You have accessJournal of UrologyTechnology & Instruments: Surgical Education & Skills Assessment1 Apr 20101345 AUTOFLUORESCENCE MICROSCOPY OF PERIPROSTATIC LYMPHATIC TISSUE AND CORRELATION WITH CONVENTIONAL HISTOPATHOLOGY IMAGING Ashutosh Tewari, Mike Herman, Sushmita Mukherjee, Rajiv Yadav, Sonal Grover, Kumaran Mudaliar, Youssef El Douaihy, Fred Maxfield, Mark Rubin, Abhishek Srivastava, David Peters, Warren R. Zipfel, DaWei Ye, Gerald Tan, Robert Leung, Josh Sterling, and Watt W. Webb Ashutosh TewariAshutosh Tewari More articles by this author , Mike HermanMike Herman More articles by this author , Sushmita MukherjeeSushmita Mukherjee More articles by this author , Rajiv YadavRajiv Yadav More articles by this author , Sonal GroverSonal Grover More articles by this author , Kumaran MudaliarKumaran Mudaliar More articles by this author , Youssef El DouaihyYoussef El Douaihy More articles by this author , Fred MaxfieldFred Maxfield More articles by this author , Mark RubinMark Rubin More articles by this author , Abhishek SrivastavaAbhishek Srivastava More articles by this author , David PetersDavid Peters More articles by this author , Warren R. ZipfelWarren R. Zipfel More articles by this author , DaWei YeDaWei Ye More articles by this author , Gerald TanGerald Tan More articles by this author , Robert LeungRobert Leung More articles by this author , Josh SterlingJosh Sterling More articles by this author , and Watt W. WebbWatt W. Webb More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.951AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES This was a prospective study involving imaging and histopathological correlations of human prostate and extra-prostatic lymphatic tissue to validate our hypothesis that laser imaging (Robo-Microscopy Project) is a minimally invasive imaging modality that provides both cellular and extracellular structural information with subcellular resolution and can be used for intraoperative imaging in future METHODS We imaged a palpable lymph node that had been removed with the prostate. The imaging was carried out using intrinsic fluorescence and scattering properties of the tissues without any exogenous dye or contrast agent (Robo-Microscopy-Project). A custom-built femtosecond pulsed titanium/sapphire laser at 780-nm wavelength was used to excite the tissue; laser power under the objective was modulated via a Pockels cell. Signals were collected at 390 (+/-35 nm), and broadband autofluorescence was collected at 380 to 530 nm. In certain cases, longer wavelength autofluorescence was collected at 530-650 nm. The images obtained from tissue fluorescence were then merged and color coded during post processing for better appreciation of details. The corresponding tissues were subjected to hematoxylin and eosin staining for histological confirmation of the structures. RESULTS We identified some non-glandular structures within the prostate. These structures are visually distinct from arteries, and veins; close examination of the diagnostic pathology suggests that these may in fact be lymphovascular spaces (fig 1). In Robo-Microscopy Project image of the dissected lymph nodes we observed an interconnecting network of autofluorescent vessels surrounding clusters of what appeared to be cells. Based on the appearance of the lymphovascular spaces we observed in the prostatic slices, we believe this network of autofluorescent vessels represents the lymph channels within the node. The H&E pathology of the same pelvic lymph node demonstrates collagenous septa; very little SHG signal was apparent, however. CONCLUSIONS Robo-Microscopy Project imaging has a great potential for application in the in vivo real time intraoperative imaging of the tissues, and may alleviate the need for intraoperative frozen sections in future. New York, NY© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e519-e520 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ashutosh Tewari More articles by this author Mike Herman More articles by this author Sushmita Mukherjee More articles by this author Rajiv Yadav More articles by this author Sonal Grover More articles by this author Kumaran Mudaliar More articles by this author Youssef El Douaihy More articles by this author Fred Maxfield More articles by this author Mark Rubin More articles by this author Abhishek Srivastava More articles by this author David Peters More articles by this author Warren R. Zipfel More articles by this author DaWei Ye More articles by this author Gerald Tan More articles by this author Robert Leung More articles by this author Josh Sterling More articles by this author Watt W. 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