Properties Of Bone Tissue Research Articles

Composition and micromechanical properties of the femoral neck compact bone in relation to patient age, sex and hip fracture occurrence.

Current clinical methods of bone health assessment depend to a great extent on bone mineral density (BMD) measurements. However, these methods only act as a proxy for bone strength and are often only carried out after the fracture occurs. Besides BMD, composition and tissue-level mechanical properties are expected to affect the whole bone's strength and toughness. While the elastic properties of the bone extracellular matrix (ECM) have been extensively investigated over the past two decades, there is still limited knowledge of the yield properties and their relationship to composition and architecture. In the present study, morphological, compositional and micropillar compression bone data was collected from patients who underwent hip arthroplasty. Femoral neck samples from 42 patients were collected together with anonymous clinical information about age, sex and primary diagnosis (coxarthrosis or hip fracture). The femoral neck cortex from the inferomedial region was analyzed in a site-matched manner using a combination of micromechanical testing (nanoindentation, micropillar compression) together with micro-CT and quantitative polarized Raman spectroscopy for both morphological and compositional characterization. Mechanical properties, as well as the sample-level mineral density, were constant over age. Only compositional properties demonstrate weak dependence on patient age: decreasing mineral to matrix ratio (p = 0.02, R2 = 0.13, 2.6 % per decade) and increasing amide I sub-peak ratio I~1660/I~1683 (p = 0.04, R2 = 0.11, 1.5 % per decade). The patient's sex and diagnosis did not seem to influence investigated bone properties. A clear zonal dependence between interstitial and osteonal cortical zones was observed for compositional and elastic bone properties (p < 0.0001). Site-matched microscale analysis confirmed that all investigated mechanical properties except yield strain demonstrate a positive correlation with the mineral fraction of bone. The output database is the first to integrate the experimentally assessed microscale yield properties, local tissue composition and morphology with the available patient clinical information. The final dataset was used for bone fracture risk prediction in-silico through the principal component analysis and the Naïve Bayes classification algorithm. The analysis showed that the mineral to matrix ratio, indentation hardness and micropillar yield stress are the most relevant parameters for bone fracture risk prediction at 70 % model accuracy (0.71 AUC). Due to the low sample number, further studies to build a universal fracture prediction algorithm are anticipated with the higher number of patients (N > 200). The proposed classification algorithm together with the output database of bone tissue properties can be used for the future comparison of existing methods to evaluate bone quality as well as to form a better understanding of the mechanisms through which bone tissue is affected by aging or disease.

Osteoporosis detection with microwave signals: An investigation into natural resonance frequencies

This study analyses the dispersion behavior of microwave signals passing through biological tissues by considering various electromagnetic relaxation phenomena. The investigation concentrates on the impact of this dispersion behaviour on human bone tissues causing the health conditions of osteoporosis. Based on the attenuation profile through biological tissues, an optimum frequency range of operation is identified to capture the comprehensive target tissue profiles without minimal loss of signal strength in surrounding tissues. The dispersion through a human wrist model is analysed by extracting the late-time response. A pioneering aspect of this study lies in its utilization of the natural resonant frequency plane as a tool to gauge the dielectric properties of bone tissue, offering insights into its mineral content. To improve the ease of use and diagnostic accuracy, an automated multi-class classification using standard machine learning algorithms is developed during the final response analysis. The proposed approach effectively and accurately classifies the progression of osteoporosis.

In Vivo Assessment of Skin Surface Pattern: Exploring Its Potential as an Indicator of Bone Biomechanical Properties.

The mechanical properties of bone tissue are the result of a complex process involving collagen-crystal interactions. The mineral density of the bone tissue is correlated with bone strength, whereas the characteristics of collagen are often associated with the ductility and toughness of the bone. From a clinical perspective, bone mineral density alone does not satisfactorily explain skeletal fragility. However, reliable in vivo markers of collagen quality that can be easily used in clinical practice are not available. Hence, the objective of the present study is to examine the relationship between skin surface morphology and changes in the mechanical properties of the bone. An experimental study was conducted on healthy children (n = 11), children with osteogenesis imperfecta (n = 13), and women over 60 years of age (n = 22). For each patient, the skin characteristic length (SCL) of the forearm skin surface was measured. The SCL quantifies the geometric patterns formed by wrinkles on the skin's surface, both in terms of size and elongation. The greater the SCL, the more deficient was the organic collagen matrix. In addition, the bone volume fraction and mechanical properties of the explanted femoral head were determined for the elderly female group. The mean SCL values of the healthy children group were significantly lower than those of the elderly women and osteogenesis imperfecta groups. For the aged women group, no significant differences were indicated in the elastic mechanical parameters, whereas bone toughness and ductility decreased significantly as the SCL increased. In conclusion, in bone collagen pathology or bone aging, the SCL is significantly impaired. This in vivo skin surface parameter can be a non-invasive tool to improve the estimation of bone matrix quality and to identify subjects at high risk of bone fracture.

Enhanced biocompatibility and osseointegration properties of magnetron sputtered BTO-SZTO bio-piezoelectrically coated films as zirconium alloy implants

Bio-piezoelectric materials were capable of improving the osseointegration properties of implants by converting mechanical forces into electrical signals that provided microelectrical stimulation for the formation and remodeling of bone tissue. Although metallic bioimplant materials can provide adequate mechanical strength, their surfaces were biologically inert as well as lacking the piezoelectric properties to stimulate osteogenesis. In this study, perovskite piezoelectric materials (BaTiO3–SrZrTiO3) were uniformly deposited on the surface of a low-modulus zirconium alloy by magnetron sputtering, which endowed the surface of metallic biomaterials with favorable piezoelectric properties. Orthogonal tests were used to optimize the magnetron sputtering process parameters for the preparation of piezoelectric coatings. The morphology and electrical properties of the coated films were characterized by XRD, XPS, SEM, AFM and SKPFM. The piezoelectric constant of the piezoelectric coating prepared in this study was 5.02 pC/N, which approximated the piezoelectric properties of natural bone tissue. Biological experiments showed that the BaTiO3–SrZrTiO3 piezoelectric coated film had a good promotion effect on the early adhesion, proliferation and differentiation of osteoblasts. Compared with the traditional metal implant materials, this innovative method successfully simulated the piezoelectric properties of bone tissue on the material surface, resulting in better osseointegration properties of metal biomaterials. This method will provide a proven solution for surface modification of metallic implant materials.

Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone.

An innovative approach to treating bone defects is using synthetic bone substitutes made of biomaterials. The proposed method to obtain polylactide scaffolds using the phase inversion technique with a freeze extraction variant enables the production of substitutes with morphology similar to cancellous bone (pore size 100-400 µm, open porosity 94%). The high absorbability of the implants will enable their use as platelet-rich plasma (PRP) carriers in future medical devices. Surface modification by dipping enabled the deposition of the hydrophilic chitosan (CS) layer, maintaining good bone tissue properties and high absorbability (850% dry weight). Introducing CS increases surface roughness and causes local changes in surface free energy, promoting bone cell adhesion. Through this research, we have developed a new and original method of low-temperature modification of PLA substitutes with chitosan. This method uses non-toxic reagents that do not cause changes in the structure of the PLA matrix. The obtained bone substitutes are characterised by exceptionally high hydrophilicity and morphology similar to spongy bone. In vitro studies were performed to analyse the effect of morphology and chitosan on cellular viability. Substitutes with properties similar to those of cancellous bone and which promote bone cell growth were obtained.

Mechanotransduction and Biomechanical Properties of Bone Tissue as a Basis for the Use of Intraosseous Osteopathic Techniques

The development and maintenance of bone mass is critical to movement, health, and quality of life. Bone mass is regulated by various factors, activation of biologically active substances, enzymes, including changes in mechanical load. The osteocyte network provides a wide-ranging load monitoring "network" that penetrates every cubic millimeter of bone tissue. Deformities or trauma create a limitation that can cause asymmetric bone growth, such as plagiocephaly. A deep understanding of the cellular mechanisms responsible for bone remodeling as well as bone mechanotransduction is essential for the development of effective exercise, osteopathic techniques, and pharmaceutical strategies to increase and/or prevent bone loss. This review summarizes current data on the main molecular mechanisms involved in bone mechanotransduction, which provides insight into bone tissue, methods of working with it, and points of application for correcting various dysfunctions to promote bone growth in length. This process is carried out by increasing the proliferation of chondrocytes and the activity of growth plates. This contributes to the correction of such consequences of intraosseous dysfunctions as the development of anatomical differences in the size of the lower extremities in children.

STUDY OF THE EFFECTIVE PROPERTIES OF COMPACT BONE TISSUES

The paper investigates the effective properties of compact bone tissue under shear loading along the central axis of the Haversian canal. Compact bone tissue is considered as a structure consisting of an osteon (haversian canal, lamellae, cementing line) and interstitial tissue. To assess the effective longitudinal properties of bone tissue, a three-stage homogenization procedure based on the Reiss method and the three-phase method is used. The numerical values of the effective modulus of longitudinal shear obtained in the proposed work are in good agreement with the numerical values of the longitudinal shear obtained by other authors as a result of experimental studies.

POTENSI PENGGUNAAN β-TRICALCIUM PHOSPHATE SEBAGAI BAHAN SUBSTIUSI TULANG

Calcium phosphate has been widely used in bone regeneration applications. Originalkalsium fosfat merupakan biomaterial yang sering diaplikasikan ke dalam defek tulang dengan tidak memberikan efek toksik pada jaringan sehat.The most commonly used calcium phosphate are hydroxyapatite and β-tricalcium phosphate (β-TCP). β-TCP is one of the bioceramic materials that has chemical structure of β-Ca3(PO4)2. The use of β-TCP is widely used in medicine and dentistry as bone graft due to bone loss. β-TCP is used to fill and repair bone deficiencies to regenerative new bone formation. In dentistry, β-TCP can be used for periodontal disease therapy, pre-implant surgery to fill a post-extraction socket before implant application, post-surgical removal of benign tumors, post-endodontic bone healing such as apicoectomy and drug delivery. Various studies have shown β-TCP has better osteoconductive, biocompatible, biodegradable and bonding strength to bone tissue properties than hydroxyapatite. This article reviews the use of β-TCP as a substitute for hydroxyapatite in medicine and dentistry for bone substitution materials.

Fabrication and Evaluation of Porous dECM/PCL Scaffolds for Bone Tissue Engineering.

Porous scaffolds play a crucial role in bone tissue regeneration and have been extensively investigated in this field. By incorporating a decellularized extracellular matrix (dECM) onto tissue-engineered scaffolds, bone regeneration can be enhanced by replicating the molecular complexity of native bone tissue. However, the exploration of porous scaffolds with anisotropic channels and the effects of dECM on these scaffolds for bone cells and mineral deposition remains limited. To address this gap, we developed a porous polycaprolactone (PCL) scaffold with anisotropic channels and functionalized it with dECM to capture the critical physicochemical properties of native bone tissue, promoting osteoblast cells' proliferation, differentiation, biomineralization, and osteogenesis. Our results demonstrated the successful fabrication of porous dECM/PCL scaffolds with multiple channel sizes for bone regeneration. The incorporation of 100 μm grid-based channels facilitated improved nutrient and oxygen infiltration, while the porous structure created using 30 mg/mL of sodium chloride significantly enhanced the cells' attachment and proliferation. Notably, the mechanical properties of the scaffolds closely resembled those of human bone tissue. Furthermore, compared with pure PCL scaffolds, the presence of dECM on the scaffolds substantially enhanced the proliferation and differentiation of bone marrow stem cells. Moreover, dECM significantly increased mineral deposition on the scaffold. Overall, the dECM/PCL scaffold holds significant potential as an alternative bone graft substitute for repairing bone injuries.

Biomechanical comparison of compact versus standard flute drill bits, and interlocking versus buttress thread self-tapping cortical bone screws in cadaveric equine third metacarpal condyle.

To compare (1) performance of compact versus standard flute drill bits, (2) screw insertion properties and (3) pullout variables between interlocking thread (ITS) and buttress thread (BTS) self-tapping screws in third metacarpi. In vitro experimental study. Paired third metacarpi from 11 Thoroughbreds aged 2-4 years. Screws were inserted into the lateral condylar fossae following bone preparation using the respective drill bit for each screw type. Screw pullout was achieved using a mechanical testing system. Density and porosity of bone surrounding screw holes was measured with microcomputed tomography following each pullout test. Drilling, screw insertion and pullout variables were compared between drill bit and screw types using repeated measures ANOVA. Linear regression analyses were used to characterize relationships between bone tissue properties and drill bit and screw outcomes. Maximum torque power spectral density (PSD) was lower for compact flute drill bits. Insertion torque was 50% higher for ITS. BTS had 33% greater preyield stiffness and 7% greater mean yield force. Bone tissue properties affected measured variables similarly for both screw and drill bit types. Lower torque PSD may increase durability of the compact flute drill bit. ITS had greater insertional torque, which may reflect greater bone engagement. BTS had greater resistance to axial pullout forces. Metacarpal bone provides a simple model for comparison of drill bit and screw designs. Use of ITS to repair equine fractures subject to predominantly tensile forces is not justified based on the results of this study.

Mechanical properties and failure mechanisms of polyamide 12 gradient scaffolds developed with selective laser sintering

Developing a functional gradient scaffold compatible with the fantastic biological and mechanical properties of natural bone tissue is imperative in bone tissue engineering. In this work, the stretch-dominated (cubical and circular) and bending-dominant (diamond and gyroid) pore styles were employed to design custom-graded scaffolds based on the curve interference method and then were fabricated by selective laser sintering (SLS) using polyamide 12 (PA12) powder. Subsequently, the mechanical behavior, failure mechanism, and energy absorption performance of porous structures were investigated via compression experiments and finite element (FE) simulation. The results indicated that the stretch-dominated radial gradient structures entire exhibited transverse shear failure and the bending-dominant radial gradient structures whole exhibited progressive destruction, while all of the axial gradient scaffolds suffered a predictable layer-by-layer fracture. Among them, the bending-dominated radial gradient structure of gyroid had been proven to sustain stronger deformability and energy absorption capacity. Meanwhile, the FE method powerfully predicted the mechanical behavior of the scaffold, and this research thereby possessed significant implications for the development of bone tissue engineering.

Pulsed electrodeposition of MXenes/HAp multiple biological functional coatings on 3D printed porous Ti-6Al-4V bone tissue engineering scaffold

Titanium and its alloys have been widely used as bone tissue engineering scaffold due to their excellent biocompatibility and mechanical strength. In order to promote bone ingrowth, integration and match the mechanical properties of natural bone tissue, this study proposed diamond-like microstructure porous Ti-6Al-4V scaffolds for personalized bone defect repair. In order to prepare the multiple biological functional coatings on the curve surface inside of the 3D printed porous scaffolds, pulsed electrodeposition method was proposed to obtain multiple layers of Ti3C2Tx/HAp composited coatings. Delaminated Ti3C2Tx has an ultra-thin microstructure according to transmission electron microscopy (TEM) observation and is well dispersed in electrolyte. X-ray diffraction (XRD) characterization shows that Ti3C2Tx has been synthesized and regulates more CaHPO4‧2H2O (DCPD) to Hydroxyapatite (HAP) conversion, which is confirmed by Fourier transform infrared spectroscopy (FTIR) at 0.2 mg/mL concentration. Scanning electron microscopy (SEM) shows that the morphologies of composite coatings are closely related to Ti3C2Tx content due to the availability of effective voltages for electrodeposition. By means of finite element method, deposition thickness and distribution of coatings were simulated to explore the precipitation kinetics and selection of electrodeposition parameters and a current density of 15 mA/cm2 was ultimately used. In vitro electrochemical corrosion of Ti3C2Tx/HAp coatings were tested in human simulated body fluid (SBF) to verify its corrosion resistance in physiological environment and Ti3C2Tx/HAp composited coatings possess the best corrosion resistance according to linear scanning voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) tests. In vitro MC3T3-E1 cell culturing studies were investigated to evaluate cell behaviors and biocompatibility of these coatings. The results indicate that cells grow well on the surface of composite coatings without showing cytotoxicity and cell morphologies show a good spreading state and a trend of inward growth.

Resveratrol prevents ovariectomy-induced bone quality deterioration byimprovingthemicroarchitectural and biophysicochemical properties of bone.

Osteoporosis is a major health problem that is very common worldwide and is characterized by both low bone density and deterioration in bone quality. New treatment options without side effects have become an active area of research in recent years. This study was designed to investigate the preventive effects of resveratrol on bone quality deterioration caused by ovariectomy. Sixty rats were randomly divided into five groups (12 animals per group): Control, Sham-operated (SHAM), ovariectomized (OVX), OVX + Resveratrol-40mg/kg/day (OVX + Res40), OVX + Resveratrol-80mg/kg/day (OVX + Res80). Resveratrol was administered by oral gavage (40 and 80mg/kg/day) for ten weeks. Micro-CT measurements, biomechanical testing, Raman spectroscopyanalysis, and RT-PCR analysis were performed. ALP, OCN, TAS, and TOS levels were also measured from blood serum. Bone strength, bone volume/total volume, trabecular volume, and trabecular thickness were higher in the OVX + RES-80 group than inthe OVX group. Resveratrol increased osteogenic differentiation, as the expression of osteogenic markers ALP, Col1A1, Runx2, OPG, OCN increased in both OVX + RES-80 and OVX + RES-40 groups compared to theOVX group. 80mg/kg/day resveratrol administration decreasedthe levels of ALP, OCN and TOS in ovariectomized rats. Raman spectroscopy findings showed a preventive effect of resveratrol administration against ovariectomy-induced deterioration in biophysiochemical properties of bone tissue. This study revealed that administration of different doses of 80 mg/kg/day and 40 mg/kg/day of resveratrol had protective effects on bone quality deterioration caused by ovariectomy.

Biopolymer-based composites for tissue engineering applications: A basis for future opportunities

Biomimetic scaffolds supporting tissue regeneration are complex materials with multifunctional characteristics. The unique biocompatibility and biodegradability of biopolymers make them excellent candidates for tissue engineering and regenerative medicine. Biopolymers, which have a wide range of properties, can be obtained from different natural sources. Depending on the target tissue, biopolymers can be engineered to meet a series of specific functions. We review different types of biopolymers and their composites, besides their interactions with specific cells and tissues. Specific cellular mechanisms in tissue regeneration are also considered to elucidate the effects of biopolymers on controlling cellular mechanisms given their advantages and challenging aspects. Furthermore, the modifications required to mimic the properties of neural, cardiac, bone, and skin tissues are discussed. Utilization of biopolymer-based composites in tissue engineering requires additional improvements, where several challenges should be overcome. This work is mainly focused on biopolymers used in tissue engineering, providing support for engineering of future biocomposites for the same purpose. Some examples of biocomposites are also provided, a general guide for selection of biopolymers and the secondary component (biopolymers as complements, additives, or nano-scale biomaterials) to develop biocomposites.

The bioelectrical properties of bone tissue.

Understanding the bioelectrical properties of bone tissue is key to developing new treatment strategies for bone diseases and injuries, as well as improving the design and fabrication of scaffold implants for bone tissue engineering. The bioelectrical properties of bone tissue can be attributed to the interaction of its various cell lineages (osteocyte, osteoblast and osteoclast) with the surrounding extracellular matrix, in the presence of various biomechanical stimuli arising from routine physical activities; and is best described as a combination and overlap of dielectric, piezoelectric, pyroelectric and ferroelectric properties, together with streaming potential and electro-osmosis. There is close interdependence and interaction of the various electroactive and electrosensitive components of bone tissue, including cell membrane potential, voltage-gated ion channels, intracellular signaling pathways, and cell surface receptors, together with various matrix components such as collagen, hydroxyapatite, proteoglycans and glycosaminoglycans. It is the remarkably complex web of interactive cross-talk between the organic and non-organic components of bone that define its electrophysiological properties, which in turn exerts a profound influence on its metabolism, homeostasis and regeneration in health and disease. This has spurred increasing interest in application of electroactive scaffolds in bone tissue engineering, to recapitulate the natural electrophysiological microenvironment of healthy bone tissue to facilitate bone defect repair.

Biomechanical responses of the human lumbar spine to vertical whole-body vibration in normal and osteoporotic conditions

BackgroundThe prevalence of osteoporosis is continuing to escalate with an aging population. However, it remains unclear how biomechanical behavior of the lumbar spine is affected by osteoporosis under whole-body vibration, which is considered a significant risk factor for degenerative spinal disease and is typically present when driving a car. Accordingly, the objective of this study was to compare the spine biomechanical responses to vertical whole-body vibration between normal and osteoporotic conditions. MethodsA three-dimensional finite-element model of the normal human lumbar spine-pelvis segment was developed using computed tomographic scans and was validated against experimental data. Osteoporotic condition was simulated by modifying material properties of bone tissues in the normal model. Transient dynamic analyses were conducted on the normal and osteoporotic models to compute deformation and stress in all lumbar motion segments. FindingsWhen osteoporosis occurred, vibration amplitudes of the vertebral axial displacement, disc bulge, and disc stress were increased by 32.1–45.4%, 25.7–47.1% and 23.0–42.7%, respectively. In addition, it was found that for both the normal and osteoporotic models, the response values (disc bugle and disc stress) were higher in L4–L5 and L5–S1 intervertebral discs than in other discs. InterpretationOsteoporosis deteriorates the effect of whole-body vibration on lumbar spine, and the lower lumbar segments might have a higher likelihood of disc degeneration under whole-body vibration.

Acetabular Implant Finite Element Simulation with Customised Estimate of Bone Properties

The goal of the study is to analyse the strength and stability of a system comprising the pelvis and a customised implant under functional loads using the finite element method. We considered a technique for assessing the elastic properties of bone tissue via computer tomography, constructing finite element models of pelvic bones and a customised endoprosthesis based on the initial geometric models obtained from the National Medical Research Centre for Oncology n.a. N.N. Blokhin (Moscow, Russia). A series of calculations were carried out for the stress-strain state of the biomechanical system during walking, as well as at maximum loads when ascending and descending stairs. The analysis provided conclusions about the strength and stability of the studied device.

Improving the efficiency of methods for assessing bone tissue properties

Improving the efficiency of methods for assessing bone tissue properties

Experimentally characterizing the spatially varying anisotropic mechanical property of cancellous bone via a Bayesian calibration method

Traditional experimental tests for characterizing bone's mechanical properties usually hypothesize a uniaxial stress condition without quantitatively evaluating the influence of spatially varying principal material orientations, which cannot accurately predict the mechanical properties distribution of bones in vivo environment. In this study, a Bayesian calibrating procedure was developed using quantified multiaxial stress to investigate cancellous bone's local anisotropic elastic performance around joints as the spatial variation of main bearing orientations. First, the bone cube specimens from the distal femur of sheep are prepared using traditional anatomical axes. The multiaxial stress state of each bone specimen is calibrated using the actual principal material orientations derived from fabric tensor at different anatomical locations. Based on the calibrated multiaxial stress state, the process of identifying mechanical properties is described as an inverse problem. Then, a Bayesian calibration procedure based on a surrogate constitutive model was developed via multiaxial stress correction to identify the anisotropic material parameters. Finally, a comparison between the experiment and simulation results is discussed by applying the optimal model parameters obtained from the Bayesian probability distribution. Compared to traditional uniaxial methods, our results prove that the calibration based on the spatial variation of the main bearing orientations can significantly improve the accuracy of characterizing regional anisotropic mechanical responses. Moreover, we determine that the actual mechanical property distribution is influenced by complicated mechanical stimulation. This study provides a novel method to evaluate the spatially varying mechanical properties of bone tissues enduring complex mechanical loading accurately and effectively. It is expected to provide more realistic mechanical design targets in vivo for a personalized artificial bone prosthesis in clinical treatment.

Effect of L-tryptophan on the bone biophysical properties and oxygen consumption in rats with diet-induced obesity

The purpose of the study was to evaluate the effect of L-tryptophan on the biophysical properties of bone tissue and oxygen consumption in rats with diet-induced obesity. The study was conducted on 40 male 3-months- aged Wistar rats. The photometric determination of phosphorus and calcium concentration in the femoral bones was conducted. The rate of oxygen consumption was measured according to chronoamperograms. The biophysical properties of femurs were measured by a methodic three-point bending test. It was shown that the content of calcium (by 15%) and phosphorus (by 20%) was significantly higher in the femurs of rats that received L-tryptophan (at a dose of 80 mg/kg per os) for 28 days compared to the control parameters. The rate of oxygen consumption, density and biophysical properties of the femurs did not change. As a result of the consumption for three months of a high-calorie diet (580 kcal/100 g) in experimental rats clear features of obesity evolved. Thus, they had a greater visceral fat mass (by 145%), a visceral fat mass to body weight ratio (by 122%), and an obesity index (by 145%). In rats with alimentary obesity, the calcium and phosphorus content in the femurs was significantly reduced by 28 and 24%, respectively, and the rate of oxygen consumption was 45% lower than in control animals. Femur bearing capacity, strength limit, and stiffness were significantly lower, namely on 23, 11, and 37%, respectively. Administration of L-tryptophan to rats, against the background of consumption of a high-calorie diet, inhibited the development of obesity. Visceral fat mass and its ratio to body weight in this group of rats were 38 and 23% lower, respectively, compared to the obese group. The concentration of calcium (by 32%) and phosphorus (by 25%) and oxygen consumption rates (by 31%) were significantly greater compared to rats fed only the high-calorie diet. Our research shows that the administration of L-tryptophan to obese animals can prevent the development of negative changes in bone tissue.