Provenance History Research Articles

Sequencing and Partial Molecular Characterization of BAB-TMP, the Babeș Strain of the Fixed Rabies Virus Adapted for Multiplication in Cell Lines.

The rabies virus is a major zoonosis that causes severe nervous disease in humans, leading to paralysis and death. The world's second anti-rabies center was established in 1888 by Victor Babeș, in Bucharest, where an eponymous strain of rabies was isolated and used to develop a method for immunization. The Babeș strain of the rabies virus was used for over 100 years in Romania to produce a rabies vaccine for human use, based on animal nerve tissue, thus having a proven history of prophylactic use. The present study aimed to sequence the whole genome of the Babeș strain and to explore its genetic relationships with other vaccine strains as well as to characterize its relevant molecular traits. After being adapted for multiplication in cell lines and designated BAB-TMP, 99% of the viral genome was sequenced. The overall organization of the genome is similar to that of other rabies vaccine strains. Phylogenetic analysis indicated that the BAB-TMP strain is closely related to the Russian RV-97 vaccine strain, and both seem to have a common ancestor. The nucleoprotein gene of the investigated genome was the most conserved, and the glycoprotein showed several unique amino acid substitutions within the major antigenic sites and linear epitopes.

Phenotypical characterization, and antibiotics susceptibility patterns of skin bacteria found in podoconiosis patients in the North West Region of Cameroon

BackgroundPodoconiosis, a non-infectious disease originating from long-term exposure of bare feet to irritant red clay soil is a lifelong, disabling disease with no specific diagnostic tool, classified into 5 stages based on the severity of leg swelling (lymphoedema). Secondary bacterial infections have been suggested to cause acute dermatolymphangioadenitis (ADLA) attacks and drive disease progression. Although the North West Region of Cameroon has a proven history of podoconiosis endemicity, the bacterial composition of lymphoedema due to this condition has not been studied. Thus, this study investigated the leg bacterial diversity of patients who suffered from the lymphoedema and their susceptibility pattern to selected antibiotics.MethodsA cross-sectional study was carried out in which podoconiosis affected and non-lymphoedema individuals living in the same community were purposively selected. Samples were collected by swabbing the skin between the toes and around the anklebone, then cultured and sub-cultured on nutrient agar to obtain pure isolates. The cultured isolates were then morphologically and biochemically classified using microscopy and analytic profile index test kits, respectively. The disk diffusion technique was used to determine antibiotic susceptibility.ResultsThirty-three participants were recruited, and 249 bacterial isolates were characterized into 29 genera, 60 species; with 30 (50%) being gram positive rods, 19 (31.7%) gram positive cocci, and 11 (18.3%) gram negative rods. Thirteen gram positive rods, fifteen gram positive cocci, and eight gram negative rods of bacterial species were found only in podoconiosis individuals among which Cellulomonas spp / Microbacterium spp. (2.8%), Staphylococcus lentus (3.3%), and Burkholderia cepacia (4.0%) dominated. 90% (90%) of the bacterial isolates were sensitive to doxycycline, whereas ampicillin had a high level of intermediate resistance, and penicillin G had the greatest resistant profile.ConclusionOur findings show that 94 (37.8%) out of 249 described bacterial isolates were exclusively found in the legs of podoconiosis individuals, and their susceptibility pattern to antibiotics was similar to that of others.

Geochemistry of the siliciclastic sediments in the Barak basin, Indo-Burma Range, India: Insights into provenance, paleoclimate, and depositional history

Sedimentation of the Paleogene and Neogene belts in the Indo-Burma Range (IBR) involved a complex tectonic system letting provenance of both the belts poorly constrained. We made an attempt to investigate provenance, tectonic setting, paleoclimate, and depositional environment of the Renji Formation (Paleogene belt) and the Bhuban Formation (Neogene belt) in the Barak basin of western Manipur employing whole-rock geochemistry and petrography. A semi-arid climatic condition has been indicated for the weathering and liberation of sediments in the source terrain. Derivation of first cycle sediments from mafic and felsic rocks with minor contribution from the metamorphic rocks is suggested but the former seems to have dominated the Renji Formation. A shift in the provenance vis-a-vis differential chemical weathering of the mafic-felsic rocks at the source is attributed. Research results also suggest supply from deformed and elevated craton, presumably the Indian craton, during the Himalayan orogeny. Sedimentation of the Renji Formation took place in a sub-oxic deltaic environment in contrast to the Bhuban Formation which developed in an oxic shallow marine mixed tidal flat environment.

Antiviral Activity of Svarnvir-IV Tablet Assayed for Activity Against SARS-CoV-2 In Vitro.

The coronavirus disease (COVID-19), caused by the virus SARS-CoV-2, has become a global pandemic in a very short time span. While several vaccines have been developed in the last year, specific treatments for CoV infection are still being explored. Thus, the situation highlights the need to develop safe and efficacious antiviral therapeutics. AyurvedicRasayanatherapy has been traditionally used in India for its holistic healing systems and proven history of empirical use. There is emerging evidence that Ayurvedic treatment methodologies and herbal medicines may be effective strategies in combating COVID-19. The present study is aimed at evaluating the antiviral and therapeutic activity of an Ayurvedic herbomineral formulation (Svarnvir-IV tablet, 450 mg) against the SARS-CoV-2 virusin vitro. A cell-based assay was conducted to evaluate the cytotoxicity of the Svarnvir-IV tablets (Aimil Pharmaceuticals, Delhi, India) for the determination of virucidal activity assessment (at 2 hours) and therapeutic activity assessment (at 1 hour, 2 hours, and 4 hours). When incubated with SARS-CoV-2 virus at 0.1 multiplicity of infection (MoI) for two hours, Svarnvir-IV tablet exhibited virucidal activity against SARS-CoV-2 with an EC50 value of 0.0058 mg/ml. It also exhibited therapeutic activity when treated with cells infected with the SARS-CoV-2 virus (0.1 MoI) for 1 hour, 2 hours and 4 hours post-infection, with an EC50 value of 0.094 mg/ml, 0.023 mg/ml, and 0.05 mg/ml, respectively. The original supporting data obtained from this study, along with existing Ayurvedic traditional information, has shown encouraging results.

The paleoenvironmental and thermal histories of the Permian Irati Formation shale in the Paraná Basin, Brazil: An integrated approach based on mineralogical and organic imprints

Mineralogical assemblages and organofacies are important sources of information to recover the paleoenvironmental and thermal histories of shale deposits. In this study, a detailed qualitative and quantitative characterization of the Permian Irati Formation (Assistência Member) shale is based on mineralogical (XRD and SEM-EDS) and organic components (TOC, Rock-Eval pyrolysis, organofacies, TAI, fluorescence and vitrinite reflectance measurements) and provides integrated data about sediment provenance, depositional environment, diagenesis, and thermal history, while supporting interpretations on the Paraná Basin (PB), Brazil, paleogeography and its correlation to the southwest Gondwana. The results revealed a prevailing type I/II kerogen, with type III kerogen being also present but mainly confined along the paleoshoreline of the PB. The dominance of fluorescent amorphous organic matter (AOM) combined with framboidal pyrite suggests microbial activity in an anoxic-dysoxic neritic-marine paleoenvironment. Additionally, common to abundant well-preserved phytoclasts, as well as the occurrence of Botryococcus braunii, indicates freshwater influx in a brackish marine depositional setting. Immature to early-oil window thermal maturities prevail across the PB, according to the organic maturation indicators. The combined analysis between the organic matter evolution with clay mineralogy, such as the occurrence of interstratified clays (e.g., I/S) and its positive correlation with depth suggest that burial diagenesis reached the transition to early catagenesis on the north, southeast, and south of the basin, attributing a shale oil potential for the Irati Formation on a regional scale. Local scale imprints of the Early Cretaceous Paraná-Etendeka Large Igneous Province (LIP), and its thermal effect in the Irati Formation shale components, are recorded as clay authigenesis (e.g., smectite webby texture and clay coating development), crystallization of minerals by low to high-grade of thermal alteration (e.g., corrensite, talc, lizardite and diopside), and by local scale gas-window maturities. Such thermal alteration, identified in the proximity to intruded sills and dykes, led to a heterogeneous organic maturation pattern with implications on shale gas and shale oil potential of the Irati Formation shale, demonstrating that these subjects in the Paraná Basin should be assessed locally.

Prevalence and characteristics of COVID-19 vaccine breakthrough infection in Upper Egypt

BackgroundInfection breakthrough after COVID-19 vaccination is a point of conflict in current literature not only because of the estimation of the potential hazards and symptoms severity but also due to establishing a vaccination policy and measuring the extent of immunity after vaccination in addition to the waning of the humoral immunity over time. To our knowledge, this is the first study to stratify the risk of post-COVID-19 vaccination breakthroughs in Upper Egypt.MethodsIn this cross-sectional observational study, we enrolled 369 vaccinated patients registered in our facility either admitted or in out-patient clinic. Patients were interviewed and any proven history of COVID-19 infection after vaccination was recorded.ResultsIn the current study, 18.97% (70 patients) of the 369 subjects enrolled in the study had COVID-19breakthrough infection. Students were the high-risk group representing 18.6% of the study subjects. Hypertension, diabetes, and cardiac disease were the most comorbidities associated with a prevalence of 15.7%, 8.6%, and 4.3% respectively. Prior to COVID-19, confirmed infection was present in 42.9% of the study group. Fever, headache, myalgia, and cough were among the most common symptoms of the post-COVID vaccination breakthrough with a prevalence of 90%, 75.7%, 84.3%, and 74.3% respectively.ConclusionBreakthrough infection after COVID-19 vaccination is not uncommon. Most cases are mild and don’t require hospitalization. All types of vaccines tested in the current study offer adequate immunity and guard against severe COVID-19 infection. We encourage the current global policy of full vaccination.Trial registrationClinicalTrials.gov. NCT05033834. Registered September 5, 2021. COVID-19 Infection in After Vaccination-Full Text View-ClinicalTrials.gov.

Cretaceous source to sink system reconstruction of northeastern Asian continental margin: Insight from integrated detrital geochronology in NE China

The Cretaceous sedimentary successions in NE China are largely incomplete as the basins are inverted and younger strata are eroded. Except for the Songliao Basin, whose depositional record has remained relatively intact, the burial record to the east was interrupted in the late Early or Late Cretaceous. There is still controversy on the possible connection between the Songliao Basin and the eastern basin group, as well as the extent of the previously suggested “pan-Sanjiang” proto-basin that covered most current eastern satellite basins. To address these questions, we studied modern river catchments of variable sizes from sediment-supplying basement highs that cover most of eastern NE China to track the intense denudation events and provenance changes through the Cretaceous. We found a great inconsistency between detrital age patterns of the modern river sands and the spatial proportions of the source units. The detrital age distributions allow to re-evaluate the basement units that are mostly composed of the Triassic–Jurassic igneous suite and some metasedimentary units with Paleozoic ages, but the contribution from Proterozoic formations is negligible. Combining the newly dated modern catchment detrital U-Pb ages, the region-wide compilation of the basement emplacement ages, and the U-Pb ages in the Cretaceous formations in NE China, we systematically refine the Cretaceous provenance history. In the Early Cretaceous, the eastern satellite basins accumulated sediments from the Lesser Xing’an range (LXR) in the west, Zhangguangcai range (ZGCR) in the south, and the Nadanhada terrane (NT) in the east. The sediment of the southern Songliao Basin derived from the North China Craton (NCC) and from ZGCR but barely from the Great Xing’an Range (GXR). The Jiamusi uplift (JU) did not provide sediments to the eastern satellite basins and the Songliao Basin.In the Late Cretaceous, the eastern satellite basins received sediments from the exhumed JU but barely from ZGCR and LXR. The Songliao Basin still accumulated the sediments from the NCC, some south part of ZGCR, and GXR but minor from the central and northern ZGCR and LXR. Between the latest Early Cretaceous and early Late Cretaceous, a much wider proto-basin was suggested in NE China than previously assumed. It covered during its maximum extension period the current Songliao Basin, the eastern satellite basins, the LXR, ZGCR, JU, and partial GXR basement areas. The provenance change is induced by the Paleo-Pacific plate motion change, i.e., with the subduction direction change, the roll-back, and two-stage limited flat slab subduction trigged the formation of the basin-and-range system in NE China since the Early Cretaceous. Moreover, we suggest conducting modern sand provenance analysis to detect, verify or re-classify the ages of the zircon-bearing units, especially at a reconnaissance prospecting on areas covered by imprecise large-scale geological maps only to reveal the catchment better.

Petrography and geochemistry of Late Cretaceous clastic rocks in the northern Songliao Basin, north‐eastern China: Implications for provenance and weathering

Petrography and geochemical data of the Late Cretaceous clastic rocks in the northern Songliao Basin were used to infer the provenance and chemical weathering history. Petrographic data indicates that the composition of the sandstones is mainly quartz (34.48–50.43%), feldspars (14.16%–24.07%) and lithic fragments (33.91%–45.9%). Similar REE patterns and trace element ratios suggest that both the sandstones (LaCN/YbCN = 12.97; Eu/Eu* = 0.81; Th/Sc = 1.10) and mudstones (LaCN/YbCN = 10.02; Eu/Eu* = 0.71; Th/Sc = 1.10) were derived from a similar source area with felsic volcanic rocks as dominant contributors. The main provenance area is probably the eastern Zhangguangcai Range, the north‐eastern Lesser Xing'an Range and the south‐eastern Songliao Basin. A modelled mixture composed of 65% granodiorite in the Lesser Xing'an Range, 30% granite in Zhangguangcai Range and 5% epidiorite in the south‐eastern Songliao Basin, can be interpreted as the potential source compositions for the clastic sediments. This conclusion is reinforced by the major element of geochemistry composition and sandstone petrography. Relatively low Chemical Index of Alteration (CIA) values in mudstones (48.14–69.26) and sandstones (50.78–59.96) indicate mild chemical weathering conditions in the source area. The chemical weathering history suggests that a cold and arid climate prevailed during the Late Cretaceous.

We Write to You About Africa curated by Laura De Becker and Ozi Uduma; Wish You Were Here: African Art and Restitution curated by Laura De Becker, Bridget Grier, Timnet Gedar, and Ozi Uduma

We Write to You About Africa curated by Laura De Becker and Ozi Uduma; Wish You Were Here: African Art and Restitution curated by Laura De Becker, Bridget Grier, Timnet Gedar, and Ozi Uduma

Clay minerals study of the Kolosh Formation at selected sections from northern Iraq: Implications for provenance history

The mineralogical study using X-ray diffraction (XRD) supported by scanning electron microscopic (SEM) examination and energy-dispersive spectroscopy (EDS) on the claystone of the Kolosh Formation from northern Iraq was conducted to Shows the provenance history of rocks. Chlorite, montmorillonite, illite, palygorskite, and kaolinite were recorded in different amounts in the study area. The association of montmorillonite and chlorite in the claystone of the Kolosh Formation (Paleocene) refers to the marine environment. Chlorite and montmorillonite are the common minerals in the Kolosh Formation with less common of illite, kaolinite and palygorskite. These clay minerals are of authigenic, detrital and diagenetically origin, which are controlled mainly by the source rocks, paleoclimatic conditions and the burial diagenesis. The clay minerals assemblages refer to be derived mainly from Fe-Mg rich with minor Si-Al rich silicate minerals, which are very common in the ophiolites associated with the basic igneous rocks. These rocks composed the major lithological units in the Zagros Thrust Belt of NE Iraq.

Diffuse Reflectance Spectroscopy and Principal Component Analysis to Retrospectively Determine Production History of Plutonium Dioxide.

Diffuse reflectance spectroscopy measurements in the shortwave infrared (930-1600 nm) spectral region were acquired for Pu2(C2O4)3•9H2O and its thermal decomposition product, PuO2. We analyzed a total of eight PuO2 samples that were produced at different calcination temperatures (300, 350, 450, 525, 600, 675, 750, and 900°C). Our goal was to identify spectroscopic fingerprints that could be used to gain retrospective information regarding the production parameters of these important nuclear compounds. The diffuse reflectance spectrum of Pu2(C2O4)3•9H2O features several broad bands that currently preclude detailed analysis. However, all PuO2 samples produced relatively sharp spectral features that got sharper and more intense for samples that were produced at higher calcination temperatures. The electronic band observed at 1433 nm in the diffuse reflectance spectra of PuO2 was found to be a sensitive indicator of crystallinity; a result that is corroborated by ancillary Raman spectroscopy measurements. Principal component analysis of diffuse reflectance spectra was able to clearly rank and categorize PuO2 samples based on the calcination temperature that was employed during their production. Thus, we show herein that important retrospective information pertaining to the process history of PuO2 can be gained through the relatively simplistic combination of diffuse reflectance spectroscopy and principal component analysis. This discovery presents a new method for determining the provenance and process history of PuO2 and should have an impact in the fields of nuclear forensics and nuclear nonproliferation.

Myositis Autoantibodies in Patients with Suspected Post-Treatment Lyme Disease Syndrome.

Most patients suffering from Lyme disease are effectively treated with antibiotics. In some patients, however, problems persist for a long time despite appropriate therapy. The term post-treatment Lyme disease syndrome (PTLDS) is currently used for this condition in scientific literature. The pathogenesis is still not precisely known, but the involvement of immunopathological mechanisms is assumed. In our study, we analyzed the presence of autoantibodies including myositis-specific (MSA) and myositis-associated autoantibodies (MAA) in patients with laboratory proven history of Lyme disease and with clinical symptoms of PTLDS. A total of 59 patients meeting the criteria for PTLDS were enrolled in this study. The control group consisted of 40 patients undergoing differential diagnosis of neurological disorders without clinical and/or laboratory-proven history of Lyme disease. The presence of autoantibodies was determined by immunoblot methods and positive samples were further tested for serum creatine kinase (CK) and myoglobin levels. The presence of myositis autoantibodies was detected in 18 subjects with suspected PTLDS (30.5%), but only in 5% of control subjects exhibiting no evidence of Lyme disease history. The difference was statistically significant (p = 0.002). The subsequent biochemical analysis of muscle-damage markers in positive subjects found a mild elevation in six MSA/MAA-positive PTLDS patients. The study detected raised MSA/MAA autoantibodies formation in the group of PTLDS patients raising the question about their involvement in the pathogenesis of this syndrome.

The [Dissipative] Joy of Accounting: Desiring, Imagining, and Talking about NFTs While the Planet Burns

Promoters of art-oriented non-fungible tokens (NFTs) and online NFT auction houses like Foundation, OpenSea, and SuperRare claim to be “revolutionizing” and “democratizing” the worlds of art and art collecting by deploying blockchain technology to track sales and purchases and thereby eliminating the threats of transactional opacity and fraud that have long plagued art markets. This article complicates such claims by arguing that with NFTs, the usual clamoring for authenticity in art becomes so abstract that only code remains, as the aesthetic object becomes effectively indistinct from the account of its provenance and transaction history. An NFT, like a financial derivative, has no necessary or representational relationship with any actual underlying object. The artwork associated with the token is, rather, prised apart from its existence as property and finds its use not in being seen or felt, but in the truth of its copyability. Looking beyond the NFTs themselves, this article interrogates the discourses promulgating this trend. Comment threads in Reddit and Discord groups, for example, do much to channel the libidinal energy elicited by an artwork, while the digital token itself generates an acquisitive enthusiasm wholly apart from the sensation of seeing or hearing the associated work. Despite fronting radical social change, NFTs ultimately reinforce traditional forms of property and ownership, exhibit reactionary aesthetic and cultural values, and anticipate increasingly authoritarian modes of social control. Taking a wider view, I consider ours an age of post-information, wherein, contrary to Bateson’s classic definition of information in terms of a doubled difference, we find data-based artifacts like NFTs (following cryptocurrency) to be increasingly productive of widespread social and political indifference, a perpetuation of sameness, and an augmentation of the narcissistic ego. In this case: data as the afterlife of art.

Evolution of an Accretionary Complex (LeMay Group) and Terrane Translation in the Antarctic Peninsula

AbstractThe LeMay Group accretionary complex of Alexander Island (Antarctic Peninsula) comprises a 4 km thick succession of variably deformed turbidites associated with thrust slices of ocean floor basalts. The depositional age and provenance of the succession is uncertain with estimates ranging from Carboniferous to Cretaceous. The accretion history is also poorly established and whether the LeMay Group developed allochthonously and accreted during an episode of Cretaceous terrane translation. We have examined the geochronology and geochemistry of 22 samples from across the entire accretionary complex to determine its depositional, provenance and accretion history. The accretionary complex has been subdivided into four separate groups based on detrital zircon U‐Pb age and Lu‐Hf provenance analysis. Groups 1 and 2 are interpreted to be a continuation of the extensive Permian accretionary complexes of West Gondwana and have a depositional age of c. 255 Ma, with volcaniclastic input from the extensive silicic volcanism of the Choiyoi Province. Accretion of the LeMay Group to the continental margin developed during the mid‐Triassic, potentially related to the Peninsula Orogeny and an episode of flat‐slab subduction of the proto‐Pacific plate. Group 3 is only identified from an island to the west of Alexander Island and has a mid‐Cretaceous depositional age and provenance akin to offshore sequences from Thurston Island. A para‐autochthonous origin is suggested, with mid‐Cretaceous accretion associated with the melange belts of central Alexander Island. Group 4 is also a distinct unit with an Early Jurassic depositional age and a source more closely related to the Antarctic Peninsula.

Following Islamic teachings in the governance of Islamic society with an emphasis on transparency

Government is a rational necessity for mankind because a society without government leads to chaos. Government regulates the affairs of the individual and the community, implements the limits, and ensures the dignity and independence of the human society. Thinking in the main goals of the divine prophets, it is clear that achieving great goals such as liberating people from the domination and captivity of foreigners, comprehensive human education, reviving human values, establishing justice, bringing people to excellence and growth, etc., requires formation. In this context, transparency is a key attribute, which can bring much power to the expected level as an effective mechanism for protecting citizens’ political, economic, social and cultural rights. This concept is thus a valuable solution in the fight against sinister phenomena, such as political and economic corruptions, which are ranked as the biggest challenges facing any society. Even though transparency is nowadays deemed an emerging concept, its roots can be traced back to Islamic teachings, mainly during the short reign of Imam Ali, (peace be upon him [PBUH]). As a central figure, Imam Ali considered transparency as essential, and shared his views on governance and practical methods with the people and all audiences based on complete honesty and openness. As per Alavi thoughts, some criteria and indicators, both structural and behavioural, are involved in order to ensure the public good and meet the interests of all by providing the prerequisites for the realisation of transparency, at odds with the predominant ideas that transparency in governance, like many concepts in the political and governance-related spheres, has a western origin.Contribution: The concept of transparency, with a proven history in global experiences, accordingly holds a special place in the literature of Islamic governments. Given the importance of this topic, the present study with an emphasis on transparency aims to examine Islamic teachings on governance in Islamic societies, and reflect on governance transparency components in such societies, relying on expert opinions.

International agreements and entry points to adaptation and adoption of modern forest technologies, ecological restoration, climate change, ecotourism and agroforest enterprise based advanced materials of Sri Lanka and Regional Countries

Sri Lanka showed proven history of conserving richly endowed habitable natural capital that offers many eco-system services and essential launching pad for fast –track growth. This endowment could bring in desired economic and ecological improvements to living standards and for the well- being of future generations by sensibly put into sustainable use. Under the present development activities, country is facing major challenges of increasing severe climate change impacts, energy insecurity and ecological scarcity. Thus, present use of natural capital, carbon sequestration cyclic activities and forest enterprise are not fulfilling the conditions necessary for sustainability. International initiatives that have been taken and entry points for sustainable forest management and establishing of modern forest technologies are briefly discussed in this position paper to scope expansion of forestry enterprise development strategies. Thus, right policies for setting up right institutions of “Forest Development Authority” and “Forest Exchange System” and inter institutional set up of “Modern Forest Technology” for action in plan is justified.

Tracking the phage trends: A comprehensive review of applications in therapy and food production.

In the present scenario, the challenge of emerging antimicrobial resistance is affecting human health globally. The increasing incidences of multidrug-resistant infections have become harder to treat, causing high morbidity, and mortality, and are posing extensive financial loss. Limited discovery of new antibiotic molecules has further complicated the situation and has forced researchers to think and explore alternatives to antibiotics. This has led to the resurgence of the bacteriophages as an effective alternative as they have a proven history in the Eastern world where lytic bacteriophages have been used since their first implementation over a century ago. To help researchers and clinicians towards strengthening bacteriophages as a more effective, safe, and economical therapeutic alternative, the present review provides an elaborate narrative about the important aspects of bacteriophages. It abridges the prerequisite essential requirements of phage therapy, the role of phage biobank, and the details of immune responses reported while using bacteriophages in the clinical trials/compassionate grounds by examining the up-to-date case reports and their effects on the human gut microbiome. This review also discusses the potential of bacteriophages as a biocontrol agent against food-borne diseases in the food industry and aquaculture, in addition to clinical therapy. It finishes with a discussion of the major challenges, as well as phage therapy and phage-mediated biocontrols future prospects.

The Far-Field imprint of the late Paleozoic Ice Age, its demise, and the onset of a dust-house climate across the Eastern Shelf of the Midland Basin, Texas

The late Paleozoic is a period of pronounced climatic and tectonic change, characterized by the onset and disappearance of continental-scale glaciers across polar Gondwana, the formation of Pangea, and widespread large igneous province volcanism. The low-latitude equatorial tropics are assumed to be places of persistent warm and wet climatic conditions throughout the Phanerozoic, which through intense silicate weathering, exert a major influence on Earth’s climate via the consumption of atmospheric carbon through carbonic hydrolytic weathering, formation of clay minerals and deliverability of alkalinity to ocean basins. Here we investigate the late Paleozoic sedimentary record of the Eastern Shelf of the Midland Basin in order to refine the climatic and provenance record of this region. The Eastern Shelf of the Midland Basin was situated within the equatorial tropics throughout the late Paleozoic and was connected to the open ocean through a network of fluvial systems that drained into the marine Midland Basin. We present new U-Pb zircon geochronology (19 samples, 2591 analyses) and sedimentary petrography (11 samples, 5800 grain counts), which we integrate with previously published paleobotany, paleosol chemistry and clay mineralogy to provide a holistic climate and tectonic record from this region. We observe major changes in sedimentary processes that we attribute to the formation of Pangea, eustatic changes linked to a dynamic high-latitude glaciation and teleconnections with low latitude hydrology, and a long-term shift in the Earth climate system all of which result in a dynamic sediment provenance history. Late Pennsylvanian and earliest Permian deposits are enriched in zircons with local affinity and interpreted to reflect local uplift and repeat incision across the basin margin, the latter a result of glacioeustatic forcing during an “everwet” climate. A major paleoenvironmental shift occurs in the late early Permian, which is reflected by the transition from fluvial to mixed fluvial-aeolian and ultimately aeolian dominant sedimentation by the late Permian. The transition from fluvial to aeolian dominant sedimentation is accompanied by a change in clay chemistry, sedimentary rock textual maturity, paleosol morphology and a threefold increase in Paleozoic zircons in the mid to late Permian strata. Widespread loess deposits across equatorial Pangea during the Permian have been used to argue for the possibility of equatorial glaciers situated in highland settings during the early Permian. Conversely, our data suggest initiation of a substantial component of aeolian deposition across the field areas, which is coincident with widespread ice loss across high latitude Gondwana, and ultimately highlights the teleconnections between high latitude glaciation and the low latitude hydrologic cycle.

Audiological and vestibular symptoms following SARS-CoV-2 infection and COVID-19 vaccination in children aged 5–11 years

PurposeThe present study assessed the prevalence of audio-vestibular symptoms following SARS-COV-2 infection or COVID-19 vaccination among children, comparing the two groups. A further aim was to evaluate whether children with pre-existing unilateral hearing loss were more prone to adverse events. Materials and methodsThis retrospective study included children aged 5–11 years with normal hearing or a proven history of unilateral hearing loss who contracted SARS-CoV-2 or received two doses of COVID-19 vaccine. Tinnitus, hyperacusis, aural fullness, otalgia, otorrhea, new-onset hearing loss, vertigo and dizziness were investigated as possible complications of SARS-CoV-2 infection or the COVID-19 vaccine. ResultsThis study included 272 children (143 boys, 129 girls), with a mean age of 7.8 ± 2.3 years. Among these, 120 were affected by pre-existing unilateral hearing loss.The most common audio-vestibular symptoms reported by children following SARS-CoV-2 infection and COVID-19 vaccination were aural fullness (33/132, 25 %) and dizziness (5/140, 3.6 %), respectively. All symptoms following COVID-19 vaccination resolved within 24 h.Compared to children who received the COVID-19 vaccine, those infected with SARS-CoV-2 had a higher prevalence of tinnitus (p = 0.009), hyperacusis (p = 0.003), aural fullness (p < 0.001), otalgia (p < 0.001), otorrhea (p < 0.001), and vertigo (p = 0.006). Two girls also experienced new-onset unilateral sensorineural hearing loss following SARS-CoV-2 infection.Children with a known history of unilateral hearing loss did not have a higher prevalence of audio-vestibular symptoms than children with normal hearing. ConclusionsOur results suggest that the COVID-19 vaccine is safe and can be recommended for children with unilateral hearing loss without fear of possible audio-vestibular sequelae.

Lost, Burned and Recovered: Tracing the Provenance History of a Copy of Caxton’s Golden Legend in the John Rylands Library

Abstract On 29 June 1865, a fire at Sotheby’s in London destroyed extensive libraries prior to their sales. Among them was a copy of William Caxton’s Golden Legend owned by George Offor. This article demonstrates that this copy, hitherto believed to be lost, managed to survive the fire and now is Incunable Collection R4591 in the John Rylands Library at the University of Manchester. Further evidence confirms that before it was put on sale, Offor used it as ‘a hospital copy’ in order to ‘sophisticate’ other copies of the Golden Legend. This article also argues that prior to Offor’s ownership the copy was in the hands of a publisher and bookseller William Pickering, and he too removed several leaves from it and repurposed them. By filling the gap in the provenance history of this copy, this article throws further light on the antiquarian book trade in the nineteenth century.