Propagation Of Cuttings Research Articles

Genome-Wide Identification and Analysis of the Aux/IAA Gene Family in Rosa hybrida—“The Fairy”: Evidence for the Role of RhIAA25 in Adventitious Root Development

Propagation of cuttings is the primary method of rose multiplication. Aux/IAA, early response genes to auxin, play an important role in regulating the process of adventitious root formation in plants. However, systematic research on the identification of RhAux/IAA genes and their role in adventitious root formation in roses is lacking. In this study, 34 RhAux/IAA genes were identified by screening the rose genome, distributed on seven chromosomes, and classified into three clades based on the evolutionary tree. An analysis of the cis-acting elements in the promoters of RhAux/IAA genes revealed the presence of numerous elements related to plant hormones, the light signal response, the growth and development of plants, and abiotic stress. RNA-seq analysis identified a key RhIAA25 gene that may play an important role in the generation of adventitious roots in roses. Subcellular localization, yeast self-activation, and tissue-specific expression experiments indicated that RhIAA25 encoded a nuclear protein, had no yeast self-activated activity, and was highly expressed in the stem. The overexpression of RhIAA25 promoted the elongation of the primary root in Arabidopsis but inhibited adventitious root formation. This study systematically identified and analyzed the RhAux/IAA gene family and identified a key gene, RhIAA25, that regulates adventitious root generation in roses. This study offers a valuable genetic resource for investigating the regulatory mechanism of adventitious root formation in roses.

Significance of reeling waste water on the biometric traits of mulberry (Morus Sp.)

The present study was conducted in Department of sericulture, Forest college and research institute, Mettupalayam. In the study, we analysed the impact of reeling waste water on the plant propagation and growth of mulberry cuttings, reeling water samples were collected from reeling units in vadavalli, coimabtore district. Cuttings of V1 and G4 were selected and maintained in a pot culture, frequently irrigated with reeling water at different concentrations. Observations pertained to treatments were calculated at 30,60 and 90 days after planting, from that recordings, present investigation was concluded that the sprouting and other studied biometric traits except internodal distance in both V1 and G4 shows higher value at lowest concentration of reeling waste water i.e., T2 (25% reeling waste water + 75% tap water). Highest concentration of reeling waste water reduces the sprouting as well as growth of the mulberry cuttings due to the presence of more amount of elements which affects the metabolic activities of the plants. Except T5 (100% reeling waste water) all other concentrations were exhibited better propagation and growth of mulberry cuttings.

Effect of Aloe Vera, Wood Ash, and Indole-Butyric Acid on In-Vivo Macro Propagation of Robusta Coffee Stem Cuttings

Purpose: This study investigated the influence of Aloe Vera, Wood ash and a synthetic growth hormone Indole-butyric acid (IBA) on root and shoot in the proliferation of Coffee Wilt disease-resistant (CWD-R) Robusta coffee stem cuttings. Methodology: Stem cuttings of CWD-R Robusta coffee were treated with five levels of rooting natural substances and IBA independently and in combination. The treated cuttings were propagated in a uniform potting medium under humidity chamber conditions. Data was collected on the number of surviving cuttings, shoot and root number, and root and shoot lengths. At 90 days after planting, data on root proliferation, the number, and lengths of roots were collected and analyzed for variance (ANOVA) using GenStat 11th Edition. Findings: The survival, root, and shoot development were significantly (p<0.05) influenced by the rooting substances applied. A combination of Aloe Vera and IBA, 0.8% IBA, and Aloe Vera gel applied alone gave the highest percentages (90, 70 and 70%, respectively) of stem cuttings that survived to form roots and shoots. A mixture of aloe Vera and IBA, 0.8% IBA, and Aloe Vera gave the highest mean number of shoots and roots per cutting; that is (8.75, 8.5, and 8.5 cm) and (13.87, 12.85 and 12.39 cm) for shoots and roots respectively. Cuttings treated with wood ash and control gave the lowest mean number of shoots and roots that formed per cutting; that is (5.25 and 4.50 cm) and (11.75 and 11.38 cm) for shoots and roots respectively. Unique Contribution to Theory, Practice and Policy: Propagation of Coffea canephora, by stem cuttings can be adequately achieved by using Aloe Vera, IBA, or their mixture, but not with wood ash.

ROOTING OF JUVENILE CUTTINGS OF Pinus patula Schiede ex Schltdl. et Cham. HEDGES

Pinus patula Schiede ex Schltdl. et Cham. is endemic to Mexico and is used in both reforestation programs and in the timber industry due to its fast growth, wood quality, and easy handling. With the advance in genetic breeding programs, there is a need to massively propagate new plants from cuttings. One of the main challenges for this purpose is to know the effect of the age of the mother plant (hedge) on the rooting of cuttings. The younger the plant, the greater the rooting is expected to be. Three ontological ages of P. patula mother plants were compared (7, 10, and 13 months). The experimental design was in complete random blocks, with four replications and 25 cuttings per experimental unit. The response variables were evaluated 20 weeks after the experiment was established. No significant differences were observed in the rooting of the three ages evaluated (67.3 to 81 %), nor in the quality of the root (number and length of primary roots, percentage of plants with secondary roots). The results point out an advantage for the propagation of Pinus cuttings since the mother plants at those ages are capable of generating juvenile cuttings that are adequate to obtain percentages of rooting of over 75 %.

Design and Optimization of Air Inlet in Cuttings Incubator

The microclimate environment can be conveniently controlled with accuracy by plant incubators, in which the cuttings propagation method can efficiently enhance seedling production. To ensure air flow evenly throughout the incubator, the scientific design of the air inlet is crucial. This study utilized a computational fluid dynamics (CFD) model to simulate the airflow patterns in a culture layer under different air inlet conditions. Furthermore, the optimal design parameters were determined by way of response surface methodology (RSM) and the Non-dominated Sorting Genetic Algorithm-II (NSGA-II). Adopting the optimal parameters, a prototype was manufactured, and a cuttings experiment was carried out with apple cuttings in the incubator. The results showed that the optimal air inlet radius is 90 mm, the optimal air inlet height is 188 mm, and the optimal uniform flow plate hole diameter is 13 mm. Meanwhile, the apple cuttings were able to root. Therefore, this incubator with optimal parameters can be used for cuttings. The study provides a methodological and theoretical basis for the future optimizing of air inlet parameters and promoting cuttings rooting.

Effect of different soil media on rooting of dragon fruit cuttings

The present study was carried out at the Fruit Nursery, Department of Fruit Science, Dr Panjabrao Deshmukh Krishi Vidypeeth, Akola.With the aim to investigate the effect of various compositions of soil media and to find the proper composition for better rooting of dragon fruit cuttings. The design of experiment was CRD with five replications consisting of seven treatment combinations viz., T1-Soil:Sand:FYM, T2-Soil:Red Soil:FYM, T3-Soil:Silt:FYM, T4-Soil:Sand:Vermicompost, T5-Soil:Red Soil:Vermicompost, T6-Soil:Silt:Vermicompost with ratio of 2:1:1 and T7 was control (100% Soil). All the data were recorded on different growth parameters of selected representative cuttings which were selected at random from each replication. Treatment T5 i.e. Soil:Red Soil:Vermicompost in ratio 2:1:1 displayed superior performance articulated in terms of root growth parameters viz., number of root per cutting (58.2), root length (21.54 cm), root diameter (2.36 mm), length of longest root (23.66 cm), average number of roots (53.62), average length of root (20.96 cm), root volume (1.82 cc), fresh weight of root (5.18 g), dry weight of root (0.86 g) and survival percentage (97.59%) among different treatments for successful propagation of dragon fruit cuttings.

Evaluating the impact of phytosynthesized micronutrient nanoparticles on the growth and propagation of mulberry cuttings: dose determination and toxicity concerns

Impact of micronutrient nanoparticles on mulberry.

The The Effect of Nutrients (N and P) and Hormone (IAA) Application on the Growth of Cocoa (Theobroma cacao L.) Plagiotrope Cuttings

To support the successful propagation of cocoa plagiotrope cuttings, nutrients (N and P) and hormone (IAA) are applied to increase plant growth. The purpose of this research was to determine the effect of the provision of nutrients (N and P) and hormone (IAA) on the growth of cocoa plagiotrope cuttings. The experiment was carried out factorial with the basic pattern of Completely Randomized Design (CRD) and was repetitively replications by three times. The first factor was the dose of urea (N) and SP-36 (P) which consisted of 3 levels, such as P0 (0 g/polybag urea and 0 g/polybag SP-36), P1 (3,5 g/polybag urea and 2,5 g/polybag SP-36), and P2 (7 g/polybag urea and 5 g/polybag SP-36). The second factor was the concentration of IAA which consisted of 5 levels, such as I0 (0 ppm), I1 (50 ppm), I2 (100 ppm), I3 (150 ppm), and I4 (200 ppm). The results showed (1) the interaction between the application of the nutrients (N and P) and hormone (IAA) had no significant effect on all observed variables except the number of leaves, where the best treatment combination was the application of fertilizer doses of 0 g/polybag urea + 0 g/polybag SP-36 and IAA concentration of 100 ppm (P0I2) (2) application of the nutrients (N and P) with dose of P1 increased seedling growth on all observed variables except the number of leaves, shoot diameter, and shoot length (3) application of IAA with concentration of I1 increased seedlings growth, that is the number of primary roots/cuttings and shoot length.

Effects of Different Root Inducing Agents on Cutting Propagation of Tea (Camellia sinensis)

Aims: In commercial cultivations, vegetative propagation through rooting of cuttings is common, and IBA- based rooting hormone is often used. However, due to the need for non-chemical alternatives in organic cultivations, organic rooting substances have become increasingly important. Hence considering this, an experiment was conducted to investigate the effectiveness of different root-inducing agents on the propagation of tea cuttings.
 Study Design: The experimental units were arranged in a completely randomized design.
 Place and Duration of Study: The experiment was conducted in a special project nursery belonging to TSHDA (Tea Small Holding Development Authority) in Beralapanathara GN division of Pasgoda divisional secretariat in Matara, Sri Lanka.
 Methodology: The nodal cuttings were pre-treated with six different root-inducing agents including water (control), Aloe vera gel, coconut water, honey charcoal mixture, potato juice and rooting hormone (0.3% IBA). Each treatment was replicated four times with 20 cuttings in each. Cuttings' survival percentage, rooting percentage, the number of roots, root length and fresh and dry weights of the roots were evaluated during three phases as 2nd, 4th and 6th week. The data were statistically analyzed using SAS statistical package and treatment means were compared using DMRT at 5% significance level.
 Results: The results revealed that there were significant differences between the treatments on cutting performances. The cuttings treated with rooting hormone showed the highest values in rooting percentage (55.8%), sprouting percentage (72.3%), during the 4th week and root length (2.1cm) and root dry weight (0.254g) during the 6th week. Moreover, coconut water and Aloe vera gel showed no significant higher values in most of the measured variables compared to the rooting hormone.
 Conclusion: Therefore, it can be concluded that coconut water and Aloe vera gel can be considered alternative rooting substances to the chemical-based rooting hormone for organic tea cultivations.

Zinc oxide nano-flowers improve the growth and propagation of mulberry cuttings grown under different irrigation regimes by mitigating drought-related complications and enhancing zinc uptake

Silkworm larvae mainly consume mulberry leaves; therefore, mulberry cultivation is important for the production of raw silk. Drought stress and micronutrient deficiency (Zn) are known to affect the propagation of mulberry cuttings. In this purview, the current investigation attempted to inspect the efficacy of different concentrations of zinc oxide nano-flower (ZnNFs) applied through both soil admixture and foliar spray on the propagation of mulberry cuttings grown under deficit irrigation regimes. The overall results demonstrated that the ZnNF-treated plant cuttings were well-adapted to drought stress and performed better in comparison to the control set. Out of the tested concentrations – ZnNF-10 (applied as 10 mg/kg soil and 10 ppm as foliar spray thrice) was found to be optimum, showing relatively better initial root establishment, the emergence of leaves, and survival and sprouting percentage. Further studies also confirmed an improvement in the accumulation of photosynthetic pigments, carbohydrates, and protein content even under extreme drought conditions. Most importantly, the ZnNF-10 treatment contributed to ROS detoxification and cell membrane protection by enhancing the pool of antioxidant enzymes. The study further demonstrated that ZnNF-10 application enhanced zinc content by 147.50%, 179.49%, and 171.99% in root, shoot, and leaves of the treated cuttings; thereby, improving the bioaccumulation factor of the plant parts. All of these interactive phenomena led to an increment in shoot height, biomass, leaf area, and leaf number of cuttings. These findings, therefore, indicated that ZnNFs can be developed as a promising nano-fertilizer for mulberry growth facilitating Zn uptake and mitigation of drought-induced complications.

Response of Growth and Yield of Mint (Mentha spicata L.) Cuttings to Auxin and Composition of Planting Media

Consumption of herbal medicines by Indonesian people is increasing because of the price and ease of obtaining them. One of the main ingredients of herbal medicine is mint leaves because they contain tannin and flavonoid secondary metabolites. However, the generative mint plant is very slow and needs vegetative cuttings propagation. This study aims to examine the effect of auxin-type growth regulators and the composition of the growing media on the growth and yield of mint plant cuttings. This study used a complete randomized block design with two factors. The first factor was the auxin growth regulator with four levels: without growth regulator, IBA 500 ppm, IAA 500 ppm, NAA 500 ppm. The second factor was the composition of the planting medium with three levels, namely soil, husk charcoal, and goat manure 3:3:1; 3:2:2, and 3:1:3. Three repetitions were carried out. Auxin affects the number and area of leaves. Applying 500 ppm NAA encouraged the highest leaf growth, with the number of leaves at 163.38. Growth regulator NAA 500 ppm increased the number of leaves and leaf area of mint plants, i.e., by 0.01% and 1.16% compared to the control. The growing media composition affected the crown fresh weight, root biomass, and crown root ratio. The media composition of 3:3:1 produced a crown fresh weight of 41.07 g, and the media composition 3:3:3 produced the highest biomass of 5.97 g. The media composition 3:1:3 showed the highest root crown ratio, 2.69%. Auxin and the composition of the growing media synergistically promote the growth and yield of mint cuttings.

Standardization of growing substrate for multiplication of guava (Psidium guajava) through cuttings

Experiments under the polyhouse were conducted for two consecutive years (2021 and 2022) at Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Jammu and Kashmir to evaluate the effect of different growing substrates on guava (Psidium guajava L.) multiplication through cuttings. Twelve substrate combinations were tried in polybags and polytrays under a polytunnel in a polyhouse provided with mist irrigation. The study revealed a positive effect of different growing substrates on growth parameters and successful propagation of guava cuttings. The perusal of the pooled data revealed that T9 was the most effective growing substrate which recorded maximum rooting percentage, survival percentage, plant height, fresh and dry weight, the diameter of thickest sprout, shoot length, length of longest root, leaf area, and the number of roots, shoots, leaves per longestshoot along with minimum mortality percentage and days to first sprouting. However, some growth parameters such as stem thickness, chlorophyll percentage, number of sprouts, sprouted cuttings, and leaves of planted guava cuttings yielded the best results in T10. Cuttings grown in topsoil only without any growing substrate (T12) provided the least values of all the growth parameters of guava cuttings.

The effects of different rooting temperatures and phytohormones on the propagation of boxwood cuttings

Due to its wide range of utilization possibilities, boxwood has undergone severe damage, like all over the world, caused by the excessive forest use in Türkiye from the past to the present. Therefore, it is important to propagate it to ensure the continuity of the species. In this study, the effects of different rooting table temperatures and phytohormones on rooting were investigated in the propagation of Buxus sempervirens L. subsp. sempervirens by softwood cutting. First callus and root formation dates, rooting percentage, callus percentage, root length and the number of roots were determined on the cuttings. As a result, the highest rooting percentages were obtained as 100% in IBA 3,000, IBA 5,000, IAA 3,000, NAA 3,000 and NAA 5,000 ppm treatments in Greenhouse-1 medium and IBA 3,000 and IAA 3,000 ppm treatments in Greenhouse-2 medium. In terms of root length and the number of roots, the highest values were determined as 2.01 cm and 7.83, respectively, in IBA 3,000 ppm treatment in Greenhouse-2 medium. With the high success obtained, it has been concluded that the propagation of the species can be made by using softwood cutting and the genetic infrastructure of the species can be preserved and transferred to future generations. Keywords: Buxus sempervirens, ornamental plant, cutting propagation, auxin

Impact of PGPR Formulations Combined with Exogenous IBA Levels to Enhance Root Capacity in Poinsettia Cuttings

The commercial production of poinsettias begins with the propagation of apical cuttings from stock plants. The aim of the study was to use bacterial mixtures prepared with PGPRs in the cultivation of stock mother poinsettia plants and determine an effective IBA dose to increase root development and root yield of cuttings prepared from these stock mother plants. Rooted cuttings of Euphorbia pulcherrima Willd.ex Klotzsch were used for planting stocks of mother plants. Eight different bacterial isolates were mixed in triple combinations to form 4 different bacterial formulations: (BI) formulation 1 (Paenibacillus polymyxa TV-12E + Pseudomonas putida TV-42A + Pantoea agglomerans RK-79); (BII) formulation 2 (Bacillus megaterium TV-91C + Pantoea agglomerans RK-92 + Bacillus subtilis TV-17C); (BIII) formulation 3 (Bacillus megaterium TV-91C + Pantoea agglomerans RK-92 + Kluyvera cryocrescens TV-113C); and (BIV) formulation 4 (Bacillus megaterium TV-91C + Pantoea agglomerans RK-79 + Bacillus megaterium TV-6D). In the first year, rooted cuttings of stock mother plants were dipped in liquid microbial solution (bacterial formulations) for 15 min and then planted in plastic pots. In the second year, the same bacterial formulations were freshly prepared and applied to the 1-year-old stock mother plants as irrigation water for the second time. Amounts of 0 (control), 1000, 1500, and 2000 mg L−1 IBA doses were used for rooting cuttings taken from the 2-year-old stock mother plants. In the experimental group treated with the BI bacterial formulation, the number of rooted cuttings (NR) increased by 14.26% and 19.00%, compared with the control, in response to IBA 1500 mg L−1 and IBA 2000 mg L−1 treatment, respectively. Compared to the mean root length of the cuttings in the control treatment, the mean root length of the cuttings in the BIV treatment increased by 18.83%. The mean root length values decreased with the increase in IBA doses. The highest value of the number of mean shoots (NAS) was in cuttings treated with BI + 1500 treatment, which was 1.93 times higher than the control. The highest nitrogen content (5.73%) was determined in the bract leaf samples of the BIV application, and an 81.33% nitrogen increase was detected in the related application when compared to the control. In terms of P, Ca, and Fe contents, higher values were obtained from the BIV application when compared to the control application. The highest values of K content were determined in BIII and BII applications. This study provides positive effects on the feedback of stock mother plants with PGPR to provide sprout production by cuttings technique. It has been revealed that lower IBA dose applications can be recommended for rooting cuttings taken from mother plants treated with the BIV bacterial formulation.

Aplikasi NAA pada Propagasi Kantong Semar Nepenthes adrianii Batoro, Wartono & Jebb

Nepenthes adrianii is an endemic species of Mount Slamet. The existence of this species has begun to be threatened. This situation encourages efforts to conserve N. adrianii, which is through propagation using stem cuttings of N. adrianii. In order for the propagation of stem cuttings to grow properly, it is necessary to add growth hormone (ZPT) from the auxin group such as NAA (Napthalene Acetic Acid). This research has been conducted to study the effect of NAA and its effective consentration for the growth of N. adrianiistem cuttings. This research was conducted using experimental methods. The experimental design used was a Randomized Completely Block Design (RCBD). The block in this study is total leaves per cuttings. Block 1 is the leafless cuttings, block 2 is cuttings with one leaf, block 3 is cuttings with two leaves, and block 4 is cuttings with three leaves. The NAA concentrations in this study are 0, 5, 10, 15, 20, and 25 ppm. There are a total of 24 experimental units. The variable observed was the growth of N. adrianii stem cuttings. Parameters observed in this study are days taken for sprouting, number of shoots, number of leaves, leaf length, and leaf width. The results were analyzed using Variety Analysis and then analyzed with Tukey's test at the test level of 0.05 at the time of significant treatment. The results showed that the application of NAA did not affect the growth of the stem cuttings of N. adrianii. The best concentration of NAA could not be determined because there was no effect of NAA on the growth of N. adrianii stem cuttings.

TERMITES AND Rhizoctonia sp.: MAJOR PROBLEMS IN ROBUSTA COFFEE CUTTINGS CULTIVATED ON DIFFERENT PLANTING MEDIA

Robusta coffee is one of the plantation commodities vegetative propagated through cuttings to keep them in the same characteristics as their parents. The main problems in cuttings propagation are pests and diseases are cause damage to the planted cuttings. This research aimed to study the causal agent and its damage to robusta coffee cuttings planted on different media. The research was conducted from September 2020 to March 2021 in the experimental field station (KP) Pakuwon and the Integrated Laboratory of Balittri. The arrangement of the experimental design was a split plot with two repetitions. The main plot was the type of media planted consisting of sand, sand + fertilizer (1: 1), sand + soil (1: 1), soil + fertilizer (1: 1), sand + soil + fertilizer (1: 1: 1). The subplot was a Robusta coffee clone consisting of BP308, BP393, and SA203. The results showed that the highest percentage of damage caused by termites and Rhizoctonia sp. was observed on the cuttings planted on sand + fertilizer mixture media while the lowest was on sand media. The termites found in the robusta coffee cuttings were Macrotermes gilvus.

THE EFFECT OF ROOTING MEDIA ON THE GROWTH OF MALAY APPLE (Syzygium malaccense L.) SEEDLINGS FROM STEM CUTTINGS

This study aims to determine the effect of the type of planting medium in the propagation of Malay apple cuttings. The research design used is random design non-factorial grouping that consist of 5 levels of treatment for cuttings rooting media types, i.e. soil (1), soil + compost (1:1), soil + compost + chaff charcoal (1:1:1), sand + compost + chaff charcoal (1:1:1), and compost (1) with 5 repetitions and each replication consists of 25 experimental units, and each experimental unit consists of 15 samples and 3 spare plants, so there are 450 plants. The research was conducted from February to July 2022. The parameters observed include the time of shoot emergence, sprout percentage, root length, seed height, root volume, and the percentage of life. Data analysis used ANOVA (analysis of variance) and if there was an effect, then continued with the tukey’s HSD (Honestly Significant Difference) test with a level of 5%. The results showed that rooting media mixed with soil + compost + chaff charcoal or mixed media of sand + compost + chaff charcoal could increase the percentage of survival, seedling height, root length, and root volume of malay apple seedlings from stem cuttings.

The Effect Of Growtone As Growth Regulatory Substance On The Growth of Stock Steps Of Red Dragons Fruit (Hylocereus costaricensis Britton & Rose)

Dragon fruit plants can be propagated generatively by seeds and vegetatively by propagation of branch or stem cuttings. This study aims to examine the effect of Growtone's growth regulator on the growth of red dragon fruit stem cuttings. The treatment used Growtone with three concentration levels, namely 0 (without PGR as a control), 10 g ZPT/liter water, 15 g ZPT/liter water, and 20 g ZPT/liter water. The study was designed using a Randomized Block Design with six replications. Each experimental unit consisted of 10 plants. The plant growth responses measured were shoot growth time, number of shoots, shoot length, number of roots, root length, and root dry weight. The results of the study found that PGR Growtone had an effect on shoot length, root length, number of roots and root dry weight, but had no effect on shoot growth time and number of shoots. It is better to give Growtone ZPT with a concentration of 20 g/l water

Potential Functional Food Products and Molecular Mechanisms of Portulaca Oleracea L. on Anticancer Activity: A Review

Portulaca oleracea Linn. (P. oleracea L.) has recently gained attention as a functional food due to the chemical composition of this plant regarding bioactive compounds. The special attention to the use of P. oleracea as an ingredient in functional food products is also due to the promotion of sustainable food. It is an unconventional food plant, and its consumption may contribute to preserving biodiversity due to its cultivation in a polyculture system. Food sovereignty may be achieved, among other strategies, with the consumption of unconventional food plants that are more resistant in nature and easily cultivated in small places. P. oleracea grows spontaneously and may be found in streets and sidewalks, or it may be cultivated with seeds and cuttings propagation. The culinary versatility of P. oleracea opens up opportunities to explore the development of sustainable, functional food products. This mini-review shows that functional food products developed from P. oleracea are already available at the research level, but it is expected that more scientific literature focusing on the development of P. oleracea functional products with proven anticancer activities may be released in the near future. Polysaccharides, some phenolic compounds, alkaloids, and cerebrosides are associated with the inhibition and prevention of carcinogenesis through in vitro and in vivo investigations. The anticancer activities of P. oleracea, its bioactive compounds, and the involved molecular mechanisms have been reported in the literature. The importance of further elucidating the cancer inhibition mechanisms is in the interest of forthcoming applications in the development of food products with anticancer properties for implementation in the human diet.

Evaluation of One-Time Applications of Foliar Applied Auxin Co-Applied with Surfactant for Use in Commercial Cutting Propagation

Use of foliar auxin applications are increasing in the nursery and greenhouse industry. However, previous research has shown that insufficient auxin is absorbed or translocated to the site of action when foliar auxin applications are used. It is theorized that adding surfactants to foliar applications of auxin may help with the absorption and translocation of auxin to the site of action. Research was conducted to determine whether adding surfactants to one-time foliar applications of indole-3-butyric acid (IBA) would be as effective as the current industry standard, the basal quick-dip. Terminal, semi-hardwood cuttings of Red Cascade™ miniature climbing rose (Rosa ‘MOORcap’), common camellia (Camellia japonica) and ‘Southern Charm’ magnolia (Magnolia grandiflora ‘Southern Charm’) were sprayed to the drip point using Hortus IBA Water Soluble Salts™ at concentrations of 0 ppm, 50 ppm, 75 ppm, or 100 ppm for rose cuttings or 0 ppm, 500 ppm, 1000 ppm, or 1500 ppm IBA for camellia or magnolia. To serve as an industry control, the basal end of cuttings was immersed for 3-s in a solution of either 250 ppm, 4000 ppm or 2500 ppm for rose, camellia, or magnolia, respectively. A foliar application of 1500 ppm after sticking was as effective as the basal quick-dip for cuttings of ‘Southern Charm’, while other spray treatments were less effective. A basal quick-dip was more effective than a foliar spray for rooting cuttings of camellia. Auxin rate had no impact on rooting of Red Cascade™ miniature rose. The goal of commercial plant propagation is to produce high-quality rooted cuttings as quickly as possible. Plant propagation places a large demand on labor within the nursery industry, with one recent report being that labor accounts for >50% of a nursery’s budget. Our results from this trial affirm the results reported by similar trials into foliar applications of auxin suggests that the benefits of foliar applications are species dependent Further work is warranted on examining other auxin and surfactant formulations.