Proportion Of C4 Plants Research Articles

Carbon isotope variations of modern soils in the eastern margin of the Tibetan Plateau and their controlling factors

The marginal areas of the Tibetan Plateau have great vertical altitude gradient and abundant vegetation, they are therefore the ideal places for investigating the relationships among carbon isotope composition (δ13C) of modern soils, vegetation and environmental factors, which would be very useful for the reconstructions of both paleovegetation and paleoclimate. In this paper, modern soil samples collected in different vegetation vertical zones along 4 km elevation gradient in the eastern margin of the Tibetan Plateau were analyzed for their carbon isotope composition. The results show that the modern soils in different vegetation vertical zones show apparent difference of δ13C values, which get heavier in the sequence of mixed evergreen and deciduous broad-leaved forest (−27.28‰ on average), evergreen broad-leaved forest (−27.25‰), subalpine shrub-meadow (−25.81‰), subalpine coniferous forest (−25.81‰), alpine bush-meadow (−25.16‰), and drought-enduring shrub (−24.07‰). 1800 m and 3500 m are two critical points for the δ13C values with respect to altitude. Specifically, the δ13C values decrease with increasing altitude below both points while increasing with increasing altitude above both points. Further analyses indicate that the declining δ13C values are mainly controlled by the decreasing proportion of C4 plants with elevation and the increasing δ13C values are attributed to the plant physic-morphological adaptation to the alpine environment. In the absence of drought stress, temperature is the main controlling factor for the carbon isotopic variations with altitude gradient.

Isotope analysis combined with DNA barcoding provide new insights into the dietary niche of khulan in the Mongolian Gobi.

With increasing livestock numbers, competition and avoidance are increasingly shaping resource availability for wild ungulates. Shifts in the dietary niche of wild ungulates are likely and can be expected to negatively affect their fitness. The Mongolian Gobi constitutes the largest remaining refuge for several threatened ungulates, but unprecedentedly high livestock numbers are sparking growing concerns over rangeland health and impacts on threatened ungulates like the Asiatic wild ass (khulan). Previous stable isotope analysis of khulan tail hair from the Dzungarian Gobi suggested that they graze in summer but switch to a poorer mixed C3 grass / C4 shrub diet in winter, most likely in reaction to local herders and their livestock. Here we attempt to validate these findings with a different methodology, DNA metabarcoding. Further, we extend the scope of the original study to the South Gobi Region, where we expect higher proportions of low-quality browse in the khulan winter diet due to a higher human and livestock presence. Barcoding confirmed the assumptions behind the seasonal diet change observed in the Dzungarian Gobi isotope data, and new isotope analysis revealed a strong seasonal pattern and higher C4 plant intake in the South Gobi Region, in line with our expectations. However, DNA barcoding revealed C4 domination of winter diet was due to C4 grasses (rather than shrubs) for the South Gobi Region. Slight climatic differences result in regional shifts in the occurrence of C3 and C4 grasses and shrubs, which do not allow for an isotopic separation along the grazer-browser continuum over the entire Gobi. Our findings do not allow us to confirm human impacts upon dietary preferences in khulan as we lack seasonal samples from the South Gobi Region. However, these data provide novel insight into khulan diet, raise new questions about plant availability versus preference, and provide a cautionary tale about indirect analysis methods if used in isolation or extrapolated to the landscape level. Good concordance between relative read abundance of C4 genera from barcoding and proportion of C4 plants from isotope analysis adds to a growing body of evidence that barcoding is a promising quantitative tool to understand resource partitioning in ungulates.

Host range determination in a novel outbreak pest of sugarcane, Cacosceles newmannii (Coleoptera: Cerambycidae, Prioninae), inferred from stable isotopes

AbstractAn outbreak of Cacosceles newmannii (Coleoptera: Cerambycidae) was detected for the first time on sugarcane (Saccharum spp.) in 2015 in KwaZulu‐Natal, South Africa. Although primary host plants of this native species remain unknown, these are central to testing hypotheses concerning the outbreak. We hypothesized that this species has undergone a host plant shift (i.e. a feeding association with a novel host plant). We compared δ13C and δ15N ratios of adult beetles retrieved from South African museum collections, collected between 1891 and 2016 (n = 23; ‘pre‐outbreak’), with samples from infested fields in 2017 (n = 9, ‘post‐outbreak’) and in 2019 (n = 23, ‘post‐outbreak’), as well as diverse, plausible host plants (n = 42 samples across 10 species) from infested fields and surrounding patches of indigenous and commercial forest vegetation. We used Bayesian isotope mixing models to infer the relative contribution of the different plants to the diet of C. newmannii. Pre‐outbreak, C3 plants contributed strongly to the larval diet, whereas post‐outbreak, C4 plants were the largest component of their diet. There was some indication of C4 plants contributing to their diet pre‐outbreak. Our results suggest that the outbreak of this polyphagous beetle was not a dramatic host shift but rather a rapid increase in the proportion of C4 plants already in their diet. We concluded that plants from the families Fabaceae and Poaceae are the most likely host plants of this species. Nevertheless, the drivers of this rapid outbreak on sugarcane remain poorly determined and should be the focus of future research.

Changes in vegetation type on the Chinese Loess Plateau since 75 ka related to East Asian Summer Monsoon variation

Floral communities in terrestrial habitats such as steppes are highly sensitive to regional climate changes. Here, we generated carbon and nitrogen isotope profiles for soil organic matter (SOM) in the Weinan loess-paleosol section in order to investigate changes in floral communities on the Chinese Loess Plateau (CLP) and their relationship to climate variation since 75 ka. δ13CSOM at Weinan ranges from −23.6 to −18.0‰ and δ15NSOM ranges from +3.4 to +5.5‰. Modeling based on the δ13CSOM record suggests that the proportion of C4 plants (%C4) has varied from lower during cold intervals (with minima of 24–32% at 73.1 ka, 20.9 ka (LGM), and 0.6 ka) to higher during warm intervals (with maxima of 62–64% at 53.0 ka and 8.2 ka). The δ13CSOM and δ15NSOM profiles are strongly correlated (r = +0.82; p(α) <0.001), suggesting that the N-isotope composition of SOM was also largely controlled by inputs of C4 versus C3 plants, consistent with known patterns of N-isotope variation among modern plants. The %C4 profile exhibits significant covariation with reconstructed mean annual temperatures (MAT) (r = +0.42; p(α) <0.001), mean annual precipitation (MAP) (r = +0.42; p(α) <0.001), and summer monsoon intensity (r = +0.50; p(α) <0.001), suggesting that paleovegetation was controlled by each of these climate variables to a degree. Comparing the Weinan record to SOM profiles across the CLP region, we identified three zones of varying monsoonal influence, with the East Asian Summer Monsoon (EASM) exerting the strongest influence on the southeastern margin of the CLP, and the East Asian Winter Monsoon (EAWM) greater influence on its northwestern margin. This paper provides new insights into deciphering paleovegetation patterns and their links to paleoclimate change on the basis of paired C- and N-isotope records.

Large regional-scale variation in C3/C4 distribution pattern of Inner Mongolia steppe is revealed by grazer wool carbon isotope composition

Abstract. This work explored the spatial variation of C3/C4 distribution in the Inner Mongolia, P. R. China, steppe by geostatistical analysis of carbon isotope data of vegetation and sheep wool. Standing community biomass (n=118) and sheep wool (n=146) were sampled in a ~0.2 Mio km2 area. Samples from ten consecutive years (1998–2007) were obtained. Community biomass samples represented the carbon isotopic composition of standing vegetation on about 1000 m2 ("community-scale"), whereas the spatio-temporal scale of wool reflected the isotope composition of the entire area grazed by the herd during a 1-yr period (~5–10 km2, "farm-scale"). Pair wise sampling of wool and vegetation revealed a 13C-enrichment of 2.7±0.7‰ (95% confidence interval) in wool relative to vegetation, but this shift exhibited no apparent relationships with environmental parameters or stocking rate. The proportion of C4 plants in above-ground biomass (PC4, %) was estimated with a two-member mixing model of 13C discrimination by C3 and C4 vegetation (13Δ3 and 13Δ4, respectively), in accounting for the effects of changing 13C in atmospheric CO2 on sample isotope composition, and of altitude and aridity on 13Δ3. PC4 averaged 19%, but the variation was enormous: full-scale (0% to 100%) at community-scale, and 0% to 85% at farm-scale. The farm-scale variation of PC4 exhibited a clear regional pattern over a range of ~250 km. Importantly PC4 was significantly higher above the 22°C isotherm of the warmest month, which was obtained from annual high-resolution maps and averaged over the different sampling years. This is consistent with predictions from C3/C4 crossover temperature of quantum yield or light use efficiency in C3 and C4 plants. Still, temperature gradients accounted for only 10% of the farm-scale variation of PC4, indicating that additional factors control PC4 on this scale.

Effects of temperature and fertilization on nitrogen cycling and community composition of an urban lawn

AbstractWe examined the influence of temperature and management practices on the nitrogen (N) cycling of turfgrass, the largest irrigated crop in the United States. We measured nitrous oxide (N2O) fluxes, and plant and soil N content and isotopic composition with a manipulative experiment of temperature and fertilizer application. Infrared lamps were used to increase surface temperature by 3.5±1.3 °C on average and control and heated plots were split into high and low fertilizer treatments. The N2O fluxes increased following fertilizer application and were also directly related to soil moisture. There was a positive effect of warming on N2O fluxes. Soils in the heated plots were enriched in nitrogen isotope ratio (δ15N) relative to control plots, consistent with greater gaseous losses of N. For all treatments, C4 plant C/N ratio was negatively correlated with plant δ15N, suggesting that low leaf N was associated with the use of isotopically depleted N sources such as mineralized organic matter. A significant and unexpected result was a large, rapid increase in the proportion of C4 plants in the heated plots relative to control plots, as measured by the carbon isotope ratio (δ13C) of total harvested aboveground biomass. The C4 plant biomass was dominated by crabgrass, a common weed in C3 fescue lawns. Our results suggest that an increase in temperature caused by climate change as well as the urban heat island effect may result in increases in N2O emissions from fertilized urban lawns. In addition, warming may exacerbate weed invasions, which may require more intensive management, e.g. herbicide application, to manage species composition.

Reconstructing past subsistence patterns on Zhongba Site using stable carbon and oxygen isotopes of fossil tooth enamel

The oxygen and carbon isotopic ratios in human and animal tooth enamel were measured to reconstruct the subsistence patterns on Zhongba Site between 2400 and 4200 aBP. The results indicate that human consumed chiefly C4 resources, such as millet, and the C3 plants, such as rice and wheat, constituted only a small fraction of the human diets, normally no more than 15%. There are significant differences in food sources between human and pig, and the percentages of C3 plants in pig diets were higher than those in human diets. But the δ 13C values of pig partially overlap those of human, demonstrating some similarities in their food sources. The differences in water sources between human and pig are significant. There are significant differences in food and water sources between cattle and deer. This indicates that the two kinds of mammals subsisted in different niches. But their δ 13C values also partially overlap each other, suggesting some similarities in their food sources. There are both significant differences in mean δ 13C and δ 18O values between the omnivores, human and pig and the herbivores, cattle and deer, implying significant differences in food and water sources between the two categories. During the period the dietary habits of human had not changed, but the pig breeding strategies had changed, from breeding in house to breeding in confinement, and the proportion of C3 plants in pig food increased with time. The scope of human migration had been considerably large by the Warring States because of the development of productive forces.

Carbon isotope evidence for an abrupt reduction in grasses coincident with European settlement of Lake Eyre, South Australia

Stable carbon isotopes in emu eggshell (EES) reflect emu diets and consequently the vegetation available for food sources. At Lake Eyre, South Australia, isotopic data suggest that there has been a rapid and dramatic change in vegetation at some point over the last 200 years when compared with the rest of the Holocene. The proportion of C4 plants in emu diets has been reduced by approximately 20% in response to an overall shift in C4/C3 biomass at Lake Eyre. Isotopic measurement of the dominant plants at Lake Eyre indicate that the C4 plants are almost entirely comprised of grasses and some chenopods and the C3 plants are comprised of the dominant chenopods, shrubs, trees and forbs. We surmise that the -20% reduction in C4 plant biomass reflects landscape degradation and loss of C4 grasses resulting from a combination of effects, including overgrazing by both introduced (e.g., sheep, cattle and rabbits) and native (e.g., kangaroos) animals, increasing drought and a change in fire regime beginning in the late 1890s. The magnitude of vegetation change that occurred in the last 200 years is as great as that which occurred during the last glacial maximum (-21 000 years ago), and provides the first evidence for major environmental change at Lake Eyre soon after Europeans settled the arid zone.