IN the course of a study of some chemically unstable constituents of muscle tissue, we have made successful use of the following method for obtaining protein-free extracts of muscle. It has the merit of yielding, without departure from neutrality, a protein-free solution of the water-soluble constituents of muscle in a concentrated form. We have been unable to find a description of this technique in the literature, but should be grateful for information as to earlier examples of its use.