With the increased growth in the cosmetic industries, it has become quite common to seek the services of professional beauticians thereby increasing the tendency of sharing makeup applicators. This study investigates the microbiological contamination of makeup applicators (Brushes and sponges) used by beauticians and to determine the level of hygiene practices. Microbial contamination was detected from Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus and fungi with the highest percentage occurrence observed in brushes for Pseudomonas aeruginosa and Bacillus at 100% while fungi was highest in sponges with 70%. The hygiene assessment was analyzed using a structured questionnaire to ascertain the level and type of hygiene measures taken in cleaning the applicators used on their clients; the result obtained showed that less than 50% do not discard their old makeup applicators as often as they should (2.4bc ± 0.92) and do not clean their applicators with alcohol based agent (1.8a ± 1.25). The repetitive use of these applicators on clients especially over time without proper hygiene measures is the major cause of contamination. These makeup sponges and brushes have shown to be vehicle for transmission of pathogens and hygiene practices should be taken more seriously especially when dealing with multiple clients.