Abstract In this study, whey protein isolate (W), κ-carrageenan (C), and a mixture of both (W–C, 1:1, w/w) containing pomegranate seed oil (PSO) was developed and characterized by scanning electron microscopy (SEM), water vapor permeability (WVP), solubility, mechanical, optical properties, release tests and antimicrobial activity. Film samples containing PSO showed higher elongation capacity, and lower WVP, solubility and transparency values (p