Prostrate Spurge Research Articles

Evaluation of segmentation and semi‐supervised learning for spotted spurge recognition in bermudagrass turf

AbstractDiverse datasets are crucial for training machine learning‐based weed recognition models. However, annotating (i.e., labeling) images can be laborious and time‐consuming. Choice of annotation method and training approach not only affects the overall model effectiveness but also its minimum training data requirements and development pace. Segmentation and semi‐supervised learning (SSL) may offer performance or training enhancements. This study evaluated (1) segmentation against object detection in spotted spurge [Chamaesyce maculata (L.) Small] recognition in Latitude 36 hybrid bermudagrass [Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt‐Davy] maintained as a golf course fairway and (2) the potential of two‐step SSL‐based training procedure using data labeled both manually and automatically with a pre‐trained model to expedite the model development. The architecture used across this research was You Only Look Once version 8 (YOLOv8) employing nano, small, and medium variants. All models were trained with a dataset restricted to 1200 training and 300 validation images. Both annotation methods resulted in adequate spotted spurge identification, as evidenced with >0.60 (>0.50 acceptability threshold) medium average precision at intersection over union threshold of 0.50 (mAP@50). Although the difference was minimal, object detection performed superior to segmentation. The two‐step training effectively accelerated image annotation while preserving or improving (with 8:4 and 6:6 splits between manually and auto‐labeled data) object detection performance. Segmentation tolerated only 10:2 split and exhibited increased sensitivity to declining dataset quality proportionally to the increase in auto‐labeled images in the final training dataset. Findings show a two‐step SSL‐based training procedure expedites annotation, enhancing model development efficiency.

Euphorbia supina Extracts Block NADPH Oxidase-Mediated, Ceramide-Induced Apoptosis Initiated by Diesel Particulate Matter.

Air pollutants contribute to the development of diseases such as asthma, chronic obstructive pulmonary disease (COPD), pulmonary cancer, cardiovascular problems, and some skin diseases. We recently found that a major air pollutant, diesel particulate matter (DPM), induces apoptosis in human keratinocytes by increasing a proapoptotic lipid mediator, ceramide. DPM activates nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX), which stimulates sphingomyelinase, leading to an increased conversion of sphingomyelin to ceramide. Interestingly, we characterized that although NOX is a reactive oxygen species (ROS) generator, the activation of sphingomyelinases by NOX is an ROS-independent mechanism. A Korean weed, prostrate spurge Euphorbia supina Rafin (ESR), has been used for centuries as a folk medicine to treat bronchitis, hepatitis, hemorrhage, and skin inflammation. Flavonoids, terpenes and tannins are enriched in ESR, and although ESR has proven antioxidative activity, its biological activities are largely unknown. Here, we investigate whether and how ESR protects keratinocytes against DPM-mediated apoptosis. We found that ESR-extracts (ESR-Ex) protect keratinocytes from DPM-induced apoptosis by inhibiting NOX activation in keratinocytes in response to DPM. We also demonstrated that ESR-Ex suppresses NOX activation via a blockage of the aryl hydrocarbon receptor (AhR) activation-mediated transcription of neutrophil cytosolic factor 1 (NCF1)/p47phox, a subunit of NOX. Our study reveals previously uncharacterized biological activity of ESR-Ex; i.e., its inhibition of Ahr and NOX activation. Thus, because the inhibition of NOX has already been developed to treat NOX-mediated diseases, including various types of cardiovascular diseases and cancers, initiated by air pollutants and because AhR activation contributes to the development of chronic inflammatory diseases, our study provides further advantages for the medical use of ESR.

Thirty‐six years of no‐tillage regime altered weed population dynamics in soybean

AbstractChanges to tillage practices can impact weed species composition and population dynamics in arable fields. The objective of this study was to evaluate and compare the impact of long‐term (36 yr) no‐tillage (NT) and conventional‐tillage (CT) systems on weed species composition, density, seedling emergence, and diversity, in a continuous soybean [Glycine max (L.) Merr.] system in Southeast Texas. Results from 2016 and 2017 observations showed that weed species composition varied between CT and NT, and the total density was greater in NT (14 and 86 plants m–2 for summer and winter annuals, respectively) compared to CT (3 and 45 plants m–2, respectively). Moreover, tall waterhemp [Amaranthus tuberculatus (Moq.) Sauer], prostrate spurge [Chamaesyce humistrata (Engelm. ex Gray) Small], and red sprangletop [Dinebra panicea (Retz.) P.M. Peterson & N. Snow] emergence was delayed in NT compared to CT. Vertical distribution (70‐cm depth) of viable weed seeds in the soil profile was also influenced by tillage regime; greater proportion of weed seeds were present on the soil surface (0–5 cm) in NT (57–80% among different species) compared to CT (38–56%). However, weed diversity indices did not differ between CT and NT. Results indicate that long‐term NT, even with herbicide management, can lead to greater weed densities with a shift towards small‐seeded annual species (common purslane [Portulaca oleraceae L.], parsley‐piert [Aphanes arvensis L.], cutleaf groundcherry [Physalis angulate L.]). Growers transitioning to NT should be cognizant of potential changes to weed population dynamics as a result of altered tillage regime and devise strategies for effective management.

No-tillage altered weed species dynamics in a long-term (36-year) grain sorghum experiment in southeast Texas

AbstractTillage regimes can influence weed population dynamics and, consequently, the choice of appropriate weed management practices. Studies were conducted in 2016 and 2017 in a long-term (36-yr) grain sorghum [Sorghum bicolor (L.) Moench ssp. bicolor] experiment at Texas A&M University, College Station, to determine the impact of long-term no-till (NT) and conventional till (CT) systems on weed species dynamics. Higher densities of johnsongrass [Sorghum halepense (L.) Pers.], prostrate spurge [Chamaesyce humistrata (Engelm. ex A. Gray) Small], waterhemp [Amaranthus tuberculatus (Moq.) Sauer], and henbit (Lamium amplexicaule L.) were recorded in the NT system compared with the CT system. Further, the NT system showed greater weed diversity (Shannon-Wiener index, H = 0.8) and species richness (S = 6.2), compared with the CT system (H = 0.6, S = 4.2). Seedling emergence of some dominant weed species was also delayed in the NT system. In the CT system, 50% emergence of S. halepense (8.5 C base temperature) and waterhemp (10 C base temperature) occurred at 59 and 63 growing degree days (GDD), respectively, whereas 68 and 75 GDD, respectively, were required in the NT system. Further, a greater proportion (61%) of the viable seedbank was present at the top 5 cm of the soil in the NT system compared with the CT system (46%). Overall, findings from this 36-yr-long tillage experiment have revealed that the NT system had greater weed densities (especially of the perennial weed S. halepense) and a high proportion of weed seeds (particularly small-seeded annuals) on the topsoil layer, corroborating some earlier reports that were based on short-term investigations. Findings indicate that growers transitioning to NT systems should be mindful of potential shifts in weed species dominance and develop appropriate management tactics.

Seed Longevity of Spotted Spurge and Wild Poinsettia Under Diverse Environmental Conditions

ABSTRACT: Seed longevity under different environmental conditions is considered as one of the most important factors in the spread and persistence of an exotic species. The Experiments were conducted to determine seed persistence in soil, effects of submergence in water, flooding of the soil, and high temperatures on germination and viability of spotted spurge (Chamaesyce maculata) and wild poinsettia (Euphorbia heterophylla) as two exotic species in different regions of Golestan province. Spotted spurge seeds buried at depth of 10 cm maintained their viability above 95% after a year, while wild poinsettia seeds were destroyed completely after exhuming the soil. Seeds of both species were unable to germinate under submergence, but 92% of the spotted spurge seeds remained viable under this condition for 14 d. No germination was observed after 9 weeks submersion. Submersion duration drastically affected seed germination of wild poinsettia, so that no germination occurred after 6 d submersion. Twelve days after flooding, spotted spurge emergence decreased by 57% compared to the control. Ten percentage of wild poinsettia seedlings emerged when flooding was kept up to 12 d after sowing, while control had 96% emergence. Germination of spotted spurge seeds subjected to 140 oC for 5 min was 5%. Viability of wild poinsettia seed was completely lost at 120 and 140 C for 5 min. These results suggest that spotted spurge is capable of forming persistent seedbank. Seeds of spotted spurge were partially tolerant to submersion in water, but wild poinsettia seed are susceptible to submergence. The burning of crop residue could also prevent augmenting the soil seed bank of both species.

Dimethenamid Persistence and Leaching Potential in a Soilless Mix1

Abstract Dimethenamid and the granular combination product pendimethalin plus dimethenamid are herbicides registered for use in nursery production and landscape maintenance. The objectives of this study were: (1) to compare the effectiveness of sprayed dimethenamid and the granular combination of pendimethalin plus dimethenamid in container nursery production, (2) to determine the impact of formulation on leaching of dimethenamid in soilless media versus field soil and (3) to determine the influence of irrigation volume on herbicide leaching. Dimethenamid is less mobile in pine bark than field soil, while pendimethalin is more mobile in pine bark. The leaching profile for the granular pendimethalin plus dimethenamid combination product was similar to dimethenamid in field soil and similar to pendimethalin in pine bark. There was no significant difference in herbicide movement in pine bark or field soil after doubling the irrigation volume from 17.8 cm (7 in) to 35.6 cm (14 in). Compared to pendimethalin, dimethenamid leaches less in pine bark, explaining its greater effectiveness for weed control in container production. Index words: Herbicide leaching, soilless mix, pine bark, ornamentals, adsorption. Species used in this study: southern crabgrass [Digitaria ciliaris (Retz.) Koel.]; spotted spurge [Chamaesyce maculata (L.) Small]. Chemicals used in this study: dimethenamid (Tower); dimethenamid plus pendimethalin (FreeHand); pendimethalin (Pendulum 2G).

Mulch Type and Depth Influences Control of Three Major Weed Species in Nursery Container Production

Two commonly used management practices for weed control in container plant production are hand pulling and herbicide applications. There are problems associated with these methods including crop phytotoxicity and environmental concerns associated with off-target movement of herbicides. Other nonchemical weed control methods could reduce herbicide-based environmental concerns, mitigate herbicide-resistance development, and improve the overall level of weed control in container nursery production. Readily available tree-mulch species, eastern red cedar (Juniperus virginiana), ground whole loblolly pine (Pinus taeda), chinese privet (Ligustrum sinense), and sweetgum (Liquidambar styraciflua) were harvested, chipped, and evaluated at multiple depths with and without the herbicide dimethenamid-p. Pine bark mini-nuggets were also evaluated. Mulches were applied at depths of 1, 2, and 4 inches and evaluated over three 30-day periods for their effectiveness in suppressing spotted spurge (Chamaesyce maculata), long-stalked phyllanthus (Phyllanthus tenellus), and eclipta (Eclipta prostrata). After 30 days, herbicide/mulch combinations, as well as mulch treatments alone, had reduced weed fresh weight 82% to 100% with 1 inch of mulch. By 168 days after treatment, dimethenamid-p had lost all efficacy, and mulch depth was the only factor that still had significant effects, reducing spotted spurge fresh weight by 90%, 99.5%, and 100% with depths of 1, 2, and 4 inches, respectively. The economics of mulch weed control will depend on variables such as available time, nursery layout, location, and availability of resources, equipment, among others. Regardless of variable economic parameters, data from this study reveals that any of these potential mulch species applied at a depth of at least 2 inches will provide long-term weed control in nursery container production.

Aportaciones a la flora alóctona de Córdoba y Andalucía Occidental (España)

Se citan por primera vez para la provincia de Córdoba cuatro taxones alóctonos: Amaranthus blitum subsp. emarginatus, Chamaesyce maculata, Onobrychis viciifolia y Proboscidea louisianica, todos ellos poco frecuentes en Andalucía occidental (y en Andalucía en general). Para cada uno de ellos se ha identificado el origen: predominan los procedentes de América del Norte; estatus de invasión (para A. occidental): naturalizado, invasor, casual y casual, respectivamente; estatus de residencia: los cuatro son neófitos; hábitat: predominan los de zonas construidas y tierras cultivadas; vía de introducción: involuntaria excepto en el caso de O. viciifolia, que se ha considerado intencionada; y primera cita conocida (para A. occidental), entre 1886 (O. viciifolia) y 2001 (P. louisianica). Se ha analizado además la dificultad de identificar el estatus de invasión.

Polyphenols from Korean prostrate spurge Euphorbia supina induce apoptosis through the Fas-associated extrinsic pathway and activation of ERK in human leukemic U937 cells.

The Korean prostrate spurge Euphorbia supina (Euphorbiaceae family) has been used as a folk medicine in Korea against a variety of ailments such as bronchitis, hemorrhage, jaundice and multiple gastrointestinal diseases. Polyphenols from Korean E. supina (PES) which include quercetin and kaempferol derivatives have anticancer properties. Hence, we investigated the anticancer effects of PES on U937 human leukemic cells. Firstly, PES significantly inhibited the proliferation of U937 cells in a dose-dependent manner. PES induced accumulation of the sub-G1 DNA content (apoptotic cell population), apoptotic bodies and chromatin condensation and DNA fragmentation in the U937 cells. PES also induced activation of caspase-3, -8 and -9, subsequent cleavage of PARP, and significantly suppressed XIAP, cIAP-1 and cIAP-2 in dose-dependent manner. Furthermore, PES activated Bid, and induced the loss of mitochondrial membrane potential (MMP, ΔΨm) along with upregulation of pro-apoptotic proteins (Bax and Bad), and downregulation of anti-apoptotic proteins (Bcl-2 and Bcl-xL) and cytochrome c release. The Fas receptor was upregulated by PES in a dose-dependent manner, suggesting that the extrinsic pathway was also involved in the PES-induced apoptosis. Moreover, the PES-induced apoptosis was at least in part associated with extracellular signal-regulated kinase (ERK) activation in the U937 human leukemic cells. This study provides evidence that PES may be useful in the treatment of leukemia.

Polyphenol mixtures of Euphorbia supina the inhibit invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells.

The Korean prostrate spurge Euphorbia supina is abundant in polyphenols and has been used as a folk medicine in Korea against a variety of diseases. Thus, we aimed to investigate the effect of polyphenol mixtures of Korean Euphorbia supina (PES) on the invasion and metastasis of highly metastatic breast cancer MDA-MB-231 cells. Firstly, PES showed no cytotoxicity on cancer cells and endothelial cells (ECs) at the doses of 0.1-10 µg/ml, but showed significant cytotoxicity from 50 µg/ml. Thus, we performed subsequent experiments with PES at doses up to 5 µg/ml. PES dose‑dependently suppressed epithelial-mesenchymal transition by downregulating the mesenchymal markers, Snail1 and N-cadherin, showing significant inhibition from 1 and 5 µg/ml, respectively. In addition, PES significantly inhibited MMP-9 activity and LOX release induced by TNF-α at 5 µg/ml. Then, we determined the effect of PES on the expression of adhesion molecules and VE-cadherin phosphorylation. The results showed that PES effectively reduced TNF-α-mediated VCAM-1 expression but not ICAM expression both in the MDA-MB-231 cells and ECs, resulting in the reduced adhesion of MDA-MB-231 to ECs. Finally, PES effectively inhibited MDA-MB-231 cell invasion through ECs, suggesting that PES may serve as a therapeutic agent against cancer metastasis with minimal cytotoxicity to normal cells.

Evaluation of Mulch and Preemergence Herbicide Combinations for Weed Control in High-density Olive (Olea europaea L.) Production

Field experiments were conducted at the Central Texas Olive Ranch in Walburg, TX, in 2011 and 2012 to evaluate the efficacy of mulch and/or preemergence herbicides for weed control in high-density olive (Olea europaea L.) production during orchard establishment. Treatments were initiated on 1 Apr. 2011 and 28 Mar. 2012 and consisted of a nontreated control, isoxaben (2.2 kg a.i./ha), oryzalin (4.5 kg a.i./ha), oxadiazon (3.36 kg a.i./ha), and mesotrione (0.14 kg a.i./ha). Hardwood mulch was applied to half of each plot following herbicide application. Weed counts, combined across species (camphorweed, texas croton, lanceleaf sage, pinnate tansymustard, tumble pigweed, common purslane, and prostrate spurge), were conducted to assess % weed cover at 4 and 12 weeks after treatment (WAT). In 2011, compared with the nonmulched no herbicide treatment, adding mulch reduced weed counts by 23 and increased weed control by 70% 4 WAT. All preemergence herbicide treatments, regardless of mulching regime, resulted in ≥97% weed control 4 WAT with the exception of oryzalin without mulch (91% weed control, 3 weeds/plot). In 2012, compared with the nonmulched no herbicide treatment, adding mulch reduced weed counts by 35 and increased weed control by 64% 4 WAT. Mulching in combination with mesotrione resulted in 100% weed control, significantly greater than mesotrione applied without mulch (98%, 2 weeds/plot) 4 WAT. Oryzalin without mulch resulted in greater weed control (94%, 4 weeds/plot) in 2012 4 WAT; however, this treatment provided the least amount of weed control of all preemergence herbicides tested. By 12 WAT, weed counts were reduced by 21 and 22 in 2011 and 2012, respectively, in response to mulching in the nontreated plots resulting in a 52% and 42% increase in weed control in 2011 and 2012, respectively. Mesotrione was the only treatment affected by mulching regime 12 WAT in 2011 and 2012. Mesotrione in combination with mulch resulted in 100% weed control in 2011 and 2012, while mesotrione without mulch resulted in 93% weed control (3 and 4 weeds/plot) 12 WAT in 2011 and 2012, respectively. Although not statistically significant, isoxaben applied alone in 2011 resulted in 97% weed control (1 weed/plot), while isoxaben in combination with mulch resulted in 94% weed control (3 weeds/plot) 12 WAT. In 2011, oryzalin and oxadiazon resulted in 87% to 92% control, regardless of mulching regime 12 WAT. Weed control in response to isoxaben in 2012 was 95% 12 WAT, regardless of mulching regime. The combination of oxadiazon + mulch resulted in similar weed control (95%, 3 weeds/plot) 12 WAT; however, oxadiazon alone and oryzalin with and without mulch resulted in 87% to 89% weed control. All preemergence herbicides evaluated provided good to excellent weed control. Isoxaben and oryzalin are labeled for use on nonbearing fruit trees or during orchard establishment, while oxadiazon is only labeled for woody ornamentals. Although not labeled for use in orchards, mesotrione may be an alternative for use in olive production. The addition of mulching did not increase weed control except when used in conjunction with mesotrione. Mulch alone provided moderate weed control when preemergence herbicides were not applied. Furthermore, the utilization of mulch in combination with preemergence herbicides may help reduce photodegradation and/or volatilization when irrigation/rainfall is limited.

Preemergence Control of Spotted Spurge (Chamaesyce maculata) with Flumioxazin as Influenced by Formulation and Activation Moisture

Flumioxazin is commonly used in nursery production for PRE weed control. Container nursery producers are of the opinion that the granular formulation is less effective than the sprayable formulation. Under the hypothesis that the granular formulation may require more water for activation, an experiment was conducted to evaluate the interaction of flumioxazin formulation, rate, and activation moisture for PRE control of spotted spurge in a pine-bark substrate. Experiment consisted of a factorial arrangement of four experimental variables; flumioxazin formulation (granular and spray), and flumioxazin rate (0.28 and 0.42 kg ai ha−1), substrate moisture level at the time of application (dry, medium, and wet), and after-application irrigation level (a single irrigation at 0.6, 1.3, 2.5, and 5.1 cm). Treated pots were seeded with spotted spurge 2 d after the herbicide application, which was 1 d after the first irrigation. Control as indicated by spotted spurge counts and fresh weight was influenced only by flumioxazin rate and formulation. The sprayable formulation provided excellent control regardless of rate. Granular formulation was generally less effective, and additional activation moisture did not improve efficacy. Further studies were conducted with the granular formulation to determine the maximum separation distance between the spotted spurge seed and herbicide prills at which control is possible. Individual prills and spotted spurge seeds were placed on media surface at progressively increasing separation distances. Nonlinear regression of seedling survival data revealed that ≥99% control required a prill–seed separation of ≤5.2 mm. This prill–seed separation requirement is only marginally obtained with the current registered rate, i.e., 0.42 kg ai ha−1 or 168 kg product ha−1. The relationship between control and prill–seed separation distance cannot be manipulated by additional activation moisture. Inadequate contact between the spotted spurge seeds and the flumoioxazin-containing prills is likely the sole cause of inadequate control.

Selected Pacific Northwest Rangeland and Weed Plants as Hosts of Pratylenchus neglectus and P. thornei.

Eighteen rangeland plants and 16 weed species were assayed in the greenhouse for efficiency as hosts of Pratylenchus neglectus and P. thornei. Hosting ability ratings were assigned using the ratio of final versus initial nematode density and by comparing the final nematode density to that of susceptible wheat controls. Good hosts of both Pratylenchus spp. included thickspike bluegrass 'Critana', smooth brome 'Manchar', seven wheatgrasses, and jointed goatgrass. Good hosts of P. neglectus but not P. thornei included two hairy vetches, western wheatgrass 'Rosana', big bluegrass 'Sherman', tall wheatgrass 'Alkar', green foxtail, kochia, large crabgrass, palmer amaranth, redroot pigweed, tumble mustard, and wild oat. Good hosts of P. thornei but not P. neglectus included hard fescue 'Durar', sheep fescue 'Blacksheep', downy brome, and rattail fescue. Poor or minor hosts of both Pratylenchus spp. included two alfalfas, dandelion, horseweed, lambsquarters, prostrate spurge, and Russian thistle. These assays will provide guidance for transitioning rangeland into crop production and for understanding the role of weeds on densities of Pratylenchus spp. in wheat-production systems.

Erect and Prostrate Spurge Biology and Management in Turf

Erect and prostrate spurges are erect or upright annuals with branched stems. Leaves are opposite and not equal. Stems produce “milky sap.” A large number of spurge species occur in Florida. Reproduction occurs via seed. This 2-page fact sheet was written by J. Bryan Unruh, Darcy E. P. Telenko, Barry J. Brecke, and Ramon Leon, and published by the UF Department of Environmental Horticulture, December 2013.
 ENH1234/EP495: Erect and Prostrate Spurge Biology and Management in Turf (ufl.edu)

Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography-Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity.

The Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data. The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method. Correlation coefficients (r 2) were >0.9907. The limit of detection and limit of quantification of the method were >0.028 mg/L and 0.094 mg/L, respectively. Recoveries measured at 50 mg/L and 100 mg/L were 76.1–102.8% and 85.2–98.6%, respectively. The total amount of the identified polyphenols was 3352.9 ± 2.8 mg/kg fresh plant. Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols. The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase. Cell viability was effectively suppressed at polyphenol mixture concentrations >250 mg/L.

Evaluation of Container Ornamental Species Tolerance to Three p-hydroxyphenylpyruvate Dioxygenase-inhibiting Herbicides

Selective weed control in ornamental plant production can be difficult as many herbicides can cause unacceptable injury. Research was conducted to evaluate the tolerance of several ornamental species to applications of p-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides for the control of problematic weeds in ornamental production. Mestotrione (0.09, 0.18, and 0.36 lb/acre), tembotrione (0.08, 0.16, and 0.32 lb/acre), and topramezone (0.016, 0.032, and 0.064 lb/acre) were applied alone postemergence (POST) in comparison with the photosystem II-inhibiting herbicide, bentazon (0.5 lb/acre). All herbicide treatments, with the exception of the two highest rates of tembotrione, caused less than 8% injury to ‘Noble Upright’ japanese holly (Ilex crenata) and ‘Compactus’ burning bush (Euonymus alatus). Similarly, no herbicide treatment caused greater than 12% injury to ‘Girard’s Rose’ azalea (Azalea). Conversely, all herbicides injured flowering dogwood (Cornus florida) 10% to 23%. Mesotrione- and tembotrione-injured ‘Radrazz’ rose (Rosa) 18% to 55%, compared with only 5% to 18% with topramezone. ‘Siloam June Bug’ daylily (Hemerocallis) injury with topramezone and tembotrione was less than 10%. Topramezone was the only herbicide evaluated that provided at least 93% control of redroot pigweed (Amaranthus retroflexus) with all application rates by 4 weeks after treatment (WAT). Redroot pigweed was controlled 67% to 100% with mesotrione and tembotrione by 4 WAT, but this activity was variable among application rates. Spotted spurge (Chamaesyce maculata) was only adequately controlled by mesotrione applications at 0.18 and 0.36 lb/acre, whereas chamberbitter (Phyllanthus urinaria) was not controlled sufficiently with any herbicide evaluated in these studies. Yellow nutsedge (Cyperus esculentus) was suppressed 72% to 87% with mesotrione applications at 0.18 lb/acre or higher and with bentazon at 0.5 lb/acre by 4 WAT. All other herbicide treatments provided less than 58% control of yellow nutsedge. In the second study, ‘Patriot’ hosta (Hosta), ‘Green Sheen’ pachysandra (Pachysandra terminalis), autumn fern (Dryopteris erythrosora), ‘Little Princess’ spirea (Spiraea japonica), ‘Green Giant’ arborvitae (Thuja plicata), and ‘Rosea’ weigela (Weigela florida) displayed no response to topramezone when applied at 0.024 and 0.095 lb/acre. Since 10 ornamental species in our studies exhibited less than 10% herbicidal response with all rates of at least one HPPD-inhibiting herbicide then it is possible that these herbicides may provide selective POST weed control in ornamental production systems.

Differential dispersal of Chamaesyce maculata seeds by two ant species in Japan

Seed dispersal by ants (myrmecochory) is a widely distributed plant–animal interaction in many ecosystems, and it has been regarded as a generalized (multiple species) interaction in which specialization on specific ant partners is uncommon. In this paper, we demonstrate species-specific seed dispersal of spotted spurge (Chamaesyce maculata) by ants in Japan. C. maculata produces seeds from summer to autumn in Japan. The seeds produced in autumn are carried by two ant species, Tetramorium tsushimae and Pheidole noda. We performed laboratory experiments to investigate the fate of C. maculata seeds in the nests of T. tsushimae and P. noda. P. noda consumed the seeds in the nest and rarely carried seeds out of the nest, while T. tsushimae consumed only the seed coat, and subsequently carried the seeds out of the nest. Removal of the seed coat by T. tsushimae may increase seed survival by reducing their susceptibility to infection by fungi. We also observed ant responses to filter paper soaked with an aqueous extract of the seed coat. P. noda did not react to the filter paper, but T. tsushimae carried the filter paper into their nest. Analysis by high-pressure liquid chromatography revealed that the aqueous extract contained at least four sugars and one unknown substance. Myrmecochory has been regarded as a generalized interaction with specialization for specific ant partners uncommon. However, our study suggests there is a species-specific interaction in seed dispersal by ants in autumn-flowering individuals of C. maculata in Japan.

Early Postemergence Control of Spotted Spurge in Container Production

Abstract Four experiments were conducted to evaluate early postemergence control of spotted spurge (Chamaesyce maculata) in nursery crops using preemergence active herbicides. In Experiment 1, spotted spurge (SS) seed were overseeded at two different dates in a commercial pine bark substrate and were grown until reaching either the cotyledon to one leaf (C–1L) stage, or the two to four leaf (2–4L) stage. Herbicides applied included: Broadstar 1604 (flumioxazin), V-10142 (imazosulfuron), and Tower (dimethenamid-P). These products were applied postemergence at their respective recommended label rate (1 ×) and twice (2 ×) this rate to SS either at the C–1L stage or at the 2–4L stage. In general, V10142 and Tower provided the greatest injury to SS in the C–1L. Tower also provided greatest SS injury in the 2–4L stage. In Experiment 2, postemergence control was tested in the C1-L and 2–4L stages with Broadstar 1604, FreeHand (dimethenamid-P 0.75% + pendimethalin 1.0%), Tower, and Pendulum 3.3 EC (pendimethalin) at the 1× and 2× the label rate. FreeHand at 2×, along with Tower and Pendulum at both rates provided the highest SS injury ratings in the C–1L. Tower at 2× and Pendulum at both rates provided greatest SS injury in the 2–4L. In Experiments 3 and 4, Broadstar (1/2×, 1×, and 2× rate), Casoron (dichlobenil) (1× and 2×), HGH-63 (oxyflurfen) (1×), Certainty (sulfosulfuron) (1× and 2×), Tower (2/3× and 1×), V-10142 (1× and 2×), Pendulum 3.3 EC (1× and 2×), FreeHand (1× and 2×) were applied to SS in either C–1L or 2–4L as described above. Results from Experiments 3 and 4 indicate that Certainty (both rates), Tower (both rates), Pendulum (both rates), and FreeHand (both rates) have postemergence activity on immature SS, especially when applied in the C–1L stage. Postemergence activity declined when herbicides were applied to SS in the 2–4L, however Pendulum (both rates), Certainty (both rates), and FreeHand (2× rate) had a marginal effect.

Seed predator deterrence by seed‐carrying ants in a dyszoochorous plant, Chamaesyce maculata L. Small (Euphorbiaceae)

Seeds are often carried by omnivorous ants even if they do not carry elaiosomes. Although many seeds carried by ants are consumed, both seeds abandoned during the seed carrying and leftover seeds are consequently dispersed (dyszoochory). These non-myrmecochorous seeds do not necessarily attract ants quickly. Therefore, these seeds often seem to be exposed to the danger of consumption by pre-dispersal seed predators. We propose the hypotheses, “seed predator deterrence hypothesis” that plants may benefit from seed-carrying ants if they deter seed predators from visiting plants, and seed-carrying ants may play additional roles in plant reproductive success, besides dyszoochory by ants. To test the hypotheses, we investigated the abundance of seed-carrying ants of the species Tetramorium tsushimae Linnaeus and Pheidole noda Smith F., and of the seed predatory stinkbug, Nysius plebeius Distat, on the spotted sandmat, Chamaesyce maculata L. Small, of which the seeds have no elaiosomes but are consumed by both ants and bugs. In the field, ants and stinkbugs seldom encountered each other on the plant. The number of stinkbugs beneath the plants with ants was smaller than that beneath the plants without ants. In laboratory experiments, the number of stinkbugs on the shoot was smaller when ants were present than when they were absent. These results might support the seed predator deterrence hypothesis: the probability of seed predation by stinkbugs seems to be reduced by the ant visits on plants and/or the existence of ants beneath the plants. This study highlights a new ant–plant interaction in seed dispersal by ants.

Interaction of Glyphosate and Pelargonic Acid in Ready-to-Use Weed Control Products

Glyphosate-based, ready-to-use weed control products often contain pelargonic acid (PA) at a concentration equivalent to that of the glyphosate. It remains unclear what benefit, if any, this combination provides. Greenhouse experiments using large crabgrass, yellow nutsedge, longstalked phyllanthus, and prostrate spurge were conducted to determine whether the addition of PA improved weed control efficacy compared to glyphosate alone. Glyphosate was applied at a series of rates, ranging from 0.11 to 1.12 kg ae/ha, either alone or with an equal rate of PA. Addition of PA to glyphosate was synergistic only in longstalked phyllanthus and yellow nutsedge, and this synergism was manifested only as an increase in the amount of early (i.e., 5 to 7 d after treatment) visual injury. Conversely, longer-term control and control of regrowth was either not affected or reduced by the addition of PA. We conclude that the addition of PA to glyphosate in ready-to-use weed control products is neither warranted nor justified. However, we also note that the increase in early injury that was observed in only two of the four species evaluated could be an important attribute for the consumers for which these products are targeted.