In order to investigate if inter-limb propriospinal reflexes participate in coordination of locomotive movements of fore- and hindlimbs, we examined the relations between fore- and hindlimbs during overground locomotion of adult cats with spinal cord lesions. In a group of cats (T-T preparations), the spinal cord was hemisected first at around Th 12 and then at intervals of 37–126 days contralaterally at midthoracic level, propriospinal tracts being mostly severed in this group. In a second group of cats (C-T preparations), which received hemisections first at around C 2 and then at intervals of 21–73 days at mid-thoracic level, propriospinal tracts were left intact at least on one side of the spinal cord. Control observations were also made in intact cats and those with single hemisections at C 2 or Th 12, or with double unilateral hemisections at Th 6 and Th 12. Thus, it was found that in both T-T and C-T preparations, step length of the forelimbs was shortened significantly, whereas that of the hindlimbs was significantly lengthened. Furthermore, phase relations between the fore- and hindlimbs were completely lost in these preparations, suggesting that the stepping generator for the forelimbs operates independently of that for the hindlimbs. In other single-hemisected or unilaterally double-hemisected preparations, by contrast, no such changes were observed. The close similarity of the results in T-T and C-T preparations, in spite of different degrees of impairment of propriospinal tracts in them, leads to a conclusion that inter-limb propriospinal reflexes play little role in coordination of locomotive movements of fore- and hindlimbs.