In search for the mechanism of insecticidal action of nicotinoids, the kinetics of house fly head cholinesterase inhibition by nicotine were studied to determine the type of in-hibition. The pH dependency of inhibition was interpreted in terms of protonation of nitrogen atom in the molecule and the inhibition was shown to be the mixed type closing to competitive type. The Michaelis constants are 3.5×10-4M and 4.1×10-4M, while the apparent inhibition constants obtained are 1.0×10-3M and 2.3×10-3M at pH 7.4 and 8.4, respectively. The type of the inhibition by nicotine monomethiodide earring univalent cation was competitive and the apparent inhibition constant is 1.5×10-4M. These data indicated that the cationic head of nicotinium ion interacts with the anionic site in the active center of cholinesterase.