Prolonged Feeding Research Articles

Metabolic programming and monitoring tools in pre-weaned dairy calves

The current knowledge on the effects of nutritional and environmental factors in the late embryonic and neonatal phase of dairy calves on performance later in life is summarised. The importance of epigenetic factors is increasingly recognised in this context. Prolonged colostrum feeding, discouraged on most farms to prevent the spread of Johne's disease, can have beneficial effects on the calf's immune system and has been shown to give protection against rotavirus. The importance of colostrum hygiene for uptake of antibodies is explained. Intensive milk or milk replacer feeding leads to a more productive animal while reducing the rearing costs. Iron deficiency anaemia is a common condition in whole milk fed dairy calves, and monitoring haemoglobin and supplementing iron where necessary leads to higher growth rates in calves. Other monitoring tools like lung ultrasound scanning, and beta-hydroxy-butyrate testing at weaning are briefly introduced. In conclusion, the benefits of many early intervention measures are likely to be underestimated.

Risk Factors of Early Childhood Caries Among Preschool Children in Eastern Saudi Arabia.

This study aimed to determine the risk factors of early childhood caries (ECC) among preschool children from eastern Saudi Arabia. In this cross-sectional study, 241 carers and their children from 10 daycares were recruited and asked to complete a questionnaire aimed at assessing their sociodemographic profile and oral health-related behaviors. This was followed by a dental examination of the children by two calibrated dentists. Caries status was recorded using the dmft index. Chi-square and logistic regression tests were used to analyze the data (p < 0.05). The mean dmft of the children was 4.39 (SD ± 4.25). According to logistic regression analysis, children who were bottle-fed between 7 and 12 months (Adjusted Odds Ratio (AOR): 0.110) or breastfed between 13 and 18 months (AOR: 0.028) were less likely to have ECC than those with a prolonged feeding duration (>18 months). Also, those with smoking carers (AOR: 0.176) were less likely to have ECC than those with nonsmoking carers. On the other hand, children who had working carers, mainly mothers, in the education sector (AOR: 11.105), were more likely to have ECC (p < 0.05). The risk factors associated with the presence of ECC among preschool children in eastern Saudi Arabia include the mother's occupation and the carer's smoking status, as well as feeding practices (particularly bottle feeding). These factors can be modified by encouraging carers to completely wean their children (particularly from the bottle) by 18 months of age and advising working mothers to perform tooth brushing for their children in the morning before they go to work and in the evening before the children go to bed.

New Feeding Assessment Scale for individuals with genetic syndromes: Validity and reliability in the CHARGE syndrome population.

To develop a feeding scale for parents/care givers of individuals of all ages with genetic syndromes experiencing extensive feeding and swallowing problems. Second, to assess its validity and reliability in CHARGE syndrome. The new Feeding Assessment Scale (FAS) was adapted from a scale for children who need prolonged tube feeding (Paediatric Assessment Scale for Severe Feeding Problems, PASSFP). Ten parents piloted the new scale before it was sent out with the PASSFP and feeding history questions. A subset completed the new scale again 4-8 weeks later. One hundred parents of individuals with CHARGE syndrome participated from around the world. The new scale had good construct validity, with a significant effect for an increased number of feeding risk factors having higher scale scores (P< 0.001). Face validity was high, as scores significantly differed between individuals whose parents identified their feeding difficulties as very mild, mild, moderate, severe and very severe (P< 0.001). Test-retest reliability (r=0.94, P< 0.001) and internal consistency (Cronbach's alpha 0.91) were both high. There was significant convergent validity between the new scale and the PASSFP (r=-0.79, P< 0.001). This new tool is reliable and valid for parents/care givers of individuals with CHARGE syndrome. It can be used to assess the current severity of feeding difficulties and to track progress before and after treatment. It expands upon previous existing tools in that it can be used in both individuals who are not tube fed, as well as in those who are, as well as across the life-span.

Hypothalamic Microglial Heterogeneity and Signature under High Fat Diet-Induced Inflammation.

Under high-fat feeding, the hypothalamus atypically undergoes pro-inflammatory signaling activation. Recent data from transcriptomic analysis of microglia from rodents and humans has allowed the identification of several microglial subpopulations throughout the brain. Numerous studies have clarified the roles of these cells in hypothalamic inflammation, but how each microglial subset plays its functions upon inflammatory stimuli remains unexplored. Fortunately, these data unveiling microglial heterogeneity have triggered the development of novel experimental models for studying the roles and characteristics of each microglial subtype. In this review, we explore microglial heterogeneity in the hypothalamus and their crosstalk with astrocytes under high fat diet–induced inflammation. We present novel currently available ex vivo and in vivo experimental models that can be useful when designing a new research project in this field of study. Last, we examine the transcriptomic data already published to identify how the hypothalamic microglial signature changes upon short-term and prolonged high-fat feeding.

Abstract PS17-28: Abrupt involution of lactating mammary gland induces metabolic reprogramming conducive to pro-tumorigenic changes

Abstract Background: Epidemiological studies indicate that prolonged breast feeding reduces the risk of triple negative breast cancer (TNBC), which carries the worst prognosis. Prolonged breastfeeding allows gradual involution (GI) of the breast while lack of or short-term breast feeding leads to abrupt involution (AI). We developed a novel murine model mimicking AI and GI of breast, and found that GI offers better protection to mammary glands from tissue remodeling associated injuries. Our data showed that AI leads to the development of pro-tumorigenic microenvironment and ductal hyperplasia1. Tissue remodeling involves orchestrated cell death and repopulation, and is closely associated with metabolic reprogramming from mitochondrial oxidative phosphorylation (OXPHOS) to glycolysis. Such metabolic alterations can contribute to cellular changes aiding malignant transformation2. We used our murine model to evaluate whether AI affects cellular metabolism differentially when compared to GI. Methods: Wild-type mice of FVB background were used in all our experiments. Twelve to fourteen week old uniparous mice were allowed to nurse (6 pups/dam) for 7 days postpartum. All pups were removed on day7 postpartum from the dams in AI cohort and three each on day28 and day31 from dams in GI cohort. Whole mammary glands and sorted luminal progenitor (LP) cells harvested on postpartum day28 were subjected to Affymetrix microarray analysis. Gene Set Enrichment Analysis (GSEA) was used to compute pathway enrichments in AI vs. GI glands. Differentially expressed genes were validated using qRT-PCR. Mammary glands harvested on day28 postpartum were subjected to mass spectrometry based untargeted metabolic profiling using Agilent QTOF. Raw data were analyzed using XCMS to assess key metabolic networks altered in AI vs GI glands. Targeted analysis for lactate, pyruvate, succinate and palmitic acid were performed using C13 labelled internal standards to compare OXPHOS vs glycolysis reliance in the AI and GI glands. Results: We observed enrichment of mitochondrial OXPHOS pathway, fatty acid metabolism and Myc target genes in both whole mammary gland and LP cells of AI vs. GI mice. Adipogenesis and hypoxia related genes were enriched in AI-glands. We observed significant upregulation of genes involved in glucose transport and fatty acid synthesis in AI glands, namely, Glut-5, Cidea, Acss2, Acsm3, Acly, Atp6v0d2, Acot11, and Elvol3. Several factors indicating a higher reliance on OXPHOS vs glycolysis, such as, Ppar-γ, Pgc1α, Cpt-2, Srebp1c and Chrebp were upregulated in AI glands. Upregulation of Cpt-2 and Srebp1c in the AI glands indicate higher flux through fatty acid oxidation and reliance on cholesterol synthesis. Metabolomic profiling revealed significant alteration in L-carnitine, GMP and XMP in AI glands which reflect mitochondrial fatty acid transport and nucleotide biosynthesis via guanine-guanosine salvage pathway. Pyruvate and lactate associated with glycolysis were increased in GI vs. AI glands. Conclusion: We show for the first time that in the abruptly involuting (AI) mammary glands following short-term breast feeding, there is a significant shift in the metabolic pathways towards mitochondrial OXPHOS and fatty acid oxidation compared to GI glands. Studies are underway to determine the effect of this metabolic shift on cellular transformation and tumorigenesis and the potential to target these pathways to reverse the detrimental effects of AI. 1. Basree MM, Shinde N, Koivisto C, et al. Breast Cancer Res. 2019; 21(1):80. 2. Ward PS, Thompson CB. Cancer Cell. 2012; 21(3):297. Citation Format: Neelam Shinde, Kirti Kaul, Allen Zhang, Saba Mehra, Resham Mawalkar, Hee Kyung Kim, Ramesh Ganju, Sarmila Majumder, Bhuvaneswari Ramaswamy. Abrupt involution of lactating mammary gland induces metabolic reprogramming conducive to pro-tumorigenic changes [abstract]. In: Proceedings of the 2020 San Antonio Breast Cancer Virtual Symposium; 2020 Dec 8-11; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2021;81(4 Suppl):Abstract nr PS17-28.

Consumer perception of sustainable practices in dairy production

Home-grown protein crops as an alternative to soya in dairy cattle meals, as well as other sustainable ethical-based practices, have been proposed to increase the sustainability of dairy production. Data on consumer acceptance of the three novel sustainable production strategies of ‘agroforestry’, ‘prolonged maternal feeding’ of young cattle and ‘alternative protein source’ were collected through an online survey on consumer in six European Union countries: Austria, Belgium, Denmark, Finland, Italy and the UK. Using Chen’s extended version of the Theory of Planned Behaviour model, the underlying model hypotheses on the attitudes and intentions of these consumers towards these production practices were tested, to establish the explanatory power of the model in the specific context of novel sustainable production strategies. Furthermore, the influence of gender and consumer ethical choices on their attitudes towards these innovative practices was also tested. These data show that ‘prolonged maternal feeding’ is the novel production practice that has the highest level of acceptance by consumers in all of these countries, with the least accepted practice as ‘alternative protein source’. Unexpectedly, increased availability of home-grown feed, which is grounded on both farmer and societal interests for higher input self-sufficiency and more sustainable production practices, was little appreciated by consumers, although their intentions appear to be dependent on their moral norms.

Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms.

Stable atherosclerotic plaques are characterized by a thick extracellular matrix (ECM)-rich fibrous cap populated by protective ACTA2+ myofibroblast (MF)-like cells, assumed to be almost exclusively derived from smooth muscle cells (SMC). Herein, we show that in murine and human lesions, 20 to 40% of ACTA2+ fibrous caps cells, respectively, are derived from non-SMC sources, including endothelial cells (EC) or macrophages that have undergone Endothelial-to-Mesenchymal (EndoMT) or Macrophage-to-Mesenchymal (MMT) transitions. In addition, we show that SMC-specific knockout of the platelet derived growth factor receptor beta (PDGFRB) in Apoe−/− mice fed a Western diet (WD) for 18 weeks resulted in brachiocephalic artery (BCA) lesions nearly devoid of SMC but with no changes in lesion size, remodeling, or indices of stability including percent of ACTA2+ fibrous cap cells. However, prolonged WD feeding of SMC-PDGFRB knockout mice resulted in reduced indices of stability, indicating that EndoMT and MMT-derived MFs cannot compensate indefinitely for loss of SMC-derived MFs. Using single cell and bulk RNA-seq analyses of the BCA region and in vitro models, we provide evidence that SMC to MF transitions (SMC-MFT) are induced by PDGF and TFGβ and dependent on aerobic glycolysis, while EndoMT is induced by IL1β and TGFβ. Together, we provide evidence that the ACTA2+ fibrous cap originates from a tapestry of cell types, which transition to an MF state through distinct signaling pathways that are either dependent on or associated with extensive metabolic reprogramming.

Changing the Recipe: Pathogen Directed Changes in Tick Saliva Components.

Ticks are obligate hematophagous parasites and are important vectors of a wide variety of pathogens. These pathogens include spirochetes in the genus Borrelia that cause Lyme disease, rickettsial pathogens, and tick-borne encephalitis virus, among others. Due to their prolonged feeding period of up to two weeks, hard ticks must counteract vertebrate host defense reactions in order to survive and reproduce. To overcome host defense mechanisms, ticks have evolved a large number of pharmacologically active molecules that are secreted in their saliva, which inhibits or modulates host immune defenses and wound healing responses upon injection into the bite site. These bioactive molecules in tick saliva can create a privileged environment in the host’s skin that tick-borne pathogens take advantage of. In fact, evidence is accumulating that tick-transmitted pathogens manipulate tick saliva composition to enhance their own survival, transmission, and evasion of host defenses. We review what is known about specific and functionally characterized tick saliva molecules in the context of tick infection with the genus Borrelia, the intracellular pathogen Anaplasma phagocytophilum, and tick-borne encephalitis virus. Additionally, we review studies analyzing sialome-level responses to pathogen challenge.

Technical feed quality influences health, digestion patterns, body mineralization and bone development in farming of the stomachless cleaner fish ballan wrasse (Labrus bergylta)

Farmed ballan wrasse (Labrus bergylta) is an efficient cleaner fish used for non-medicinal delicing of Atlantic salmon in sea cages replacing to an increasing degree wild wrasse due to considerations for biodiversity and risk of overfishing local wrasse populations. Farming of ballan wrasse has been hampered by low growth rates, high prevalence of skeletal deformities and other welfare related pathologies. In this study we investigated how diets identical in composition but differing in their technical characteristics, by being prepared using different feed production technologies, affect fish performance, mineralization, bone development and gut health of the ballan wrasse larvae and juveniles. The different production technologies include the commonly used ‘high temperature’ extrusion, cold extrusion, and agglomeration, resulting in feed pellets with distinctive physicochemical properties. The results revealed that prolonged feeding periods with extruded pellets during ballan wrasse larvae weaning result in low body mineralization and the development of severe skeletal deformities. In juvenile ballan wrasse, the extruded pellet treatment resulted in higher mortality rates, fish with larger livers, indication for increased serum TAG and cholesterol in a similar manner, and increased activity of the digestive enzymes LAP and maltase, most probably as a compensatory mechanism to the assumed reduced availability of protein and carbohydrates of extruded pellets for this fish species. Smaller dietary effects were identified in terms of intestinal morphology and gene transcription rates.

INCLUSION RATE AND PHYSIOLOGICAL EFFECTS OF PROLONGED FEEDING OF COCOA BEAN CAKE ON LAYERS

The effect of inclusion of cocoa bean cake (CBC) on productive performance and physiological response of Isa Brown pullets to prolonged feeding of CBC were investigated. Dietary inclusion of CBC (0, 50, 100 and 200g kg-1 diet) were monitored in typical poultry diet from day old to 51 weeks of age. Sexual maturity and attained of 50% hen-day production were delayed with increasing level of CBC. Egg quality indices were higher (P &lt; 0.05) at 200g kg-1 CBC diet but with reduced (P &lt; 0.05) shell thickness. CBC had no significant influence on yolk or whole egg cholesterol, follicular weight and number. Layers on 0 and 50 kg-1 CBC diets had higher (P &lt; 0.05) percentage of yellow follicles but lower white follicles. Liver and pancreatic hypertrophy were observed for CBC-fed layers but with reduced (P &lt; 0.05) abdominal fat and oviduct length. The long term feeding of CBC at level more than 50g kg-1 diet progressively had a deteriorating effect on laying performance and follicular development.

Eating and swallowing disorders in children with cerebral palsy

Introduction. Cerebral palsy is characterized by abnormalities of muscle tone, movement and motor abilities caused by disorders in brain development. Many children with cerebral palsy have severe swallowing problems. Cerebral Palsy. Cerebral palsy is a neurodevelopmental disorder characterized by abnormalities in brain development. Swallowing is a process by which a bolus that is formed within the oral cavity is transported through the pharynx into the esophagus and stomach. It has four phases: preparatory oral, oral, pharyngeal and esophageal. Swallowing disorders in children with cerebral palsy. Swallowing disorders vary depending on the type of cerebral palsy. Children with spastic paralysis have a more pronounced disorder of the voluntary phases of swallowing, while children with athetoid paralysis are expected to have a disorder at the oral-motor level. Neurological lesions may affect the muscle function of the jaw, cheeks, lips, tongue, palate and pharynx, which is manifested by difficulties in controlling saliva and swallowing. Diagnosis and treatment of dysphagia in cerebral palsy. Diagnosis and treatment of dysphagia is best accomplished by a team of experts including a pediatrician, neurologist, otorhinolaryngologist, gastroenterologist, radiologist, dental specialist, speech therapist, nutritionist, and a specialist in physical medicine and rehabilitation. Conclusion. Dysphagia is a common comorbidity in children with cerebral palsy. Although swallowing disorders often correlate with the type of cerebral palsy, the most prevalent signs of dysphagia include: coughing, prolonged feeding time, laryngotracheal penetration and aspiration, suffocation, which leads to dehydration, malnutrition, but also to the need for alternative feeding methods. It is important to emphasize the importance of early treatment by a team of experts, with a speech therapist as a team leader.

Obesity in Midlife Hampers Resting and Sensory-Evoked Cerebral Blood Flow in Mice.

This study aimed to investigate the effects of a high-fat diet (HFD) and aging on resting and activity-dependent cerebral blood flow (CBF). To run a comparison between obese and age-matched control animals, 6-week-old mice were fed either with regular chow or an HFD for 3 months or 8 months. Glucose tolerance and insulin sensitivity were assessed for metabolic phenotyping. Resting and odor-evoked CBF at the microvascular scale in the olfactory bulb (OB) was investigated by multiexposure speckle imaging. Immunolabeling-enabled imaging of solvent-cleared organs was used to analyze vascular density. The ejection fraction was studied by using cardioechography. Olfactory sensitivity was tested by using a buried-food test. Glucose intolerance and compromised odor-evoked CBF were observed in obese mice in the younger group. Prolonged HFD feeding triggered insulin resistance and stronger impairment in activity-dependent CBF. Aging had a specific negative impact on resting CBF. There was no decrease in vascular density in the OB of obese mice, although cardiac function was impaired at both ages. In addition, decreased olfactory sensitivity was observed only in the older, middle-aged obese mice. OB microvasculature in obese mice showed a specific functional feature characterized by impaired sensory-evoked CBF and a specific deleterious effect of aging on resting CBF.

Cultivation of aerobic granular sludge for the treatment of food-processing wastewater and the impact on membrane filtration properties.

A laboratory-scale sequencing batch reactor was operated for approximately 300 days, divided into four periods based on the feeding strategy, to develop stable aerobic granular sludge (AGS) while treating chocolate processing wastewater. Application of a prolonged mixed anaerobic feeding was not sufficient to develop AGS and reach stable reactor performance. Through the application of a partially non-mixed and a partially mixed feeding strategy, the reactor performance was increased and stable AGS formation was established characterized by low diluted sludge volume index (D)SVI DSVI10,30) values of 78 ± 27 mL·g-1 and 52 ± 17 mL·g-1, respectively, and a capillary suction time/mixed liquor suspended solids value of 0.9 sec·(g·L-1)-1. The membrane bioreactor (MBR) filtration tests showed a reduction of the fouling rate (FR) and an increase of the sustainable flux (SF0.5) for AGS compared to flocs treating the same industrial wastewater. The SF0.5 (FR > 0.5 mbar·min-1) for the flocs was 10 L·(m2·h)-1 while for AGS the SF0.5 is higher than 45 L·(m2·h)-1 because the FR did not exceed 0.1 mbar·min-1. Additionally, the AGS showed reduced irreversible fouling tendencies due to pore blocking. Our results underline the need for an increased substrate gradient during anaerobic feeding for the development and long-term maintenance of AGS under minimum wash-out conditions. The AGS-MBR filtration performance also shows strong advantages compared to a floccular MBR system due to a high increase of the SF0.5 and reduced reversible and irreversible fouling.

Types of Parasitism of Hard Ticks (Ixodidae)

Critical revision of the terms “pasture (non-nidicole) type of parasitism” and “nest-and-burrow (nidicole) type of parasitism” widespread in the literature forces us to abandon these terms. All the hard ticks are characterized by the same type of parasitism: they belong to a group of temporary ectoparasites with prolonged feeding (Balashov, 2009). By the type of habitat of non-parasitic life stages these parasites can be subdivided into ecological groups of nidobionts (dwellers of nests and burrows) and nemobionts (comprising ticks dwelling outside nests and burrows at non-parasitic life stages).

Prolonged prepregnant maternal high-fat feeding reduces fetal and neonatal blood glucose concentrations by enhancing fetal β-cell development in C57BL/6 mice

To read the full abstract: Diabetes. 2019 Aug;68(8):1604–1613. doi: 10.2337/db18-1308. Epub 2019 May 24. PMID: 31127056

Button Gastrostomy Tubes for Pediatric Patients: A Tertiary Care Center Experience.

Results Out of 34 patients who underwent gastrostomy tube insertion, 30 patients had their long tube replaced by a button gastrostomy. Majority were males (N = 18, 60%). Prolonged nasogastric tube feeding was the main indication of referral (N = 17, 56%) followed by feed intolerance (N = 6, 17%) and gastroesophageal reflux disease (N = 5, 16%). The main underlying diseases at referral were neurological impairment (N = 19, 63%) and metabolic disorders (N = 4, 13%). There was no significant difference between patients with neurological disorders and other diseases in terms of gender, nationality, or age. Laparotomy with gastrostomy is the main approach used (N = 18, 60%). No reported complications of button tubes in 50% of the patients (N = 15). Conclusions Prolonged nasogastric tube feeding is the main indication of referral for gastrostomy tube insertion. Neurological disorders are the main diagnosis for the cases operated upon. Laparotomy with gastrostomy is the procedure of choice at our center. Majority of patients had no reported complications of button tube replacement. These children are likely to benefit from the button tube with fewer complications.

About castration in osteomalacia

Osteomalacia is mainly reduced to changes in the bones - they lose their mineral substances and along with this, abrupt changes occur in the organic structure of bones and in the bone marrow - and the bone and bone marrow soften. Along with this, degenerative changes occur in the muscular system. The clinical picture is first expressed by pain in different parts of the skeleton, early fatigue, increased reflexes; then, with strong development, bone curvature, persistent catarrh of the respiratory and digestive tract; patients die with symptoms of insanity. The disease lasts from 1 to 10 years and affects mainly women who have already given birth. Neither poverty nor prolonged feeding seems to matter.

Effects of feeding graded levels of whole cottonseed on haemato-biochemical parameters of red Sokoto bucks

Whole cottonseed (WCS) is an important source of protein for ruminants, however, it contains a polyphenolic secondary metabolite gossypol which may reduce its palatability and cause pathophysiological effects. The study was aimed at evaluating the effects of feeding graded levels of WCS on the haemato-biochemical parameters of Red Sokoto bucks (RSB). After a 14-day pre-treatment period, 20 bucks (n = 5) were assigned for 90 days to one of four isonitrogeneous treatments: control (A); 0 % (B); 15 % (C); 30 % and (D); 45 %. Blood was collected for Packed Cell Volume (PCV) and serum analyses at days 0, 45 and 90 of the experimental period for concentrations of urea, creatinine, total protein, albumin, globulin and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP). The PCV was decreased in groups fed above 15 % of WCS on days 45. There were no significant (P &gt; 0.05) differences in globulin concentration, alanine aminotransferase and alkaline phosphatase activities but aspartate transferase activity was higher (p &lt; 0.05) in group D (45% WCS) compared to the various treatment groups. The urea concentration was higher (p &lt; 0.05) in groups B and D at day 45 compared to the control group. The creatinine concentration was higher (p &lt; 0.05) in group D at days 45 and 90 compared to the control group. The serum total protein and albumin concentration were higher (p &lt; 0.05) in groups C and D at day 45 compared to the control group. Our finding revealed that prolonged feeding of bucks above 30 % WCS for more than 60 days exerted considerable deleterious and adverse effects on blood constituents leading to aneamia, hyper-cretenaemia, ureamia, increased levels of total protein, albumin, aspartate transferase activity and death.&#x0D; Keywords: Biochemical parameters, Bucks, Gossypol, Serum, Whole cotton seed

Temporary feeding tube dependency in pediatric patients: A retrospective analysis of risk factors and preventative practices

Prolonged temporary tube feeding places pediatric patients at risk of tube dependency. This chart audit aimed to identify current temporary tube feeding practice within an Australian tertiary hospital, risk factors of prolonged temporary tube feeding, and the likelihood of high risk patients receiving tube dependency preventative practices. A retrospective chart audit was conducted of the medical records of 187 pediatric inpatients who had temporary feeding tubes placed in an Australian tertiary hospital between November 2014 and March 2017. Information was collected on patient demographics and tube feeding practices. The Kaplan Meier estimate, Cox regression and logistic regression were used to determine the median time until feeding tube removal, predictors of prolonged temporary tube feeding, and the relationship between these predictors and utilization of preventative practices. The Kaplan Meier estimate of median tube feeding duration was 6.43 months (95% CI: 5.17-7.90). Predictors of prolonged tube feeding were prematurity (p=0.003), feeding difficulties requiring speech pathology referral (p=0.002), and referral for long-term feeding tubes (p=0.002). There was a low prevalence of preventative clinical practices including documentation of tube exit plans (5.3%, n=10) and referral for long-term feeding tubes (27.3%, n=51). Exhibiting risk factors for prolonged tube feeding did not increase the likelihood of receiving preventative clinical practices. The prolonged duration of temporary tube feeding within this sample highlights the need for improved utilization of tube dependency preventative practices within high risk groups.

VMAT2 Safeguards β-Cells Against Dopamine Cytotoxicity Under High-Fat Diet-Induced Stress.

Vesicular monoamine transporter 2 (VMAT2) uptakes cytoplasmic monoamines into vesicles for storage. VMAT2 plays a role in modulating insulin release by regulating dopamine levels in the pancreas, although the exact mechanism remains elusive. We found that VMAT2 expression in β-cells specifically increases under high blood glucose conditions. The islets isolated from β-cell–specific Vmat2 knockout (βVmat2KO) mice show elevated insulin secretion levels in response to glucose stimulation. Under prolonged high-fat diet feedings, the βVmat2KO mice exhibit impaired glucose and insulin tolerance and progressive β-cell dysfunction. Here we demonstrate VMAT2 uptake of dopamine to protect dopamine from degradation by monoamine oxidase, thereby safeguarding β-cells from excess reactive oxygen species (ROS) exposure. In the context of high demand for insulin secretion, the absence of VMAT2 leads to elevated ROS in β-cells, which accelerates β-cell dedifferentiation and β-cell loss. Therefore, VMAT2 controls the amount of dopamine in β-cells, thereby protecting pancreatic β-cells from excessive oxidative stress.