Precision fertilization is a promising mitigation strategy to reduce environmental impacts of N-fertilization, but the effective benefits of variable-rate fertilization have not yet been fully demonstrated. We evaluated the short-term response (23 days) of GHGs emissions following variable-rate fertilization on barley. Yields, biomass (grains + straw) and different N-use indicators (N uptake, grain protein concentration, recovery efficiency, physiological efficiency, partial factor productivity of applied nutrient, agronomic efficiency and N surplus) were compared. Four N fertilization treatments were performed: (i) conventional– 150 kg ha−1; (ii) variable with granular fertilizer; (iii) variable with foliar liquid supplement; (iv) no fertilization. According to proximal sensing analysis (Greenseeker Handheld) and crop needs, both variable-rate treatments accounted for 35 kg N ha−1. Cumulative GHGs emissions were not significantly different, leading to the conclusion that the sensor-based N application might not be a GHGs mitigation strategy in current experimental conditions. Results showed that both site-specific fertilizations ensured the maintenance of high yields with a significant N rate reduction (approximately by 75%) and a N use improvement. Variable-rate N fertilization, due to similar yields (~6 tons ha−1) than conventional fertilization and higher protein content in foliar treatment (14%), confirms its effectiveness to manage N during the later phases of growing season.