Sour Cherry Research Articles

Nutritional Compounds to Improve Post-Exercise Recovery

The metabolic and mechanical stresses associated with muscle-fatiguing exercise result in perturbations to bodily tissues that lead to exercise-induced muscle damage (EIMD), a state of fatigue involving oxidative stress and inflammation that is accompanied by muscle weakness, pain and a reduced ability to perform subsequent training sessions or competitions. This review collates evidence from previous research on a wide range of nutritional compounds that have the potential to speed up post-exercise recovery. We show that of the numerous compounds investigated thus far, only two-tart cherry and omega-3 fatty acids-are supported by substantial research evidence. Further studies are required to clarify the potential effects of other compounds presented here, many of which have been used since ancient times to treat conditions associated with inflammation and disease.

Influence of Climate Change on the Phenological Stages at the Sour Cherry Genotypes

The present study establishes the influence of changes in climatic conditions on the development of ten sour cherry cultivars existing in the sour cherry germplasm fund from Research Station for Fruit Growing Iaşi, Romania. The first four phenological stages were taken in account: bud swelling (code 51 BBCH), bud burst (code 53), beginning of flowering (code 61) and end of flowering (code 69) over three different periods of time (2006-2008, 2009-2011 and 2019-2021). Were registered the number of days, the sum of active temperature (SAT), growing degree-days (GDD) as well as the correlation coefficient between the number of days and the sum of active temperature between phenological developmental stages, buds swelling and bud burst, bud burst and beginning of flowering and from beginning of flowering to the end of flowering. The results obtained in the three time periods (from 2006 to 2021) have statistically significant differences and were recorded especially at the phenophases of flowering. The study determined that the higher annual average temperature was (from 10.45℃ to 11.69℃), the more significant were the statistically differences. Thereby, phenological changes were a reaction to temperature what can be assumed also with the results of the present study.

Weather Conditions, Orchard Age and Nitrogen Fertilization Influences Yield and Quality of ‘Łutówka’ Sour Cherry Fruit

Nitrogen fertilization is a basic agrotechnical orchard treatment, and affects quality and size of the crops. The aim of this study was to assess the impact of nitrogen fertilization on the yield and quality of sour cherry fruit of the ‘Łutówka’ cultivar. The course of climatic conditions in relation to the timing of flowering and fruiting was analyzed. The trials were carried out in three different experimental sour cherry orchards (quarters) with different planting dates (1999, 2001 and 2002) located at the experimental orchard of the Poznań University of Life Sciences in Poland. In each orchard, three levels of fertilization were used: control without fertilization, fertilization at a dose of 60 kg N ha−1 and fertilization at 120 kg N ha−1. Increasing the dose of N did not have a significant effect on sour cherry yield. However, it resulted in a reduction of fruit weight, extract content, acidity and TSS/TA ratio, whereas the brightness (L*) and redness (a*) of the fruit surface color increased. Temperature and precipitation had the greatest influence on the course of flowering, fruiting and fruit ripening. Particularly important was the course of climatic conditions at the beginning of the growing season, especially during flowering, when there is a high risk of spring frosts. The timing of flowering and fruiting was correlated with the sum of active temperatures. The greatest relationship for the flowering date was found for SAT (sum of active temperatures) when the base temperature equals 11 °C and the temperature for the harvest date equals 9 °C.

Flora of the former Kolokolnikov estate (Tyumen)

The article presents for the first time data on the species diversity of vascular plants growing on the territory of the former estate of the Kolokolnikovs, which has a history of formation for a century and a half and is today a place of rest for the citizens of Tyumen. In 2021, most of the study area was classified as a natural monument of regional significance «Lesopark (Parkland) Zatyumensky». For the spring-summer period 2021, 182 species of vascular plants belonging to 136 genera, 56 families were identified. Among the leading families: Asteraceae (22 species), Rosaceae (21), Poaceae (19), Fabaceae (14), Brassicaceae (9), Salicaceae (8), Caryophyllaceae (7), Apiaceae (6), Ranunculaceae (5), which accounted for 61 %. The arboreal layer is dominated by Betula pendula and Tilia cordata, and the grass-shrub layer is dominated by Aegopodium podagraria, Centaurea scabiosa, Geranium sibiricum, Glechoma hederacea, and Poa pratensis. On the territory there are species listed in the Red Book of the Tyumen region: Tilia cordata and Cerasus fruticosa (Prunus fruticosa). In a comparative analysis with herbarium funds, 33 plant species were not identified in the modern flora of the study area. According to the biomorphological analysis according to the system of life forms of K. Raunkier, most plants belong to hemicryptophytes (40.7 %), I. G. Serebryakova - long-rhizomatous polycarpics (21.5 %), O. V. Smirnova et al. - mono-centric type (52.2 %).

The floristic quality assessment index as ecological health indicator for forest vegetation: A case study from Zabarwan Mountain Range, Himalayas

Evaluations ecosystem health-based field studies can assist decision-makers in formulating more targeted conservation policies to better manage ecosystems and landscape. To analyze forest characteristics in the Zabarwan Mountain Range we used the Floristic Quality Assessment Index (FQAI) technique to evaluate the ecosystem health status of the vegetation. According to our findings, the forest area showed an FQAI rating of 61.41. The FQAI ranked 54 of the plant species growing in the forest region at 0, 30 species at 1–3, 51 species at 4–6, 30 species at 7–8, and 17 species at 9–10. Of the 54 alien species ranking 0, thirteen species were invasive, 36 were naturalized, and five were casual. Amoung the forest types, the Broadleaved forest harbored most alien species (35%) and the scrub forest the least (6%). Based on habitat-wise distribution, the largest (38%) species pool was observed in natural forest habitats, while human-modified habitat types harbord 51% of species. Roadsides exhibited the by far highest alien species number (45%) together with other anthropogenic habitats. The investigation of indicator species revealed a separation between the various ranking groups, as evidenced by high indicator values. Important alien indicator species ranking 0 were i.e., Aesculus indica, Ailanthus altissima, Celtis australis, Daucus carota, Poa bulbosa, Prunus armeniaca, Prunus cerasus, Quercus robur, and Salix alba, which showed a significant p-value for indicator species analysis. The findings of this study provide a method for measuring vegetation communities' responses to alien invasions and as a result can serve for developing management methods to preserve ecosystems, particularly in protected areas, from this biodiversity threat.

Development of FT-NIR Technique to Determine the Ripeness of Sweet Cherries and Sour Cherries

The FT-NIR technique was used for the rapid and non-destructive determination of sweet cherry and sour cherry ripeness. Titratable acidity (A), water-soluble total solids (SSC), total anthocyanin (TA) content and calculated maturity index (SSC/A = MI) were used as reference values. PLS correlations were validated by seven-fold cross-validation (RMSECV for different parameters: DM = 1.25%, w/w; A = 0.14%, w/w; SSC = 0.97%, w/w; TA = 17.5 g/100 mL; MI = 1.66) and test-validation (RMSEP for different parameters: DM = 1.46%, w/w; A = 0.19%, w/w; SSC = 0.99%, w/w; TA = 17.5 g/100 mL; MI = 1.59). Different discriminant analyses and support vector machine (SVM) classifications were performed for each parameter. The quadratic discriminant analysis (QDA) was found to be the best pattern recognition method. A maturity degree (MD) was developed based on the reference values, which classified the samples into mature and immature categories with an accuracy of 98.44%.

Effects of Cherry (Prunus cerasus L.) Powder Addition on the Physicochemical Properties and Oxidation Stability of Jiangsu-Type Sausage during Refrigerated Storage.

Effects of different levels (1%, 3% and 5%) of cherry powder on the physiochemical properties and antioxidant activity of Jiangsu-type sausages were investigated at 4 °C for 30 days. The results show that the sensory evaluation values and physicochemical properties of the sausages had no significant differences compared to the control group when cherry powder addition was 1%, and the alcohols, aldehydes and esters were increased after the addition of cherry powder improved the flavor of sausages. However, higher concentration of cherry powder (3% and 5%) exerted adverse influences on sensory evaluation values and physicochemical properties of sausages compared with the control. The addition of cherry powder could better inhibit lipid and protein oxidation of sausages, and the cherry powder concentration has a positive correlation with its effect on the inhibition of lipid and protein oxidation. In addition, cherry powder could effectively control TVB-N values of sausages during chilled storage. All these results indicate that 1% cherry powder could not only guarantee the physicochemical properties of sausages, but also inhibited the oxidation of sausages during chilled storage.

The use of different LEDs wavelength and light intensities for in vitro proliferation of cherry rootstock: influence on photosynthesis and photomorphogenesis

Plant growth and development are strongly influenced by light quality conditions in their environment. In this study, in vitro shoot proliferation, photosynthetic pigments, leaf anatomy and photosystem II photochemistry of Gisela 6 cherry rootstock (Prunus cerasus x Prunus canescens) were investigated. The culture medium used was the MS (Murashige and Skoog) containing 4.4 μΜ 6-benzyladenine (BA), 0.05 μΜ α-naphthalene acetic acid (NAA) and 0.3 μM gibberellic acid (GA3) (30 days, 16 h photoperiod, 22 ± 2 °C). Initially, two different light wavelength absorption spectra: 400–700 nm provided by white fluorescent light lamps (mainly blue–green, BG at equal ratio 1:1; WFL-40) and, 430–690 nm provided by two Light Emitting Diodes (LED) types, differing in the spectral ratio of multiple light emitting wavelengths [LED2-40 (blue–red, BR) and LED3-40 (blue–green–yellow–orange–red, BGYOR)] were studied, under 40 μmol m−2 s−1 of light intensity. Following that, three LED intensities-20 (LED1-20), 40 (LED2-40), and 120 μmol m−2 s−1 (LED4-120) were investigated under BR spectrum. WFL-40 exhibited non-significant differences with LED2-40 (shoot number 4.25, proliferation rate 4.30, shoot height 0.91 cm). LED2-40 displayed higher fresh and dry shoot biomass, better PSII functionality, increased Chl a/b, Chl b and carotenoids content as well as decreased number of stressed explants. Leaf anatomy under LED2-40 and WFL-40 was similar. LED4-120 presented the highest rate of stressed explants, with their leaf anatomy being severely affected, as leaves had a reduced mesophyll cell density. In LED4-120, the proportion of the absorbed energy used in photochemistry (ΦPSII) was decreased whereas the increased quantum yield of non-regulated energy loss in PSII (ΦNO) could not be compensated by the increased photoprotective energy dissipation (ΦNPQ). Therefore, LED2-40 is proposed as the most beneficial for growing cherry rootstock Gisela 6 plantlets in vitro.

The Effect of Adding Sour Cherry Concentrate to The Usual Treatment of Attention Deficit Hyperactivity Disorder In 6 To 12 Years Old Children

Introduction: This study aimed to evaluate the efficacy of sour cherry concentrate in symptoms of Attention Deficit Hyperactivity Disorder (ADHD). Methods: In a randomized clinical trial, 70 children with ADHD referred to the psychiatric clinic of Ibn-e-Sina Hospital, Mashhad, Iran, in 2021 were selected. Patients were randomly divided into two groups. The intervention group received 75 ml of sour cherry concentrate twice daily in addition to the routine treatment for 2 weeks. The patients were evaluated using The Conner’s Parent Rating Scale (CPRS) before and after the study by a psychiatry resident. Results: Based on the findings, no significant differences were observed between the groups in change percent of Cognitive impairments (p = .317), Hyperactivity/impulsivity (p = .525), Oppositional (p = .986), and ADHD index (p = .451). Moreover, 28.6% and 31.4% of children with ADHD consuming the concentrate presented abdominal pain, and asthenia, respectively. Conclusion: The present study did not indicate a beneficial effect of sour cherry concentrate on symptoms of ADHD.

Investigation of the use of various fruit juice concentrates instead of corn syrup in marshmallow type products: A preliminary study

In this study, the effects of using various fruit juice concentrates (pomegranate, grape and sour cherry) instead of corn syrup in marshmallows on the physico-chemical, textural and microstructural properties of the products were investigated. Experimental points of the study were determined by D-optimal mixture design, and accordingly, special cubic, linear and quadratic models were used for the effects on physico-chemical, color and texture parameters according to independent variables. Considering the results, pH, water activity and density values of the marshmallow samples were found to be in the range of 2.54–3.21, 0.48–0.55 and 0.44–0.66 g/mL, respectively. As expected, the use of fruit juice concentrates affected the visual properties of marshmallows, and in particular sour cherry and pomegranate juice concentrates caused a positive increase in the +a* values of the samples. According to the texture profile analysis, the hardness, springiness, cohesiveness, gumminess and resilience values of the samples were determined as 6.43–10.5 N, 0.84–97, 0.74–0.90, 5.18–8.22 g, 0.34–0.81, respectively. R2 values of investigated parameter models were determined between 0.4971 and 0.8901. It was concluded that fruit juice concentrates can be used instead of corn syrup in marshmallows to meet consumer demands and expectations. Finally, it is thought that it will be useful to investigate the effects of various hydrocolloids on the color, texture stability and sensory properties of the products with further studies using fruit juice concentrates in marshmallow production.

Mathematical modeling of continuous induction heating of sour cherry juice

Induction heating is a noncontact heating method that enables heating of ferromagnetic materials. In this study, continuous induction heating system was custom set up and experimental and numerical results were compared. Sour cherry juice was heated to a target temperature value (75°C) at three different flow rates (200–250-300 ml/min) and three different set pipe wall temperatures (80–90–100°C) in experimental study. COMSOL Multiphysics 5.2 was used to solve mathematical models and visualize temperature distribution during heating and flowing. Magnetic field, heat transfer, and laminar flow physics paired each other in the model. It was concluded that the time-dependent variation of the sour cherry juice output temperature was successfully predicted by the model developed for induction process conditions. The developed models could be used to estimate process time, temperature history of materials, and effect of flow rate on heating rate any time during the continuous induction heating process. Practical applications Thermal processes applied in the food industry are aimed to inactivate the microorganisms in the food, to protect the nutrient content, and to have high energy efficiency. Conventional heating methods have a negative effect on the nutrient content of foods and the energy efficiency of the heating process due to long processing times. The advantages of induction heating are that it is a novel heating method, highly energy efficient, and performs heating process in a short time. In this study, a laboratory-scale continuous induction heating system to heat a liquid food product was established and numerical modeling of the heating process was performed. The obtained model will be an important data for installation of the continuous induction heating systems, scale-up in the continuous induction heating systems, and prediction to effects of the process parameters.

COMPARISON OF THE INFLUENCE OF DIFFERENT FRUIT DRYING METHODS ON THE CONTENT OF SELECTED BIOACTIVE SUBSTANCES

The aim of the work was to evaluate the effect of hot air and infrared drying on the content of total polyphenols and anthocyanin dyes in selected types of small fruits. In the work we used chokeberry (Aronia melanocarpa Michx.) variety Nero, elderberry (Sambucus nigra L.) variety Haschberg, sour cherry (Prunus cerasus L.) variety Fanal, red currant (Ribes rubrum L.) variety Lake, black currant (Ribes nigrum L.) variety Triton, cherry elaeagnus (Elaeagnus multiflora Thunb), raspberry (Rubus idaeus L.) variety Heritage and blueberry (Vaccinium corymbosum L.) variety Bluecrop. Drying was performed in a Concept S1060 hot air dryer and a Yden CI IR D5 infrared dryer. The analysis of the content of anthocyanin dyes was performed by the pH differential method, the determination of the total content of polyphenols was performed by the Folin - Ciocalteu method. By analyzing fresh fruits, we found the highest content of anthocyanin dyes in the fruits of chokeberry (3036.97 mg.100 g-1 DM) and the lowest in the fruits of cherry elaeagnus (83.86 mg.100 g-1 DM). The total polyphenol content ranged from 726.07 mg GAE.100 g-1 dry matter (cherry elaeagnus) to 7895.61 mg GAE.100 g-1 DM (chokeberry). After drying in a hot air dryer, the content of anthocyanin dyes decreased by 62.20% (in elderberry) to 72.52% (in cherry elaeagnus) and the content of total polyphenols by 45.16% (in chokeberry) to 53.34% (in red currant). After drying in an infrared dryer, the decrease in the content of anthocyanin dyes was 50.41% (in elderberry) to 71.22% (in cherry elaeagnus) and total polyphenols 41.05% (in chokeberry) to 47.89% (in sour cherry).

EFFECT OF FORMULATION VARIABLES ON SENSORY QUALITY OF THERMO-REVERSIBLE FRUIT FILLING FOR FROZEN SEMI-FINISHED BAKERY PRODUCTS: MODELLING AND OPTIMIZATION

Development of a food product requires integrated scientific and professional knowledge on the technological process, the final product and, the quality of the ingredients and their interaction. This research examined the effect of formulation variables on the sensory quality of a thermo-reversible sour cherry fruit filling, as well as the modelling and optimization of the variables for achieving the quality suitable for filling frozen semi-finished bakery products in industrial conditions. Each formulation for 15 model-samples was successively corrected, to achieve the appropriate sensory quality of the filling in finished products after baking. Their quality was evaluated by descriptive sensory and physico-chemical methods of analysis and compared. The basic formulation for the filling was defined after the successive production of 7 model-samples in laboratory conditions, their use in industrial conditions (puff pastry filling, shaping, quick freezing, storage for at least 24 hours at -18°C, baking and cooling to room temperature) and analysing their quality. Further formulation optimization included selecting: the type and amount of fruits (sour cherry, apple), food additives and cherry flavour for 8 new model-samples. The formulation and ingredients for the fruit filling production (30% sour cherry fruit, 30% apple puree, 58% sugar, 2% starch, 1% pectin, 0.03% firming agent E509, 0.03% acidity regulator E331, 0.025% cherry flavour) and quality parameters (dry matter 65%, acidity 0.85%, pH = 3.2-3.4) were defined. The fruit filling had appropriate sensory quality before and after baking of filled puff pastry: clear cherry-red colour, moderately firm consistency and stable volume, rich fruity aroma, and refreshing sweet-sour taste. The results also confirmed that the product was suitable for manual or machine filling of raw dough.

Characterisation of the little cherry virus 1 isolate infecting almonds in the Czech Republic

Little cherry virus 1 (LChV1) of the genus Velarivirus is an important pathogen associated with the little cherry disease. It infects various species of the genus Prunus, mainly the sweet cherry and the sour cherry. Recently, plums and apricots have also been reported as natural hosts. In the present work, the LChV1 isolate causing chlorotic mosaics in almonds is characterised. The nearly complete (+)ssRNA genome sequence of the Alm138 isolate, obtained by Sanger sequencing, is 16 878 nt long showing a typical velarivirus structure with 8 ORFs. Among them, the taxonomically important ORFs, ORF1a/1b encoding the polyprotein is 8 421 nt long, with a 0/+1 frameshift position at 6 923; ORF3 encoding the heat shock protein HSP70h is 1 656 nt long, and ORF6 encoding the coat protein duplicate is 1 989 nt long. The genome sequence showed its highest identity with LChV1 isolates Apr184R (96.2%), 19SP003B (92.2%) and Kyoto-2 (92.1%). A similar situation was also found for the ORF1, HSP70h, and CP nucleotide and amino acid sequences. A phylogenetic analysis identified Alm138 as a member of the G5 phylogenetic group, supporting its close relationships with the Czech Apr184R apricot isolate and the other members of this group. This report describes the first little cherry virus 1 isolate infecting almonds and confirms the presence of isolates of the G5 phylogroup within Europe.

Screening of cherry leaf roll virus in Sambucus nigra plants in Ukraine

Goal. Cherry leaf roll virus (CLRV) infects naturally a wide range of herbaceous and woody plants, different species of trees, shrubs, horticultural, ornamental, weed plants and causes significant economic losses in many hosts and countries. CLRV on elderberry species was reported in Europe, USA, Iran and North America. Recently this virus has been detected in sour and sweet cherry fruit orchards in Ukraine. Testing of other hosts than Prunus was performed in Ukraine only recently. The aim of the study was to test the black elderberry plants (Sambucus nigra L.). for the presence of CLRV in the Poltava region and Kyiv regions.
 Methods. Sampling was carried out in the summer–autumn period in 2019—2021 in the territories of the Poltava and Kyiv regions and in Kyiv city. Samples of healthy elderberry plants were also analyzed. Visual diagnostics, enzyme-linked immunosorbent assay in DAS-ELISA modification, total RNA extraction, RT-PCR with primers for a 412 bp fragment of the 3’ untranslated region of the CLRV genome, and statistical data analysis were used in this research. PCR products were separated on an 1.5% agarose gel. Commercial CLRV preparations were used for positive controls in DAS-ELISA.
 Results. Thirty three elderberry samples with symptoms of leaf rolling and mosaics of varying degrees were selected in the territory of the Poltava and Kyiv regions and in Kyiv city in 2019 and 2021 and used in the study. ELISA and RT-PCR results showed that 82% of the tested black elderberry samples were infected by CLRV.
 Conclusions. The presence of CLRV, its harmfulness for elderberry plants, and Sambucus potential to serve as a reservoir for the virus indicate the necessity of testing a wider range of plant species for сherry leaf roll virus in Ukraine.

Micronization enhanced extractability of polyphenols and anthocyanins in tart cherry puree

Tart cherries (Prunus cerasus L.) are the richest in phenolic compounds, among all the members of drupe family of fruits. To reduce the inherent sourness of the cherries, thermal processing or filtration steps are used. These processes lead to changes in pH, and exposure to temperature enhances oxidation leading to degradation of polyphenols. In the present study, Megatron® and high-pressure homogenization (HPH) were employed to produce micronized puree, which was analyzed for polyphenolic, flavonoid and anthocyanin contents using various assays. Both methods of micronization lead to breakage of cell wall structure facilitating enhanced release of bioactives from the cellular matrix and thus higher antioxidant release. The presence of functional groups was corroborated by FTIR spectrophotometric analysis, and reversed phase HPLC for the presence and relative amounts of various polyphenols and anthocyanins. There was a significant increase (p<0.05) of in the extractability of polyphenols with HPH (285.05 GAE μg/g) in comparison with the non-micronized samples (166.80 GAE μg/g). The changes in anthocyanins and flavonoids were also significant (p<0.05), thus confirming the impact of micronization on the antioxidant release in tart cherries.

Classification of Prunus Genus by Botanical Origin and Harvest Year Based on Carbohydrates Profile.

The 1H-NMR carbohydrates profiling was used to discriminate fruits from Rosaceae family in terms of botanical origin and harvest year. The classification was possible by application of multivariate data analysis, such as principal component analysis (PCA), linear discriminant analysis (LDA) and Pearson analysis. Prior, a heat map was created based on 1H-NMR signals which offered an overview of the content of individual carbohydrates in plum, apricot, cherry and sour cherry, highlighting the similarities. Although, the PCA results were almost satisfactory, based only on carbohydrates signals, the LDA reached 94.39% and 100% classification of fruits according to their botanical origin and growing season, respectively. Additionally, a potential association with the relevant climatic data was explored by applying the Pearson analysis. These findings are intended to create an efficient NMR-based solution capable of differentiating fruit juices based on their basic sugar profile.

Nutritional Modulation of Sleep Latency, Duration, and Efficiency: A Randomized, Repeated-Measures, Double-Blind Deception Study.

This study aimed to test the hypothesis that a novel nutritional blend composed of tryptophan, glycine, magnesium, tart cherry powder, and l -theanine enhances subjective and objective measures of sleep during free living conditions. In a randomized, repeated-measures crossover and double-blind deception design, participants ( n = 9 males and 7 females, age = 24 ± 3 yr, body mass = 69.8 ± 11.6 kg, stature = 170.8 ± 9.1 cm) completed a 3-d familiarization period, followed by 3-d intervention and placebo trials. Subjective Pittsburgh Quality Sleep Index, Core Consensus Sleep Diary, and Karolinska Sleepiness Scale survey tools, alongside objective actigraphy measures of sleep, were assessed, with daily nutritional intake, activity, and light exposure standardized between trials. Participants provided daily urine samples for assessment of targeted and untargeted metabolomes. The intervention trial reduced sleep onset latency (-24 ± 25 min; P = 0.002), increased total sleep time (22 ± 32 min; P = 0.01), and increased sleep efficiency (2.4% ± 3.9%; P = 0.03), while also reducing morning sleepiness ( P = 0.02). Throughout the study, 75% of participants remained blinded to sleep assessment as a primary outcome measure, with 56% subjectively indicating improved sleep during the intervention trial. Metabolomic analysis highlighted several significantly altered metabolomes related to sleep regulation between trials, inclusive of 6-sulfatoxymelatonin, d -serine, and l -glutamic acid. Data demonstrate that using the proposed blend of novel nutritional ingredients during free living conditions reduced sleep onset latency, increased total sleep duration, and increased sleep efficiency, leading to reduced perceptions of morning sleepiness. These effects may be mediated by the upregulation of key metabolites involved in the neurophysiological modulation of the sleep/wake cycle.

Validated QuEChERS-based UHPLC-ESI-MS/MS method for the postharvest control of patulin (mycotoxin) contamination in red-pigmented fruits

The paper outlines a procedure based on ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry and QuEChERS pretreatment for the determination of patulin in selected berries and stone fruits. The mycotoxin was determined in the positive electrospray ionization mode utilizing the formation of its methanol adduct. The method was validated for 4 fruit matrices: strawberry (Rosaceae, Fragaria), raspberry (Rosaceae, Rubus), redcurrant (Rosaceae, Prunus) and sour cherry (Grossulariaceae, Ribes). The fruits were characterized by total phenolics, flavonoids, and anthocyanin contents and obtained results were within the ranges of 2.65–11.22 mg GAE/g, 1.06–4.67 mg CE/g, 0.05–1.44 mg cyd-3-glu/g, respectively. The limits of quantification ranged from 0.65 µg/kg to 3.01 µg/kg depending on the fruit type. The method was applied to 71 fruit samples collected in Poland. Analysis of moldy fruit samples has shown a high incidence of patulin contamination.

Chemopreventive effects of Prunus cerasus L. against human cancer cells & ascites mice models and its phytochemical investigation by LC-Q-TOF-MS/MS

BackgroundBerries, including cherries and their derived distinctive bioactive leads, are explored for the establishment of therapeutic supplements, owing to their versatile biological applications accompanied with less or no side effects to normal cells. The current research work aimed to identify various phytocomponents of P. cerasus fruit extract (PcMFE) and investigate its chemopreventive properties employing cellular assays coupled with experimental Ascites mice models. MethodLC-ESI-Q-TOF-MS (HRMS) approaches were used for chemoprofiling of P. cerasus methanolic fruit extract (PcMFE). After testing for scavenging capacities on DPPH, radical & lipid peroxidation, the In-vitro anticancer potential was investigated against five distinct human cancer cell lines A-549, THP-1, MCF-7, PC-3 and NCI-H322 using MTT and SRB assays. In case of in-vivo antitumor assessment, PcMFE was investigated against Ehrlich Ascites Carcinoma (EAC) and Methylcholanthrene induced Ascites (Meth-A) experimental mice models. ResultsHRMS analysis of PcMFE resulted in identification of 59 compounds belonging to diverse chemical classes. Besides significant antioxidant potential, PcMFE at 50 µg/ml dose blocked 40–100% growth of various cancer cell lines, with maximum (100% growth inhibition) against NCI-H322 showing an IC50 of 5.59 µg/ml and 5 µg/ml respectively in SRB and MTT assays. Execution of apoptosis induced by PcMFE in NCI-H322 cancer cells revealed an increased population of apoptotic cells evidenced through nuclear morphology studies. Moreover, intraperitoneal administration of PcMFE at 200 mg/kg body weight reported 72.31% and 68.69% tumor growth inhibition in EAC & Meth-A mice model, respectively. ConclusionsThe present study exhibited that P- cerasus fruit is a valuable source of diverse functional metabolites and may facilitate development of chemopreventive supplements.