Properties For Tissue Engineering Research Articles

HA/TCP Scaffolds Coated by PLA and Gelatin: Preliminary <i>In Vitro</i> Evaluation

The importance of this work is development of two methods to try to improve the bone tissue regeneration. The surface of scaffold was modified in order to favor cell interaction, through adhesion and proliferation. PLA and gelatin were used. PLA has shown in literature good results in bone tissue engineering. The gelatin is used as coating in cell culture plates to improve cell attachment for a variety of cell types, including osteoblast. Hydroxyapatite (HA) and tricalcium phosphate (TCP) were used due to its known properties in tissue engineering. It was used the polymer foam replication technique to produce the scaffolds. For characterization were used: scanning electron microscopy (SEM), optical microscopy (OP), stereoscopy, transmission electron microscopy (TEM), X-ray microtomography (Micro-CT), X-ray diffraction (XRD) and X-ray fluorescence (XRF). The scaffolds showed morphology with adequate porosity for tissue engineering and thein vitrotest showed evidence of not being cytotoxic.

The Use of Nanocomposite Coatings with Various Physicochemical Properties in Tissue Engineering

We studied the effect of nanocomposite coatings with various physicochemical properties on the structural and functional properties (adhesion potential, phenotype, gene expression) of mesenchymal stem cells. Of all tested nanocoatings (Al2O3, ZrO2, Ta2O5), oxide coating Al2O3 enriched in vitro monolayer bone marrow cell culture with cells carrying mesenchymal stem cells phenotype markers and stimulated expression of ido gene, which can confer new therapeutic potencies to these cells and extend their application in clinical practice.

Synthesis and characterization of nanocrystalline hydroxyapatite gel and its application as scaffold aggregation

The sol-gel process is a technique used to synthesize materials from colloidal suspensions and, therefore, is suitable for preparing materials in the nanoscale. In this work hydroxyapatite was used due to its known properties in tissue engineering. Hydroxyapatite Ca10(PO4)6(OH)2 is a bioactive ceramic which is found in the mineral phase of bone tissue and is known for its great potential in tissue engineering applications. For this reason, this material can be applied as particle aggregates on ceramic slurry, coating or film on materials with a poorer biological response than hydroxyapatite. In this work, hydroxyapatite gel was obtained by the sol-gel process and applied as nanoparticle aggregation in the mixture of hydroxyapatite and tricalcium phosphate to form a ceramic slurry. This process is the polymer foam replication technique used to produce scaffolds, which are used in tissue engineering. For HA gel characterization it was used enviromental scanning electron microscopy (ESEM), transmission electron microscopy (TEM), electron energy loss spectroscopy (EELS), scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray fluorescence (XRF). The crystallite size was calculated from XRD data using the Scherrer equation. The nanoparticles size before firing was approximately 5nm. The crystallite size calculated after calcination was approximately 63 nm. The EELS results showed that calcium phosphate was obtained before firing. After HA gel calcination at 500 ºC the XRD results showed hydroxyapatite with a small content of beta-TCP. The scaffolds obtained by polymer foam replication technique showed a morphology with adequate porosity for tissue engineering.