Induction Of Protocorm-like Bodies Research Articles

Suitable Conditions for the Induction and Micropropagation of PLBs in Some Monopodial Orchids.

Liquid media suitable for the induction and micropropagation of protocorm-like bodies (PLBs) in Vandofinetia Nara ‘Yumika Pink’ were developed by modifying MS basal medium. The best medium obtained for PLBs induction was one-fourth strength MS medium containing 30g/l sucrose and for PLBs micropropagation was one-fourth strength MS medium containing 10g/l sucrose in which FeEDTA was replaced by 6.95mg/l FeSO47H2O. Usefulness of the latter medium was confirmed in other monopodial orchids such as Renanetia, Darwinara, Ascocentrum and Ascofinetia. After transfer onto 2g/l Hyponex (N:P:K=10:30:20) medium containing 2g/l proteose peptone and 20g/l sucrose for 2 months, the PLBs (both those regenerating and those not regenerating plantlets) were subcultured into MS medium containing 30g/l sucrose at 2 months intervals for 4 months. More than 10, 000 plantlets ready for transplanting to pots were obtained efficiently from one floral bud within a year for each orchid cultivar. These results suggest that the methodology could be used on a commercial scale for micropropagation of a wide range of monopodial orchids.

フウラン（Ｎｅｏｆｉｎｅｔｉａ ｆａｌｃａｔａ Ｈｕ）の小花からのプロトコーム様球体の誘導と植物体の再生

Florets of Neofinetia falcata Hu were cultured on modified MS medium containing 412.5mg/l NH4NO3 and 950mg/l KNO3. A combination of 1μM BA and 10μM NAA was the most effective for the induction of protocorm-like bodies (PLB). Plantlet formation from PLB was enhanced on modified MS medium supplemented with 5g/l sucrose.

In Vitro Propagation of Aranda Wendy Scott and Aranthera James Storei1

Abstract Clonal propagation in vitro of Aranda Wendy Scott ‘Greenfield’ and Aranthera James Storei was achieved with Vacin and Went medium (VW) supplemented with coconut water (CW) (15% by volume) or green banana (GB) (50 g/liter) or by omission of sucrose (Su). Induction of protocorm-like bodies (plb) on explants of apical and axillary buds was accomplished on VW + 15%CW and multiplication of plb in VW − 2%Su + 15%CW. Shoots were induced on solid VW − 2%Su for Aranthera James Storei and solid VW − 2%Su + 15%CW + 5%GB for Aranda Wendy Scott. Roots were induced on solid VW + 15%CW + 5%GB medium.