Protein Absorption Research Articles

Endophytic Enterobacter sp. YG-14 mediated arsenic mobilization through siderophore and its role in enhancing phytostabilization

Soil arsenic (As) phytoremediation has long faced the challenge of efficiently absorbing As by plant accumulators while maintaining their health and fast growth. Even at low doses, arsenic is highly toxic to plants. Therefore, plant growth-promoting microorganisms that can mediate As accumulation in plants are of great interest. In this study, the endophyte Enterobacter sp. YG-14 (YG-14) was found to have soil mobilization activity. By constructing a siderophore synthesis gene deletion mutant (ΔentD) of YG-14, the endophyte was confirmed to effectively mobilize Fe-As complexes in mining soil by secreting enterobactin, releasing bioavailable Fe and As to the rhizosphere. YG-14 also enhances As accumulation in host plants via extracellular polymer adsorption and specific phosphatase transfer protein (PitA) absorption. The root accumulation of As was positively correlated with YG-14 root colonization. In addition, YG-14 promoted plant growth and alleviated oxidative damage in R. pseudoacacia L. under arsenic stress. This is the first study, from phenotype, physiology, and molecular perspectives, to determine the role of endophyte in promoting As phytostabilization and maintaining the growth of the host plant. This demonstrated the feasibility of using endophytes with high siderophore production to assist host plants in As phytoremediation.

Anti-tumor target screening of sea cucumber saponin Frondoside A: a bioinformatics and molecular docking analysis.

Cancer remains the leading cause of death worldwide. In spite of significant advances in targeted and immunotherapeutic approaches, clinical outcomes for cancer remain poor. The aim of the present study was to investigate the potential mechanisms and therapeutic targets of Frondoside A for the treatment of liver, pancreatic, and bladder cancers. The data presented in our study demonstrated that Frondoside A reduced the viability and migration of HepG2, Panc02, and UM-UC-3 cancer cell in vitro. Moreover, we utilized the GEO database to screen and identify for differentially expressed genes (DEGs) in liver, pancreatic, and bladder cancers, which resulted in the identification of 714, 357, and 101 DEGs, respectively. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation were performed using the Metascape database for DEGs that were significantly associated with cancer development. The protein-protein interaction (PPI) networks of the identified DEGs in liver, pancreatic, and bladder cancers were analyzed using Cytoscape 3.9.0 software, and subsequently identified potential key genes that were associated with these networks. Subsequently, their prognostic values were assessed by gene expression level analysis and Kaplan-Meier survival analysis (GEPIA). Furthermore, we utilized TIMER 2.0 to investigate the correlation between the expression of the identified key gene and cancer immune infiltration. Finally, molecular docking simulations were performed to assess the affinity of Frondoside A and key genes. Our results showed a significant correlation between these DEGs and cancer progression. Combined, these analyses revealed that Frondoside A involves in the regulation of multiple pathways, such as drug metabolism, cell cycle in liver cancer by inhibiting the expression of CDK1, TOP2A, CDC20, and KIF20A, and regulates protein digestion and absorption, receptor interaction in pancreatic cancer by down-regulation of ASPM, TOP2A, DLGAP5, TPX2, KIF23, MELK, LAMA3, and ANLN. While in bladder cancer, Frondoside A regulates muscle contraction, complement and coagulation cascade by increase FLNC expression. In conclusion, the present study offers valuable insights into the molecular mechanism underlying the anticancer effects of Frondoside A, and suggests that Frondoside A can be used as a functional food supplement or further developed as a natural anti-cancer drug.

Improved emulsifying properties of water-soluble myofibrillar proteins at acidic pH conditions: Emphasizing pH-regulated electrostatic interactions with chitosan

Water-soluble muscle protein with enhanced functionalities has attracted great interest for low-salt food design. Electrostatic interactions of chitosan (CS) with myofibrillar proteins (MP) in water-aqueous solution at acidic pHs (4.0–6.5) were investigated, and how pH regulated complex formation, microstructures, conformation changes, and emulsifying capacity was systematically explored. At pH 4.0–4.5, MP and CS were positively charged and displayed a co-soluble system, exhibiting small particles and high solubility. When the pH increased to near the isoelectric point (pI) of MP (pH 5.0–6.0), electrostatic interactions largely inhibited the aggregation of MP by forming smaller particle complexes. The flexible structures and improved amphiphilic properties promoted protein absorption at the oil-water interface, further improving the emulsion stability. When the pH increased to 6.5, large aggregates were formed causing poor functionalities. This study could provide great insights to further exploit meat-protein-based low-salt functional foods in novel food design.

Genetic and transcriptome analysis of the smb mutant in Bombyx mori

More than 600 mutations have been discovered in the history of silkworm domestication. It is important to study the formation mechanism of these mutations to further understand the life and development process of silkworms and agricultural pest control. The silkworm mutant smb was isolated from silkworm strain NCV, and transcriptome analysis was performed on the silkworm mutant. 796 differentially expressed genes (DEGs) were detected at 48 h of the second instar stage with 669 genes significantly upregulated and 127 genes significantly downregulated. During the GO enrichment analysis, it was found that the enrichment of biological processes was mainly concentrated in proteolysis, carbohydrate metabolism, aminoglycan metabolism, organic substance metabolism, protein metabolism and so on. Based on the analysis of KEGG pathways, it revealed that the pathways enriched in lysosomes, AMPK signaling, fatty acid metabolism, PPAR signaling, galactose metabolism, and protein digestion and absorption were the most significant. Through these most significantly enriched GO terms and KEGG pathways, DEGs consistent with the phenotypic characteristics of the smb mutant were identified, including small body size, slow development, and successive death after the fourth instar. These results provided experimental evidence for the potential formation mechanism of smb mutants.

Identification of differentially expressed genes associated with the pathogenesis of gastric cancer by bioinformatics analysis

AimGastric cancer (GC) is one of the most diagnosed cancers worldwide. GC is a heterogeneous disease whose pathogenesis has not been entirely understood. Besides, the GC prognosis for patients remains poor. Hence, finding reliable biomarkers and therapeutic targets for GC patients is urgently needed.MethodsGSE54129 and GSE26942 datasets were downloaded from Gene Expression Omnibus (GEO) database to detect differentially expressed genes (DEGs). Then, gene set enrichment analyses and protein-protein interactions were investigated. Afterward, ten hub genes were identified from the constructed network of DEGs. Then, the expression of hub genes in GC was validated. Performing survival analysis, the prognostic value of each hub gene in GC samples was investigated. Finally, the databases were used to predict microRNAs that could regulate the hub genes. Eventually, top miRNAs with more interactions with the list of hub genes were introduced.ResultsIn total, 203 overlapping DEGs were identified between both datasets. The main enriched KEGG pathway was “Protein digestion and absorption.” The most significant identified GO terms included “primary alcohol metabolic process,” “basal part of cell,” and “extracellular matrix structural constituent conferring tensile strength.” Identified hub modules were COL1A1, COL1A2, TIMP1, SPP1, COL5A2, THBS2, COL4A1, MUC6, CXCL8, and BGN. The overexpression of seven hub genes was associated with overall survival. Moreover, among the list of selected miRNAs, hsa-miR-27a-3, hsa-miR-941, hsa-miR-129-2-3p, and hsa-miR-1-3p, were introduced as top miRNAs targeting more than five hub genes.ConclusionsThe present study identified ten genes associated with GC, which may help discover novel prognostic and diagnostic biomarkers as well as therapeutic targets for GC. Our results may advance the understanding of GC occurrence and progression.

Influence of Water Stress on Expression of the X95950 Gene and Several Cellular Callus Components in Violet-Treated Sunflower Varieties

The research was conducted between 2017 and 2019 at the College of Agricultural Engineering Sciences and Laboratory of Plant Tissue Culture for Postgraduate Studies at the University of Baghdad. One experiment used a totally random design. The experiment examined the effects of PEG (Polyethylene glycol) at concentrations of 0, 2, 4, 6, and 8% on the development of three sunflower types (Ishaqi-1, Aqmar, and AL-Haja) exposed to UV-C rays for 40 minutes as a result of the growing of the juvenile peduncle outside the live body. The aim of the study was to better comprehend the physiological and biochemical changes caused by water stress on the callus of several sunflower varieties. The X95950 gene was amplified using qPCR technology to investigate drought tolerance gene expression and callus cell content of glutathione, ascorbic acid, and APX enzyme as indicators to determine the effect of PEG on callus tissue cells in the nutrient medium. The expression of the X95950 gene was influenced by water stress. It was particularly expressed in Ishaqi-1 at 4 and 8% PEG concentrations, with values of 1.64 and 1.01, respectively. The glutathione content and activity of the enzyme ascorbate peroxidase, which were 56.49 mol-1 and 0.149 mg-1 protein absorption units, differed significantly among the varieties. PEG concentrations significantly influenced glutathione content, with 8% achieving the highest average of 50.07 mol g-1 and 4% achieving the highest averages of ascorbic acid and ascorbate peroxidase enzyme activity of 2.462 mg 100 g-1 and 0.138 mg-1 protein absorption units, respectively. The interaction was considerable, with the maximum average glutathione callus content in the Aqmar variety reaching 69.60 mol g-1 at 6% PEG concentration. At 4% PEG concentration, the Aqmar variety outperformed in terms of APX enzyme efficacy, reaching 0.238 mg-1 absorption units of protein.

Blood Transcriptome Analysis Provides Responsive Changes in Gene Expression between Ex Situ and Captive Yangtze Finless Porpoises (Neophocaena asiaeorientalis asiaeorientalis)

The Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis, YFP) is an endangered species endemic to the Yangtze River in China, and it is the only freshwater whale in the genus Neophocaena. In terms of protection, three effective conservation strategies exist: in situ conservation, ex situ conservation, and artificial breeding, all of which have been implemented by the Chinese government. Of these, ex situ conservation involves the relocation of Yangtze finless porpoises to semi-natural waters with less human interference, and artificial breeding involves the relocation of Yangtze finless porpoises to a controlled environment that is more strictly managed. To compare and analyze the responsive changes in gene expression of the YFPs between the ex situ and controlled environments, we performed the RNA sequencing of blood tissues from these YFPs. A total of 1201 differentially expressed genes (DEGs) were identified, of which 423 were up-regulated in the ex situ population and 778 were up-regulated in the controlled-environment population. Gene enrichment analysis showed that 1201 DEGs between the ex situ and controlled-environment populations were generally enriched for vision-, digestion- and immune-system-related pathways. Further analysis revealed that several key immune system pathways, such as the chemokine signaling pathway and B cell receptor signal pathway, were activated in the ex situ population. In addition, the key pathways related to vision, including phototransduction and the inflammatory mediator regulation of TRP channels, as well as the pathways related to the digestive system, such as protein digestion and absorption and salivary secretion, were activated in the controlled-environment population. These results suggest that the ex situ populations may respond to complex environmental conditions in semi-natural waters by enhancing their immune function through the increased expression of immune-related genes and that the visual function and protein digestion of the YFPs were improved compared to those of the ex situ population based on the conditions of artificial feeding, such as the higher transparency of the water and regular feeding. This study provides clues for evaluating the adaptability of YFPs to different environments and is a useful reference for future ex situ conservation and artificial breeding.

Bacterial cellulose nanofibrous aerogels grafted with citric acid for absorption and separation of protein

Bacterial cellulose nanofibrous aerogels grafted with citric acid for absorption and separation of protein

Responses of the intestinal microbiota to exposure of okadaic acid in marine medaka Oryzias melastigma

It is still limited that how the microalgal toxin okadaic acid (OA) affects the intestinal microbiota in marine fishes. In the present study, adult marine medaka Oryzias melastigma was exposed to the environmentally relevant concentration of OA (5 μg/L) for 10 days, and then recovered in fresh seawater for 10-days depuration. Analysis of taxonomic composition and diversity of the intestinal microbiota, as well as function prediction analysis and histology observation were carried out in this study. Functional prediction analysis indicated that OA potentially affected the development of colorectal cancer, protein and carbohydrate digestion and absorption functions, and development of neurodegenerative diseases like Parkinson’s disease, which may be associated with changes in Proteobacteria and Firmicutes in marine medaka. Significant increases of C-reactive protein (CRP) and inducible nitric oxide synthase (iNOS) levels, as well as the changes of histology of intestinal tissue demonstrated that an intestinal inflammation was induced by OA exposure in marine medaka. This study showed that the environmental concentrations of OA could harm to the intestinal microbiota thus threatening the health of marine medaka, which hints that the chemical ecology of microalgal toxins should be paid attention to in future studies.

Effects of endogenous lipid on the functional qualities of tuna protein during cold storage

The effects of endogenous lipid on the quality of tuna protein during storage was evaluated. Defatted tuna protein was prepared and co-stored with different contents of tuna oil or soybean oil, and the functional qualities, oxidation, protein structure and lipid oxidation were compared. During storage, a higher tuna oil content caused a greater attenuation in the functional qualities of protein, including emulsifying, foaming and water absorption abilities, and tuna oil could lead to more severe attenuation than soybean oil. Besides, the higher the oxidation degree of oil, the higher the oxidation degree and the more disordered of sarcoplasmic and myofibrillar proteins, which indicated that protein oxidation could be induced by lipid oxidation and cause greater structural damage. Finally, Pearson correlation revealed that variation in myofibrillar protein more strongly affects the functional qualities than sarcoplasmic protein. Therefore, the removal of endogenous lipid had a significant positive impact on the quality stability of protein products during storage, especially for marine protein products.

Protein turnover in pigs: A review of interacting factors.

Protein turnover defines the balance between two continuous and complex processes of protein metabolism, synthesis and degradation, which determine their deposition in tissues. Although the liver and intestine have been studied extensively for their important roles in protein digestion, absorption and metabolism, the study of protein metabolism has focused mainly on skeletal muscle tissue to understand the basis for its growth. Due to the high adaptability of skeletal muscle, its protein turnover is greatly affected by different internal and external factors, contributing to carcass lean-yield and animal growth. Amino acid (AA) labelling and tracking using isotope tracer methodology, together with the study of myofiber type profiling, signal transduction pathways and gene expression, has allowed the analysis of these mechanisms from different perspectives. Positive stimuli such as increased nutrient availability in the diet (e.g., AA), physical activity, the presence of certain hormones (e.g., testosterone) or a more oxidative myofiber profile in certain muscles or pig genotypes promote increased upregulation of translation and transcription-related genes, activation of mTORC1 signalling mechanisms and increased abundance of satellite cells, allowing for more efficient protein synthesis. However, fasting, animal aging, inactivity and stress, inflammation or sepsis produce the opposite effect. Deepening the understanding of modifying factors and their possible interaction may contribute to the design of optimal strategies to better control tissue growth and nutrient use (i.e., protein and AA), and thus advance the precision feeding strategy.

Zwitterionic Polymer-Decorated Lipid Nanoparticles for mRNA Delivery in Mammalian Cells.

Lipid nanoparticles (LNPs) play a key role in the effective transport of mRNA into cells for protein translation. Despite the stealthiness of poly(ethylene glycol) (PEG) that helps protect LNPs from protein absorption and blood clearance, the generation of anti-PEG antibodies resulting in PEG allergies remains a challenge for the development of an mRNA vaccine. Herein, a non-PEG lipid was developed by conjugating 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) with an antifouling zwitterionic polymer, poly(2-methyacryloyloxyethyl phosphorylcholine) (PMPC), of different chain lengths. The PMPC-LNPs formulated from DPPE-PMPC were spherical (diameter ≈ 144-255 nm), neutral in charge, and stable at 4 °C for up to 28 days. Their fraction of stealthiness being close to 1 emphasized the antifouling characteristics of PMPC decorated on LNPs. The PMPC-LNPs were nontoxic to HEK293T cells, did not induce inflammatory responses in THP-1 cells, and exhibited an mRNA transfection efficiency superior to that of PEG-LNPs. This work demonstrated the potential of the developed zwitterionic polymer-conjugated LNPs as promising mRNA carriers.

A novel strategy for key gene identification in hypertrophic cardiomyopathy based on cuproptosis and multiple WGCNA analyses

Abstract Background Hypertrophic cardiomyopathy (HCM) is a potentially fatal disease, and early diagnosis is crucial for effective treatment. Microarray analysis and the discovery of cuproptosis, a new form of cell death, offer new possibilities for identifying potential biomarkers for early diagnosis. Purpose This study aimed to identify cuproptosis-related genes associated with HCM and potential biomarkers for early diagnosis using machine learning. Methods Expression data of cuproptosis-related genes were extracted from the GSE36961 dataset, and differential expression analysis was conducted. Immune infiltration analyses were also performed. Samples were categorized into two clusters, and cluster weighted gene coexpression network analysis (WGCNA) was performed based on HCM and cluster clinical data. The results were combined to develop the best machine learning model, which was verified through calibration and external validation (GSE1145 and GSE32453). Results Cuproptosis-related genes (NLRP3, ATP7B, ATP7A, SLC31A1, LIAS, LIPT1, DLD, DLAT, PDHB, and DBT) were differentially expressed between HCM and control samples. Five key genes (TSPAN12, ANP32C, C4ORF18, COL21A1, and HMGB2) were identified, which showed great efficiency in the diagnosis of HCM in external validation, with an AUC of 0.948. These genes may play critical roles in megakaryocyte differentiation, RAGE receptor binding, and protein digestion and absorption. Conclusions Cuproptosis-related genes may play significant roles in HCM, and TSPAN12, ANP32C, C4ORF18, COL21A1, and HMGB2 may be potential biomarkers for the early diagnosis of HCM. This study provides new insights for further research on HCM diagnosis and treatment.Flow chart of research designEvaluation and verification results

201 Effect of Spray Dried Plasma Compared with Egg White Or Whole Egg as Functional Binders in Canned Pet Food

Abstract Many different binders are used in canned pet food providing functionality such as gelling, texture, and protein content. Spray dried plasma (SDP) is a consistent high protein ingredient commonly utilized in wet pet food to provide texture and for water binding and fat emulsification characteristics. The objective of the study was to evaluate functionality of three different binders: egg white, whole egg, and SDP in chunks recipes at four inclusions levels (0, 2, 4, and 6%.) Canned petfood batches were replicated 3 times with each condition using the same recipe containing different raw materials, mainly derived from chicken and pigs, with control (no binder) and level of all binders in each replication. Thus, 3 batches per binder and level were produced for a total of 9 batches. The binder was included to partially replace poultry carcass in the chunks. Cans of chunks and gravy were produced with the ratio of 50:50 chunks to gravy and cooked at 121°C for 1 hour. Cans were stored at room temperature for 14 days before product measurements. Within each batch, 6 to 9 cans were evaluated for protein, texture, hardness, springiness, cohesiveness, chewiness, and gravy absorption. Protein was similar at 2% inclusion between binders. At 4% binder inclusion, protein was greatest (P < 0.05) with SDP and egg white compared with whole egg and control; while 6% inclusion resulted in greatest (P < 0.05) protein with egg white followed by SDP, whole egg, and control. Texture was increased (P < 0.05) with SDP and egg white compared with whole egg and control at 2% and 6%, while at 4% inclusion SDP was the highest (P < 0.05) compared with all other binders. Hardness was greatest (P < 0.05) with SDP at 2 and 4% compared with all binders; while at 6% inclusion egg white was greatest followed by SDP, whole egg, and control. Cohesiveness, springiness, and chewiness were greatest with SDP at all levels compared with other binders. Gravy absorption into the chunks with 2% binder inclusion was increased (P < 0.05) with SDP and egg white, similar between all binders at 4% but greater than control, and greatest with egg white at 6% followed by SDP, whole egg, and control. Overall, greater amounts of binder increased protein, hardness, texture, and gravy absorption. Spray dried plasma may be used as an alternative for egg white and whole egg in canned pet food.

Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain

Proinsulin Like Growth Factor I (prolGF-I) and myostatin (Mstn) regulate muscle regeneration and mass when intravenously delivered. We tested if chloroplast bioencapsulated forms of these proteins may serve as a non-invasive means of drug delivery through the digestive system. We created tobacco (Nicotiana tabacum) plants carrying GFP-Fc1, proIGF-I-Fc1, and Mstn-Fc1 fusion genes, in which fusion with the immunoglobulin G Fc domain improved both protein stability and absorption in the small intestine. No transplastomic plants were obtained with the Mstn-Fc1 gene, suggesting that the protein is toxic to plant cells. proIGF-I-Fc1 protein levels were too low to enable in vivo testing. However, GFP-Fc1 accumulated at a high level, enabling evaluation of chloroplast-made Fc fusion proteins for oral delivery. Tobacco leaves were lyophilized for testing in a mouse system. We report that the orally administered GFP-Fc1 fusion protein (5.45 µg/g GFP-Fc1) has been taken up by the intestinal epithelium cells, evidenced by confocal microscopy. GFP-Fc1 subsequently entered the circulation where it was detected by ELISA. Data reported here confirm that chloroplast expression and oral administration of lyophilized leaves is a potential delivery system of therapeutic proteins fused with Fc1, with the advantage that the proteins may be stored at room temperature.

Comparative analysis in the prediction of early-stage diabetes using multiple machine learning techniques

<span>Diabetes is caused by high levels from blood glucose and it is characterized as a chronic disease, and also will disrupt fat and protein absorption. <br /> The levels rise from blood glucose because it cannot be burned in the cells from the pancreas because of the deficiency of insulin secretion or the insulin produce by the cell are insufficient. By means of early detection, it may decrease the hazards and frequency of diabetes. The application of technology has been an essential part of providing accurate and acceptable results in the prevention and early detection of the illness. This research provided the best machine learning used for predicting the early stage of diabetes. The methods involve the feature selection or dimension reduction using relief-based filter (reliefF), tenfold cross-validation for testing and training data, and different machine learning classifiers such as the random forest (RF), support vector machines (SVM), and neural network (NN) is used. in this research, <br /> RF recorded the highest precision point at 98.5%, which was able to provide a higher evaluation in terms of accuracy followed by SVM at 96.6% and NN at 96.2%. The results generated from this experiment are essential in contributing a new way that is highly accurate in determining diabetes <br /> among patients.</span>

4D DIA-PRM proteomic study identifying modulated pathways and biomarkers associated with pelvic organ prolapse

Pelvic organ prolapse (POP) is a highly disabling condition that negatively affects the quality of life of millions of women worldwide. However, the underlying mechanisms associated with the development and progression of the disease remain poorly understood. Here, an untargeted four-dimensional data-independent acquisition (4D DIA)-based proteomics approach was applied to vaginal wall tissue samples from POP (n = 19) and control (n = 8) patients to identify potential diagnostic biomarker(s) for POP and examine the molecular mechanisms underlying the disease. Of the 5713 tissue proteins that were detected, 1957 proteins were significantly changed in POP patients. Further bioinformatics analysis revealed that multiple biological processes including protein digestion & absorption, retrograde endocannabinoid signaling, tyrosine metabolism, and nucleotide metabolism were significantly enriched and associated with the pathogenesis of POP. Interestingly, 16 of these differentially expressed proteins associated with four pathways were also identified by targeted parallel reaction monitoring (PRM) proteomics analysis on the same 27 tissue samples. Changes in 94 % (15/16) of these proteins were consistent with the 4D DIA data. Furthermore, most proteins displayed good diagnostic accuracy with high area under the curve (AUC) values (AUC＞0.8). Specifically, five proteins including ELN, COL6A2, ENTPD1, AOC3, and COX7A2 distinguished between POP and control patients with very high accuracy (AUC ≥ 0.95) in both 4D DIA and PRM analyses, and may therefore be potential diagnostic biomarkers for POP. In summary, the present study not only provided several potential biomarker(s) for effective POP diagnosis, but extended our knowledge of the key regulatory pathways associated with the disease.

Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

The digestive process and intestinal protein absorption are influenced by a variety of factors. Due to their numerous health advantages, including potential favorable effects on protein digestion and absorption, probiotics have gained increased attention in recent years. Probiotics can control the intestinal microflora, which in turn affects the intestinal bacteria responsible for proteolysis. Additionally, certain probiotics can release exoenzymes that aid in the digestion of proteins and others can stimulate the host’s digestive protease and peptidase activity. By boosting transport and enhancing the epithelium’s capacity for absorption, probiotics can also improve the absorption of tiny peptides and amino acids as well as lessen detrimental protein fermentation, which lowers the toxicity of metabolites. The present study explored the production of enzymes by Bacillus paranthracis strain MHDS3, a probiotic candidate isolated from Pellaea calomelanos. Bacillus paranthracis displayed enzyme activities of amylase (31,788.59 IU), cellulase (4487.486 IU), and pectinase (13.98986 IU) through submerged fermentation. The CAZyme analysis of B. paranthracis revealed 16 CAZyme gene clusters associated with cellulose, amylase, and pectinase activity. Thus, B. paranthracis is a promising probiotic strain that can produce enzymes with biotechnological applications.

Roles of DSCC1 and GINS1 in gastric cancer.

Gastric carcinoma is a common malignant tumor originating from gastric mucosal epithelium. However, role of DS-cell cycle-dependent protein 1 (DSCC1) and GINS1 in gastric carcinoma remains unclear. The gastric carcinoma datasets GSE79973 and GSE118916 were downloaded from gene expression omnibus. Multiple datasets were merged and batched. Differentially expressed genes (DEGs) were screened and weighted gene co-expression network analysis was performed. Functional enrichment analysis, gene set enrichment analysis and immune infiltration analysis were performed. Construction and analysis of protein-protein interaction Network. Survival analysis and comparative toxicogenomics database were performed. A heat map of gene expression was drawn. Target Scan screen miRNAs regulating DEGs. Two thousand forty-four DEGs were identified. According to gene ontology analysis, in biological process, they were mainly enriched in cell migration, transforming growth factor β receptor signaling pathway, angiogenesis, and steroid metabolism process. In cellular component, they were mainly enriched in extracellular vesicles, basement membrane, endoplasmic reticulum lumen, and extracellular space. In molecular function, they focused on extracellular matrix structural components, protein binding, platelet-derived growth factor binding, and catalytic activity. In Kyoto encyclopedia of genes and genomes, they were mainly enriched in protein digestion and absorption, metabolic pathways, fatty acid degradation, Glycerophospholipid metabolism, ether lipid metabolism. Gene set enrichment analysis showed that DEGs were mainly enriched in transforming growth factor β receptor signaling pathway, steroid metabolism process, basement membrane, endoplasmic reticulum lumen, structural components of extracellular matrix, platelet-derived growth factor binding, Glycerophospholipid metabolism, ether lipid metabolism. The results of immune infiltration analysis showed that expression of T cell CD4 memory resting was lower in the samples of gastric cancer. The core genes (TRIP13, CHEK1, DSCC1, GINS1) are protective factors, their expression shows a downward trend with increase of risk score. Comparative toxicogenomics database analysis showed that TRIP13, CHEK1, DSCC1, GINS1 were related to gastric tumors, gastric diseases, tumors, inflammation, and necrosis. DSCC1 and GINS1 are highly expressed in gastric cancer. Higher expression levels of DSCC1 and GINS1, worse the prognosis.

Papaya Fruit Processing as Meat Tenderizer in Ponggok Village, Blitar

Papaya is one of the largest commodities in Ponggok Village, Blitar Regency. However, the papaya is only sold and consumed as a fresh fruit. Papaya sap contains protease enzymes (protein decomposers), papain and chymopapain, that can hydrolyze proteins. This protease enzyme is a key factor in increasing protein digestibility and absorption, which in turn affects growth of tissue fibers in meat. These two enzymes can break down the bonds in protein molecules into polypeptides and dipeptides. The purpose of this activity is to provide training on making papain enzymes from papaya fruit. The papain enzyme is used as a meat tenderizer. The target of the training participants was thirty people with the target being PKK women who were members of the Ponggok village farmer group. The method in this training uses the stages of preparation, implementation and evaluation. The survey results show that papaya fruit has not been utilized optimally in Ponggok Village, so it is important to hold training on making papain enzyme from papaya fruit.