Prussian Blue Nanocubes Research Articles

Biomimetic Transparent Slippery Surface for the Locomotion of Photocontrol Droplets and Bubbles.

Directed transportation and collection of liquids and bubbles play a vital role in the survival of ecosystems. Among them, the optical response control is widely used in the fields of microfluidic chips and chemical synthesis because of its high remote operation and fast response speed. However, due to poor light transmission, the development direction of traditional near-infrared (NIR) absorbing materials in the field of visualization is limited, and there are few reports of manufacturing an operating platform that can realize the directional movement of droplets/bubbles on a single platform. Here, a transparent photo-responsive PBFS platform is prepared for droplet and bubble manipulation by coating the etched glass substrate with Prussian blue (PB) nanocubes. When near-infrared (NIR) irradiation on the PBFS platform, PB nanocubes trigger heat production by photothermal means, due to the action of Marangoni force, the surface tension on the left and right sides of the droplets and bubbles is not uniform, forming a surface tension gradient, thereby driving the movement of the droplets and bubbles. The control platform has good application potential in the field of microchemical reaction and biomedical engineering and brings new solutions to the field of transparent photothermal materials.

In-situ growth of Ni, Co-Prussian blue nanocubes on molten salt etched lamellar Ti3C2Tx/Ni for enhanced hybrid capacitive deionization

The design and development of high-performance electrode materials are crucial for improving the desalination efficiency of hybrid capacitive deionization (HCDI). In this study, a Ti3C2Tx/Ni/NiCo-PBA composite is synthesized by in-situ growing NiCo Prussian blue (PB) nanocubes on Ti3C2Tx/Ni sheets, serving as an electrode for HCDI. The Ti3C2Tx sheets provide excellent conductivity and a suitable substrate for the growth of NiCo-PBA nanocubes. The Ni nanoparticles help prevent the oxidation of Ti3C2Tx sheets, and provide a source of Ni for NiCo-PBA synthesis. Additionally, the NiCo-PBA nanocubes effectively inhibit re-stacking of Ti3C2Tx sheets, enlarge crystal lattice spacing, and facilitate diffusion and storage of salt ions. Consequently, the AC||Ti3C2Tx/Ni/NiCo-PBA HCDI device exhibits a high salt removal capacity of 36.62 mg g−1, low energy consumption of 11.14 Wh m−3, and water recovery of 61.86 %. Importantly, the AC||Ti3C2Tx/Ni/NiCo-PBA device achieves a good salt removal capacity of 53.22 mg g−1 in a high concentration NaCl solution (2000 μS cm−1). After 20 cycles, the desalination capacity remains stable, and the structure of Ti3C2Tx/Ni/NiCo-PBA is intact, indicating excellent cycling stability. Additionally, the desalination mechanism of Ti3C2Tx/Ni/NiCo-PBA electrode is thoroughly investigated.

A new strategy for constructing ZIF-67@PBA core-shell 3D cross-heterostructures for improving fire safety of TPU at ultra-low addition amount

Thermoplastic polyurethane (TPU) has an extensive application in many different industries. However, serious fire hazards and smoke toxicity have been the main reason limiting its wide application. Therefore, it is necessary and urgent to perform flame retardant and smoke suppression treatment for TPU. In recent years, metal-organic framework compounds (MOFs) have very promising application prospects in the fields of flame-retardant polymer composites. However, there is a problem of low flame-retardant efficiency for the original MOFs alone in polymer composites. It is reported the multi-level and multi-structured flame-retardant system has better flame-retardant efficiency than the traditional structures. So, the dual MOF core-shell heterostructure may have more effective heat reduction and smoke suppression than any single component. In this paper, a core-shell 3D cross-heterostructures nanohybrid (ZIF-67H@PBA) was prepared using ZIF-67H as the host MOF and Prussian blue nanocubes (PBA) as the guest MOF. It has been found that TPU/ZIF-67H@PBA composites with ultra-low additions have excellent fire safety. Compared with those of pure TPU, the peak heat release rate (PHRR), total smoke release (TSP), and smoke factor (SF) of the samples with 0.5wt% ZIF-67H@PBA were reduced by 33.6 %, 47 %, and 61 %, respectively. At the same time, a cone calorimeter (CCT), a homemade soot sampling device and a gas chromatography-mass spectrometry (GC–MS) coupling with each other were constructed and used to demonstrate the most realistic effects of flame retardants in terms of smoke suppression and toxicity reduction. This work provides a new strategy to design TPU flame retardants.

Synergistic effect of Prussian Blue, Ceria, and reduced graphene oxide in ternary nanocomposite PBNCs-CeO2-rGO for electrochemical detection of hydrogen peroxide

Graphene-based ternary composites incorporating rare earth metals have garnered significant attention for their potential in low-detection applications of hydrogen peroxide (H2O2), owing to their cost-effectiveness and facile synthesis methods. In this study, we have reported the synergistic effect of Prussian blue nanocubes (PBNCs), reduced graphene oxide (rGO), and ceria nanoparticles (CeO2) in the ternary nanocomposite, i.e., PBNCs-CeO2-rGO for a low detection of H2O2. The enhanced electrocatalytic activity of ternary composite as compared to bare PBNCs and CeO2 nanoparticles was evidenced in different electrochemical methods such as Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and Amperometry. The synthesized material was comprehensively characterized using X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), Fourier-transform infrared spectroscopy (FTIR), and Raman spectroscopy. Remarkably, the synthesized material exhibited a low detection limit of up to 2.08 nM and a high sensitivity of 8671.6 μA mM−1 cm−2. Furthermore, the synthesized material demonstrated excellent selectivity, good reproducibility, and high stability, highlighting its potential for various sensing applications.

A Multimodal Antibacterial Platform for Visualizing Reactive Oxygen Species Generation and Promoting Wound Healing

AbstractPhoto‐activated therapeutic strategies have great potential for preventing bacterial infections, especially infections associated with drug‐resistant bacteria, owing to their minimal tendency to induce drug resistance. A single course of light‐based therapeutics may lack the desired efficacy. The use of a high‐power excitation source or high doses of photo‐activated agents may cause excessive damage to normal tissues. Herein, a multimodal antibacterial platform that combines photodynamic and photothermal therapies has been developed. The fluoro‐substituted coumarin compound (TCF) and Prussian blue nanocubes (PB NCs) are integrated into the bacterial cellulose (BC) to form a versatile wound dressing (BC‐TCF‐PB). Fluoro‐substitution in TCF is found to enhance its production of 1O2 by boosting the intersystem crossing. Furthermore, the generated 1O2 can react with the thiocarbonyl group in TCF to form a highly emissive product. In situ self‐reporting fluorescence of TCF allows for the visualization of 1O2 generation, and assessment of the bacterial eradication. In addition, the BC‐TCF‐PB platform exhibits synergistic antibacterial effects on the methicillin‐resistant Staphylococcus aureus (MRSA), and provides an effective solution for infection control and wound healing. Thus, the BC‐TCF‐PB dressing, with self‐reporting of the reactive biocide generation in situ, has offered valuable insights into effective wound management.

Salivary Amylase-Responsive Buccal Tablets Wipe Out Chemotherapy-Rooted Refractory Oral Mucositis.

Oral mucositis (OM) is the most common and refractory complication of cancer chemotherapy and radiotherapy, severely affecting patients' life quality, lowering treatment tolerance, and discouraging patient compliance. Current OM delivery systems mostly affect the comfort of patient use and lead to poor compliance and unsatisfactory effects. Herein, salivary amylases (SAs)-responsive buccal tablets consisting of porous manganese-substituted Prussian blue (PMPB) nanocubes (NCs), anti-inflammatory apremilast (Apr) and starch controller have been engineered. PMPB NCs with large surface area can serve as carriers to load Apr, and their multienzyme-mimicking activity enables them to scavenge reactive oxygen species (ROS), which thus synergize with Apr to mitigate inflammation. More significantly, the starch controller can respond to abundant SAs in the oral cavity and realize the cascade, continuous, and complete drug release after enzymatic decomposition, which not only aids with high tissue affinity to prolong the resistance time but also improves the comfort of use. The preclinical study reveals that contributed by the above actions, such buccal tablets mitigate inflammation, promote endothelium proliferation and migration, and accelerate wound healing for repressing chemotherapy-originated intractable OM with positive oral microenvironment and shorter recovery time, thus holding high potentials in clinical translation.

Prussian blue nanocubes with peroxidase-like activity for polyphenol detection in commercial beverages.

The present study describes an efficient method for the determination of polyphenol content in beverages based on a composite material of graphene oxide decorated with Prussian blue nanocubes (rGO/PBNCs). In this method, rGO/PBNCs act as a nanoenzyme with peroxidase-like catalytic activity and produce a colorimetric product in the presence of hydrogen peroxide and tetramethylbenzidine (TMB). To verify the effectiveness of the method, we used two model standards for antioxidants: gallic acid (GA) and tannic acid (TA). The method validation included a comparison of the performance of a natural enzyme and an artificial one (rGO/PBNCs) and two polyphenols in the analysis of commercial beverage samples. After optimization, a pH of 4, ambient temperature (22 °C), a reaction time of 2 minutes and an rGO/PBNCs concentration of 0.01 μg mL-1 were found to be the most favorable conditions. The detection limits obtained were 5.6 μmol L-1 for GA and 1.5 μmol L-1 for TA. Overall, rGO/PBNCs offer advantages over natural enzymes in terms of stability, versatility, scalability and durability, making them attractive candidates for a wide range of catalytic and sensory applications.

Mn2+-doped hollow mesoporous Prussian blue nanocubes for tumor synergistic therapy

To leverage the exceptional biomedical properties of Prussian blue (PB), we synthesized Mn2+-doped hollow mesoporous Prussian blue nanocubes (Mn-HMPB) via a straightforward ion-exchange method to enhance the photothermal/chemo synergistic therapeutic efficiency. The precise modulate of nanostructures of Mn-HMPB with notable photothermal conversion efficiencies and quercetin (QUE) loading capacities. Introducing Mn2+ doping in Mn-HMPB enables pH-responsive drug release under different physiological conditions. The systematic in vitro experiments have verified that the drug release and NIR irradiation mediated by Mn-HMPB@QUE nanocubes significantly inhibit the proliferation of Hela cells, resulting in an exceptional synergistic therapeutic outcome compared to single Mn-HMPB nanocubes or free QUE. Moreover, Mn-HMPB nanocubes exhibits outstanding biocompatibility and stability in psychological environment, positioning it as a reliable nanomedicine candidate. In conclusion, this study findings demonstrate the effective inhibition of growth and metastasis of Hela cells by Mn-HMPB@QUE nanocubes, exhibiting their potential as a versatile nanoplatform for tumor therapy.

Bidirectional Confined Redox Catalysis Manipulated Quasi-Solid Iodine Conversion for Shuttle-Free Solid-State Zn-I2 Battery.

Electrochemically reversible conversion of I2/I- redox couple in a controllable iodine speciation manner is the eternal target for practical metal-iodine batteries. This contribution demonstrates an advanced polyiodide-free Zn-I2 battery achieved by the bidirectional confined redox catalysis-directed quasi-solid iodine conversion. A core-shell structured iodine cathode is fabricated by integrating multiporous Prussian blue nanocubes as a catalytic mediator, and the polypyrrole sheath afforded a confinement environment that favored the iodine redox. The zincate Znx+1FeIII/II[Fe(CN)6]y has substantially faster zinc-ion intercalation kinetics and overlapping kinetic voltage profiles compared with the I2/ZnI2 redox, and behave as a redox mediator that catalyze reduction of polyiodides via chemical redox reactions during battery discharging and an exemplary reaction is Zn(I3)2+2Znx+1FeII[Fe(CN)6]y=3ZnI2+2ZnxFeIII[Fe(CN)6]y,ΔG=-19.3kJmol-1). During the following recharging process, the electrodeposited ZnI2 can be facially activated by iron redox hotspots, and the ZnxFe[FeIII/II(CN)6]y served as a cation-transfer mediator and spontaneously catalyze polyiodides oxidation (Zn(I3)2+2ZnxFe[FeIII(CN)6]y=3I2+2Znx+1Fe[FeII(CN)6]y,ΔG=-7.72kJmol-1), manipulating the reversible one-step conversion of ZnI2 back to I2. Accordingly, a flexible solid-state battery employing the designed cathode can deliver an energy density of 215Wh kgiodine -1.

Enhanced Photothermal Activity of NanoconjugatedSystem via Covalent Organic Frameworks as the Springboard.

The development of nanomaterials with high photothermal conversion efficiency has been a hot issue. In this work, a novel photothermal nanomaterial is synthesized using Prussian blue nanocubes (PBNCs) as the photothermal active substance and covalent organic framework (COF) as the substrate. The as-prepared COF@PBNCs show a high photothermal conversion efficiency of 59.1%, significantly higher than that of pure PBNCs (32.5%). A new circuit path is generated with the combination of COF, which prevents the direct combination of thermal electrons and holes, as well as enhances the nonradiation transition of PBNCs. Besides, the imine groups on COF as the coordination and reduction agent allow the in situ growth of PBNCs, and the dense micropores of COF as the ideal heat conduction channels can also be the potential factors for the enhanced photothermal property. The photothermal property of COF@PBNCs is further used in the construction of immunosensor for the detection of furosemide (FUR). With the help of handheld thermal imager, the concentration of FUR can be easily read, thus shedding a new light in the construction of visual sensor for simple and low-cost point-of-care testing.

Photothermal and Catalytic Performance of Multifunctional Cu-Fe Bimetallic Prussian Blue Nanocubes with the Assistance of Near-Infrared Radiation.

Copper and iron are the basic metal elements that have attracted much attention in industry. Prussian blue (PB) is a significant class of metal-organic frameworks (MOFs); however, the lack of such linkages between the structure and properties, as well as properties differences, limits their potential applications. In this paper, the Cu-based Prussian blue nanocubes with and without Fe doping were synthesized. With the increasing reaction time, the morphology of the Cu-based Prussian blue nanocubes without Fe doping (PB:Cu NCs) changes from cuboidal to circular, and finally grows back to cuboidal. However, Cu-based Prussian blue nanocubes with Fe doping (PB: CuFe NCs) grow directly from the cube and eventually collapse. The nanocubes show a notable red shift with the tunable spectra from 400 nm to 700 nm. Compared with PB: Cu NCs, the PB: CuFe NCs have higher temperature rise under 808 nm irradiation and better photothermal efficacy. The catalytic efficiency of PB: CuFe NCs changes with the pH and reaches its maximum value of 1.021 mM with a pH of 5.5. The enhanced catalytic reaction by the near-infrared radiation plasmonic photothermal effect is also confirmed. This work highlights the potential of the developed PB: Cu and PB: CuFe NCs for photothermal-enhanced co-catalysis nanomaterials.

Novel electrochemical urea biosensor employing gold nanosphere-decorated Prussian blue nanocubes

Novel electrochemical urea biosensor employing gold nanosphere-decorated Prussian blue nanocubes

Hollow Nanoporous Prussian Blue Nanocubes Enriched with Cu2+ and Loaded with Quercetin for Synergistic Tumor Therapy

Combination therapy based on Prussian blue nanoparticles (PB NPs) is particularly effective in cancer because of their outstanding photothermal properties and excellent biocompatibility. However, the application of PB in tumor therapy is hindered by their poor reactive oxygen species (ROS) production capacity, and low drug loading rate. More importantly, PB NPs could activate heat shock proteins (HSPs) in photothermal therapy, which induces the self-protection of tumor cells. Structural and functional modulation improved antitumor efficacy by enhancing reactive oxygen species (ROS) production and inhibiting heat shock protein expression. In this work, we synthesized Cu2+-enriched hollow nanoporous PB nanocubes (Cu-HNPB NCs) that enhanced the •OH generating capability of PB as Cu hybridization increases the oxidation potential of PB. Moreover, in the tumor milieu, Cu2+ release depletes glutathione (GSH) and converts to Cu+, further improving ROS levels. Excess ROS amplifies cellular oxidative stress and destroys the heat-induced formation of HSPs, thus breaking the self-protection mechanism of tumor cells. To enhance the therapeutic efficiency, quercetin (QUE) was loaded in Cu-HNPB NCs (Cu-HNPB@QUE NCs). Surprisingly, the large specific surface area (127.79 m2 g–1) endowed Cu-HNPB NCs with ultrahigh loading capacity (57.25%). As the nanoplatform enhances synergistic therapeutic effects, this study provides a new approach for designing multimodal combination nanomedicine.

Graphene oxide laminates intercalated with Prussian blue nanocube as a photo-Fenton self-cleaning membrane for enhanced water purification

The separation performance of nano-laminated graphene oxide membranes is heavily decided by their nanoscale structures. Antifouling properties of the membrane have an influence on membrane performance significantly beside tuning 2D GO interlayer spacing. Herein, we intercalated a photo-Fenton catalytic Prussian blue nanocubic into graphene oxide laminates to fabricate a GO/PB membrane through a simple vacuum filtration process, the 2D nano-channel was characterized and simulated by SEM and Molecular dynamics (MD) simulations. The PB nanocubic can not only serve as nano pacers to prevent the restacking of GO nanosheets and adjust 2D nanochannels, but endow the membrane with photo-Fenton self-cleaning function. As a consequence, the GO/PB membrane presents a high separation efficiency (∼100% dye rejection), and the water permeability is nearly 2 times higher than that of the pristine GO membrane under synergistic separation and photo-Fenton self-cleaning processes. Scavenging experiments and characterization results indicated the active species of h+ and ·OH play a major role in the degradation process. In addition, the GO/PB membrane exhibited outstanding antifouling performance and reusability, retaining high dye removal efficiency (>95%) and high water-permeability (∼132.7 Lm−2h−1bar−1) after three separation cycles with photo-Fenton treatment in each cycle. Moreover, full-scale zebrafish toxicology assays have indicated that the dye stock solution exerted significant toxic effects on zebrafish with increased mortality and malformations compared to the control, while the filtrate induced negligible toxic effects. Overall, this work reveals the dual roles of PB as nanospacers and photo-Fenton catalytic nano-filler, providing a new concept for the development of hybrid nanolaminated membranes for water purification and textile wastewater treatment.

Quercetin@Gd3+ doped Prussian blue nanocubes induce the pyroptotic death of MDA-MB-231 cells: combinational targeted multimodal therapy, dual modal MRI, intuitive modelling of r1-r2 relaxivities.

Quercetin (Qu), a potential bioflavonoid has gained considerable interest as a promising chemotherapeutic drug which can inhibit the proliferation of triple-negative breast cancer (TNBC) cells due to its regulation of the expression of tumor-suppressor gene metastasis and antioxidant property. Notably, Qu exhibits a very negligible cytotoxic effect on normal cells, even with high-dose treatment, while it is shows high affinity to TNBC. However, the efficiency of Qu is limited clinically due to its poor bioavailability, caused by its low aqueous solubility (2.15 μg mL-1 at 25 °C), rapid gastrointestinal digestion and chemical instability in alkaline and neutral media. Herein, polydopamine (PDA)-coated, NH2-PEG-NH2 and hyaluronic acid (HA)-functionalized Gd3+-doped Prussian blue nanocubes (GPBNC) are reported as a multifunctional platform for the codelivery of Qu as a chemotherapeutic agent and GPBNC as a photodynamic (PDT) and photothermal (PTT) agent with improved therapeutic efficiency to overcome theses barriers. PDA, NH2-PEG-NH2 and HA stabilize GPBNC@Qu and facilitate bioavailability and active-targeting, while absorption of near infrared (NIR) (808 nm; 1 W cm-2) induces PDT and PTT activities and dual T1-T2-weighted magnetic resonance imaging (MRI) with high relaxometric parameters (r1 10.06 mM-1 s-1 and r2 24.96 mM-1 s-1 at a magnetic field of 3 T). The designed platform shows a pH-responsive Qu release profile and NIR-induced therapeutic efficiency of ∼79% in 20 minutes of irradiation, wherein N-terminal gardermin D (N-GSDMD) and a P2X7-receptor-mediated pyroptosis pathway induces cell death, corroborating the up-regulation of NLRP3, caspase-1, caspase-5, N-GSDMD, IL-1β, cleaved Pannexin-1 and P2X7 proteins. More interestingly, the increasing relaxivity values of Prussian blue nanocubes with Gd3+ doping have been explained on the basis of Solomon-Bloembergen-Morgan theory, considering inner- and outer-sphere relaxivity, wherein crystal defects, coordinated water molecules, tumbling rate, metal to water proton distance, correlation time, magnetisation value etc. play a significant role. In summary, our study suggests that GPBNC could be a beneficial nanocarrier for theranostic purposes against TNBC, while our conceptual study clearly demonstrates the role of various factors in increasing relaxometric parameters.

A dual-color fluorescent biosensing platform based on amine functionalized 3D copper Prussian blue nanocubes and exonuclease I activity for simultaneous detection of kanamycin and streptomycin

A dual fluorescent oligosensor was fabricated to detect kanamycin (KAN) and streptomycin (STR).

Glutathione depletion-mediatedin situtransformation of Prussian blue nanocubes for enhanced tumor-specific imaging and photoimmunotherapy

This work provides a novel glutathione depletion-mediatedin situtransformation strategy for enhanced tumor-specific imaging and photoimmunotherapy.

Disposable label-free electrochemical immunosensor based on prussian blue nanocubes for four breast cancer tumor markers

A compact and low-cost multi-electrode array (MEA) is presented, comprising four working electrodes with shared reference and auxiliary electrodes. Prussian blue was electrodeposited on the MEA using chronoamperometry with a positive potential of 0.3 V. Prussian blue nanocubes (PBNCs) were formed, which were observed using scanning electron microscopy. The precision of the four working electrodes was demonstrated using ferric/ferro cyanide (RSD <5.8%). The surface roughness of the working electrodes of the fabricated MEA was investigated by atomic force microscopy and compared with that of a commercial MEA. The PBNCs were the platform for a label-free immunosensor that detected four breast cancer tumor markers (CEA, CA125, CA153, and CA199) using specific antibodies. The processes of antibody immobilization were investigated using cyclic voltammetry and electrochemical impedance spectroscopy. The immunosensor was evaluated using real human serum samples, yielding acceptable recoveries (95.1–104.1%, RSD < 3.9) for the four tumor markers. These findings confirmed that our label-free immunosensor based on PBNCs could be a promising device for point-of-care testing and could pave the way for the establishment of new platforms for the screening of various breast cancer tumor markers.

A hybrid of NiCo-PBNCs nano-composite supported two-dimensional molybdenum disulfide as excellent peroxidase mimics for colorimetric glucose detection

The anchoring of two-dimensional nanosheets (2D) with Prussian blue nanocubes has exhibited the advantage of high electron conductivity and better catalytic application. Herein, we reported the successful fabrication of molybdenum disulfide-supported Prussian blue nanocubes (MoS2@NiCo-PBANCs) and compared the bare MoS2 and NiCo-PBNCs for effective peroxidase, such as catalytic activity, which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by the generation of H2O2, ensuring the appearance of blue colored solution. The MoS2@NiCo-PBANCs exhibited an excellent colorimetric sensing performance toward glucose in urine samples. Therefore, the present method demonstrates the high sensitivity, selectivity, simplicity, and fast colorimetric sensing ability for H2O2 and glucose detection. The synergistic effect of MoS2@NiCo-PBNCs reveals lower detection limits of 0.0075 µM and 0.01 µM for H2O2 and glucose, respectively. The exclusive structural morphology, excellent stability, large surface area, and high catalytic property have been utilized to achieve the closer interaction of glucose oxidase and TMB with MoS2@NiCo-PBANCs to oxidize glucose to gluconic acid. Overall, we utilized the MoS2@NiCo-PBNCs as a novel composite nanomaterial for the application of H2O2 and glucose peroxidase-like mimics in the urine sample.

A Non‐Enzymatic and Electrochemical‐Based Sensor using a Prussian Blue‐Gold Nanoparticle‐Reduced Graphene Oxide Ternary Nanocomposite for Efficient Hydrogen Peroxide Detection.

AbstractAn electrochemical sensor has been developed using PBNCs/AuNPs/RGO ternary nanocomposite, in which gold nanoparticles (AuNPs) are deposited on Prussian blue nanocubes (PBNCs) and reduced graphene oxide (RGO) using two‐step in‐situ synthetic approach. The synthesized ternary nanocomposite shows high catalytic activity towards H2O2 sensing due to the synergistic effect of gold nanoparticles and RGO. The material was prepared by the co‐precipitation method, and the morphology of the synthesized composites was characterized using X‐Ray diffraction (XRD), scanning electron microscopy (SEM), and High‐resolution Transmission electron microscopy (HR‐TEM). Electrochemical measurements were performed by modifying glassy carbon electrodes (GCE) with bare PBNCs, PBNCs/RGO, and PBNCs/AuNPs/RGO for low‐level detection of H2O2 using Cyclic Voltammetry (CV) and Chronoamperometry. The interference property was analyzed using ascorbic acid (AA), uric acid (UA), and glucose as interfering agents. The modified electrode shows high sensitivity of 5.47 nA/nM and a limit of detection of 260 pM for H2O2 at (S/N=3). This electrochemical sensor observed a linear range of detection from 0.66 nM to 10.53 nM. The proposed sensor exhibits good stability, better selectivity, and a picomolar concentration detection limit for H2O2.