AB263. Ideas from study on pathological large slices of prostate

Abstract

BackgroundPathological large slice is a new technology which is very applicable to diagnosis and research on prostate disorder. Here is a summary of experiences and ideas from learning on this technique in pathological department of Beijing Xiehe Hospital.MethodsThe protocol for pathological large slice manufacture of prostate was completely followed. Ask Xingqi Li, a leader of group on pathological technology in Beijing city for help with questions or problems on critical step such as fixation, dehydration and staining and make a discussion with him.ResultsTissue preparation is the first step for large slice technology, it is very important to record cutting edge and 3d position of each tissue block by combining marker and photo picture technique. So that it can be diagnosed correctly. Further, the tissue block must be evenly distributed with a thickness of 5mm or so. Thus large slice can be made conveniently. Dehydration is the second most critical process for large slice manufacture. Due to additional function on temperature and pressure control can promote liquid exchange process, Optimized automatic dehydration procedure can significantly improve the efficiency and obtain better results compared to manually dehydration. Slicing is the central process for large slice technique, some small tips and special techniques are required for proper slice cutting and extension. Further, a good equipment is very helpful for slice cutting. Yamato Kohki sliding cutting machine is one of the best choices because of its extra long knife rest with flexible angle adjusting and longer distance of cutting and specimen holder. Staining and slide sealing are also important and need some flexible adaption on the basis of regular procedure. Special care should be given to prevent bubble to avoid negative impact on diagnostic visual field.ConclusionsPathological large slide technology is a systematic project. Automatic dehydration machine procedure can obtain better results and greatly improve the efficiency for large slices manipulation of prostate.