Abstract State-of-the-art data sources, such as Doppler radar, automated surface observations, wind profiler, digital satellite, and aircraft reports, are for the first time providing the capability to generate real-time, operational three-dimensional gridded datasets with sufficient spatial and temporal resolutions to diagnose the structure and evolution of mesoscale systems. A prototype data assimilation system of this type, called the Local Analysis and Prediction System (LAPS), is being developed at the National Oceanic and Atmospheric Administration's Forecast Systems Laboratory. The investigation uses the three-dimensional LAPS analyses for initialization of the nonhydrostatic Regional Atmospheric Modeling System (RAMS) model developed at Colorado State University to create a system capable of generating operational mesoscale predictions. The LAPS/RAMS system structured for operational use can add significant value to existing operational model output and provide an improved scientific understanding...