Propulsive Motility Research Articles

Wheelchair caster power losses due to rolling resistance on sports surfaces

The gross mechanical efficiency of the manual wheelchair propulsion movement is particularly low compared to other movements. The energy losses in the manual wheelchair propulsion movement are partly due to energy losses associated with the wheelchair, and especially to the rolling resistance of the wheels. The distribution of mass between the front rear wheels and the caster wheels has a significant impact on the rolling resistance. The study of the caster wheels cannot therefore be neglected due to their involvement in rolling resistance. Thus, this study aimed to evaluate the power dissipated due to rolling resistance by different caster wheels, at different speeds and under different loadings on various terrains. Four caster wheels of different shapes, diameters, and materials were tested on two surfaces representative of indoor sports surfaces at four different speeds and under four loadings. The results showed a minimal dissipated power of 0.4 ± 0.2 W for the skate caster, on the parquet, at 0.5 m/s and under a loading of 50 N. The maximal mean power dissipated was 43.3 ± 27.6 W still for the skate caster, but on the Taraflex, at 1.5 m/s and under loading of 200 N. The power dissipated on the parquet was lower than the one on the Taraflex. The Spherical and Omniwheel caster wheels dissipated less power than the two other casters. This study showed that caster wheels cannot be neglected in the assessment of gross mechanical efficiency, particularly in light of the power dissipated by athletes during propulsion.

Changing the Mandibular Position in Rowing: A Brief Report of a World-Class Rower.

We investigated the acute biophysical responses of changing the mandibular position during a rowing incremental protocol. A World-class 37-year-old male rower performed two 7 × 3 min ergometer rowing trials, once with no intraoral splint (control) and the other with a mandibular forward repositioning splint (splint condition). Ventilatory, kinematics and body electromyography were evaluated and compared between trials (paired samples t-test, p ≤ 0.05). Under the splint condition, oxygen uptake was lower, particularly at higher exercise intensities (67.3 ± 2.3 vs. 70.9 ± 1.5 mL·kg-1·min-1), and ventilation increased during specific rowing protocol steps (1st-4th and 6th). Wearing the splint condition led to changes in rowing technique, including a slower rowing frequency ([18-30] vs. [19-32] cycles·min-1) and a longer propulsive movement ([1.58-1.52] vs. [1.56-1.50] m) than the control condition. The splint condition also had a faster propulsive phase and a prolonged recovery period than the control condition. The splint reduced peak and mean upper body muscle activation, contrasting with an increase in lower body muscle activity, and generated an energetic benefit by reducing exercise cost and increasing rowing economy compared to the control condition. Changing the mandibular position benefited a World-class rower, supporting the potential of wearing an intraoral splint in high-level sports, particularly in rowing.

Secretin Inhibits Small Intestinal Motility via Interstitial Cells of Cajal and cAMP Mechanisms

Background: Secretin is a member of the secretin-glucagon-vasoactive intestinal peptide hormone superfamily and is a multifunctional gastrointestinal- (GI) and neuro- peptide hormone. Secretin is primarily secreted postprandially from the crypts of Lieberkühn of duodenal enteroendocrine S cells into circulation where it reaches secretin receptor targets in the central nervous system and periphery. This dynamic hormone has been shown to act as a key signaling molecule in the regulation of digestion, metabolism and energy expenditure, water retention, reproduction, thermogenesis in adipose tissue, and in gastric and intestinal motility. Secretin’s canonical role in the GI tract is to stimulate the secretion of bicarbonate and bile from pancreatic ducts and bile ducts to neutralize acidic chyme exiting the stomach. Secretin has also been shown to slow intestinal motility, but its targets and mechanism of action is poorly understood. Aims: Several studies have proposed that secretin acts to slow intestinal motility in the intestines primarily through the many secretin receptors present on vagal afferents in the GI tract, here we discuss new data that suggests an alternate and complementary signaling pathway via interstitial cells of Cajal (ICC). ICC act as a liaison to facilitate a reduction in force of GI smooth muscle contraction through the activation of the secretin receptor (SCTR) and subsequent stimulation of the second messenger, cyclic adenosine monophosphate (cAMP). Here we provide evidence to show how ICC mediates changes in myogenic activity and motility in the small intestine. Methods: Spinning-disk confocal microscopy was used to monitor Ca2+ signaling in ICC from small intestinal muscles of GCaMP6f x KitiCre mice. Additionally, cAMP levels were evaluated using CAMPER mice. Intestinal muscle contractility was assessed using muscle strip myography experiments. Results: Secretin reduced small intestinal force of contraction in the presence of tetrodotoxin (TTX) and dampened the effect of cholinergic transmission. The secretin receptor (SCTR) is expressed primarily on ICC, specifically ICC within the deep muscular plexus (ICC-DMP) in the small intestine and Ca2+ imaging confirmed the effects are primarily localized within ICC-DMP. Secretin reduced carbachol-induced contractions and Ca2+ transients in ICC-DMP in response to electrical field stimulation (EFS) in the presence of LNNA (NO synthase inhibitor) and MRS2500 (P2Y1 antagonist). Secretin caused an increase in cAMP levels in ICC-DMP in muscles from Kit-iCre-CAMPER mice. PKA inhibitors rescued some of the effects of secretin on ICC-DMP Ca2+ signaling. Measurements of diameter change in large, intact, intestinal segments (4-5 cm) showed a significant decrease in response to Secretin. Conclusions: Secretin can inhibit small intestinal motility through the activation SCTRs on ICC-DMP via cAMP-mediated mechanisms. These results show how novel secretin targets on ICC influence GI muscles and reduce propulsive and segmental motility to facilitate nutrient absorption and digestion after a meal. Funding: This project was supported by R01 DK-120759 from the National Institute of Diabetes and digestive and Kidney (NIDDK). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Computational insights into colonic motility: Mechanical role of mucus in homeostasis and inflammation

Colonic motility plays a vital role in maintaining proper digestive function. The rhythmic contractions and relaxations facilitate various types of motor functions that generate both propulsive and non-propulsive motility modes which in turn generate shear stresses on the epithelial surface. However, the interplay between colonic mucus, shear stress, and epithelium remains poorly characterized. Here, we present a colonic computational model that describes the potential roles of mucus and shear stress in both homeostasis and ulcerative colitis (UC). Our model integrates several key features, including the properties of the mucus bilayer and faeces, intraluminal pressure, and crypt characteristics to predict the time-space mosaic of shear stress. We show that the mucus thickness which could vary based on the severity of UC, may significantly reduce the amount of shear stress applied to the colonic crypts and effect faecal velocity. Our model also reveals an important spatial shear stress variance in homeostatic colonic crypts that suggests shear stress may have a modulatory role in epithelial cell migration, differentiation, apoptosis, and immune surveillance. Together, our study uncovers the rather neglected roles of mucus and shear stress in intestinal cellular processes during homeostasis and inflammation.

Study on Oscillatory and Undulatory Motion of Robotic Fish

This paper conducts a comprehensive analysis of undulating and oscillatory movements in fish, utilizing numerical simulations to explore correlations among fin thrust and swimming speed. The study distinguishes itself through a unique approach by employing kinematic equations of motion control, specifically in oscillation and undulation, for computational fluid dynamics. Despite increasing energy loss with undulation, the study reveals a reduction in power demand with oscillation, underscoring its effectiveness in achieving desired speeds. The dynamics of undulating fins in aquatic and aerial locomotion remain insufficiently understood. The trade-off between more energy-consuming but highly propulsive movements or simpler and faster movements requires sophisticated design techniques to reduce volume. The geometry, developed using Rhino 6 software, incorporates precise fluid resistance calculations conducted with Ansys Fluent 19. Spanning flow velocities from 1 to 4 m/s were used for the simulation condition. Critical factors such as flexibility, viscosity, and shape change were meticulously examined for their impact on efficiency enhancement.

Microbiota-derived acetylcholine can promote gut motility in Drosophila melanogaster.

The gut microbiota is crucial for intestinal health, including gastrointestinal (GI) motility. How commensal bacterial species influence GI motility has not been fully elucidated. A major factor of GI motility is the gut contraction promoting the propulsive movement of orally ingested materials. Here, we developed a method to monitor and quantify gut contractions in living Drosophila melanogaster larvae. We found that the culture medium of an isolated strain Lactiplantibacillus plantarum Lsi promoted gut contraction in vivo, which was not observed in Leuconostoc sp. Leui nor Acetobacter persici Ai culture medium. To identify bacteria-derived metabolites, we performed metabolome analysis of the culture media by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Of the 66 metabolites detected, we found that some metabolites changed in a species-specific manner. Among them, acetylcholine was specifically produced by L. plantarum. Feeding exogenous acetylcholine increased the frequency of gut contractions, which was blocked by D-tubocurarine, an inhibitor of nicotinic acetylcholine receptors. In this study, we propose a mechanism by which the gut microbiota influences Drosophila gut motility. This article is part of the theme issue 'Sculpting the microbiome: how host factors determine and respond to microbial colonization'.

Short Communication - Possible aetiology of the posterior presentation in Perosomus elumbis

The aetiology of foetal presentation in cattle is still unresolved. The hind legs are the main source of propulsive movement in cattle, which provides changes in foetal presentation. The assumption is that in the absence of hind leg movements of the foetus, as in the case of the congenital malformation, Perosomus elumbis, the incidence of the anterior and posterior presentation will be the same. The Scopus database, the world wide web (www), and the list of article references were searched using the keyword, Perosomus elumbis. Manuscripts with data about the foetal presentation were included and 21 cases in 16 articles were identified. There were 11 cases of anterior presentation and 10 of posterior presentation. The Chi-square test didn't show a statistically significant difference between the incidence of anterior and posterior presentation. This finding supports the assumption that the posterior presentation is the result of the random occupying of the intrauterine space with the same probability of anterior and posterior presentation. To confirm or exclude at random the occupant of the intrauterine space with the same probability for anterior and posterior presentation as a general mechanism for posterior presentation in cattle foetuses, it is necessary to determine the incidence of foetal presentation at birth in various veterinary entities accompanied by an increased incidence of posterior presentation. The aim of the paper was to investigate the incidence of posterior and anterior presentation in Perosomus elumbis.

Chronic intestinal pseudo-obstruction: associations with gut microbiota and genes expression of intestinal serotonergic pathway

BackgroundPediatric chronic intestinal pseudo-obstruction (PIPO) is a rare disease characterized by symptoms and radiological signs suggestive of intestinal obstruction, in the absence of lumen-occluding lesions. It results from an extremely severe impairment of propulsive motility. The intestinal endocrine system (IES) jointly with the enteric nervous system (ENS) regulates secreto-motor functions via different hormones and bioactive messengers/neurotransmitters. The neurotransmitter 5-hydroxytryptamine (5-HT) (or serotonin) is linked to intestinal peristalsis and secretory reflexes. Gut microbiota and its interplay with ENS affect 5-HT synthesis, release, and the subsequent serotonin receptor activation. To date, the interplay between 5-HT and gut microbiota in PIPO remains largely unclear. This study aimed to assess correlations between mucosa associated microbiota (MAM), intestinal serotonin-related genes expression in PIPO. To this purpose, biopsies of the colon, ileum and duodenum have been collected from 7 PIPO patients, and 7 age-/sex-matched healthy controls. After DNA extraction, the MAM was assessed by next generation sequencing (NGS) of the V3-V4 region of the bacterial RNA 16 S, on an Illumina Miseq platform. The expression of genes implicated in serotoninergic pathway (TPH1, SLC6A4, 5-HTR3 and 5-HTR4) was established by qPCR, and correlations with MAM and clinical parameters of PIPO have been evaluated.ResultsOur results revealed that PIPO patients exhibit a MAM with a different composition and with dysbiosis, i.e. with a lower biodiversity and fewer less connected species with a greater number of non-synergistic relationships, compared to controls. qPCR results revealed modifications in the expression of serotonin-related intestinal genes in PIPO patients, when compared to controls. Correlation analysis do not reveal any kind of connection.ConclusionsFor the first time, we report in PIPO patients a specific MAM associated to underlying pathology and an altered intestinal serotonin pathway. A possible dysfunction of the serotonin pathway, possibly related to or triggered by an altered microbiota, may contribute to dysmotility in PIPO patients. The results of our pilot study provide the basis for new biomarkers and innovative therapies targeting the microbiota or serotonin pathways in PIPO patients.

Sites and mechanisms of action of colokinetics at dopamine, ghrelin and serotonin receptors in the rodent lumbosacral defecation centre.

Agonists of dopamine D2 receptors (D2R), 5-hydroxytryptamine (5-HT, serotonin) receptors (5-HTR) and ghrelin receptors (GHSR) activate neurons in the lumbosacral defecation centre, and act as 'colokinetics', leading to increased propulsive colonic motility, in vivo. In the present study, we investigated which neurons in the lumbosacral defecation centre express the receptors and whether dopamine, serotonin and ghrelin receptor agonists act on the same lumbosacral preganglionic neurons (PGNs). We used whole cell electrophysiology to record responses from neurons in the lumbosacral defecation centre, following colokinetic application, and investigated their expression profiles and the chemistries of their neural inputs. Fluorescence in situ hybridisation revealed Drd2, Ghsr and Htr2C transcripts were colocalised in lumbosacral PGNs of mice, and immunohistochemistry showed that these neurons have closely associated tyrosine hydroxylase and 5-HT boutons. Previous studies showed that they do not receive ghrelin inputs. Whole cell electrophysiology in adult mice spinal cord revealed that dopamine, serotonin, α-methylserotonin and capromorelin each caused inward, excitatory currents in overlapping populations of lumbosacral PGNs. Furthermore, dopamine caused increased frequency of both IPSCs and EPSCs in a cohort of D2R neurons. Tetrodotoxin blocked the IPSCs and EPSCs, revealing a post-synaptic excitatory action of dopamine. In lumbosacral PGNs of postnatal day 7-14 rats, only dopamine's postsynaptic effects were observed. Furthermore, inward, excitatory currents evoked by dopamine were reduced by the GHSR antagonist, YIL781. We conclude that lumbosacral PGNs are the site where the action of endogenous ligands of D2R and 5-HT2R converge, and that GHSR act as a cis-modulator of D2R expressed by the same neurons. KEY POINTS: Dopamine, 5-hydroxytryptamine (5-HT, serotonin) and ghrelin (GHSR) receptor agonists increase colorectal motility and have been postulated to act at receptors on parasympathetic preganglionic neurons (PGNs) in the lumbosacral spinal cord. We aimed to determine which neurons in the lumbosacral spinal cord express dopamine, serotonin and GHSR receptors, their neural inputs, and whether agonists at these receptors excite them. We show that dopamine, serotonin and ghrelin receptor transcripts are contained in the same PGNs and that these neurons have closely associated tyrosine hydroxylase and serotonin boutons. Whole cell electrophysiology revealed that dopamine, serotonin and GHSR receptor agonists induce an inward excitatory current in overlapping populations of lumbosacral PGNs. Dopamine-induced excitation was reversed by GHSR antagonism. The present study demonstrates that lumbosacral PGNs are the site at which actions of endogenous ligands of dopamine D2 receptors and 5-HT type 2 receptors converge. Ghrelin receptors are functional, but their role appears to be as modulators of dopamine effects at D2 receptors.

Postoperative ileus in abdominal surgery: in search for a solution to a current problem

In abdominal surgery, after operations on the abdominal cavity, it is possible to develop disturbances in the normal coordinated propulsive motility of the gastrointestinal tract. This episode of gastrointestinal hypomotility or the state of dynamic ileus is referred to as postoperative ileus (POI) or postoperative gastrointestinal tract paresis. The incidence of POI in the clinic of abdominal surgery ranges from 10 to 30%. Chewing gum has been used in surgery to relieve postoperative intestinal obstruction since the early 21st century. The present review considers the main randomized clinical trials, reviews and meta-analyses on the study of the effect of chewing gum in abdominal surgery for the prevention of postoperative ileus. The data presented in the review indicate the effectiveness and safety of the use of chewing gum in the postoperative period for the prevention of POI in abdominal surgery and surgical interventions in related areas.

Current problem - postoperative ileus

In abdominal surgery, after operations on the abdominal cavity, it is possible to develop disturbances in the normal coordinated propulsive motility of the gastrointestinal tract. This episode of gastrointestinal hypomotility or the state of dynamic ileus is referred to as postoperative ileus (POI) or postoperative gastrointestinal tract paresis. The incidence of POI in the clinic of abdominal surgery ranges from 10 to 30%. Chewing gum has been used in surgery to relieve postoperative intestinal obstruction since the early 21st century. The present review considers the main randomized clinical trials, reviews and meta-analyses on the study of the effect of chewing gum in abdominal surgery for the prevention of postoperative ileus. The data presented in the review indicate the effectiveness and safety of the use of chewing gum in the postoperative period for the prevention of POI in abdominal surgery and surgical interventions in related areas.

Перспективное решение проблемы послеоперационного илеуса в урологии и травматологии

In surgical practice, after operations that directly or indirectly affect the organs of the abdominal cavity, it is possible to develop disturbances in the normal coordinated propulsive motility of the gastrointestinal tract. This condition of dynamic intestinal obstruction is referred to as postoperative gastrointestinal paresis or postoperative ileus (POI). We did not find any information on the frequency of POI in urological practice, however, the frequency of POI in the clinic of colorectal surgery is known to be from 10 to 30% and after gynecological operations 10-15%. The issue of treatment and prevention of POI remains relevant. In global clinical practice, chewing gum has been used after surgery to reduce postoperative intestinal obstruction since the beginning of the 21st century. 141 references were found for the query “postoperative ileus, chewing gum” in the PubMed database alone, of which 53 references to randomized clinical trials, 48 to reviews and systematic reviews, and 26 to meta-analyses. The present review considers randomized clinical trials, systematic reviews and meta-analyzes that examined the prevention of POI in urology and traumatology, indicating the effectiveness and safety of the use of chewing gum in the postoperative period for the prevention of postoperative ileus.

Comparison of the arm-stroke kinematics between maximal and sub-maximal breaststroke swimming using discrete data and time series analysis

The aim of the current study was to compare the arm-stroke kinematics during maximal and sub-maximal breaststroke swimming using both discrete and continuous data analysis. Nine male breaststrokers swam 2 × 25 m with maximal and sub-maximal intensity and their full body 3-D kinematics were obtained using eight video cameras. The arm-stroke was divided into five phases: recovery, glide, out-sweep, in & down-sweep and in & up-sweep. The statistical treatment of selected discrete variables was conducted using t-test, while the analysis of their equivalent time series, when applicable, was conducted using Statistical Parametric Mapping. Sub-maximal trial, compared to maximal, presented lower swimming velocity, greater stroke length and less stroke rate. Moreover, the absolute and relative duration of the glide phase was longer, while the relative duration of all the other phases was shorter. The resultant hand velocity during the arm recovery was slower, as well as the hand velocity time series in the transverse and longitudinal axis which were slower from ∼ 45 % to ∼ 60 % and from ∼ 5 % to ∼ 15 % of the stroke cycle, respectively. Both discrete and continuous data analysis revealed that the main discriminating factor between the two conditions concerns to the adjustment of the glide and the recovery phase and consequently the continuation of the propulsive movements.

5xFAD mice do not have myenteric amyloidosis, dysregulation of neuromuscular transmission or gastrointestinal dysmotility.

Alterations in gastrointestinal (GI) function and the gut-brain axis are associated with progression and pathology of Alzheimer's Disease (AD). Studies in AD animal models show that changes in the gut microbiome and inflammatory markers can contribute to AD development in the central nervous system (CNS). Amyloid-beta (Aβ) accumulation is a major AD pathology causing synaptic dysfunction and neuronal death. Current knowledge of the pathophysiology of AD in enteric neurons is limited, and whether Aβ accumulation directly disrupts enteric neuron function is unknown. In 6-month-old 5xFAD (transgenic AD) and wildtype (WT) male and female mice, GI function was assessed by colonic transit in vivo; propulsive motility and GI smooth muscle contractions ex vivo; electrochemical detection of enteric nitric oxide release in vitro, and changes in myenteric neuromuscular transmission using smooth muscle intracellular recordings. Expression of Aβ in the brain and colonic myenteric plexus in these mice was determined by immunohistochemistry staining and ELISA assay. At 6months, 5xFAD mice did not show significant changes in GI motility or synaptic neurotransmission in the small intestine or colon. 5xFAD mice, but not WT mice, showed abundant Aβ accumulation in the brain. Aβ accumulation was undetectable in the colonic myenteric plexus of 5xFAD mice. 5xFAD AD mice are not a robust model to study amyloidosis in the gut as these mice do not mimic myenteric neuronal dysfunction in AD patients with GI dysmotility. An AD animal model with enteric amyloidosis is required for further study.

Extraction and Fractionation of Prokinetic Phytochemicals from Chrozophora tinctoria and Their Bioactivities.

Chrozophora tinctoria is an annual plant of the family Euphorbiaceae, traditionally used as a laxative, a cathartic and an emetic. A methanolic extract of Chrozophora tinctoria (MEC) whole plant and an n-butanol fraction of Chrozophora tinctoria (NBFC) were analyzed by gas chromatography–mass spectrometry (GC-MS) to detect the phytochemicals. MEC and NBFC were tested for in vitro anti acetylcholinesterase (AChE) potential. The effect of both samples on intestinal propulsive movement and spasmolytic activity in the gastrointestinal tract (GIT) was also studied. About twelve compounds in MEC and three compounds in NBFC were tentatively identified through GC-MS. Some of them are compounds with known therapeutic activity, such as toluene; imipramine; undecane; 14-methyl-pentadecanoic acid methyl ester; and hexadecanoic acid. Both NBFC and MEC samples were checked for acute toxicity and were found to be highly toxic in a dose-dependent manner, causing diarrhea and emesis at 1 g/kg concentration in pigeons, with the highest lethargy and mortality above 3 g/kg. Both the samples of Chrozophora tinctoria revealed significant (p ≤ 0.01) laxative activity against metronidazole (7 mg/kg) and loperamide hydrochloride (4 mg/kg)-induced constipation. NBFC (81.18 ± 2.5%) and MEC (68.28 ± 2.4%) significantly increased charcoal meal intestinal transit compared to distal water (41.15 ± 4.3%). NBFC exhibited a significant relaxant effect (EC50 = 3.40 ± 0.20 mg/mL) in spontaneous rabbit jejunum as compared to MEC (EC50 = 4.34 ± 0.68 mg/kg). Similarly, the impact of NBFC on KCl-induced contraction was more significant than that of MEC (EC50 values of 7.22 ± 0.06 mg/mL and 7.47 ± 0.57 mg/mL, respectively). The present study scientifically validates the folk use of Chrozophora tinctoria in the management of gastrointestinal diseases such as constipation. Further work is needed to isolate the phytochemicals that act as diarrheal agents in Chrozophora tinctoria.

Posture Estimation Using Surface Electromyography during Wheelchair Hand-Rim Operations.

This study examined competitive wheelchairs that facilitate sports participation. They can be moved straight ahead using only one arm. Our designed and developed competitive wheel-chairs have a dual hand-rim system. Their two hand-rims, attached to a drive wheel on one side, can be operated simultaneously for straight-ahead movement. Specifically, based on integrated electromyography (iEMG) data calculated from surface electromyography (sEMG), we examined the wheelchair loading characteristics, posture estimation, and effects on body posture during one-arm propulsion movement. The first experiment yielded insights into arm and shoulder-joint muscle activation from iEMG results obtained for two-hand propulsion and dual hand-rim system propulsion. Results suggest that muscle activation of one arm can produce equal propulsive force to that produced by two arms. The second experiment estimated the movement posture from iEMG during one-arm wheelchair propulsion. The external oblique abdominis is particularly important for one-arm wheelchair propulsion. The iEMG posture estimation validity was verified based on changes in the user body axis and seat pressure distribution. In conclusion, as confirmed by iEMG, which is useful to estimate posture during movement, one-arm wheelchair use requires different muscle activation sites and posture than when using two arms.

Prokinetic and Laxative Effects of Chrozophora tinctoria Whole Plant Extract.

Chrozophora tinctoria (Euphorbiaceae) has been used as an emetic, anthelminthic, and cathartic agent in traditional medicine. We used gas chromatography-mass spectrometry (GC-MS) to characterize the composition of ethyl acetate (EAC) and dichloromethane (DCMC) fractions from the whole Chrozophora tinctoria plant. EAC and DCMC fractions were evaluated for acetylcholinesterase (AChE) inhibitory activity and acute toxicity. Their effects on intestinal propulsive movement and spasmogenic activity of the gastrointestinal tract (GIT) muscle were also assessed. The compounds detected in both fractions were mostly fatty acids, with about seven compounds in EAC and 10 in DCMC. These included pharmacologically active compounds such as imipramine, used to treat depression, or hexadecanoic acid methyl ester, an antioxidant. Both EAC and DCMC fractions inhibited acetylcholinesterase (AChE) activity with IC50 values of 10 µg and 130 µg, respectively. Both the fractions were found to be toxic in a dose-dependent manner, inducing emesis at 0.5 g or higher and lethargy and mortality from 3–5 g upwards. Similarly, both of the fractions showed laxative activity in metronidazole- and loperamide-induced constipation models. EAC relaxed the intestinal muscle at a lower dose (1 mg/mL) than DCMC. Similarly, the EAC extract showed a significant relaxation effect (EC50 = 0.67 ± 0.15 mg/mL) on KCL-induced contraction in rabbit jejunum as compared to DCMC (EC50 = 5.04 ± 0.05 mg/kg). The present study strongly supports the folklore that this valuable plant is a cathartic agent. Further work is required to isolate and validate the bioactive compounds that act as diarrheal agents in Chrozophora tinctoria.

Helical micro-swimmer: hierarchical tail design and propulsive motility.

Helical micro-swimmers have markedly extended the reach of human beings in numerous fields, ranging from in vitro tasks in lab-on-a-chip to in vivo applications for minimally invasive medicine. The previous studies on the propulsive motility optimization of the micro-swimmers mainly focused on the distinct actuation principles (e.g., chemically powered, magnetic- or ultrasound energy-driven) and paid little attention to the structural design of these swimming machines themselves. The improvements of the structures can assist the externally powered motors in providing propulsion in a tiny scale and satisfy the agile locomotion demands. This paper presents the design, mechanics modeling and available experiments of a novel type of hierarchical helical swimming robot that significantly enhances the motility of the helix-based swimmers. Validated by the resistive force theory, our numerical model can well analyze the mechanical properties with a variety of geometric parameters. The motion performance of the hierarchical and conventional helical structures in low Reynolds regimes is presented, highlighting the advantages of hierarchical swimmers over the existing typical swimmers. In addition, the stability and resilience of the hierarchical swimmers can be maintained at a decent level. Moreover, the variable forward velocity resulting from the combined hierarchical structures is investigated here, which can thereby serve as a reliable design strategy. The proposed hierarchical helical design enables enticing opportunities for various device systems of medical robots and bio-integrated electronics.

Altered Glycemic Status Modulates Gastrointestinal Motility in Male Wistar Rats

Glucose derangement is a common endocrine disorder affecting over 463 million worldwide. Its attending complications on the gastrointestinal motility in altered glycemic states were investigated. Forty-five male Wistar rats were grouped into 3 (n=15/group): Group 1- Control, Group 2- Diabetes, Group 3- Postprandial hyperglycemia. The 15 rats per group were sub-grouped into 3 of 5 rats each. Each sub-group determined gastric emptying, intestinal propulsion, and colonic motility. Diabetes was induced with a single intraperitoneal 150 mg/kg alloxan and the postprandial received an oral D - glucose load of 2 g/kg. Before the commencement of each experimental procedure, rats in all groups fasted for 18 hours and blood glucose concentration was assessed by the glucose oxidase method after 45 min of onset of the experiment. The mean ± SEM of data were subjected to one-way ANOVA analysis and values of p<0.05 were considered significant. There was a significant decrease in the fasting blood glucose level (mmol/L) in rats of control (3.64 ± 0.10) compared with the diabetic (31.94 ± 2.38). The percentage of gastric emptying in the control decreased significantly compared with the diabetics. The percentage of intestinal propulsion in the control (42.14 ± 3.14) increased significantly compared with the diabetic (7.43 ± 1.05) and significantly decrease when compared with the postprandial (72.91 ± 5.47). There colonic motility time in the control decreased significantly compared with the diabetic. Diabetes promotes delay in the different motility examined while the postprandial group supported intestinal propulsion. Further work should focus on identifying the mechanism involved.

Numerical Simulator for Manual Wheelchair Propulsion based on a MPC Approach

Quite recently, considerable attention has been paid to the concept of prediction of manual wheelchair locomotion by a predictive model simulation, with the aim of facilitating the synthesis of the biomechanics of propulsion movement and improving the wheelchair ergonomics. Generally, the biomechanical modeling of wheelchair propulsion is a highly nonlinear that depends on the upper limb motion and the contact force between the human hand and the handrim, which will increase the complexity of implementing these models to evaluate with numerical simulation the biomechanics of propulsion. In this context, a new approach for the propulsion motion prediction of a manual wheelchair based on nonlinear model predictive control (NMPC) has been proposed. Based only on the kinematics of the hand on the handrim and the dynamics of the rear wheels, this approach is able to predict the optimal hand contact on the handrim, during the push phase and the optimal hand trajectory during the recovery phase. Thus, for a given speed configuration, the predictive model simulation is able to generate the most suitable propulsion scheme. The advantage of using a simple model of the hand-wheel interaction, could allow for an easier implementation while providing a fully predictive simulation of the wheelchair propulsion. Simulation results are presented, showing the effectiveness of the proposed approach.