This study aimed to investigate the effects of a single bolus and continuous rate infusion (CRI) of 1% propofol on cholesterol and triglyceride levels of healthy bitches undergoing elective ovariohysterectomy. 10 healthy bitches undergoing elective ovariohysterectomy had blood samples obtained at baseline (TB), 15 minutes following premedication with acepromazine and morphine (TPM), after an intravenous bolus of propofol (induction to anesthesia, TIND) and following 90 minutes of CRI of propofol started at 0.4 mg kg−1 min−1 and adjusted according to individual requirements (TCRI). Data were initially tested for normality using the Shapiro-Wilk test, and comparisons were performed using Friedman followed by Dunn post-hoc test. Serum cholesterol levels significantly decreased at TIND and TCRI (median [min-max] 201 mg dL−1 [111-234 mg dL−1], and 215 mg dL−1 [111-239 mg dL−1]), respectively, compared with TB (232 [128-245 mg dL−1]) and TPM (206 [115-255 mg dL−1]). No differences were found between TIND and TCRI. Triglyceride levels increased significantly at TIND (120 [67-231 mg dL−1]) and TCRI (229 [73-549 mg dL−1]) compared with TPM (36 [51-29 mg dL−1]), and TCRI compared with TB. In conclusion, 1% propofol lipid emulsion significantly increases serum triglycerides and causes lipemia in healthy dogs at a single bolus or CRI.