Protein-bound Iodine Research Articles

Seasonal dynamics of indicators of mineral metabolism in horses in the conditions of the Leningrad region

The article analyzes the seasonal dynamics of mineral metabolism indicators in adult horses kept in private stables in the Leningrad region. As a result of the research, knowledge of the concentration and dependence of indicators on the seasonality of the year was obtained. It is important to remember that macro- and microelements are not synthesized in the body, they come from food, water, and air. The degree of their assimilation depends on the state of the respiratory and digestive organs. The most difficult period for animals is winter, when there is a decrease in the resistance of the body as a whole due to poor climatic conditions and minimizing physical activity in the fresh air. All these are predisposing factors for the imbalance of indicators at the level of the whole organism.It is established that in the autumn-winter period there is a decrease in indicators relative to the average annual digital values. Thus, there is a linear relationship between the concentration of calcium and phosphorus in blood serum – the minimum values are observed in winter and are 2.53±0.27 mmol/l and 1.76± 0.11 mmol/l, respectively. And the maximum values are typical for the autumn period and are 3.63±0.29 mmol/l for calcium and 2.33±0.12 mmol/l for phosphorus. Similar dynamics is observed in other indicators of the body's mineral metabolism. Thus, for trace elements such as copper, iron, protein-bound iodine, the minimum values were noted in the spring period. This can be explained by a decrease in the mineral components of the feed itself, as a result of long-term storage, resulting in changes in the chemical composition of vegetable feed with fat oxidation. Microelementoses and osteodystrophic processes are observed in animals. The autumn period is characterized by the highest concentrations of minerals in the blood serum of horses, on average by 8-12% compared with the average annual figures, This is explained by the fact that it was in the summer period that the greatest benefit was obtained from juicy plant substances of feed and proper exercise.This knowledge is necessary to understand the processes occurring in the body of horses at different times of the year and the possibility of correcting mineral supplements based on the data obtained

Assessment of Hepatoprotective and Antioxidant Effect of Acioa barteri Extract (ABE) in Alloxan-Induced Diabetic Rats

This study aimed to investigate the effects of Acioa barteri extract (ABE) on hepatocellular enzyme activity, hepatic function, and antioxidant stress indices in diabetic rats induced with alloxan. The antidiabetic effect of ABE was evaluated in six experimental groups: normal controls, diabetics untreated, diabetics treated with 200mg/kg, 400mg/kg, or 800 mg/kg ABE, and diabetics treated with 3 mg/kg Glibenclamide. ABE was orally administered to induce diabetes, and alloxan-monohydrate was intraperitoneally administered. Diabetic untreated rats exhibited significantly elevated levels of alkaline phosphatase, aspartate, and alanine transaminase activities, as well as higher concentrations of total bilirubin, conjugated bilirubin, and malondialdehyde. They also showed decreased levels of total protein, albumin, globulin, and protein-bound iodine, along with reduced antioxidant enzyme activity. In contrast, diabetic rats administered ABE demonstrated reduced hepatocellular enzyme activity and improved hepatic function. These rats exhibited increased levels of total protein, globulin, and albumin, as well as higher levels of glutathione, superoxide dismutase, glutathione peroxidase, and catalase activities, compared to diabetic untreated rats. The findings suggest that ABE may help prevent oxidative stress and improve hepatic functions in diabetic rats. ABE treatment led to decreased hepatocellular enzyme activity and improved hepatic function, along with increased antioxidant enzyme activities. These results highlight the potential of ABE as a therapeutic option for diabetes-induced liver dysfunction. Further research is warranted to explore its mechanisms of action and potential clinical applications.

The Presence of Anti-TPO Antibodies Increase the Likelihood of Post-I131 Hypothyroidism.

The use of radioactive iodine in the treatment of hyperthyroidism is common practice. However, a standardized treatment protocol with regard to radioactive iodine treatment (RAI) remains subject to discussion. We retrospectively analyzed 100 patient records. Patient diagnosis, age, gender, body mass index (BMI), dose of radioactive iodine, thyroid size, the 24 h radioiodine uptake (24 h RAIU) and protein bound iodine (PBI) were deducted, as well as the use of antithyroid drugs prior to RAI. Biochemical parameters were obtained, such as TSH, fT4, fT3, Anti-TPO, Anti-TG antibodies and thyroid stimulating antibodies. After 5 years of follow-up, 46% of the patients proved to be hypothyroid, whereas 8% of the patients were not cured after one dose of RAI. One year after RAI, a larger proportion of patients with a toxic nodule developed hypothyroidism compared to patients with a multinodular goiter (MNG) (44.2% vs. 21.2%). Radioactive iodine dose, PBI, RAIU, BMI, size of the thyroid gland, diagnosis, age and TPO-antibodies showed statistically significant differences in the development of hypothyroidism. Furthermore, thiamazole pretherapy was shown to be a predictor of hypothyroidism, as well as a high PBI value, exhibiting a positive predictive value of 85.2% when the PBI exceeded 0.16. We suggest a standardized measurement of TPO-Ab's to further determine their role in the development of hypothyroidism after RAI. The empirical dosing regimen was very effective, illustrating a 92% cure rate after 1 dose.

Influence of a lick-briquette on the mine-ral composition and guality of cows milk

Numerous experiments have shown that the organism of animals should receive in optimal proportions along with feed a sufficient amount of minerals, which determines their physiological status. Minerals together with feed additives in the process of absorption enter the milk. On average, a productive cow's milk contains up to 1% of minerals. Dagestan Republic, due to natural, climatic and zonal features, is experiencing a shortage of minerals in the soil and pasture vegetation. In this regard, their content in feed is insufficient to maintain the optimal level of the animal organism. Long-term shortage leads to a breakdown of metabolic processes, which leads to the development of diseases associated with their deficiency. Recently, to compensate the lack of minerals, feed additives in the form of mineral licks - briquettes are widely used. The paper presents data, obtained as a result of a scientific and economic experiment in the Tarumovsky district of Dagestan Republic. The experimental group of dairy cows, along with the main diet, additionally received a lick - briquette, in dose 4 kg. According to the results of the researches, a beneficial effect of a lick - briquette on the mineral composition of the milk of the experimental group of cows was established, with an increase in potassium, sodium, magnesium, calcium and phosphorus, respectively, on 2.94; 100.0; 46.15; 11, 40; 52.45%; iron, manganese, zinc, copper, cobalt, selenium and iodine (protein-bound iodine) - on 60.0; 107.14; 118.18; 21.31; 98.27;131.1; 179.50%, compared with control. Consequently, with an increase of the level of minerals, the qualitative composition and technological properties of milk improve as a valuable product for human nutrition.

Adjustment of the iodine ICRP population pharmacokinetic model for the use in thyroid cancer patients after thyroidectomy

Biokinetic models developed for healthy humans are not appropriate to describe biokinetics in thyroid cancer patients following thyroidectomy. The aim of this study was to adjust the population model for iodine proposed by the International Commission on Radiological Protection (ICRP) for the use in these patients. Rate constants of the ICRP publication 128 model for iodine were adjusted using the population modelling software package Monolix to describe activity retention in whole-body, thyroid, blood and protein-bound iodine observed in 23 patients. The new set of rate constants was compared to the four uptake scenarios proposed in ICRP publication 128. Flow from the inorganic iodide in blood compartment into the first thyroid compartment decreases to 0.15 d−1 compared to a value of 7.27 d−1 for the ICRP publication 128 model with a medium uptake. The transfer from first to second thyroid compartments and the outflow from the second thyroid compartment increases. An increased turnover rate of extrathyroidal organic iodine is observed. The rate constant from inorganic iodide in blood to kidney was also adjusted. Overall a good agreement was found between the adjusted model and the activity retention in thyroid cancer patients. The adjustment of population pharmacokinetic models to describe the biokinetic properties of specific patient populations for therapeutic radiopharmaceuticals is essential to capture the changes in biokinetics. The proposed set of rate constants for the established ICRP publication 128 model can be used to more accurately assess radiation protection requirements for the treatment of thyroid cancer patients using radioiodine.

Clinical symptoms of iodine and zinc deficiency in dairy cows

Numerous clinical observations indicate that the silage-concentrate type of dairy cows feeding causes a cascade of pathobiochemical processes occurring by the type of metabolic acidosis, ketosis, hepatosis, with a violation of protein, fat and carbohydrate metabolism. Animals cannot realize the genetically inherent productivity potential.The culling of cows from the main herd is more than 37%. The main reasons for culling cows are hypo - and agalactia as a result of mastitis and ketosis, as well as infertility against the background of repeated infertile inseminations. Allotriophagy, unsteadiness of teeth, resorption of the last tail vertebrae, curvature of the vertebral column, traces of rib fractures, difficulty in lifting and moving when the content of total calcium and inorganic phosphorus in the blood is at the lower limit of physiological values indicate osteodystrophy. Exo-or endophthalmos, myxedema, forelock and mane, disheveled and curly hair, abortions, the birth of premature, ugly, stillborn, sparsely haired or “naked” calves against the background of low levels of protein bound iodine (PBI) in the blood indicate iodine deficiency in their body (hypomicroelementosis J). Coarsening and increased folding of the skin in the area of the head, neck and withers, active keratization on the skin in the area of articular surfaces and croup, thickening of the corolla zone and the border of the hooves in the form of a roller, ski-like growth and deformation of the hoof horn, enlargement and deformation of the joints, decreased reproductive function and low levels of zinc in the blood indicate parakeratosis of cattle (zinc deficiency).

Implications of extrapolative factors and their role in the development of breast cancer in females susceptible to Autoimmune Thyroid Disorders (AITDs)

Background: Apart from different cancer types in females, breast cancer falls among frequent cancers all over the world. The data from the current studies shows that females with advanced stages of breast cancer also prone to Autoimmune Thyroid Disorders (AITDs). Methodology: Hundred breast cancer patients (Stage-III) with palpable thyroid nodule in the age group of 30-75 y were eligible for inclusion in the study. Hundred age and sex-matched clinically apparently healthy individuals were included as controls. FT3, FT4, TSH, TPOAb, TgAb, TSHr Ab, prolactin and estradiol were estimated by using ELISA kit method. Serum Protein-Bound Iodine (PBI) and selenium were estimated by using a flow injection hydride generation Atomic Absorption Spectrometric (AAS) method. Results: The mean age and weight for diseased group were 58.36 ± 11.78 y and 74.96 ± 12.66 kg. The decreased levels (3.73 ± 0.189 pmol/L) of T3 were observed in newly diagnosed breast cancer patients as compared to controls (4.72 ± 0.24 pmol/L). Higher levels of T4 (17.86 ± 4.98 pmol/L) were recorded in patients as compared to controls (10.11 ± 1.64 pmol/L). The serum TSH levels in the breast cancer patients were raised (3.54 ± 0.26 mIU/L) in contrary to control group (1.9 ± 0.134 mIU/L). Significantly higher levels of Thyroglobulin Antibodies (TgAb), Thyro-Peroxidase Antibodies (TPOAb) and Thyrotropin Stimulating Hormone receptor Antibodies (TSHrAb) were recorded in breast cancer patients (62.07 ± 6.94 pmol/L, 24.1 ± 6.81 and 3.1 ± 0.43 IU/L pmol/L) as compared to controls respectively. The levels of iodine and selenium in patients were reduced (32.03 ± 4.76 ng/ml and 51.03 ± 2.13 mlU/L) as compared to the controls. Highly significant higher serum levels of prolactin (35.09 ± 4.76) were observed in diseases group as compared to controls (7.14 ± 1.57 pg/ml). Serum estrogen levels in patients were 10.47 ± 1.67 pg/ml, while in healthy individuals they were 6.55 ± 0.88 pg/ml, which shows statistically highly significant profile between the two groups. Conclusion: The present study show an association in between breast cancer and thyroid disorders, as thyroid hormone profile regulates breast cancer initiation. Therefore, these biomarkers can be used in prognosis i.e., in management and progression of breast cancer.

TAZ Induction Directs Differentiation of Thyroid Follicular Cells from Human Embryonic Stem Cells.

The differentiation program for human thyroid follicular cells (TFCs) relies on the interplay between sequence-specific transcription factors and transcriptional co-regulators. Transcriptional co-activator with PDZ-binding motif (TAZ) is a co-activator that regulates several transcription factors, including PAX8 and NKX2-1, which play a central role in thyroid-specific gene transcription. TAZ and PAX8/NKX2-1 are co-expressed in the nuclei of thyroid cells, and TAZ interacts directly with both PAX8 and NKX2-1, leading to their enhanced transcriptional activity on the thyroglobulin (TG) promoter and additional genes. The use of a small molecule, ethacridine, recently identified as a TAZ activator, in the differentiation of thyroid cells from human embryonic stem (hES) cells was studied. First, endodermal cells were derived from hES cells using Activin A, followed by induction of differentiation into thyroid cells directed by ethacridine and thyrotropin (TSH). The expression of TAZ was increased in the Activin A-derived endodermal cells by ethacridine in a dose-dependent manner and followed by increases in PAX8 and NKX2-1 when assessed by both quantitative polymerase chain reaction and immunostaining. Following further differentiation with the combination of ethacridine and TSH, the thyroid-specific genes TG, TPO, TSHR, and NIS were all induced in the differentiated hES cells. When these cells were cultured with extracellular matrix-coated dishes, thyroid follicle formation and abundant TG protein expression were observed. Furthermore, such hES cell-derived thyroid follicles showed a marked TSH-induced and dose-dependent increase in radioiodine uptake and protein-bound iodine accumulation. These data show that fully functional human thyroid cells can be derived from hES cells using ethacridine, a TAZ activator, which induces thyroid-specific gene expression and promotes thyroid cell differentiation from the hES cells. These studies again demonstrate the importance of transcriptional regulation in thyroid cell development. This approach also yields functional human thyrocytes, without any gene transfection or complex culture conditions, by directly manipulating the transcriptional machinery without interfering with intermediate signaling events.

Diselementosis as a risk factor of embryo loss in lactating cows

The aim of this research was to reveal the impact of bioelemental status of dairy cows on intrauterine growth restriction and embryo death at early gestation stages. The research included 22 Holstein cows with average annual dairy productivity of 6400-7600 kg. All animals were subjected to transrectal ultrasonography to determine corpus luteum and pregnancy status, and their blood was sampled for biochemical analyses on the 19th-22nd, 28th-32nd, and 38th-45th days of gestation. Zinc, copper, manganese, and selenium blood contents were determined by atomic absorption spectrophotometry. Serum levels of calcium, phosphorus, and magnesium were determined with an automatic biochemistry analyzer. Protein-bound iodine was determined spectrophotometrically. Analysis of results was realized based on the character of embryo development: physiological formation (Group I, n = 9), growth restriction (Group II, n = 8), and death (Group III, n = 5). Serum concentrations of Ca, P, and Mg were 2.76-2.84 mmol/L, 1.77-1.92 mmol/L, and 1.13-1.36 mmol/L in range; blood contents of Zn, Cu, Mn, and Se were 40.8-52.7 µmol/L, 15.9-18.7 µmol/L, 2.50-3.43 µmol/L, and 1.23-1.41 µmol/L in range; and protein-bound iodine was 0.32-0.36 µmol/L in Group I. Serum concentration of the studied trace elements was lower in Groups II and III in comparison with Group I. Cows of Group II demonstrated the following indices: calcium content, 2.72-2.79 mmol/L; phosphorus content, 1.79-1.97 mmol/L; magnesium, 0.98-1.09 mmol/L; zinc, 33.8-36.8 µmol/L; copper, 13.9-15.3 µmol/L; manganese, 2.50-2.89 µmol/L; selenium, 1.04-1.11 µmol/L; protein-bound iodine, 0.29-0.37 µmol/L. The difference according to the level of serum Ca reached 4.2%, P 6.8%, and Mg 23.5% (P < 0.01), while according to blood concentration Zn was 30.2% (P < 0.001), Cu 21.6%, Mn 15.2%, Se 26.2% (P < 0.05), and protein-bound iodine 21.3% (P < 0.01). Cows of Group III demonstrated the following indices: calcium content, 2.48-2.56 mmol/L; phosphorus content, 1.74-1.93 mmol/L; magnesium, 0.99-1.02 mmol/L; zinc, 31.2-34.0 µmol/L; copper, 12.2-15.4 µmol/L; manganese, 2.24-3.06 µmol/L; selenium, 1.00-1.03 µmol/L; protein-bound iodine, 0.24-0.30 µmol/L. The differences in comparison with cows of Group I reached 11.4% (P < 0.05), 9.4%, 27.2% (P < 0.001), 35.5% (P < 0.001), 20.6% (P < 0.05), 15.2% (P < 0.05), 29.1% (P < 0.05), and 33.3% (P < 0.001), respectively. Disorders of trace elements in cows are one of the main reasons for intrauterine growth restriction and embryo death. Primarily it is connected with magnesium, zinc, copper, selenium, and iodine deficits that have direct or indirect effects on growth, generation, and differentiation of the cells of the developing embryo. Lactating cows with a high level of milk production demonstrate high risk of diselementosis and consequently embryopathy development. Correction of diselementosis in lactating cows should be considered as one of the ways for solving the problem of their fertility.

The History and Future of Treatment of Hypothyroidism.

Thyroid hormone replacement has been used for more than a century to treat hypothyroidism. Natural thyroid preparations (thyroid extract, desiccated thyroid, or thyroglobulin), which contain both thyroxine (T4) and triiodothyronine (T3), were the first pharmacologic treatments available and dominated the market for the better part of the 20th century. Dosages were adjusted to resolve symptoms and to normalize the basal metabolic rate and/or serum protein-bound iodine level, but thyrotoxic adverse effects were not uncommon. Two major developments in the 1970s led to a transition in clinical practice: 1) The development of the serum thyroid-stimulating hormone (TSH) radioimmunoassay led to the discovery that many patients were overtreated, resulting in a dramatic reduction in thyroid hormone replacement dosage, and 2) the identification of peripheral deiodinase-mediated T4-to-T3 conversion provided a physiologic means to justify l-thyroxine monotherapy, obviating concerns about inconsistencies with desiccated thyroid. Thereafter, l-thyroxine monotherapy at doses to normalize the serum TSH became the standard of care. Since then, a subgroup of thyroid hormone-treated patients with residual symptoms of hypothyroidism despite normalization of the serum TSH has been identified. This has brought into question the inability of l-thyroxine monotherapy to universally normalize serum T3 levels. New research suggests mechanisms for the inadequacies of l-thyroxine monotherapy and highlights the possible role for personalized medicine based on deiodinase polymorphisms. Understanding the historical events that affected clinical practice trends provides invaluable insight into formulation of an approach to help all patients achieve clinical and biochemical euthyroidism.

Goitrogenic Effects Of Mustard Seed (Bassica Junca) Oils

The goitrogenic effects of commercial mustard seed aws studied when the oil was fed to rabbits.A toatl of 9 rabbits divided into 3 groups of 3 rabbits each was used in the study.Those in group A were given 5ml of the mustard oil per kg body weight 3 times daily,those in group B were given 10ml per kg body weight 3 times daily while those in group C were not given the oil at all and served as control.The mustard seed oil was fed in a total of 4 days.The mean values of the protein-bound iodine of groupA rabbit were 0.25ug/dl+/-0.13(an increase of 45%),0.58ug/dl+/-0.24(an increase of 61.10%) and 1.00ug/dl +/-0.33(an increase of 72.24%) from day 1 to day 4 respectively.In group B animals,the protein-bound iodine levels were 0.23ug/dl+/-0.32(an increase of 74.55%),1.78ug/dl+/-0.32(an increase of 85%) and 3.51 ug/dl +/- 0.47(an increase of 95%) from day 1 to day 4 respectively.The levels seen in group A and B showed dose-dependence.In both groups,significant daily increase in protein bound-iodine were observed and ranged from 45-72.24% in group A in 4 days and 74.55- 95% in group B for the same period.Group C animals had fairly constant protein-bound iodine levels within the study period because they did not receive the mustard seed oil at all. Selective phytochemical anlysis revealed the presence of many known goitrogens-flavonoids,phenolic compounds,tannins,alkaloids but not anthraquinones.This could explain the elevation of protein-bound iodine levels in these animals within a few days.

Effects Of Mustard Seed Oil On Prolong On Protein-Bound Iodine,Globulin And Albumin Of Albino Rats

The effect of commercial-grade mustard seed oil on plasma protein-bound iodine (PBI), globulin and albumin levels was studied in albino rats. Group 2 (experimental rats) was given 0.1ml of the oil orally three times daily for five days, while group 1 (control) was not. At the end of the five days, the mean concentrations of PBI, globulin and albumin levels in the experimental rats were 3.96µg/ml, 0.35g/dl and 3.85mg/100ml respectively when compared with the control values of 3.43µg/ml. 0.35g/dl and 2.55mg/100ml respectively. This study indicates that mustard seed oil causes the binding away of metabolic iodine that does not involve immunologic mechanisms since the globulin level did not differ from that of the control, while not impairing the transport of thyroxine to the target organs as albumin level was not lowered. Keywords: Mustard seed oil, thyroxine, albumin and iodine.

A dose-effect correlation for radioiodine ablation in differentiated thyroid cancer

The aim of this study was to determine the range of absorbed doses delivered to thyroid remnants, blood, and red marrow from fixed administrations of radioiodine and to ascertain whether the success of ablation is more dependent on these absorbed doses than on the administered activity. Twenty-three patients received 3,000 MBq radioiodine following near-total thyroidectomy. The maximum absorbed dose to remnants was calculated from subsequent single photon emission tomography scans. Absorbed doses delivered to blood and red marrow were calculated from blood samples and from whole-body retention measurements. The protein bound iodine (PBI) was also calculated. Maximum absorbed doses to thyroid remnants ranged from 7 to 570 Gy. Eighteen of the 23 patients had a successful ablation. A significant difference was seen between the absorbed doses delivered to thyroid remnants, blood, and red marrow for those patients that had a successful ablation compared to those with a failed ablation (p = 0.030, p = 0.043 and p = 0.048, respectively). The difference between the PBI values acquired at day 1 and day 6 were also indicative of response (p = 0.074). A successful ablation is strongly dependent on the absorbed dose to the thyroid remnant. Dosimetry-based personalized treatment can prevent both sub-optimal administrations, which entails further radioiodine therapy, and excessive administration of radioactivity, which increases the potential for radiation toxicity.

Iodine and Magnesium Levels in Maternal and Umbilical Cord Blood of Preeclamptic and Normal Pregnant Women

The objective of this study was to investigate the relationship between preeclampsia and iodine levels and magnesium concentration in the blood of subjects in the northeast Anatolia region where iodine deficiency is common. Blood specimens were obtained from 24 preeclamptic and 16 healthy pregnant women. Iodine levels in blood were determined by the Foss method based on the Sandell-Kolthoff reaction. Serum protein-bound iodine (PBI) levels and magnesium concentration in maternal blood were lower in patients with severe preeclampsia compared to normal pregnant women (8.46 +/- 1.22 vs. 11.46 +/- 1.71 microg/dL, p < 0.001, 1.63 +/- 0.05 vs. 1.86 +/- 0.05 mg/dL, p < 0.001, respectively). Serum PBI levels and magnesium concentration in umbilical cord blood were higher in patients with severe preeclampsia than in normal pregnant women (8.84 +/- 1.9 vs. 7.33 +/- 1.07 microg/dL, p < 0.05, 2.48 +/- 0.03 vs. 2.02 +/- 0.01 mg/dL, p < 0.001, respectively). There was a positive correlation between the serum PBI levels in maternal blood and magnesium concentration in maternal blood in patients with severe preeclampsia (r = 0.41, p < 0.05). Thus, iodine may be one factor contributing to the pathophysiology of preeclampsia. Iodine supplementation may be effective therapy in preeclamptic in pregnant women.

Fractionation analysis of iodine in bovine milk by preconcentration neutron activation analysis

Iodine is an essential trace element for human beings. The main source of iodine is generally food items such as fish and milk. Either the lack or the excess of iodine can cause health problems. There exists an increasing interest in the determination of total iodine as well as various species of iodine in milk. We have developed an epithermal neutron activation analysis method with a Compton suppression (ENAA–CS) counting system for the determination of ng mL −1 levels of iodine. We have also employed chemical separation methods prior to ENAA–CS to measure the fraction-specific concentrations of iodine in bovine milk. We have measured the following iodine concentrations in homogenized milk (3.25%milk fat): 0.48 ± 0.02 μg mL −1 of total iodine, 0.020 ± 0.003 μg mL −1 of lipid-bound iodine, 0.039 ± 0.002, 0.019 ± 0.002 and 0.021 ± 0.004 μg mL −1 of protein-bound iodine depending on the protein separation method and 0.45 ± 0.02 μg mL −1 of inorganic species.

Human Serum Protein-Bound iodine and Protein Fractions at Different Physiological and Narcotic States

Iodine profile of Nigerians at different ages in both sexes and in pregnant women, and under narcotic influence, such as alcoholism, cigarette smoking and marijuana addiction were studied. Their serum total protein, albumin and globulin concentrations were also determined. Results of the study showed that serum protein bound iodine levels increased significantly with age in both sexes and in pregnant women, while it decreased significantly in alcoholics, cigarette smokers and marijuana addicts. Serum total protein, albumin and globulin levels increased with age in both sexes and in pregnant women, but were decreased significantly in alcoholics, cigarette smokers and marijuana addicts. Data of the study indicate that serum protein bound iodine levels were potentiated by age, sex and pregnancy, but were impaired by alcoholism, cigarette smoking and marijuana addiction. In addition, serum total protein, albumin and globulin levels were affected by age, sex, pregnancy, cigarette smoking and marijuana addiction. Alcoholism, cigarette smoking and marijuana addiction may have deleterious effects on thyroid function and protein synthesis. NQJHM Vol. 14 (3&4) 2004: pp. 257-261

Blood and Urine Iodine Levels in Patients with Gastric Cancer

In this study, we aimed to investigate whether there is any relationship between gastric cancer and iodine concentrations in blood and urine in the northeast Anatolia region, where iodine deficiency is common. A total of 56 patients, diagnosed as gastric cancer and 25 healthy volunteers were included in the study. The methods used were based on the Sandell-Kolthoff reaction. The urine iodine concentration (UIC) and serum protein-bound iodine (PBI) levels were higher in patients with gastric cancer compared with healthy control subjects. The UIC in stage IV was higher than all other stages and the control group. The UIC was higher in stages III and IV compared with stages I and II. However, serum PBI levels in stage III were higher compared with stages I and II and also control group. The serum PBI level in stage IV was higher than stage II and the control group. In the patient and control groups, there were no significant differences in serum PBI and UIC with regard to age or sex. Our results suggested that urinary and blood iodine concentration might be a useful marker for following the disease.

Value of protein-bound radioactive iodine measurements in the management of differentiated thyroid cancer treated with (131)I.

Measurement of the protein-bound radioactive iodine level (PBI(131)) in the plasma of patients following (131)I-iodide administration for thyroid cancer has been re-examined in a retrospective study of 171 patient episodes. It is shown that whereas the previously used threshold value for the measurement at 6 days does not correlate well with the 3-day whole body scan, there is good agreement between the scan and the temporal changes in PBI(131) from 1-6 days: an increasing PBI(131) correlates with a positive scan, and a decreasing PBI(131) with a negative scan. The area under the curve (AUC) for the PBI(131)-time curve is related to the absorbed dose for the tumour. For a small group of 11 patients, dosimetry estimates were made from serial scans, quantified with phantoms; these absorbed doses correlated with the AUC and the 6-day PBI(131). Therefore, it is suggested that these parameters may be useful in predicting absorbed radiation dose in these patients.

Age-dependent thyroid absorbed doses for radiobiologically significant radioisotopes of iodine.

In light of the post-Chernobyl increase in pediatric thyroid cancer incidence, among other recent events, there is renewed interest in radioiodine thyroid dosimetry and effects. Among the radioiodines produced in fission of 235U, only 131I [(T1/2)p = 8.04 d], 132I (2.3 h), 133I (20.3 h), and 135I (6.7 h) may undergo significant environmental dispersion. Age-dependent thyroid absorbed dose estimates for these radiobiologically significant radioiodines and for the "medical" radioisotopes 123I (13.2 h) and 125I (60 d) have been derived, incorporating the effect of absorption following inhalation or ingestion. This effect has generally been ignored in previously derived estimates of radioiodine absorbed doses to the thyroid. Based on the latest ICRP lung and gut models, inhaled radioiodine is absorbed at a rate of 0.175 h(-1) and exhaled at 0.101 to 0.118 h(-1) (depending on age) and ingested radioiodine is completely absorbed in the stomach at a rate of 1 h(-1). Whole-body compartmental models (SAAM II) were fit to previously published 24-h thyroid uptakes, thyroid half-times, and 48-h plasma concentration of protein-bound iodine. The resulting fitted models were used to calculate thyroid residence times of radioiodine. The mean thyroid absorbed doses [cGy/37 kBq (rad/microCi) injected intravenously] were then calculated using the age-dependent S(thyroid<--thyroid) factors (MIRDOSE III), with the highest doses (from 0.49 for 123I to 36 for 131I) in newborns and the lowest doses (from 0.014 for 123I to 1.4 for 131I) in adults in inverse relation to the thyroid mass. Although the thyroid absorbed dose for inhalation is substantially (30 to 70%) less than that for injection for all radioiodines and at all ages, it is markedly (25%) less for ingestion only for short-lived 132I.

INVESTIGATION OF THE POSSIBILITY OF EARLY DETERMINATION OF THE FATTENING CAPACITY AND SLAUGHTERING QUALITIES OF PIGS BY APPLYING BIOCHEMICAL TESTS

Drawing upon information from literature and relying on some knowledge we possess, in our work we attempted to establish whether growth intensity in pigs, their fattening capacity and slaughtering qualities could be associated with some biochemical qualities of their blood, such as: alkaline phosphatase activity (AP), levels of protein-bound iodine (PBI) and tyrosine, levels of insulin and glychemic and insulin reaction to oral loading with glucose, as well as histomorphological characteristics of thyroid and endocrine pancreas. The tests were performed on Swedish Landrace pigs (n=21) of both sexes, which were divided into two groups on the basis of insulin level in their morning blood sample: group A (n=9) with lower, and group B (n=12) with higher level of insulin. When fattening was finished the animals were slaughtered and then slaughtering qualities and histomorphological characteristics of thyroid and endocrine pancreas determined. The performed investigation showed that some differences in the studied characteristics exist between the groups of pigs of the same breed. However, the obtained results are not of such weight that would allow drawing conclusions of sound and reliable character pertaining to the possible association of chemical qualities of blood with fattening and slaughtering qualities.