developed, affording a range of 3-monosubstituted oxindoles in moderate to good yields (41–82%) with Pseudomonas monteilii ZMU-T17 as biocatalyst. Additionally, a possible reaction pathway for this bioreduction of CC double bond was proposed.

Bioinoculants and Vermicompost Improve Ocimum basilicum Yield and Soil Health in a Sustainable Production System

With respect to the adverse effects of chemical fertilizers on the environment and their higher cost, an alternative method of improving soil fertility has been tested. Application of vermicompost (VC) (produced from de‐oiled waste) along with efficient bioinoculants (nitrogen fixer: Bacillus sp., JN700924; phosphate solubilizer: Bacillus megaterium, ATCC 14581; plant growth promoter: Pseudomonas monteilii, HQ 995498; arbuscular mycorrhizal fungus (AMF): Glomus intraradices) in different combinations were evaluated on Ocimum basilicum. VC along with bioinoculants‐treated plots showed a significant improvement in biomass (15.1–56%), essential oil content (26.7–48.2%), and essential oil yield (44.7–108.4%) over control. The major constituent of essential oil (i.e., methyl chavicol) was significantly improved in T3 (VC + Bacillus sp.), T5 (VC + P. monteilii), and T6 (VC + G. intraradices) treated plants as compared to control (T1). Significant improvement in microbial counts, percent root colonization of AMF, and alkaline phosphatase activity were recorded; maximum in bioinoculants along with VC‐treated plots. Improvement in soil organic carbon (4.2–51.16%), NH4+‐N (27.0–55.3%), NO3–‐N (−8 to 60.33%], available P (15.2–43.5%), and available K (0.4–20.8%) were found in VC along with bioinoculants‐treated plots. The diethylene triamine pentaacetic acid extractable Fe (109–154 mg kg−1 soil) was more influenced by VC along with bioinoculants than other micronutrients (Cu, Mn, and Zn). The results suggest that an integrated use of VC with bioinoculants improves soil health and essential oil yield of O. basilicum.

Selective ciprofloxacin antibiotic detection by fluorescent siderophore pyoverdin

Fluorescent siderophore pyoverdin (PVD) was produced from a soil isolate Pseudomonas monteilii strain MKP 213. The PVD was purified near to homogeneity and applied for the fluorescent chemosensing of various antibiotics in aqueous solution (pH=7.0). Upon addition of ciprofloxacin, PVD showed new UV–vis absorption bands at 252 and 321nm due to an internal charge transfer mechanism. Also, the addition of ciprofloxacin induced a highly selective fluorescence enhancement of PVD with a 13nm blue shift from 458 to 445nm. The combination of a long peptide chain along with the chromophore unit of PVD generates a converging cleft for ciprofloxacin recognition with LOD and LOQ of 7.13μM and 21.6μM, respectively without interference from other studied antibiotics. The association constant (Ka) of PVD with ciprofloxacin was calculated to be as low as 1.40×105M−1 using Benesi-Hildebrand plot depicting its significance in detection. The pharmaceutical tablet analysis measures the sensing with negligible matrix effect and quantitative recovery.

Unusual microorganisms and antimicrobial resistances in a group of Syrian migrants: Sentinel surveillance data from an asylum seekers centre in Italy.

Three years of civil war in Syria have caused death and increase of communicable diseases. The suffering population has been forced to migrate creating a fertile condition for epidemic spread of infection within the refugee camps. Forty-eight Syrian migrants, upon their arrival in Italy, were accommodated at the asylum seekers centre of Castelnuovo di Porto. They received a physical examination and were subjected to microbiological surveillance by blood, rectal, pharyngeal and nasal swabs collection and delivering to the Clinical Pathology and Microbiology Laboratory of the University Campus Bio-Medico of Rome. All refugees resulted negative for HBV, HCV and HIV infections. In swabs a large number of unusual gram-negative bacteria species were isolated, such as Pseudomonas putida, Pseudomonas monteilii, Pseudomonas fulva, Pseudomonas moselii, Aeromonas veronii, Aeromonas caviae, Aeromonas hydrophila, Acinteobacter guilloviae, Acinteobacter lowffii; Acinetobacter johnsonii; Acinteobacter tjernbergae; Pantoea agglomerans; Pantoea calida. Among isolates, strains resistant to carbapenems, ESBL producers and methicillin resistant were found. The microbiological surveillance performed represents a useful action to understand refugees health status and to trace unusual microorganisms movement even carriers of antimicrobial resistance during migrants traveling.

The Metagenome of Utricularia gibba's Traps: Into the Microbial Input to a Carnivorous Plant.

The genome and transcriptome sequences of the aquatic, rootless, and carnivorous plant Utricularia gibba L. (Lentibulariaceae), were recently determined. Traps are necessary for U. gibba because they help the plant to survive in nutrient-deprived environments. The U. gibba's traps (Ugt) are specialized structures that have been proposed to selectively filter microbial inhabitants. To determine whether the traps indeed have a microbiome that differs, in composition or abundance, from the microbiome in the surrounding environment, we used whole-genome shotgun (WGS) metagenomics to describe both the taxonomic and functional diversity of the Ugt microbiome. We collected U. gibba plants from their natural habitat and directly sequenced the metagenome of the Ugt microbiome and its surrounding water. The total predicted number of species in the Ugt was more than 1,100. Using pan-genome fragment recruitment analysis, we were able to identify to the species level of some key Ugt players, such as Pseudomonas monteilii. Functional analysis of the Ugt metagenome suggests that the trap microbiome plays an important role in nutrient scavenging and assimilation while complementing the hydrolytic functions of the plant.

Bio-inoculants and vermicompost influence on yield, quality of Andrographis paniculata, and soil properties

Andrographis paniculata is a source of diterpenoids and 2′-oxygenated flavonoids, which are of utility in pharmaceutical industry and ayurvedic formulations. With the aim of producing quality herb, an experiment was conducted with different combinations of bio-inoculants and vermicompost (VC) in controlled condition. It was observed that the highest Leaf:Stem (L:S) ratio, fresh herb yield and andrographolide yield (0.82, 300g pot−1 and 29.8gkg−1 dry herb, respectively) was recorded when the soil was incorporated with VC along with Azotobacter chrococcum (T3). Further there was a significant improvement in all soil fertility parameters. However, when all the bio-inoculants (A. chrococcum+Bacillus megaterium+Pseudomonas monteilii+Glomus intraradices) were mixed with VC, there was a significant improvement in soil dehydrogenase, alkaline, and acidic phosphatase activity. A positive correlation coefficient (p<0.01) could be derived amongst plant growth, yield and soil properties (r=0.45–0.85). The study suggests that application of the bio-inoculants and organic fertilizers can enhance productivity while maintaining the desired quality of the herb.

Isolation of VIM-2-producing Pseudomonas monteilii clinical strains disseminated in a tertiary hospital in northern Spain.

We describe here the occurrence of blaVIM-2 in 10 carbapenem-resistant Pseudomonas monteilii strains isolated from different clinical samples from patients at the University Hospital Marqués de Valdecilla in northern Spain. All the blaVIM-2-harboring P. monteilii isolates possessed a class 1 integron, with the cassette array [intI1_blaVIM-2_aac(6')-Ib_qacEΔ1_sul1]. Our results show the emergence of VIM-2-producing multidrug-resistant species other than Pseudomonas aeruginosa or Pseudomonas putida in a Spanish hospital. P. monteilii, although sporadically isolated, should also be considered an important metallo-β-lactamase (MBL) reservoir.

Impact of plant growth promoting Pseudomonas monteilii PsF84 and Pseudomonas plecoglossicida PsF610 on metal uptake and production of secondary metabolite (monoterpenes) by rose-scented geranium (Pelargonium graveolens cv. bourbon) grown on tannery sludge amended soil

Bacterial strains PsF84 and PsF610 were isolated from tannery sludge polluted soil, Jajmau, Kanpur, India. 16S rRNA gene sequence and phylogenetic analysis confirmed the taxonomic affiliation of PsF84 as Pseudomonas monteilii and PsF610 as Pseudomonas plecoglossicida. A greenhouse study was carried out with rose-scented geranium (Pelargonium graveolenscv. bourbon) grown in soil treated with tannery sludge in different proportions viz. soil: sludge ratio of 100:0, 25:75, 50:50, 75:25 and 0:100 to evaluate the effects of bacterial inoculation on the heavy metal uptake. The isolates solubilized inorganic phosphorus and were capable of producing indole acetic acid (IAA) and siderophore. The isolate PsF84 increased the dry biomass of shoot by 44%, root by 48%, essential oil yield 43% and chlorophyll by 31% respectively over uninoculated control. The corresponding increase with the isolate PsF610 were 38%, 40%, 39% and 28%, respectively. Scanning electron microscopic (SEM) studies reveal that the Cr(VI) accumulation resulted in breakdown of vascular bundles and sequesters Cr(VI) in roots. The glandular trichomes (GT) were investigated using SEM studies as these glands are probably the main site of essential oil synthesis. Owing to its wide action spectrum, these isolates could serve as an effective metal sequestering and bioinoculants due to the production of IAA, siderophore and solubilization of phosphate for geranium in metal-stressed soil. The present study has provided a new insight into the phytoremediation of metal-contaminated soil.

Molecular identification and characterization of Pseudomonas sp. NCCP-407 for phenol degradation isolated from industrial waste

Phenol is a toxic pollutant found in effluent of numerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collected from industrial waste and enriched in mineral salt medium (MSM) contained 300 mg/L phenol. The strain was identified based on 16S rRNA gene analysis as Pseudomonas species and the phylogenetic analysis affiliated the strain with Pseudomonas monteilii (AF064458) as the most closely related species. Phenol tolerance of the strain in MSM supplemented with various concentrations of phenol indicates that the strain NCCP-407 can grow best at 750 mg L−1 phenol. The strain showed complete degradation of 750 mg L−1 phenol in 56 hours when supplement as a sole source of carbon and energy with the average degradation rate of 28 mg L−1h−1. The doubling time was recorded approximately as 12.49 h−1. The present study suggests that this strain is efficient in phenol degradation and can be used in treatment of wastewater containing phenol.

Stereoselective oxidation of sulfides to optically active sulfoxides with resting cells of Pseudomonas monteilii CCTCC M2013683

• A whole cell biocatalyst was successfully applied for directly oxidation of sulfides. • Pseudomonas monteilii CCTCC M2013683 exhibited high activity and substrate tolerance. • No by-products were formed in this bioxidation process. In this paper, the ability of Pseudomonas monteilii to selectively oxidize aryl sulfides has been investigated. Especially, a toluene-degrading strain, P. monteilii (CCTCC M2013683), was isolated form soil samples that exhibit excellent activity, stereoselectivity and high substrate tolerance to perform oxidation of organic sulfides. Several biotransformation parameters such as the employment of substrate concentration, cell density, reaction temperature, pH value and cosolvent system resulted crucial in the optimization of the processes. Under optimal reaction parameters, whole-cells catalyzed the asymmetric oxidation of sulfides to chiral sulfoxides ( R )- 2a – 2e with 54–99% yields in 63–99% enantiomeric excess ( ee ), respectively.

Draft genome of formaldehyde-degrading strain, Pseudomonas monteilii IOFA19

The draft genome of Pseudomonas monteilii strain IOFA19 isolated from deep-sea sediment of the Indian Ocean was sequenced to gain insights into its efficient metabolic pathway for degrading formaldehyde. The annotated genome of this strain was subjected to comparative analysis with the genomes of existing sequenced P. monteilii strains QM, SB3101, and SB3078. Differences in the overall genomic content were observed, and relative genes that were hypothetically involved in the formaldehyde-degrading mechanism were identified.

Effects of co-inoculation of native Rhizobium and Pseudomonas strains on growth parameters and yield of two contrasting Phaseolus vulgaris L. genotypes under Cuban soil conditions

Plant growth-promoting rhizobacteria (PGPR) have been isolated from legume nodules, displaying a potential to enhance nodulation, growth and yield of legume plants when co-inoculated with Rhizobium. This study genetically characterizes bacteria isolated from bean root nodules in Cuba and investigates the effect of Rhizobium–Pseudomonas co-inoculation on common bean (Phaseolus vulgaris L.) genotypes under two different Cuban soil conditions. Bacteria were identified by partial sequencing of the 16S rDNA gene. Growth-promoting effects of Rhizobium pisi–Pseudomonas monteilii co-inoculation were evaluated under field conditions in consecutive years. Two contrasting bean genotypes (BAT-477 and DOR-364) were co-inoculated and grown in two different soil types (Cambisol and Oxisol) in Cuban farm areas. Several growth parameters were evaluated at three time points during the crop cycle (21, 42 and 87 days after planting). In total, 20 strains were isolated belonging to six different genera, i.e. Arthrobacter, Chryseobacterium, Enterobacter, Stenotrophomonas, Pseudomonas and Rhizobium. It was observed that native Rhizobium–Pseudomonas co-inoculation as compared to single Rhizobium inoculation increased the nodulation, growth parameters and yield of the different genotypes. The response of BAT-477 was more pronounced than that of DOR-364. These findings contribute to the understanding of the interplay between Rhizobium, PGPR and the plant host under different soil conditions. Importantly, co-inoculation with R. pisi and P. monteilii could be an effective biofertilization strategy for common bean production.

Degradation of Norgestrel by Bacteria from Activated Sludge: Comparison to Progesterone

Natural and synthetic progestagens in the environment have become a concern due to their adverse effects on aquatic organisms. Laboratory studies were performed to investigate aerobic biodegradation of norgestrel by bacteria from activated sludge in comparison with progesterone, and to identify their degradation products and biotransformation pathways. The degradation of norgestrel followed first order reaction kinetics (T1/2 = 12.5 d), while progesterone followed zero order reaction kinetics (T1/2 = 4.3 h). Four and eight degradation products were identified for norgestrel and progesterone, respectively. Six norgestrel-degrading bacterial strains (Enterobacter ludwigii, Aeromonas hydrophila subsp. dhakensis, Pseudomonas monteilii, Comamonas testosteroni, Exiguobacterium acetylicum, and Chryseobacterium indologenes) and one progesterone-degrading bacterial strain (Comamonas testosteroni) were successfully isolated from the enrichment culture inoculated with aerobic activated sludge. To our best knowledge, this is the first report on the biodegradation products and degrading bacteria for norgestrel under aerobic conditions.

Vermicompost from biodegraded distillation waste improves soil properties and essential oil yield of Pogostemon cablin (patchouli) Benth

Pre-treatment of patchouli lignocellullosic distillation waste with a consortium of microbes viz. Trichoderma harzianum – ATCC PTA-3701, Pseudomonas monteilii – HQ995498, Bacillus megaterium – ATCC 14581 and Azotobacter chroococcum – MTCC 446 significantly enhanced the bio-degradation of cellulose, hemicelluloses and lignin by 58.44%, 29.44% and 65.23%, respectively and improved the yield of vermicompost by 15%. Application of bioinoculant-enriched vermicompost (BEVC) in patchouli reduced the percent disease index (PDI) of Rhizoctonia root-rot by 36.36% and improved the essential oil yields by 59.74%, 37.70% and 37.96 as compared to plots receiving untreated (not enriched) vermicompost (VC), bioinoculant-enriched compost (BEC) and chemical fertilizers (CF), respectively. Furthermore, BEVC application resulted in a marked improvement in physical (bulk density and water holding capacity) and chemical properties (pH, percent organic carbon, available N, P and K) of the soil. In conclusion, BEVC from distillation waste can be safely utilized as a bio-organic input unambiguously particularly in situations where the use of chemical fertilizers and pesticides is restricted like organic agriculture.

Indigenous PAH-degrading bacteria from oil-polluted sediments in Caleta Cordova, Patagonia Argentina

Indigenous bacteria with the capability to degrade polycyclic aromatic hydrocarbons (PAH) were isolated from polluted sediment samples recovered from Caleta Cordova by using selective enrichment cultures supplemented with phenanthrene. Bacterial communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in order to detect changes along enrichment culture and relationships with the representative strains subsequently isolated. Members of these communities included marine bacteria such as Lutibacter, Polaribacter, Arcobacter and Olleya, whose degradation pathway of PAH has not been studied yet. However, isolated bacteria obtained from this enrichment comprised the genus Pseudomonas, Marinobacter, Salinibacterium and Brevibacterium. The ability of isolates to grow and degrade naphthalene, phenanthrene and pyrene was demonstrated by detection of the residual substrate by HPLC. Archetypical naphthalene and catechol dioxygenase genes were found in two isolates belonging to genus Pseudomonas (Pseudomonas monteilii P26 and Pseudomonas xanthomarina N12), suggesting biodegradation potential in these sediments. The successful bacterial isolation with the ability to degrade PAH in pure culture suggest the possibility to study and further consider strategies like growth stimulation in situ, in order to increase the intrinsic bioremediation opportunities in the polluted Caleta Cordova harbor.

Technology for improving essential oil yield of Ocimum basilicum L. (sweet basil) by application of bioinoculant colonized seeds under organic field conditions

Two year field studies indicated that seed treatment of Ocimum basilicum var. CIM-Saumya with efficient bioinoculants (Pseudomonas monteilii – strain CRC1, Cronobacter dublinensis – strain CRC3 and Bacillus spp. – strain AZHGF1) can significantly improve the essential oil yield (45–56%); maximum essential oil yield was observed in plants inoculated with P. monteilii (56%) followed by C. dublinensis (49%) and Bacillus spp. (45%). The content of essential oil was also significantly improved (15%) when inoculated with P. monteilii compared to un-inoculated control. The higher concentrations of linalool (40.40%) and β-caryophyllene (14.15%) were observed in the plants inoculated with P. monteilii. P. monteilii also produced maximum biomass yield; an increase of about 55% followed by C. dublinensis (42%) and Bacillus spp. (30%). To the best of our knowledge this might be an exclusive report suggesting the use of bioinoculants for higher yields and disease management for organic growers of sweet basil.

Development of a bioreactor for remediation of textile effluent and dye mixture: A plant–bacterial synergistic strategy

The objective of the present work was to develop a plant–bacterial synergistic system for efficient treatment of the textile effluents. Decolorization of the dye Scarlet RR and a dye mixture was studied under in vitro conditions using Glandularia pulchella (Sweet) Tronc., Pseudomonas monteilii ANK and their consortium. Four reactors viz. soil, bacteria, plant and consortium were developed that were subjected for treatment of textile effluents and dye mixture. Under in vitro conditions G. pulchella and P. monteilii showed decolorization of the dye Scarlet RR (SRR) by 97 and 84%, within 72 and 96 h respectively, while their consortium showed 100% decolorization of the dye within 48 h. In case of dye mixture G. pulchella, P. monteilii and consortium-PG showed an ADMI removal of 78, 67 and 92% respectively within 96 h. During decolorization of SRR G. pulchella showed induction in the activities of enzymes lignin peroxidase and DCIP reductase while P. monteilii showed induction of laccase, DCIP reductase and tyrosinase, indicating their involvement in the dye metabolism. High Performance Liquid Chromatography (HPLC), Fourier Transform Infra Red Spectroscopy (FTIR) and High Performance Thin Layer Chromatography (HPTLC) confirmed the biotransformation of SRR and dye mixture into different metabolites. Soil, bacteria, plant and consortium reactors performed an ADMI removal of 42, 46, 62 and 93% in the first decolorization cycle while it showed an average ADMI removal of 21, 27, 59 and 93% in the next three (second, third and fourth) decolorization cycles respectively for the dye mixture within 24 h. Consortium reactor showed an average ADMI removal of 95% within 48 and 60 h for textile effluents A and B respectively for three decolorization cycles, while it showed an average TOC, COD and BOD removal of 74, 70 and 70%, 66, 72 and 67%, and 70, 70 and 66% for three decolorization cycles of the dye mixture (second, third and fourth decolorization cycles), effluent A and effluent B respectively. Degradation of the textile effluents and dye mixture into different metabolites by the consortium reactor was confirmed using HPLC and FTIR. Phytotoxicity studies revealed the non-toxic nature of the metabolites of degradation of dye mixture, effluents A and B by consortium reactor. The developed consortial reactor system performed efficient treatment of the dye mixture and textile effluents, and can be used for treating large amounts of textile effluents when implemented as a constructed wetland by proper engineering approach.