Antimicrobial resistance is one of the major challenges facing the health sector. This study was aimed at investigating the antimicrobial susceptibility pattern of bacterial isolates from indoor air of public toilets in motor parks with a view of developing an antibiogram. The study sites included the public toilets in Mile 3 Motor Park, Rivers Transport Company (RTC) park and a General Motor Park in Waterlines, Port Harcourt, Nigeria. The indoor air was sampled using the plate exposure and disc diffusion techniques in determining the antimicrobial susceptibility pattern of the bacterial isolates. The bacterial isolates in the genera, Staphylococcus, Bacillus, Providencia, Pseudomonas, Escherichia, Enterobacter and Klebsiella species were identified. The antibiogram of the bacteria isolated showed that the bacterial isolates exhibited multi-drug resistant species as the isolates were resistant to more than two antibiotics. Ciprofloxacin and Tarivid are the drug of choice and recommended for treatment of infections from these study sites.