The effect of organic matter contained in a biosolid and chicken manure on the establishment and performance of Trichoderma spp. used as biocontrol agents against Sclerotinia spp. in lettuce (Lactuca sativa L. cv. Buba) was evaluated. Population dynamics of Trichoderma spp. was evaluated by the method of counting colony forming units on plaques of Potato-Dextrose-Agar (PDA), and its efficiency to control the disease was measured by visual assessment of the degree of crop damage by Sclerotinia spp. Statistically significant differences observed in population dynamics of Trichoderma spp. were attributed to the influence of the source of the organic matter. The biocontrol agent Trichoderma lignorum was able to use the organic matter of the biosolid and in situ organic matter. Poultry manure and the biosolid were not efficient enough to promote population growth of native Trichoderma species. The damage caused by Sclerotinia spp. was statistically similar in all treatments and less than 25 %, so that the efficiency of T. lignorum and T. harzianum to control the pathogen was proven.